JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 11
·112508
·
IF 3.721
Kui Liu,Wei Wang,Yue Ma,Mingliang Kang,Biao Wang
In this work, the diffusion coefficient and electrocrystallization of yttrium in LiCl-KCl eutectic are typically investigated using different electrochemical techniques such as cyclic voltammetry (CV), chronopotentiometry (CP) and chronoamperometry (CA) techniques. At temperature from 673 to 823 K, the diffusion coefficients of two yttrium concentrations (YCl3 = 3.816 × 10−5 and 9.981 × 10−5 mol ml−1) were determined by CP and CV methods in a range from 0.453 \times 10−5 to 8.25 \times 10−5 cm2 s−1. The nucleation of Y electrodeposition is confirmed to be an instantaneous mode regardless the given temperature, over-potential and concentrations. The morphology of yttrium nuclei and growth evolution on a tungsten surface were characterized by the SEM-EDS. Some individual circular regions corresponding to initial Y nucleation sites are firstly observed on the tungsten surface. The subsequent formed Y nuclei were found to migrate toward those circular nucleation sites to extend its growth on the inside and peripheral of the circle. In addition, no distinct dendritic structure for the Y deposition was observed on the W surface.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
ACS Synthetic Biology
·
2023
·
IF 4.7
Peng Shen,Xiaojun Qu,Qinyu Ge,Ting Huang,Qingjiang Sun,Zuhong Lu
DNA is an attractive medium for long-term data storage because of its density, ease of copying, sustainability, and longevity. Recent advances have focused on the development of new encoding algorithms, automation, and sequencing technologies. Despite progress in these subareas, the most challenging hurdle in the deployment of DNA storage remains the reliability of preservation and the repeatability of reading. Herein, we report the construction of a magnetic bead spherical nucleic acid (MB-SNA) composite microstructure and its use as a cost-effective platform for reliable DNA preservation and repeated reading. MB-SNA has an inner core of silica@γ-Fe2O3@silica microbeads and an outer spherical shell of double-stranded DNA (dsDNA) with a density as high as 34 pmol/cm2. For MB-SNA, each strand of dsDNA stored a piece of data, and the high-density packing of dsDNA achieved high-capacity storage. MB-SNA was advantageous in terms of reliable preservation over free DNA. By accelerated aging tests, the data of MB-SNA is demonstrated to be readable after 0.23 million years of preservation at −18 °C and 50% relative humidity. Moreover, MB-SNA facilitated repeated reading by facile PCR-magnetic separation. After 10 cycles of PCR access, the retention rate of dsDNA for MB-SNA is demonstrated to be as high as 93%, and the accuracy of sequencing is more than 98%. In addition, MB-SNA makes cost-effective DNA storage feasible. By serial dilution, the physical limit for MB-SNA to achieve accurate reading is probed to be as low as two microstructures.
研究领域:
生物化学
·
分子生物学
·
数据科学
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DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Xiaoning Liang,Chaoman He,Hong Shen
Neutrophils play a pivotal role in innate immunity by releasing ROS through respiratory bursts to neutralize various pathogenic factors. However, excessive ROS release can cause tissue damage. Adenosine is an endogenous anti-inflammatory molecule inhibiting respiratory burst to protect the host. Adenosine aptamers with antibody-like properties and good stability are expected to act as adenosine antagonists with functional modulation capability. This study compares the effects of adenosine and its aptamer on the respiratory bursts of salivary polymorphonuclear leukocytes and circulating polymorphonuclear leukocytes using a programmable stopped-flow injection approach, ensuring rapid and efficient analysis while maintaining the neutrophils’ viability. The results show that primed salivary polymorphonuclear leukocytes exhibit specificities that differ from circulating polymorphonuclear leukocytes. Adenosine aptamer can function as an inhibitory antagonist that distinguishes between physiologically controlled and excessive priming of neutrophils, showing potential application prospects in immunotherapy.
研究领域:
免疫学
·
生物化学
·
药理学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Ming-Chao Jiang,Zhou-Long Fang,Jin-Yan Zhang,Wei Ma,Luan-Feng Liao,Cui-Yun Yu,Hua Wei
Facile construction of a fully biodegradable spherical nucleic acid (SNA) nanoplatform is highly desirable for clinical translations but remains rarely explored. We developed herein the first polycarbonate-based biodegradable SNA nanoplatform for self-codelivery of a chemotherapeutic drug, doxorubicin (DOX), and a human liver-specific miR122 for synergistic chemo-gene therapy of hepatocellular carcinoma (HCC). Ring-opening polymerization (ROP) of a carbonate monomer leads to a well-defined polycarbonate backbone for subsequent DOX conjugation to the pendant side chains via acidic pH-cleavage Schiff base links and miR122 incorporation to the chain termini via click coupling, affording an amphiphilic polycarbonate-DOX-miR122 conjugate, PBis-Mpa 30 -DOX-miR122 that can self-assemble into stabilized SNA. Besides the desired biodegradability, another notable merit of this nanoplatform is the use of miR122 not only for gene therapy but also for enhanced innate immune response. Together with the ICD-triggering effect of DOX, PBis-Mpa 30 -DOX-miR122 SNA-mediated DOX and miR122 codelivery leads to synergistic immunogenicity enhancement, resulting in tumor growth inhibition value (TGI) of 98.1 % significantly higher than those of the groups treated with only drug or gene in a Hepa1-6-tumor-bearing mice model. Overall, this study develops a useful strategy toward biodegradable SNA construction, and presents a drug and gene-based self-codelivery SNA with synergistic immunogenicity enhancement for efficient HCC therapy. Statement of significance Facile construction of a fully biodegradable SNA nanoplatform is useful for in vivo applications but remains relatively unexplored likely due to the synthetic challenge. We report herein construction of a polycarbonate-based SNA nanoplatform for co-delivering a chemotherapeutic drug, DOX, and a human liver-specific miR-122 for synergistic HCC treatment. In addition to the desired biodegradability properties, this SNA nanoplatform integrates DOX-triggered ICD and miR-122-enhanced innate immunity for simultaneously activating adaptive and innate immunities, which leads to potent antitumor efficiency with a TGI value of 98.1 % in a Hepa1-6-tumor-bearing mice model.
研究领域:
肿瘤学
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分子生物学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yinghua Zhu,Youduo Zhao,Jingjing Deng,Tianshu Zhou
The widespread application of neomycin (NEO) has raised pollution problem caused by the accumulation of its residues, which poses potential risk to human health and ecological safety. Therefore, the development of sensitive and reliable approaches is crucial for detecting and monitoring low levels of NEO in complex samples. In this study, an innovative spherical nucleic acid (SNA) based sensor was designed for the selective detection of NEO. The colorimetric response was attributed to hydrogen bonding, electrostatic interaction, and coordination between the SNA and NEO. Meanwhile, the fluorescence of FAM-labeled SNA was quenched by NEO due to fluorescence resonance energy transfer (FRET) effect. By integrating visual colorimetry and fluorescence analysis, the dual-responsive sensor enabled rapid, selective, and sensitive determination of NEO, with detection limits of 40.0 nM and 3.3 nM, respectively. Furthermore, the SNA exhibited good salt tolerance, maintaining stability without aggregation even in high-salt conditions. The average recoveries for detecting NEO in water samples analysis ranged from 97.6 % to 108.7 %, confirming its reliability and accuracy in practical applications. This work provides an effective strategy for NEO detection and expands the potential applications of SNA in environmental monitoring and pollution control.
研究领域:
生物化学
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环境科学
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分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Kai-Ren Zhao,Li Wang,Peng-Fei Liu,Xiao-Min Hang,Hui-Yi Wang,Shu-Ying Ye,Zhi-Jun Liu,Guo-Xi Liang
Herein, we report a “on-off” signal-switchable electrochemiluminescence (ECL) biosensor for simple, sensitive and quantitative detection of two kinds of virus genes, human immunodeficiency virus (HIV) and human papilloma virus (HPV-16) DNAs, by the integration of spherical nucleic acid (SNA) with CRISPR/Cas12a. The concept behind this approach is “on” signal based on a sandwich type assay after ECL luminophores of carbon dots (CDs) labeled SNA incubated with the biosensor in the presence of HIV DNA; and “off” signal upon the biosensor incubation with assistant DNA (HIV DNA sequence), CDs-labeled SNA and Cas12a/crRNA/HPV-16 DNA ternary complex relying on the collaterally cleavage of Cas12a. The cross-interference between HIV and HPV-16 DNAs can be avoided by adding Cas12a effector to the system or not. Using this strategy, HIV and HPV-16 DNAs are successfully quantitatively detection with detection limits of 30 fM and 0.32 pM (S/N = 3), respectively. Importantly, this system does not rely on multiplex signal reporting molecules, thus simplifying the detection system and avoiding the likelihood of cross interferences presented in the assay. Furthermore, multiplex detection of HIV/HPV-16 DNAs in human serum samples are successfully achieved within 2 h without additional nucleic acid amplification step. As demonstrated, the proposed biosensor has the potential for multiplex detection in clinical diagnosis.
研究领域:
生物传感技术
·
分子诊断学
·
病毒学
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产品关联
DOI
高影响因子
POLYMER TESTING
·
2020
·
IF 3.275
Lei Xu,Zhengliang Huang,Yao Yang,Binbo Jiang,Jingyuan Sun,Jingdai Wang,Yongrong Yang
High density polyethylene/n-Hexane system was adopted to investigate the bubbles nucleation and collapse in polymer melt with a visualization apparatus at different temperatures. The results showed that the initial bubble number, initial total bubble volume and the mean bubble diameter all increased with the temperature increasing, and the bubble diameter distribution changed from single peak distribution to bimodal distribution. Moreover, the effect of temperature on the maximum bubble diameter was mainly reflected in the change of the pressure in bubbles due to the change of the volatile content in the polyethylene melt. For bubble collapse, the collapse time became longer but the bubble collapse rate became faster with the temperature increasing, and a fitting model was established in which the collapse coefficient n was used to describe the collapse rate. Through dimension analysis, the correlation between the collapse coefficient n and the melt surface tension σ , zero-shear melt viscosity η 0 , diffusion coefficient D , maximum bubble diameter d max , melt density ρ , initial bubble number density N ini were established and the variation of D with temperature could be predicted as an exponential correlation.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-15
·
IF 15.7
Liu Wenjia,Liu Yuting,Zhang Liqiang,Li Liya,Yang Wenguang,Li Jia,He Wangxiao
Liver sinusoidal endothelial cells (LSECs) lose their characteristic fenestrations and become capillarized during the progression of liver fibrosis. Mesenchymal stem cell (MSC) transplantation can reverse this capillarization and reduce fibrosis, but MSC therapy has practical limitations that hinder its clinical use. Here, with the help of artificial intelligence (AI), we show that MSCs secrete a microRNA (miR-325-3p) that helps restore LSEC fenestrations (tiny pores) by modulating their cytoskeleton, effectively reversing capillarization. We further develop a spherical nucleic acid (SNA) nanoparticle carrying miR-325-3p as an alternative to MSC therapy. This SNA specifically enters fibrotic LSECs via the scavenger receptor A (Scara). In three mouse models of liver fibrosis, the SNA treatment restores LSEC fenestrations, reverses capillarization, and significantly reduces fibrosis without adverse effects. Our findings highlight the potential of SNA-based therapy for liver fibrosis, paving the way for targeted nucleic acid treatments directed at LSECs and offering hope for patients.
研究领域:
分子生物学
·
肝脏病学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Chao Li,Long Guo,Xiaoqiang Sang,Xiaoli Jiang,Hanwen Wang,Panzhu Qin,Lin Huang
Exosomes are one of the most promising biomarkers for tumor diagnosis and prognosis. Therefore, the development of convenient and sensitive exosome sensing strategies is of great significance. Herein, we integrated aptamer-based spherical nucleic acids (SNAs) and hybridization chain reaction (HCR) into a colorimetric aptasensor platform and applied it to the detection of exosomes. In this design, the CD63-specific aptamer pre-immobilized on the microplate was used to capture target exosomes, while the SNAs conjugated with nucleolin-specific aptamer and trigger probe H1 were designed for amplifying signal. In the presence of target exosomes, the SNAs can be attached to the microplate by the bridge effect of exosomes, resulting in the trigger of HCR. This process is accompanied by the formation of abundant G-quadruplex/hemin DNAzyme, enabling the visual quantitative analysis of exosomes. Featured with the dual amplification of SNAs and HCR, the proposed aptasensor achieved a considerable detection limit of 50 particles/μL. The practicability of this method was further verified by testing the different clinical samples. Given the ability of the aptasensor to visually detect exosomes in scenarios lacking instruments and resources, we believe that the aptasensor can be serve as a potential on-site test for liquid biopsy.
研究领域:
生物化学
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医学诊断
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Sensors & Diagnostics
·
2022
·Vol. 2
·期 1
·194-202
·
IF 0
Shiyang Qu,Lin Shi,Huan Li,Tao Li
As a nano-form of G-quadruplex (G4) based DNAzymes, spherical nucleic acid enzymes (SNAzymes) with gold nanoparticles (AuNPs) as cores have shown great promise for applications in nucleic acid detection. SNAzymes can be constructed by modifying thiolated G4 DNAs onto AuNPs through various methods. However, those constructed with label-free G4 DNAs were found to have a weak enzyme activity due to the G4 destruction on the surface of AuNPs. To address this, here we constructed label-free silicon-based SNAzymes using silica nanoparticles (SiO2) as the core for the first time. We took advantage of the versatility of SiO2 and loaded N-(4-aminobutyl)-N-ethylisoluminol (ABEI) on the surface of silicon spheres (SiO2@ABEI). Then, we found that SiO2@ABEI can achieve high-efficiency adsorption of arbitrary G4 sequences within a few minutes without high requirements for modification methods. In this way, a highly sensitive CL nanoprobe integrated with a catalyst and a chemiluminescence (CL) reagent was successfully constructed. Finally, we use the nanoprobe for the ultrasensitive chemiluminescence detection of acute myocardial infarction (AMI)-related nucleic acids, achieving a detection range of 10 fM–100 pM and an excellent detection limit of 0.8 fM.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
DOI
JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 52
·期 6
·3535-3544
·
IF 2.302
Gao Xiufeng,Chen Shaoping,Dong Feng,Hu Lifang,Yang Renyao,Wang Wenxian,Munir Zuhair A.
The diffusion bonding between Ti and Ni was investigated in the presence and absence of an imposed electric current. Bonding was carried out over the temperature range of 873–1123 K and the current density range of 255–350 A/cm 2 . The formation and sequence of evolution of the three intermetallic phases, TiNi 3 , TiNi, and Ti 2 Ni, were investigated and discussed in light of calculations of Gibbs free energies of formation and interfacial energies associated with their nucleation. Within the experimental times and in the presence of a current, the growth of the three phases was parabolic. The evolution of the TiNi phase required an incubation period, while the relatively high growth rate of TiNi 3 suggests the possibility of another mechanism. The growth of all phases was enhanced by the application of a direct current, with no apparent dependence on its direction.
研究领域:
材料科学
·
冶金学
·
电化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Xiaotong Wei,Dewei Liu,Min Zhao,Tiantian Yang,Yunpeng Fan,Wenqin Chen,Ping Liu,Jianbo Li,Shijia Ding
MicroRNAs (miRNAs), considered as therapeutic targets and biomarkers, play important roles in biological processes . Herein, an enzyme-free surface plasmon resonance imaging (SPRi) biosensing method has been developed for miRNA detection based on catalytic hairpin assembly and spherical nucleic acid . The hairpin H1 tethered on the surface of the sensor chip is unfolded by miRNA, and then the hybridized miRNA is released through the displacement of the hairpin H2 for the successive hybridization and assembly process. The emerging DNA fragments on the sensor chip surface after hairpins assembly are further used to hybridize with spherical nucleic acid, inducing a remarkably amplified SPR signal. This biosensing method is highly sensitive to miRNA with a detection limit of 53.7 fM and a linear range of 4 orders of magnitude. Moreover, the biosensor demonstrates good specificity and has the ability to distinguish members of homologous miRNA family even with single base differences. Thus, the SPRi biosensing method may hold a great promise for further application in early clinical diagnosis.
研究领域:
分子生物学
·
生物传感技术
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）