CHINESE CHEMICAL LETTERS
·
2022
·
IF 8.455
Hao Gu,Guoxin Dai,Siyu Liu,Yanbing Zhai,Qian Xu,Hongqiang Wu,Wei Xu
Liquid chromatography tandem mass spectrometry (LC-MS/MS) plays an important role in clinical diagnostics. Although LC-MS/MS is superior in terms of accurately quantifying molecules in complex matrices, instrument footprint, operation and maintenance complexity also hinder its expansion as the analytical technique of choice. In this study, a compact LC-MS instrument was developed, in which an assembled liquid chromatograph was coupled with a miniature ion trap mass spectrometer. The overall instrument has a footprint of 69 cm × 31 cm × 31 cm, and it requires no gas supply as well as minimum maintenance. Furthermore, the use of LC-MS is in accord with conventional clinical diagnostic protocols, and the choice of ion trap offers tandem MS performance. The results showed that the use of LC could improve both mixture analysis capability and detection sensitivity of the miniature mass spectrometer. After optimization, feasibility of this instrument in clinical practice was demonstrated by the quantitation of four widely used immunosuppressants in blood samples. Relatively good linearities were obtained, which spanned the reference ranges of effective therapeutic concentrations of each immunosuppressant. Intra-day and inter-day accuracy and precision of analytical method were also assessed. This work showed that a compact LC-MS instrument could be used in clinical diagnosis, either to replace conventional lab-scale instruments or to be used in POCT applications.
研究领域:
分析化学
·
医学
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DOI
高影响因子
Chinese Optics Letters
·
2020
·Vol. 18
·期 10
·101202
·
IF 2.045
Hai Zhang,Jianyu Shang,Xiujuan Liu,Weimin Sun,Feiru Wang,Yongjun Liu,Yongjun Liu
This Letter presents a new type of optical fiber probe used to detect temperature, whose structure is very simple. The optical fiber probe is filled with cholesteric liquid crystals (CLCs) whose reflected light varies with temperature. The experimental results show that the proposed sensor can achieve a temperature sensitivity of 5.64 nm/°C in the temperature range of 18–40°C. The sensor has the advantages of simple structure, low cost, and easy mass manufacture. Its size is very tiny (the tapered structure, 125 μm in maximum diameter and <300 μm in length) and it is easy to integrate and measure. Meantime, the tapered structure of the probe is also ideal for measuring small samples such as cells and microfluidic channels, which will be a promising candidate for monitoring temperature fluctuations in small spaces.
研究领域:
光学
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材料科学
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生物医学工程
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高影响因子
NATURE
·
2023
·Vol. 624
·期 7990
·74-79
·
IF 64.8
Hu Yajie,Wu Mingmao,Chi Fengyao,Lai Guobin,Li Puying,He Wenya,Lu Bing,Weng Chuanxin,Lin Jinguo,Chen Fengen,Cheng Huhu,Liu Feng,Jiang Lan,Qu Liangti
Electrochemical capacitors are expected to replace conventional electrolytic capacitors in line filtering for integrated circuits and portable electronics 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 . However, practical implementation of electrochemical capacitors into line-filtering circuits has not yet been achieved owing to the difficulty in synergistic accomplishment of fast responses, high specific capacitance, miniaturization and circuit-compatible integration 1 , 4 , 5 , 9 , 10 , 11 , 12 . Here we propose an electric-field enhancement strategy to promote frequency characteristics and capacitance simultaneously. By downscaling the channel width with femtosecond-laser scribing, a miniaturized narrow-channel in-plane electrochemical capacitor shows drastically reduced ionic resistances within both the electrode material and the electrolyte, leading to an ultralow series resistance of 39 mΩ cm 2 at 120 Hz. As a consequence, an ultrahigh areal capacitance of up to 5.2 mF cm −2 is achieved with a phase angle of −80° at 120 Hz, twice as large as one of the highest reported previously 4 , 13 , 14 , and little degradation is observed over 1,000,000 cycles. Scalable integration of this electrochemical capacitor into microcircuitry shows a high integration density of 80 cells cm −2 and on-demand customization of capacitance and voltage. In light of excellent filtering performances and circuit compatibility, this work presents an important step of line-filtering electrochemical capacitors towards practical applications in integrated circuits and flexible electronics.
研究领域:
材料科学
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电子工程
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高影响因子
ELECTROANALYSIS
·
2020
·Vol. 32
·期 9
·2018-2026
·
IF 2.544
Wanlin Dai,Wenyuan Zhao,Ying Ma,Jianshan Ye,Jiye Jin
We developed a miniaturized electrochemiluminescence (ECL) instrument coupled with a light-emitting diode-based bipolar electrochemical sensor (LED-BPES). This instrument composes of a microcontroller circuit, a power supply circuit, a potentiostat, an optical detecting circuit, and a communication circuit. The multi-pixel photon counter (MPPC), which is low-cost, small-size, and wide-range in optical measurements, is chosen as the optical detector. The LED-BPES composes of a disposable screen-printed carbon electrode (SPCE) and a surface-mount red LED. Depended on the closed bipolar electrode (C-BPE) structure, the LED-BPES not only avoids the employment of unstable and complex ECL reactions but also offers a cost-effective alternative for the over-priced ECL reagents by using a mini-size commercial LED as the luminescent producer. The combination of MPPC and LED-BPES helps to set up the simplified and downsized instrument system with low price and high efficiency. The presented instrument coupled with LED-BPES works excellent in electroactive molecules detection and has great potential in the application of heavy metal ions detection.
研究领域:
分析化学
·
生物传感技术
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DOI
高影响因子
ANALYST
·
2016
·Vol. 141
·期 10
·3041-3049
·
IF 4.033
Yan Xiong,Chengjie Wang,Tao Tao,Ming Duan,Jun Tan,Jiayi Wu,Dong Wang
Fluoride concentration is a key aspect of water quality and essential for human health. Too much or too little fluoride intake from water supplies is harmful to public health. In this study, a capillary waveguide integrated fiber-optic sensor was fabricated for fluoride measurement in water samples. The sensor was modularly designed with three parts, i.e., a light source, capillary flow cell and detector. When light propagated from a light emitting diode (LED) to the capillary waveguide cell through an excitation fiber, it interacted with the sensing reagent, and its intensity changed with different fluoride concentrations. Then, the light propagated to the detector through a detection fiber for absorption determination of fluoride according to Beer's law. This miniaturized sensor showed advantages of fast analysis (9.2 s) and small reagent demand (200 μL) per sample, and it also had a low detection limit (8 ppb) and high selectivity for fluoride determination. The sensor was applied to fluoride determination in different water samples. The results obtained were compared with those obtained by conventional spectrophotometry and ion chromatography, showing agreement and validating the sensor's potential application.
研究领域:
分析化学
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环境监测
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生物医学传感
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水质安全
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光学传感器
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公共健康
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DOI
高影响因子
IEEE SENSORS JOURNAL
·
2023
·Vol. 23
·期 4
·3475-3484
·
IF 4.3
Jiawen Yin,Wei Chen,Wanlei Gao,Xin Zhang,Bo Tang,Qinghui Jin
Considering the indispensable of pH for tap water, a pH sensor with the characteristics of an implantable, long-term stable, and long lifetime is urgently needed for on-line and in situ pH monitoring in tap water, but this is always a challenging issue. In this article, a competitive integrated electrochemical pH sensor is developed and batch manufactured via micro-electro-mechanical system (MEMS). A dense and ultrasensitive IrOx electrode is obtained for almost 1.5 times Nernst response (−82.88 mV/pH), after preparation process optimization. A Si-Si3N4-KCl agar gel-polydimethylsiloxane (PDMS) multilayer structured Ag/AgCl electrode is designed and proven to be a long lifetime (>40 days) and be stable. By integrating with the IrOx film and solid-state Ag/AgCl electrode on a mini chip and verifying the effectiveness of the as-fabricated sensor by pH detection, the miniature pH sensor demonstrates wide detection range (1.8–12), stirring sensitivity, desirable linearity ( ${R}^{2}=0.999$ ), ideal repeatability and good resistance to other interference substances. Particularly, by employing the mass-production technique, favorable response consistency (relative standard deviation (RSD) < 2%) is witnessed, offering the opportunity to mass-produce the proposed sensor in the future. What’s more, the sensor still kept 91.7% sensitivity when compared to the sensitivity obtained at its initial state, after continuously operating for 32 days. Undoubtedly, the sensitive, durable, miniaturized, and mass-produced pH sensor proposed in this article is a competitive device to implant into the water pipeline for pH in situ online monitoring.
研究领域:
生物医学工程
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微纳传感技术
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环境监测
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电化学传感器
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转化医学
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水质分析
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植入式器件
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DOI
高影响因子
SENSORS AND ACTUATORS A-PHYSICAL
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2025
·
IF 4.9
Conghai Ge,Yun Peng,Jiajia Shi,Yong Zhao
As an indispensable biomarker in hepatocellular carcinoma (HCC) screening, the highly sensitive detection of trace alpha-fetoprotein (AFP) provides a crucial auxiliary approach for the early screening and differential diagnosis of this malignant tumor. This paper presents a functionally integrated chimney-like microstructure for surface plasmon resonance (SPR) sensing. The proposed sensor is fabricated on the end-face of a single mode fiber (SMF) with an ultra-compact footprint of 10μm× 10μm× 16μm, achieving the advantages of miniaturization and trace liquid detection capability. The feasibility of the structure to excite SPR was verified through systematic simulations, and the critical geometric parameters were optimized. The structure features a facile fabrication process utilizing two-photon polymerization (2PP) technology combined with direct current (DC) diode sputtering. The proposed sensor demonstrated an AFP sensitivity of 0.684 nm/(ng/mL) with an ultra-low limit of detection (LOD) of 0.029 ng/mL, and exhibited excellent selectivity. Overall, this novel chimney-like SPR sensor achieves significant size reduction while maintaining exceptional AFP sensitivity compared to traditional sensors. These advances position the sensor as a promising platform for early-stage HCC screening and low-abundance biomarker analysis.
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高影响因子
MOLECULES
·
2020
·Vol. 26
·期 1
·38
·
IF 3.267
Yao Shen,Bo Chen,Han Zuilhof,Teris A. van Beek
Induced phase separation extraction (IPSE) is an efficient sample clean-up technique that can replace liquid-liquid extraction (LLE). The purpose of this study was to miniaturize IPSE by carrying it out in a microfluidic chip. An IPSE chip was designed and evaluated for its ability to separate and purify samples on a microscale. The 5 × 2 cm chip was fed with a solution of polar to non-polar model compounds in acetonitrile-water (1:1). In the 100 µm wide and 40 µm deep microchannels, the sample solution was efficiently separated into two immiscible phases by adding a hydrophobic solvent as inducer. Analytes present in the sample solution each migrated to their own favorable phase upon phase separation. After optimization, extraction and fractionation were easily and efficiently achieved. The behavior of analytes with a pH-dependent partitioning could be influenced by adjusting the pH of the sample solution.Scutellaria baicalensisextract, used in Traditional Chinese Medicine (TCM), was successfully separated in aglycones and glycosides. In this microscale system, the sample and solvent consumption is reduced to microliters, while the time needed for the sample pretreatment is less than one minute. Additionally, the extraction efficiency can reach up to 98.8%, and emulsion formation is avoided.Keywords:fast sample clean-up;IPSE;miniaturization;green analytical chemistry;μLPME;low solvent consumption;microfluidics;on-chip separation;TCM;Scutellaria baicalensis
研究领域:
分析化学
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中药学
·
微流控技术
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DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2021
·
IF 6.53
Danlan Fu,Junfei Huang,Kaitao Li,Yuxin Chen,Ye He,Yang Sun,Yilong Guo,Lijuan Du,Qian Qu,Yong Miao,Zhiqi Hu
Androgenic alopecia (AGA), also known as male pattern baldness, is one of the most common hair loss diseases worldwide. The main treatments of AGA include hair transplant surgery, oral medicines, and LDL laser irradiation, although no treatment to date can fully cure this disease. Animal models play important roles in the exploration of potential mechanisms of disease development and in assessing novel treatments. The present study describes androgen receptor (AR) in C57BL/6 mouse hair follicles that can be activated by dihydrotestosterone (DHT) and translocate to the nucleus. This led to the design of a mouse model of androgen-induced AGA in vivo and in vitro. DHT was found to induce early hair regression, hair miniaturization, hair density loss, and changes in hair morphology in male C57BL/6 mice. These effects of DHT could be partly reversed by the AR antagonist bicalutamide. DHT had similar effects in an ex vivo model of hair loss. Evaluation of histology, organ culture, and protein expression could explain the mechanism by which DHT delayed hair regrowth.
研究领域:
皮肤科
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药理学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2025
·
IF 2.7
Qiaolin Wang,Yulong Shi,Xiaojian Li,Junyao Zhang,Zefeng Hua,Xinyan Yang,Zhongfa Sun,Zhengbo Qin,Xianfeng Zheng
An integrated miniature time-of-flight mass spectrometer (TOF-MS) system coupled with a pocket-size 3D-printed laser-induced acoustic desorption (LIAD) source is described. This 3D-printed LIAD source utilizes only a miniature deceleration motor to achieve two-dimensional motion of the target surface, simplifying the source structure and improving the long-term stability of mass spectrometry measurements. It has been successfully applied to analyze the model molecule creatinine and ingredients in an energy beverage (Red Bull), where main natural nutrients were clearly identified. By adjusting the 3D printing parameters, the source can be readily adapted to various application scenarios requiring different sizes. This work provides a simple and efficient 3D-printed source injection strategy for subsequent investigations in molecular reaction dynamics.
研究领域:
分析化学
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生物医学工程
·
分子生物学
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代谢组学
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即时检测技术
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产品关联
DOI
PubMed
高影响因子
MATERIALS SCIENCE & ENGINEERING R-REPORTS
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2025
·
IF 26.8
Qihao Lv,Ruopeng Cui,Peihang Li,Xuefei Zhang,Yongjian Zhang,Chunlei Wan,Renchao Che,Yue Li
Pursuing extremely-thin, high-selectivity, and flexible absorbers is strongly required for mitigating the electromagnetic pollution in various miniaturized and space-limited scenarios, such as flexible electronic devices, cloaking systems, and anechoic chambers. Traditional synthetic methods, focusing on enhancing the real part of the absorbing materials’ permittivity through compositional and microstructural modifications, are however confronted with many limitations and have only reached centi-wavelength thick or even larger, impeding their integration into compact and flexible devices. Herein, we propose an interdisciplinary flexible metagraphene architecture, in which electromagnetic waves are selectively induced by the upper anomalous resonant-antiresonant metasurface into the lower ultrahigh-loss nitrogen-doped wavy graphene-based material for flexible, extremely-thin, and high-selectivity absorption. By overcoming the inherent physical contradiction of high loss and extremely-thin absorption, metagraphene achieves a perfect absorption with a top-class selectivity of 357.1 at a record-breaking thickness down to 0.001 λ 0 ( λ 0 represents the wavelength with the minimum reflection), surpassing state-of-the-art absorbers by 1–2 orders of magnitude. Furthermore, metagraphene offers further thinning potential, large-scale manufacturability, angular stability, and frequency universality, thus promising diversified applications in extremely-miniaturized electronic devices and space-constrained equipment.
研究领域:
电磁学
·
材料科学
·
纳米技术
·
微波工程
·
柔性电子学
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石墨烯技术
·
吸收体设计
·
超材料
·
电磁兼容
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DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 4
·047514
·
IF 4.386
Qiwen Yu,Jing Chen,Jingpin Xu,Long Chen,Yanhua Song,Xiaoya Xie,Jinjiang Jin,Hong Liu,Jun Liu,Fengjiang Zhang,Xing Chen
Real-time detection of L-lactate is crucial in monitoring tissue oxygenation and organ metabolism in surgery patients during the perioperative period. Traditional commercial L-lactate detection techniques using bulky and expensive instruments are time-consuming, hindering timely feedback in the operating room. Herein, utilizing the modified screen-printed carbon electrode, a portable and user-friendly smartphone-based electrochemical biosensing system, integrated with a miniaturized potentiostat via wireless transmission, was successfully constructed for bedside detection of L-lactate. It has a wide sensing range from 0.05–10 mM and a low limit of detection (9.1 μM). Bland–Altman analysis shows an acceptable agreement with the values of a mean difference (0.114 ± 0.3482) between results obtained with the commercial blood gas analysis instrument and the developed portable system. Accordingly, the developed portable system exhibits a great potential in point-of-care testing owing to its portability and rapid response in L-lactate detection during perioperative monitoring.
研究领域:
生物医学工程
·
分析化学
·
医疗器械
·
即时诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）