JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Shiqi Wang,Jiahui Wu,Qingcheng Wang,Yunfeng Zhang,Hong Yuan,Jianding Wang,Yonghui Wu,Yaxi Xu,Na Ji,Bin Quan,Haixing Wang,Qing Shen
A miniature mass spectrometer (mMS) based point-of-care testing (POCT) method was evaluated for on-site detecting the hypertension drugs, amlodipine and benazepril . The instrument parameters, including voltage, ISO1, ISO2, and CID , were optimized, under which the target compounds could be well detected in MS 2 . When these two drugs were injected simultaneously, the mutual ionization inhibition and mutual reduction between amlodipine and benazepril were evaluated. This phenomenon was severe on the precursor ions but had a small impact on the product ions, thus making this POCT method suitable for analysis using product ions. Finally, the method was validated and applied. The blood samples from patients were tested one hour after oral administration of the drugs (20 mg), and the benazepril was quantitatively analyzed using a standard curve, with detected concentrations ranging from 190.6 to 210 μg L −1 and a relative standard deviation (RSD) of 8.6 %. In summary, amlodipine has low sensitivity and can only be detected at higher concentrations, while benazepril has high sensitivity, good linearity, and even meets semi-quantitative requirements. The research results of this study are of great clinical significance for monitoring blood drug concentrations during hypertension medication, predicting drug efficacy , and customizing individualized medication plans.
研究领域:
分析化学
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临床医学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Yuanhao Zhou,Jiawen Ai,Lingli Cai,Yiyong Yan,BingZhi Wang,Hongzhen Ma,Quan Yu,Jianhua Zhou,Xinming Huo
Excessive pesticide use poses a significant threat to food safety. Rapid on-site detection of multi-target pesticide residues in vegetables is crucial due to their widespread distribution and limited shelf life. In this study, a rapid on-site screening method for pesticide residues on vegetable surfaces was developed by employing a miniature mass spectrometer . A direct pretreatment method involves placing vegetables and elution solution into a customized flexible ziplock bag, allowing thorough mixing, washing, and filtration. This process effectively removes pesticide residues from vegetable surfaces with minimal organic solvent usage and can be completed within 2 min. Moreover, this study introduced a deep learning algorithm based on a one-dimensional convolutional neural network, coupled with a feature database, to autonomously discriminate detection outcomes. By combining full scan MS and tandem MS analysis methods, the proposed method achieved a qualitative recognition accuracy of 99.62%. Following the qualitative discrimination stage, the target pesticide residue and internal standard can be simultaneously isolated and fragmented in the ion trap, thus enabling on-site quantitative analysis and warning. This method achieved a quantitative detection limit of 10 μg/kg for carbendazim in cowpea. These results demonstrate the feasibility of the proposed analytical system and strategy in food safety applications.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Ningxi Li,Zhijun Cai,Zhongyao Zhang,Wenpeng Zhang,Xiaoxiao Ma,Xiaoyu Zhou,Zheng Ouyang
In the past decade, mass spectrometry (MS) has been widely used for a broad range of on-site applications. This is largely attributed to the rapid advancement of technologies, such as ambient ionization and mass spectrometer miniaturization. Here, we report the development of the temperature-tuning desorption ionization (TTDI) method for versatile on-site applications using a miniature MS system. A unique feature of TTDI is its dynamic temperature range applicable from 30 to 800 °C, which enables optimal desorption ionization applied for chemical and biological compounds through tuning the temperature at the sampling spot. The versatility of TTDI has been demonstrated through on-site MS analysis of a variety of samples, such as explosives on surfaces, drugs of abuse in biofluids, and screening biomarkers in tissues.
研究领域:
分析化学
·
质谱技术
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Green Analytical Chemistry
·
2025
·
IF 6.2
Shuang Sun,Yijie Fu,Xinming Huo,Mi Luo,Quan Yu
Analysis of psychotropic drugs in blood is a suitable method to assess the effectiveness of patient treatment and the safety of drug dosage. Direct analysis of whole blood using mass spectrometry (MS) faces challenges due to the complex matrix and components in the sample. In this study, we developed a practical analytical strategy based on a miniature MS instrument for on-site detection of psychotropic drugs in blood. In particular, samples were first treated through a simplified and optimized extraction process and then analyzed by a miniature MS mounted with a capillary self-aspirating electrospray ionization source. This method reduced the consumption of samples and reagents while offering high accuracy and sensitivity, with a limit of detection as low as 0.1 ng/mL. We subsequently implemented the detection of venlafaxine and O-desmethyl venlafaxine in some whole blood samples. The results showed good agreement with those obtained by HPLC-MS, demonstrating the accuracy and practicality of the proposed analytical protocol.
研究领域:
分析化学
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生物医学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Linsen Li,Yulong Zeng,Ge Yang,Hao Liu,Chao Zhu,Ying Zhang,Feng Qu,Qiang Ma
Ambient ionization mass spectrometry (AIMS) allows rapid analysis of targets, while its overall selectivity is somewhat limited due to the lack of chromatographic separation. Recently, magnetic blade spray (MBS) has enhanced AIMS by incorporating immunomagnetic beads instead of the traditional coated blade spray (CBS) coating, thereby improving selectivity and sensitivity by targeted analyte detection and reducing background interference. In this study, an aptamer-functionalized and nucleic acid dye (GelRed)-loaded MS probe (AGMP) was developed and employed with MBS-based miniature mass spectrometer. Specifically, AGMP was assembled using aptamer-functionalized magnetic nanoparticles loaded with GelRed as mass tags for highly sensitive analysis of endoglin (CD105). For the preparation of AGMP, the CD105 binding aptamer of End-A2 was first selected through three rounds of capillary electrophoresis (CE)-SELEX with an optimal affinity of 62.3 pM. After optimizing the critical parameters that affected adsorption, desorption, and ionization efficiency, this method displayed satisfactory sensitivity with detection and quantitation limits of 0.2 and 1 ng/mL, respectively, as well as reliable recoveries of 90.1–106.8 % with relative standard deviations of 1.6–5.4 %. Besides, the method effectively mitigated the matrix effects with a slope deviation of 10.03 %, and exhibited good selectivity and environmental friendliness. Furthermore, this AGMP-based MBS strategy was successfully applied for CD105 detection in serum samples, demonstrating its potential for sensitive and on-site biomolecule analysis in complex matrices.
研究领域:
分析化学
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医学诊断
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生物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MASS SPECTROMETRY
·
2022
·Vol. 57
·期 3
·e4816
·
IF 2.394
Chengan Guo,Zhaoyin Diao,Jianli Liu,Baokun Yang,Jian Zhang
Miniature mass spectrometer is more compact and portable than traditional commercial mass spectrometry, with more potential for application outside the laboratory. However, a miniature mass spectrometer is less sensitive than a commercial instrument, limiting its application scenarios. The ion transmission efficiency of the instrument is an essential factor affecting the sensitivity. Still, there are few works of literature on the quantitative study of the ion transmission efficiency of each component from a systematic perspective. In this paper, the Faraday cup coupled with a microcurrent signal testing instrument was used to measure the ions generated by nanoelectrospray ionization (nano-ESI), which have successfully gone through several components. Then the ion transmission efficiency of each component was quantified. Results showed that the front lens had the highest ion transmission efficiency of 39.7%, whereas the inlet and skimmer had the lowest ion transfer efficiency of 0.8% and 17.1%. Next, the influence of control parameters on ion transmission efficiency of critical components was investigated. If optimized, the ion funnel and the skimmer had the potential to improve their transmission efficiency by 120% and 79%, respectively. This paper shows the decreasing intensity distribution of ions in the whole transmission process and the transmission efficiency of each component, which can guide for improving the sensitivity of the miniature mass spectrometer.
研究领域:
分析化学
·
质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Jingjin Fan,Penglong Lian,Ming Li,Xinwei Liu,Xiaoyu Zhou,Zheng Ouyang
Ion mobility (IM) has been increasingly used in combination with mass spectrometry (MS) for chemical and biological analysis. While implementation of IM with MS usually requires complex instrumentation with delicate controls, in this study we explored the potential of performing IM separation using dual-linear ion traps (LITs) in a miniature mass spectrometer, which was originally developed for performing comprehensive MS/MS scan functions with a simple instrumentation configuration. The IM separation was achieved by ion transfer between the LITs with dynamic gas flow. Its performance was characterized for analysis of a broad range of chemical and biological compounds including small organic compounds such as trisaccharides, raffinose, cellotriose, and melezitose, as well as protein conformers. The demonstrated technique serves as another example of developing powerful hybrid instrument functions with simple configurations and miniaturized sizes.
研究领域:
分析化学
·
质谱技术
·
生物化学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yanzuo Jiang,Di Huang,Hongjia Zhang,Ting Jiang,Wei Xu
Similar to smartphones, smart or automatic level is also a critical feature for a miniature mass spectrometer. Compared to large-scale instruments, miniature mass spectrometers often have a lower mass resolution and larger mass drift, making it challenging to identify molecules with close mass–charge ratios. In this work, a miniature mass spectrometer (the Brick-V model) was combined with intelligent algorithms to realize rapid and accurate identification. This Brick-V mass spectrometer developed in our lab was equipped with a vacuum ultraviolet photoionization (VUV-PI) source, which ionizes volatile organic compounds (VOCs) with minor fragments. Machine learning would be especially helpful when analyzing samples with multiple characteristic peaks. Four machine learning algorithms were tested and compared in terms of precision, recall, balanced F score (F1 score), and accuracy. After optimization, the multilayer perceptron (MLP) method was selected and first applied for the automatic identification and differentiation of ten different fruits. By recognizing the pattern of multiple VOCs diffused from fruits, an average accuracy of 97% was achieved. This system was further applied to determine the freshness of strawberries, and strawberry picking at different times (especially during the first 24 h at room temperature of winter) could be well discriminated. After building a database of 63 VOCs, a rapid method to identify compounds in the database was established. In this method, molecular ions, fragment ions, and dimer ions in the full mass spectrum were all utilized in the machine learning program. A satisfactory prediction accuracy for the 63 VOCs could be achieved (>99%).
研究领域:
分析化学
·
人工智能
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xinwei Liu,Xiao Wang,Jiexun Bu,Xiaoyu Zhou,Zheng Ouyang
Miniature mass spectrometers are of an increasing interest for in-situ analyses, and their coupling with the ambient ionization sources is a valid path for direct analysis of complex samples. In this study, a miniature mass spectrometer using discontinuous atmospheric pressure interface was developed with a dual-LIT (linear ion trap) configuration. The comprehensive scan modes were enabled for tandem mass spectrometry analysis, which are critical for high-quality qualitative and quantitative analysis. A real-time pressure control was implemented to facilitate the ion transfer and collision induced dissociation (CID). Beam-type CID could be performed for tandem analysis at a high number of stages. In-trap CID at high q could also be performed with the fragment ions accumulated in a second trap. A precursor ion scan mode for analyzing target analytes has also been demonstrated.
研究领域:
分析化学
·
质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Wenyan Shi,Xinming Huo,Xinyue Ding,Peiyuan Zhu,Yutong Wan,Xinqiong Lu,Rui Feng,Quan Yu,Xiaohao Wang
With the expansion of the functional food market, the qualification assessment of these products has become a major challenge, and efficient analytical tools are urgently needed. Here, a miniature mass spectrometer (MS) with self-aspiration capillary electrospray ionization (SACESI) source and ion trap analyzer was developed for rapid screening of various illegally added drugs in functional foods. No chromatographic separation was required, but a simplified two-step pretreatment method was developed to reduce the operational procedures and time consumption of the entire analysis. SACESI source uses capillary action to drive solution injection, which utilizes a simple structure and convenient operation to constitute a kind of disposable MS detection solution. To achieve accurate and automatic identification, an intelligent recognition algorithm with steps of spectrum preprocessing, characteristic peak matching, and support vector machine learning was constructed. The relative accuracy of rapid screening of 31 suspicious drugs in various samples is up to 99.78%. It achieves 100% correct identification for the 55 batches of actual samples captured by on-site inspection, which demonstrates the feasibility of the proposed analytical system and strategy in food safety applications.
研究领域:
食品安全
·
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yanping Zhu,Qian Zhang,Qian Zhang,Jun Lu,Kai Wang,Ruina Zhang,Quan Yu
With the advantages of rapid analysis, high sensitivity, and multicomponent identification, mass spectrometry (MS) is recognized as an appealing choice for high-throughput screening (HTS) analysis. Aiming at the small size, simple operation, and adequate performance, the development of miniature mass spectrometers has made great progress over the last 2 decades. Besides the essential analytical performance, simple operation and HTS capability are two other crucial features desired in miniature MS instruments. In this paper, an induced self-aspiration vacuum electrospray ionization source (ISA-VESI) was developed and coupled to a miniature ion trap mass spectrometer. A special timing sequence was designed to synchronize all the operation steps in each measurement, including dual-pulse sample injection, multipulse gas injection, MS analysis, and the movement of the homemade HTS platform used as the sampler. Then, the automatic high-throughput analysis of multiple samples can be accomplished with close coordination among the sample delivery, the sample introduction and ionization, and the ion trap operation. The measurement time of each ISA-VESI-MS analysis was about 7 s, with a sample consumption of less than 100 nL.
研究领域:
分析化学
·
质谱技术
✓ 已验证
DOI
PubMed
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2014
·Vol. 28
·期 15
·1764-1768
·
IF 2.642
Zhong-yao Zhang,Cuiping Li,Chuan-fan Ding,Fuxing Xu,Baoqiang Li,Qibin Huang,Hengxin Xia
RATIONALE The pivotal challenge associated with miniature mass analyzers is their proper design and construction without sacrificing performance. In order to analyze and improve the performance of a miniature linear ion trap with odd and even multipole fields, we designed a novel asymmetrical arc-shaped electrode ion trap (AAEIT), and tested the properties of AAEITs with different dimensions. METHODS A series of asymmetrical ion traps using arc-shaped electrodes were designed to optimize the properties (resolutions and intensity) of the coupling effects between odd and even multipole fields. Using arginine and reserpine, we evaluated the performance of mass resolution, ion intensity ratio and deduced the collision-induced dissociation (CID) efficiency using a self-constructed electrospray ionization mass spectrometry (ESI-MS) platform. RESULTS An AAEIT with field radius dimensions of 5 mm × 5.75 mm exhibits a good performance: its maximum resolution of 833 (FWHM) at m/z 175 was achieved for the side of small electrode. With this AAEIT, a tandem mass (MS/MS) capability with 91.0% CID efficiency was obtained with reserpine ( m/z 609). CONCLUSIONS The results indicated that the AAEIT, comprising both odd and even multipole fields, could act as a qualified linear ion trap mass analyzer with compact structure, high resolution, and high tandem mass analysis efficiency. It has a great potential in miniature mass spectrometry. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）