ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xun Liu,Shang-Qing Zhang,Zi-Han Cheng,Xing Wei,Ting Yang,Yong-Liang Yu,Ming-Li Chen,Jian-Hua Wang
A highly sensitive platform is developed for the determination of microRNA-21 (miRNA-21) with inductively coupled plasma mass spectrometry (ICPMS). It includes the following operations: Hairpin structures DNA H1 and H2 are designed, and DNA H1 is bound to ultrasmall lanthanide upconversion nanoparticles (UCNPs) to produce UCNPs@DNA conjugate probes. Target miRNA triggers a chain reaction for alternating hybridization between DNA H1 (bound on UCNPs@DNA probe) and DNA H2. This leads to UCNPs accumulation and serves as an efficient amplification strategy for UCNPs. The concentration of miRNA-21 is closely correlated to the number of UCNPs; thus, the detection of 89Y by ICPMS provides a promising approach for miRNA quantification. This protocol exhibits high sensitivity to miRNA-21 within 0.1–500 fM, along with a detection limit of 41 aM, which is among the hitherto reported most sensitive procedures. It is worth mentioning that rare earth elements are scarcely present in living systems, which minimizes the background for ICPMS detection and excludes potential interferences from the coexisting species, which is most suited for biological assay.
研究领域:
生物化学
·
分析化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
COMPOSITES COMMUNICATIONS
·
2024
·
IF 6.5
Minghui Xu,Xiaoru Yao,Yichen Wang,Haihan Liu,Wenjia Yang,Pingping Zhang
In this paper, core-sheath nanofiber membrane (miRNA-21@DCH) co-loaded with microRNA-21 and doxycycline (DCH) was prepared by coaxial electrospinning technology for the treatment of diabetic wounds. DCH could inhibit the growth of Gram-negative and Gram-positive bacteria that easily cause infection at the wound site, thus promoting the epidermal layer thickening and dermal connective tissue hyperplasia. The cumulative release rate of DCH was 89 % after 72 h. In vitro antibacterial experiments at 24 h showed that the bacteriostatic zone diameter of miRNA-21@DCH nanofiber membrane was about 3.82 cm for Escherichia coli and 4.54 cm for Staphylococcus aureus , indicating that miRNA-21@DCH nanofiber membrane had good antibacterial effect. The microRNA-21 could promote angiogenesis and collagen deposition at the wound site, providing a favorable growth environment for tissue cell proliferation. The synergistic effect of DCH and microRNA-21 confers miRNA-21@DCH core-sheath nanofiber membrane the advantage of rapid healing of diabetic wounds. After 16 days treatment with miRNA-21@DCH nanofiber membrane, the wound basically healed and the wound surface was smooth. In addition, in vitro cytotoxicity assays showed that miRNA-21@DCH nanofiber membrane had good biocompatibility. The prepared miRNA-21@DCH nanofiber membrane provides a new and effective strategy for the synergistic treatment of diabetic wounds.
研究领域:
生物材料学
·
药理学
·
再生医学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Cheng Yuan,Qiqi Wu,Ke-Xin Xu,Xing-Shi Liu,Hao Lou,Yi-Tong Xu,Zheng Li,Yuanyuan Meng,Tan Li,Rui Ban,Guangxu Chen,Wei-Wei Zhao
The field of organic photoelectrochemical transistor (OPECT) is newly emerged, with increasing efforts attempting to utilize its properties in biological sensing. Advanced materials with new physicochemical properties have proven important to this end. Herein, we report a metal-organic polymers-gated OPECT biosensing exemplified by Cu Ⅰ -arylacetylide polymers (CuAs)-modulated poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel. Both the photoelectrochemical properties and gating capability of CuAs are explored and optimized for high-efficacy photogating. Morever, based on its inherent structure, the specific reaction between CuAs and sulfur ions (S 2− ) is revealed and S 2− -mediated microRNA-21 detection is realized by linking with nucleic acid amplification and alkaline phosphatase catalytic chemistry. This work introduces metal-organic polymers as gating materials for OPECT biosensing.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wu Chen,Mengfan Guo,Zeyu Chen,Ying Sun,Yudian Huang,Yushan Chen,Yanyan Ma,Juan Sun,Bin Qiu,Hui Lin
The overexpression of microRNA-21 (miRNA-21) is closely associated with gastric cancer (GC) and may be used as a biomarker to diagnose GC early. Here, a colorimetry and fluorescence dual-mode biosensor for the detection of miRNA-21 was developed from polydopamine-coated magnetic upconversion nanoparticles (Fe 3 O 4 @UCNP@PDA) and aptamer-modified gold nanoparticles (AuNP-apt). The strong adsorption of single-stranded DNA by PDA results in close proximity between the energy donors (UCNPs) and acceptors (AuNPs), resulting in luminescence quenching of the UCNPs. The miRNA-21 can hybridize with the apt-conjugated AuNPs, and the AuNPs move away from the UCNPs, which results in the luminescence recovery of the UCNPs and the catalytic activity of the AuNPs. After H 2 O 2 and tetrapaminophenylethylene were added, the yellow solution turned green and gradually deepened with increasing miRNA-21 concentration. Under optimal conditions, the detection limits of miRNA-21 by fluorescence and colorimetry assays are 8.90 pM and 185 pM, respectively. In addition, the developed approach was utilized to measure miRNA-21 in serum samples, and the recovery rates ranged from 105.4-107.1 % and 98.5–109.3 %, respectively. The above results indicate great promise for the early diagnosis of GC because of its high sensitivity and selectivity.
研究领域:
生物化学
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分子诊断
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Po Li,Xueying Lei,Xiaoying Niu,Wen Tian,Zhehuang Li,Songcheng Yu,Peng Zhang
MicroRNA (miRNA) is promising candidate for non-invasive diagnostic biomarker. Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription, nucleic acid pre-amplification and costly fluorescently labeled reporters which introduce chemical modification complexity and background noise. To address these limitations, we herein developed a multifunctional switch that integrated target recognition, CRISPR/Cas12a system activation, intrinsic fluorescence signaling, and autonomous signal amplification within a single molecular architecture. As a proof of concept, this switch enabled a label-free CRISPR/Cas12a biosensing for miR-21 detection with a detection limit of 4.8 nM and robust performance in accuracy, precision, and selectivity. This proposed label-free CRISPR/Cas12a platform could be applied for real sample and is a promising candidate for point-of-care miRNA detection.
研究领域:
生物传感技术
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分子诊断
·
CRISPR 基因检测
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核酸扩增
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即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2021
·
IF 5.373
Ya Zhang,Ning Li,Wenhao Ma,Mei Yang,Changjun Hou,Xiaogang Luo,Danqun Huo
MicroRNA exhibits different levels of expression in cancer and can affect the transformation, metastasis, and carcinogenesis of the cancer cell. Herein, we developed a novel kind of electrochemical microRNA biosensor based on two-dimensional nanomaterial of antimonene nano-flakes (AMNFs) and carbon quantum dots (CQDs) which were used as substrating to cadmium ion (Cd 2+ ) for specific detection of breast cancer-relevant biomarker-microRNA-21. Compared to graphene, the first principle energetic calculation shows that the AMNFs have completely a stronger force interaction with single strand (ssRNA), due to the antimonene has a more delocalized 5 s/5p orbital. After the addition of complementary microRNA, due to the low adsorption affinity of double-stranded RNA (dsRNA) to antimonene, the hybridized target is easy to desorb from the antimonene interface, and the oxidation peak of metal ions is significantly reduced. Results showed the microRNA-21 concentration can be detected from 100 aM to 1 nM, the limit of detection as low as 21 aM toward microRNA-21, which is 3 times lower than those of the established microRNA biosensors. The unique combination of not be attempted before existing sensing material which has special adsorption properties represents an approach to the detection of breast cancer. And it provides a promising method for early diagnosis, monitoring, and staging of breast cancer.
研究领域:
生物化学
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纳米技术
·
癌症研究
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
·
2020
·
IF 2.014
Li YAO,Yu-Xia YANG,Hui CAO,Huan-Huan REN,Zheng NIU,Ling SHI
Osthole is observed to have the capacity to treat pulmonary arterial hypertension (PAH) in rats, but molecular mechanism is still unknown. The present study aims to discover therapeutic targets and explore therapeutic mechanism of osthole against PAH from metabolic perspective. A rat model with PAH was successfully established with MCT, following osthole administration, then untargeted metabolomics assay was performed using UPLC-Q-TOF-MS to identify differential metabolites and associated metabolic pathways, at last mechanism investigation was done by qRT-PCR, Western blot and ELISA. Differential metabolites characterized in rats with PAH were mostly assigned to sphingolipid metabolism, synthesis of unsaturated fatty acids, glycolysis, nucleotide metabolism, steroid hormone biosynthesis. Furthermore, osthole reversed high level of S1P by modulating metabolic enzyme Sphk1 in rats with PAH. In addition, osthole inhibited the expression of Sphk1 by downregulating microRNA-21, phosphorylation of Akt, phosphorylation of mTOR in vivo and in vitro . These results demonstrated that metabolomics is a promising approach to discover potential drug target for PAH treatment. Importantly, our findings further elucidated therapeutic mechanism of osthole, a natural product, having a role of metabolic regulator to potentially treat PAH by targeting inhibition of Sphk1/S1P via microRNA-21-PI3K/Akt/mTOR signal pathway. Altogether, this discovery paves a critical foundation for enabling osthole to be a candidate compound to treat PAH.
研究领域:
药理学
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心血管疾病
·
代谢紊乱
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Fu-Ting Wang,Jing Xu,Yang-Yang Hou,Ke-Jing Huang,Xin-Meng Yu,Xin Zhou,Xuecai Tan
A capacitor coupled with enzymatic biofuel cells (EBFCs) can deliver high-power instant output to obtain an amplified detection signal. Herein, matching a capacitor to a self-powered electrochemical biosensor for ultrasensitive determination of microRNA-21 is investigated. The detection system mainly combines a capacitor, EBFCs, and biological amplification technologies. Concretely, EBFCs are integrated into a capacitor-joined circuit, and the capacitor is automatically shorted by a switching regulator to provide an instantaneous current that is rapidly detected with a digital multimeter. A sensitivity of 38.72 μA/pM is achieved with the contribution of the matching capacitor, which is 6.96 times that without a capacitor. Moreover, the redox reaction on the anode leads to high-voltage outputs at low substrate concentrations and generates electrons when the target miRNA triggers a catalytic hairpin assembly cycle, while the release of the [Fe(CN)6]3– electron acceptor on the cathode is catalyzed to obtain a higher detection signal. As a result, the limit of detection of the developed biosensor is 0.18 fM (S/N = 3) with a linear range of 0.5–10 000 fM, which indicates that the innovative capacitor-matched self-powered biosensor holds great promise for ultrasensitive electrochemical biosensing.
研究领域:
生物化学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Keying Zhang,Na Zhang,Yilin Wu,Wen Zhang,Fajun Li,Yizhong Shen
MicroRNA (miRNA) upconversion nanoprobes using organic dyes as acceptors have attracted intensive attention in early cancer diagnosis, but still remains a large challenge from the unsatisfactory luminescence resonance energy transfer (LRET) efficiency. Herein, we proposed to integrate the confined emitters effect and target cycling amplification strategy into a single matrix for establishing the upconversion nanoamplicon with high LRET efficiency, allowing for accurately reporting the levels of miRNA originated from cancer cells. As a proof-of-concept, the emitting ions were confined in the shell of the core-shell upconversion nanoparticles (UCNPs), enabling an enough close distance to its surface energy acceptors. The tentacle DNAs (P-tDNA-M), phosphorylated and methylated at both ends, were immobilized onto the UCNPs surfaces for effectively capturing hairpin DNA1 labeled with BHQ1 (BHQ1-HP1) to form BHQ1-UCNPs energy donor-acceptor pair, achieving ∼ 67.9 % of LRET efficiency. Target miRNA-21 could bind with HP1 to open the loop, causing BHQ1 away from UCNPs, thereby restoring the upconversion luminescence, allowing for high sensitively detecting miRNA-21 with a detection limit of 75.3 fM. Interestingly, this naoprobe exhibited good performances for monitoring miRNA-21 in human serum samples and evaluating the expression level of miRNA-21 in various cell lines. This proposed platform gives a new insight to design the efficient upconversion nanoprobes for precisely detecting low-expressed miRNA in complex biological samples.
研究领域:
生物医学工程
·
分子诊断学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Huiling Fan,Yuqi Qin,Long Li,Jiansheng Cui,Chenqiang Yang,Zhanhui Wang,Lili Duan,Yihong Wang,Liang Tian
Herein, a biosensing system was designed based on tripedal DNAzyme walker integrated nanoprobe signal amplification strategy for realizing ultrasensitive determination of the microRNA-21. In this work, the two-dimensional MoSe 2 /MXene nanocomposite with high electronic conductivity was synthesized as electrode signal amplification materials to immobilize more capture DNA and accelerate electron transport. After adding microRNA-21, the tripedal DNAzyme walker was activated on the surface of the Pt@CeO 2 nanoclusters, which were as nanozyme to construct the high-performance nanoprobe machine. Then, the tripedal DNAzyme strands could cleave the substrate strands and walk onto the nanoprobe for amplifying the signal remarkably. Furthermore, the detectability of the electrochemical biosensor was verified by DPV, acquiring a wide linear response in a range of 0.1 fM to 1 nM, as well as the limit of detection was 0.04 fM. Hence, such double signal amplified strategy could not only provide excellent selectivity and specificity, but also achieve superior repeatability and stability in real complex serum samples. Therefore, the designed biosensing platform detected different microRNA through modulating the recognition sequence, which might provide a general strategy for various cancer analyses and have a promising potential for clinical application.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Ting Bao,Ruobing Fu,Yuying Jiang,Wei Wen,Xiuhua Zhang,Shengfu Wang
The development of a robust sensing platform with an efficient probe assembly, and ingenious signal conversion is of great significance for bioanalytical application. In this work, a multipedal polydopamine nanoparticles–DNA (PDANs-DNA) nanomachine coupling electrochemical-driven metal–organic frameworks (MOFs) conversion-enabled biosensing platform was constructed. The PDANs-DNA nanomachine was designed based on Ca2+-mediated DNA adsorption and target-triggered catalytic hairpin assembly on PDANs, which not only maintained the DNA immobilization simplicity but also possessed a high walking efficiency. PDANs-DNA nanomachine could walk fast on the electrode via multiple legs under exonuclease III driving, resulting in the formation of DNA dendrimers through two hairpins assembly. The MOFs (Fe-MIL-88-NH2) probe was decorated on the DNA dendrimers to act as a porous metal precursor and converted into electroactive Prussian Blue by a controlled electrochemical approach, which was a facile, simple, and room-temperature approach compared with the commonly employed MOFs conversion methods. Using microRNA-21 (miRNA-21) as the model target, the proposed biosensor achieved miRNA-21 detection ranging from 10 aM to 10 pM with the detection limit of 5.8 aM. The proposed strategy presented a highly efficient walking platform with the ingenious electrochemical conversion of MOFs, providing more options for the design of an electrochemical platform and holding potential applications in clinical analysis and disease diagnosis.
研究领域:
生物分析化学
·
纳米生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 11
·1-11
·
IF 5.833
Wei Jinlan,He Sitian,Mao Yanhua,Wu Longjie,Liu Xinlian,Effah Clement Yaw,Guo Hongchao,Wu Yongjun
A simple nanoplatform based on molybdenum disulfide (MoS 2 ) nanosheets, a fluorescence quencher (signal off), and a hybridization chain reaction (HCR) signal amplification (signal on) used for the enzyme-free, label-free, and low-background signal quantification of microRNA-21 in plasma exosome is reported. According to the sequence of microRNA-21, carboxy-fluorescein (FAM)-labeled hybridization probe 1 (FAM-H1) and hybridization probes 2 (FAM-H2) were designed with excitation maxima at 488 nm and emission maxima at 518 nm. MoS 2 nanosheets could adsorb FAM-H1 and FAM-H2 and quenched their fluorescence signals to reduce the background signal. However, HCR was triggered when microRNA-21 was present. Consequently, HCR products containing a large number of FAM fluorophores can emit a strong fluorescence at 518 nm and could realize the detection of microRNA-21 as low as 6 pmol/L and had a wide linear relation of 0.01–25 nmol/L. This assay has the ability of single-base mismatch recognition and could identify microRNA-21 with high specificity. Most importantly, this approach was successfully applied to the detection of plasma exosomal microRNA-21 in patients with lung cancer, and it is proposed that other targets can also be detected by changing the FAM-H1 and FAM-H2 corresponding to the target sequence. Thus, a novel, hands-on strategy for liquid biopsy was proposed and has a potential application value in the early diagnosis of lung cancer. Graphical abstract
研究领域:
癌症诊断
·
分子生物学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）