REGULATORY TOXICOLOGY AND PHARMACOLOGY
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2015
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IF 2.031
Chenzhong Fei,Jie Zhang,Yang Lin,Xiaoyang Wang,Keyu Zhang,Lifang Zhang,Wenli Zheng,Mi Wang,Tao Li,Sui Xiao,Feiqun Xue,Chunmei Wang
Nitromezuril (NZL) is a novel triazine compound that exhibits remarkable anticoccidial activity. However, mutagenicity and genotoxicity of NZL have not been evaluated to date. This study evaluated the potential risks of NZL by testing for bacterial reverse mutation (Ames), mouse sperm abnormality (SA), bone marrow micronucleus (MN) and chromosomal aberration (CA). Mice were orally administered with NZL at 385, 192 and 96 mg/kg, corresponding to 0.5×, 0.25× and 0.125× the LD 50 of NZL, respectively. No significant increases in SA and CA were found in mice treated with NZL for 5 d and 3 d, respectively ( P > 0.05). NZL at 96–385 mg/kg did not have significant influence on micronucleated polychromatic erythrocyte counts ( P > 0.05). These results suggest that NZL is not genotoxic. However, Ames test results were positive both with and without the S9 system for Salmonella typhimurium TA98 and TA100, suggesting that NZL may be mutagenic. The mutagenic effects of NZL were different in in vitro and in vivo assays . Further studies should be conducted to confirm the safety of using and developing NZL as a novel anticoccidial drug.
研究领域:
毒理学
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遗传学
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产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
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2013
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IF 3.01
Jianyong Li,Xiaojun Kong,Xiwang Li,Yajun Yang,Jiyu Zhang
Aspirin eugenol ester (AEE) is a promising drug candidate for treatment of inflammation, pain and fever and prevention of cardiovascular diseases with less side effects and it is important to characterize its genotoxicity. In this study, the genotoxicity of AEE was assessed with two standard genotoxicity assays of the Salmonella typhimurium mutagenicity assay (Ames test) and the mouse bone marrow micronucleus assay. In the Ames test , Salmonella strains TA97, TA98, TA100, TA102 and TA1535 were treated with or without the metabolic activation with a S9 fraction from Acroclor-induced rat liver. The doses of AEE were 5 mg/plate, 2.5 mg/plate, 1.25 mg/plate, 0.625 mg/plate and 0.3125 mg/plate, respectively. In the above tested strains, mutagenicity with or without the S-9 mixture was not detected. In the mammalian erythrocyte micronucleus assay, fifty mice were divided into five groups evenly and the AEE dose at 5000 mg/kg, 2500 mg/kg and 1250 mg/kg and the cyclophosphamide dose at 40 mg/kg as a positive control, the 0.5% of CMC-Na as negative control were administered. The results showed that AEE did not induce any significant increase in micronucleated erythrocytes after 24 h ( p < 0.01). Our results suggested that AEE was non-genotoxic in vivo or in vitro .
研究领域:
药理学
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毒理学
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遗传学
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产品关联
DOI
PubMed
高影响因子
Foods
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2024
·Vol. 13
·期 9
·1404
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IF 4.7
Yuyang Yao,Juer Liu,Qiming Miao,Xinyue Zhu,Wei Hua,Na Zhang,Guangwei Huang,Xiangyang Lin,Shengquan Mi,Yanling Cheng,Roger Ruan
Almond hull, a substantial byproduct comprising more than half of almond fresh weight, has recently gained attention due to its functionality and sustainability benefits. Despite heightened interest, information regarding its toxicity remains limited. In order to assess its genotoxic potential, we conducted Good Laboratory Practice-compliant in vitro and in vivo studies following Organization for Economic Co-operation and Development (OECD) guidelines. No evidence of toxicity or mutagenicity was observed in a bacterial reverse mutation assay using five tester strains, evaluating almond hull at concentrations up to 5 mg/plate, with or without metabolic activation. Almond hull did not induce chromosome structural damage in a chromosome aberration assay using Chinese hamster ovary cells, nor did it cause any spermatogonial chromosomal aberration in tested male BALB/c mice. To evaluate its ability to induce DNA damage in rodents, a combined micronucleus assay was conducted in KM mice of both sexes. Almond hull was administered at doses of 1250, 2500, and 5000 mg/kg/day via gavage once daily for 2 days. No adverse effects of almond hull were observed in the micronucleus assay. Our results indicate no evidence of the genotoxic potential of almond hull administered up to the maximum concentrations of 5 g/kg, as recommended by OECD guidelines.
研究领域:
食品科学
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毒理学
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遗传学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
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2021
·Vol. 40
·期 7
·1917-1925
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IF 3.742
Hongyi Xian,Meilin Tang,Ying Chen,Zhenwei Lian,Yanfeng Li,Xiaowu Peng,Dalin Hu
The present study was conducted to assess the genotoxic potential of water from the Helong Reservoir, which was designated as a strategic drinking water source by the Guangdong Provincial Government of China in October 2016. Four kinds of common indigenous fish samples ( Labeo rohita , Cirrhinus molitorella , red tilapia, and Oreochromis niloticus ) were collected at 6 sampling sites during the period from July to November 2020. Fish from the clean drinking water source of the upper reaches of the Liuxihe Reservoir in Guangzhou were collected as the control. Both the alkaline single cell gel electrophoresis assay and the micronucleus test were used to detect DNA damage and the micronucleus rate in erythrocytes of fish samples, respectively. The results indicated that there was a significant increase in comet tail length, Olive tail moment, and micronucleus rates of all fish samples compared with those of the control ( p < 0.05). The order of sensitivity to DNA damage and micronucleus formation was Labeo rohita > Cirrhinus molitorella > red tilapia > Oreochromis niloticus . The results of the 2 kinds of experiments were in perfect agreement with each other. We conclude that there are obvious genotoxic effects from the water in the Helong Reservoir. As a strategic drinking water source, the safety of the Reservoir water quality should be considered. The local government should put the restoration of the Helong Reservoir water quality on the agenda as soon as possible. Environ Toxicol Chem 2021;40:1917–1925. © 2021 SETAC
研究领域:
环境科学
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毒理学
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水生生物学
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水质管理
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DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
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2013
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IF 3.01
Hui Dong,Demei Xu,Lihua Hu,Lingrui Li,Erqun Song,Yang Song
Tetrachlorobenzoquinone (TCBQ) is an active metabolite of pentachlorophenol (PCP). Although the genotoxic effect of PCP has been comprehensively investigated, there is little known about TCBQ’s genotoxic effects. In the current study, TCBQ was tested for its genotoxicity using HepG2 cells as experimental model. To select the exposure concentration of interest, cell viability was measured and three concentrations were used for further investigation. In single cell gel electrophoresis (SCGE) assay, concentration-dependent increase in tail length, tail DNA percentage and tail moment were detected following TCBQ exposure. Micronucleus (MN) assay indicated TCBQ gradually increased MN frequency and decreased nuclear division index (NDI). Enzyme-linked immunosorbent assay (ELISA) and western blotting analyses both showed TCBQ caused histone H2AX phosphorylation (γ-H2AX). Furthermore, the elevation of 8-hydroxydeoxyguanosine (8-OHdG) and reactive oxygen species (ROS) level indicated TCBQ-induced genotoxicity is associated with oxidative stress. On the other hand, N-acetyl-cysteine (NAC) administration significantly protected cells from the genotoxic effect of TCBQ. Overall, our data suggested TCBQ exerted genotoxic effect possibly via an oxidative damage mechanism in HepG2 cells and this toxicity is prevented by pretreatment with NAC.
研究领域:
毒理学
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细胞生物学
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遗传学
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DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
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2014
·
IF 3.355
Zhangjian Chen,Yun Wang,Te Ba,Yang Li,Ji Pu,Tian Chen,Yanshuang Song,Yongen Gu,Qin Qian,Jinglin Yang,Guang Jia
With the extensive application of titanium dioxide (TiO 2 ) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO 2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO 2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO 2 NPs (75 ± 15 nm) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days. The γ-H 2 AX assay showed TiO 2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO 2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO 2 NPs at the dose of 0, 5, 10, 20, 50 and 100 μg/mL. Significant decreases in cell viability were detected in all the treated groups after 24 h and 48 h exposure. Significant DNA damage was only observed at the concentration of 100 μg/mL after 24 h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 μg/mL after 2 h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO 2 NPs, and TiO 2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO 2 NPs induced genotoxic effects.
研究领域:
毒理学
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2021
·
IF 7.086
Jingyi Fu,Li Feng,Yongze Liu,Liqiu Zhang,Shuo Li
Electrochemical activation of peroxymonosulfate (PMS) at carbon cloth anode (E (Carbon cloth Anode)/PMS system) was investigated for sulfamethoxazole (SMX) degradation. The results indicated that PMS could be activated at carbon cloth anode during electrolysis, resulting in the improvement of SMX degradation. The degradation efficiency of SMX was facilitated with the higher PMS concentration and current density, respectively. The degradation rate constant of SMX increased with the rising pH from 3.6 to 6.0, and reached the highest value at pH 6.0, and then decreased with further increasing pH to 8.0. The presence of chloride ion (Cl − , 5–100 mM) significantly enhanced SMX degradation, while addition of humic acid (HA, 1–5 mgC L −1 ) inhibited SMX degradation. Addition of carbonate (HCO 3 − , 5–20 mM) had a negligible impact on SMX degradation. Small amounts of phosphate (PO 4 3− , 0–5 mM) could promote degradation, while a large amount of PO 4 3− (10–20 mM) inhibited the degradation. Moreover, the quenching experiments demonstrated that sulfate radical (SO 4 · − ), hydroxyl radical (·OH) and singlet oxygen ( 1 O 2 ) contributed to SMX degradation in E (Carbon cloth Anode)/PMS system. The degradation intermediates of SMX were identified by LC-MS/MS and the degradation pathways were deduced to be hydroxylation, the cleavage of S–N bond, and oxidation of aniline group. Moreover, the micronucleus test of Vicia faba root tips indicated that the E (Carbon Cloth Anode)/PMS system could reduce the genetic toxicity of SMX contaminated water to some extent.
研究领域:
环境化学
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电化学
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水处理
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毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）