JOURNAL OF HAZARDOUS MATERIALS
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2019
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IF 7.65
Haiyun Li,Yizhi Qiu,Tuo Yao,Yachun Ma,Huirong Zhang,Xiaolei Yang,Changning Li
Environmental problems caused by the large-scale use of chemical pesticides are becoming more and more serious, and the removal of chemical pesticides from the ecological environment by microbial degradation has attracted wide attention. In this study, using enrichment screening with seven chemical pesticides as the sole carbon source, a mixed microbial culture (PCS-1) was obtained from the continuous cropping of strawberry fields. The microbial community composition, degradation ability, and detoxification effect of PCS-1 was determined for the seven pesticides. Inoculation with PCS-1 showed significant degradation of and tolerance to the seven pesticides. Microbial community composition analysis indicated that Pseudomonas , Enterobacter , Aspergillus , and Rhodotorula were the dominant genera for the degradation of the seven pesticides by PCS-1. The concentration of the seven pesticides was 10 mg L −1 in hydroponic and soil culture experiments. The fresh weight, plant height, and root length of PCS-1-inoculated alfalfa ( Medicago sativa ) significantly increased compared with those of non-PCS-1-inoculated M. sativa . PCS-1 not only effectively degraded the residual content of the seven pesticides in water and soil but also reduced the pesticide residues in the roots, stems, and leaves of M. sativa . This study shows that PCS-1 may be important in environmental remediation involving the seven pesticides.
研究领域:
环境科学
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微生物学
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农业
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 11
·4388-4402
·
IF 3.9
Liu Wu,Linyu Zhao,Yingmei Tao,Di Zhang,An He,Xiaozhong Ma,Huan Zhang,Guoliang Li,Liangyan Rong,Ruren Li
Commercial starter cultures play a critical role in the industrial production of fermented sausages. However, commercial starter cultures could not reproduce the metabolic actions of diverse microorganisms and the aroma profile of the traditional spontaneously fermented sausages. Identifying the core microbial community in spontaneously fermented sausages will facilitate the construction of a synthetic microbial community for reproducing metabolic actions and flavor compounds in spontaneously fermented sausages. This study aimed to reveal the core microbial community of spontaneously fermented sausages based on their relative abundance, flavor-producing ability, and co-occurrence performance. We identified five promising genera to construct the synthetic core microbial community, these were Lactobacillus , Staphylococcus , Macrococcus , Streptococcus , and Pediococcus . Sausages inoculated with a synthetic core microbial community presented higher quality of aroma profile than the fermented sausages inoculated with a commercial starter culture. Some important volatile flavor compounds of spontaneously fermented sausage, such as (-)- β -pinene, β -caryophyllene, 3-methyl-1-butanol, α-terpineol, ethyl 2-methylpropanoate, and ethyl 3-methylbutanoate which are associated with floral, fruity, sweet, and fresh aromas, were also detected in fermented sausage inoculated with synthetic microbial community. This indicated that the synthetic core microbial community efficiently reproduced flavor metabolism. Overall, this study provides a practical strategy to design a synthetic microbial community applicable to different scientific fields.
研究领域:
食品科学
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微生物学
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发酵技术
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DOI
PubMed
高影响因子
Processes
·
2019
·Vol. 7
·期 9
·590
·
IF 1.963
Tingting Zhong,Yali Liu,Xiaorong Kang
Batch tests were carried out to study the effect of simultaneous addition of ethylenediaminetetraacetic acid and Ni2+(EDTA-Ni) on anaerobic digestion (AD) performances of kitchen wastes (KWs). The results indicated that the cumulative biogas yield and methane content were enhanced to 563.82 mL/gVS and 63.7% by adding EDTA-Ni, respectively, which were almost 1.15 and 1.07-fold of that in the R2 with Ni2+addition alone. At the same time, an obvious decrease of propionic acid was observed after EDTA-Ni addition. The speciation analysis of Ni showed that the percentages of water-soluble and exchangeable Ni were increased to 38.8% and 36.3% due to EDTA-Ni addition, respectively. Also, the high-throughput sequencing analysis revealed that the EDTA-Ni promoted the growth and metabolism ofMethanosarcinaandMethanobacterium, which might be the major reason for propionic acid degradation and methane production.Keywords:EDTA;kitchen waste;methane;microbial community;Ni2+
研究领域:
环境科学
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微生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2025
·
IF 5.2
Xianhao Zhang,Bingrui Guo,Bing Dai,Shimiao Wang,Siqi Zhang,Sufang Zhang,Chaofan Ji,Beiwei Zhu,Xinping Lin
In this study, the effect of fermentation on the safety, flavor, and texture of largemouth bass (Micropterus salmoides ) was investigated. Compared to fresh fish, the proteins formed a network structure that enhanced bound water proportion, texture hardness, and water holding capacity, while it reduced cooking losses. TVB-N and TBARS levels increased gradually but stayed within safe limits. However, histamine accumulation surpassed the recommended limit at 56.84 ± 0.17 mg/100 g in late stages. A total of 67 volatile compounds were detected, with 13 identified as key flavor compounds (OAV > 1). 3rd-generation full-length 16S rRNA gene sequencing revealed a microbial community shift from high diversity to dominance by strains like Vibrio anguillarum , Vagococcus fessus , and Vagococcus coleopterorum . Among these, 10 species were highly correlated with flavor formation. This study reveals quality patterns in fermented largemouth bass, providing insights into achieving a balance among safety, flavor, and texture in fermented fish.
研究领域:
食品科学
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微生物学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2011
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IF 3.723
Xinying Zhang,Xiaoyan Liu,Shanshan Liu,Fahui Liu,Lisha Chen,Gang Xu,Chenglin Zhong,Pengcheng Su,Zhengnan Cao
The aim of this study was to determine the enhancement of Scirpus triqueter in the dissipation of pyrene and the interaction of pyrene with plant, soil enzymes and microbial community. The results indicated that the dissipation ratios of pyrene in the rhizospheric and non-rhizospheric soil were 64.65 ± 3.86% and 54.49 ± 2.74%, respectively, and were higher than that in the unplanted soil (42.60 ± 0.71%) at 80d after planting S. triqueter . The pyrene was toxic to S. triqueter , as evidenced by growth inhibition in height, diameter, shoot number and biomass during the planting period. The activities of dehydrogenase decreased significantly at the presence of pyrene in soils, and increased remarkably with the introduction of S. triqueter . It was found that the pyrene addition increased the ratios of fungal/total fatty acids and gram-positive/gram-negative, but the presence of S. triqueter decreased the ratios of gram-positive/gram-negative. A larger stress level was found in the pyrene treated soils without S. triqueter . The ratio of aerobic/anaerobic bacteria decreased with increasing pyrene concentration, but increased when S. triqueter was planted. The principal analysis of phospholipid fatty acid signatures revealed that microbial community structures in the rhizospheric and non-rhizospheric soil were similar, but different from those in the unplanted and control soil.
研究领域:
环境科学
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生态学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Jianguo Li,Wanli Yan,Shikan Zheng,Chengsong Ye,Yangying Zhao,Xin Yu
Membrane types are known to have a decisive effect on the operational efficiency of membrane-bound reactors. The gas-permeable membrane, serving as the biofilm growth and oxygen transfer medium, plays a vital role in the membrane-aerated biofilm reactor (MABR). However, few studies have thoroughly investigated how membrane types affect MABR performance. Four parallel MABRs were equipped with different membrane types: microporous PTFE (R1), dense silicone (R2), composite A (R3), and composite B (R4) membranes. Results showed that the R3 and R4 exhibited comparable and superior COD and nitrogen removal efficiency relative to R1, maintaining stable transmembrane pressure. XDLVO theory was used to assess the interfacial interaction energy between sludge flocs and membrane surfaces. Polar forces were crucial for sludge adhesion, affecting biofilm formation and growth. Composite B exhibited the lowest energy barrier (2529 KT), leading to the fastest biofilm formation and thicker biofilms, which enhance nitrogen removal. PTFE exhibited the highest energy barrier (9655 KT), resulting in the most extended biofilm cultivation period and the thinnest biofilm. Based on fluorescent in-situ hybridization (FISH), membrane types significantly affect the distribution of nitrogen-removing microbial communities. As the biofilm thickened, the stratification of the nitrifying bacterial population became increasingly pronounced, forming distinct layers within the biofilm. Nitrosomonas and Nitrobacter dominated the biofilm near the membrane, while Nitrospira was most abundant in the outer biofilm. The outermost biofilm layer was predominantly composed of heterotrophic denitrifying bacteria (HDB). The research has shown that different biofilm types exhibit heterogeneity stratification in nitrifying bacteria and HDB. 16S rRNA genes sequencing revealed that Nitrospira was the predominant nitrifying genus within the heterotrophic/autotrophic biofilm.
研究领域:
环境工程
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微生物学
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DOI
高影响因子
ENVIRONMENTAL RESEARCH
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2024
·
IF 7.7
Xingyao Ye,Xiaojun Niu,Ling Li,Mengyu Lv,Dongqing Zhang,Deye Chen,Yu Line,Zhiquan Yang
N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is an antioxidant commonly used in tire manufacturing, and its release into the environment has significantly increased due to rapid urbanization. When subjected to ozonation, 6PPD converts into the harmful pollutant 6PPD quinone (6PPDQ). These substances enter wastewater treatment plants (WWTPs) via stormwater runoff and pipelines, posing significant risks to the functional microorganisms. Anammox, a strictly controlled and sensitive microbial nitrogen removal process, is especially susceptible to the effects of the pollutants. This study investigates the comprehensive impact of 6PPD-Q and 6PPD on anammox communities based on characterization analysis and metagenomics. At environmental concentrations, 6PPD-Q at 200 ng/L-1000 ng/L led to the disintegration of anammox granules. Extended exposure to both 6PPD-Q and 6PPD significantly reduces the population of anammox bacteria (AnAOB). By utilizing organic matter from dead cells and incoming carbonate as a carbon source, the system evolved into a nitrogen metabolism network primarily focused on denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This transformation was accompanied by a reshuffling of the microbial community and associated genes, resulting in an accumulation of NH 4 + -N. These findings underscore the toxicity of 6PPD-Q and 6PPD to anammox and stress the importance of incorporating 6PPD into regulatory and preventive strategies.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Shuai Zhao,Chengtuo Niu,Jingyi Suo,Youlong Zan,Yuehu Wei,Feiyun Zheng,Chunfeng Liu,Jinjing Wang,Qi Li
‘Bask in daytime and dewed at night’ technique is traditionally used in doubanjiang fermentation, however, the underlying mechanism behind this technique is still unrevealed. This study aimed to elaborate the roles of three key indexes in this technique, that are air, sunlight, and mash turnover, on doubanjiang quality through the design of doubanjiang fermentation. The results showed that the exposure to air or sunlight was beneficial for doubanjiang to yield reddish-brown luster color, less acidity and balanced flavor. On the contrary, blocking of air and sunlight could accumulate higher acidity (4.75 ± 0.01 g/100g dw ), amino nitrogen (2.08 ± 0.07 g/100g dw ), alcohols (36.9 g/kg) and esters (26.9 g/kg). Regular mash turnover was mainly involved in making different mash layers exposure to air or sunlight equally through homogenizing mash layers. Different conditions also have been suggested to affect the microbiota dynamics by amplicon sequencing and RT-qPCR. Based on RDA analysis, Pediococcus , Meyerozyma , Bacillus and Zygosaccharomyces were predicted to be related to the changes of physicochemical properties or volatile compounds in different conditions, and their function was validated by simulated fermentation. This study would unravel the underlying scientific meaning of ‘bask in daytime and dewed at night’ technique in doubanjiang fermentation.
研究领域:
食品科学
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微生物学
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产品关联
DOI
高影响因子
Environmental Science-Processes & Impacts
·
2015
·Vol. 17
·期 12
·2022-2033
·
IF 2.171
Tiantian Shen,Yongrui Pi,Mutai Bao,Nana Xu,Yiming Li,Jinren Lu
The efficiencies of free and immobilized microbial consortia in the degradation of different types of petroleum hydrocarbons were investigated. In this study, the biodegradation rates of naphthalene, phenanthrene, pyrene and crude oil reached about 80%, 30%, 56% and 48% under the optimum environmental conditions of free microbial consortia after 7 d. We evaluated five unique co-metabolic substances with petroleum hydrocarbons, α-lactose was the best co-metabolic substance among glucose, α-lactose, soluble starch, yeast powder and urea. The orthogonal biodegradation analysis results showed that semi-coke was the best immobilized carrier followed by walnut shell and activated carbon. Meanwhile, the significance of various factors that contribute to the biodegradation of semi-coke immobilized microbial consortia followed the order of: α-lactose > semi-coke > sodium alginate > CaCl2. Moreover, the degradation rate of the immobilized microbial consortium (47%) was higher than that of a free microbial consortium (26%) under environmental conditions such as the crude oil concentration of 3 g L−1, NaCl concentration of 20 g L−1, pH at 7.2–7.4 and temperature of 25 °C after 5 d. SEM and FTIR analyses revealed that the structure of semi-coke became more porous and easily adhered to the microbial consortium; the functional groups (e.g., hydroxy and phosphate) were identified in the microbial consortium and were changed by immobilization. This study demonstrated that the ability of microbial adaptation to the environment can be improved by immobilization which expands the application fields of microbial remediation.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Mengmeng Li,Canping Pan,Zhijia Zhang,Jialing Wang,Shuai Wang,Wenzhuo Li,Tianbing Zhou,Xiaoyi Wang,Ziyi Liu,Zhan Hu,Ranfeng Sun,Dong Li
Imidacloprid (IMI) stress positively correlates with the potential of coumarins to alleviate abiotic stress. However, little is known about the pathways and mechanisms by which coumarin reduces the IMI residue by regulating plant secondary metabolism and plant–microbe interactions. This study examined the impact of coumarin on the uptake, translocation, and metabolism of IMI in pepper plants by modulating the signal molecule levels and microbial communities in the rhizosphere and phyllosphere. Analysis of 2 h─28 d pesticide residue dynamics revealed that coumarin dramatically reduced IMI concentration in pepper fruits. Coumarin upregulated the phenylpropane pathway genes, which increased the levels of flavonoids, phenolic acids, phytohormones, and capsaicinoids. Importantly, phyllosphere and rhizosphere microbial diversity results showed that coumarin improved the abundance of beneficial microorganisms and positively correlated with secondary metabolite secretion. Therefore, coumarin exploited the interaction between the phenylpropane and coumarin synthesis pathways and beneficial microbes to enhance the nutritional quality and IMI degradation.
研究领域:
植物学
·
环境科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Haozhen Li,Shuyao Wang,Xiaohua Zhang,Kangkang Song,Long Yang
Tea quality in greenhouse was certain gap with open air. Metabolites and foliar microorganisms were investigated under seaweed fertiliser (CF) and gibberellin (CH) treatments using sensory evaluation, HPLC, untargeted metabolomics, 16S rDNA, and Internal Transcribed Spacer. CF tea was mellow, less astringent, and of better quality compared to CH. Catechin, −(−)Epicatechin, and Epigallocatechin were notably lower in CF. Differentially accumulated metabolites (DAMs) were notably enriched in Flavonoid and Phenylpropanoid biosynthesis, both involved in Catechin synthesis. DAMs in these pathways appeared down-regulated in CF. The CF improved quality by down-regulating metabolites in Phenylpropanoid biosynthesis in conjunction with microbial community metabolism enriched in amino acid and secondary metabolite biosynthesis. Metabolite- microbial correlation analysis indicated that the highest correlation with phenylpropane pathway metabolites was in bacteria Variovorax and Pseudomonas , and in fungi Filobasidium . The study provides theoretical basis for regulating flavour quality of greenhouse tea.
研究领域:
植物学
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代谢组学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ACS ES&T Water
·
2025
·
IF 4.3
Yingru Tao,Weiying Feng,Qiwei Gu,Tengke Wang,Fanhao Song,Tingting Li
Microbial communities serve as crucial mediators of dissolved organic matter (DOM) dynamics in seasonally ice-covered lakes. This comparative study of a saline plateau lake (Lake Daihai) and an eutrophic freshwater lake (Lake Wuliangsuhai) revealed distinct winter microbial patterns, with sediment communities exhibiting 2–3 times higher diversity than ice or water habitats. Fluorescence characterization identified fundamental differences in DOM sources, where saline lake DOM showed mixed terrestrial-autochthonous signatures (fluorescence index: 1.4–1.9), contrasting with the strongly microbial-derived DOM (FI > 1.9) in freshwater systems. Vertical profiling demonstrated progressive humification downward through ice layers, while microbial functional predictions indicated sustained amino acid metabolism despite subzero temperatures. The unique halotolerant community structure of the saline lake contrasted sharply with the psychrophilic Flavobacterium-dominated assemblages in freshwater habitats, reflecting differential adaptation to environmental stressors. Dissolved oxygen and salinity emerged as primary drivers of community composition, while phosphorus availability influenced specific functional groups involved in organic matter processing. These findings highlight the resilience of microbial-mediated carbon cycling under ice cover and provide critical baseline data for predicting ecosystem responses to changing winter conditions in inland waters.
研究领域:
生态学
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环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）