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微生物诱导碳酸钙沉淀
Journal of Marine Science and Engineering · 2025 ·Vol. 13 ·期 5 ·883 · IF 2.8

Experimental Study on the Reinforcement of Calcareous Sand Using Combined Microbial-Induced Carbonate Precipitation (MICP) and Festuca arundinacea Techniques

Xiuqiong Deng,Ziyu Wang,Yuchun Qin,Liang Cao,Peng Cao,Yu Xie,Yingqi Xie

Combining the Microbial-Induced Calcium Carbonate Precipitation (MICP) technique with plants to reinforce calcareous sand slopes on reef islands is expected to achieve both reinforcement and economic benefits. In this study, MICP was combined withFestuca arundinacea(MICP-FA) for calcareous sand reinforcement. Based on water retention and scanning electron microscopy (SEM) tests, the water retention performance and mechanism of MICP-reinforced calcareous sand under different cementation solution concentrations and cementation cycles were analyzed. The growth adaptability ofFestuca arundinaceawas evaluated under different bacteria solution concentrations, cementation solution concentrations and cementation cycles. The engineering applicability of MICP-FA-reinforced calcareous sand was evaluated by wind erosion tests, and the synergistic reinforcement mechanism was analyzed. The results show that with the increase in the cementation solution concentration and cementation cycles, more calcium carbonate filled and adhered to the calcareous sand particles, significantly improving the water retention performance. MICP-FA can enhance the wind erosion resistance of calcareous sand. This synergistic mechanism lies in the surface cementation effect of MICP and the deep anchoring effect of plant roots. This study provides theoretical basis and technical guidance for engineering applications in calcareous sand areas.

CAS / MDL: 57-13-6
研究领域: 土力学 · 环境工程 · 生态修复
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CONSTRUCTION AND BUILDING MATERIALS · 2021 · IF 6.141

Effect of nano-silica on microbiologically induced calcium carbonate precipitation

Miao Liu,Lixiong Cai,Hanbin Luo

Microbiologically induced calcium carbonate precipitation (MICP) is a new technique in environmental geotechnical engineering. Ultrafine particles including nanoparticles consisting of clay particles and organic matter present in the nanometer size range are ubiquitous in sand due to the erosion or chemical reactions of earth minerals. In this paper, nano-SiO 2 (NS) was used to represent nanoparticles and investigate the influence of nanoparticles in soil on MICP. Both MICP in a solution environment and MICP on the surface of quartz sand were investigated. Then, a series of macroscopic and microscopic tests were conducted to investigate the effect mechanism of NS on MICP. It was found that the NS could inhibit the MICP reaction process and promote calcite formation in a solution environment. Conversely, NS could inhibit calcite formation on the quartz sand surface. Besides, the main products of these two cases are quite different: the former is vaterite, while the latter is calcite. In general, the nanoscale silica particles are detrimental to MICP.

研究领域: 环境岩土工程 · 材料科学 · 地球化学
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Journal of Environmental Chemical Engineering · 2023 · IF 7.7

The optimized microbial-induced calcium carbonate precipitation process to fabricate underwater superoleophobic mesh for efficient oil-water separation

Sikai Tang,Yuze Wang

Microbial-induced calcium carbonate precipitation (MICP) is an environmentally-friendly and easy operation technique for separating oil-water mixtures. However, the effect of environmental factors on the wettability and oil-water separation performance of MICP remains unclear. In this study, three primary factors including mineralization solution (MS) concentration, bacterial suspension (BS) concentration, and temperature were carefully investigated. Under these varying conditions, stainless steel meshes (SSM) were chosen as the substrates for the growth of CaCO 3 precipitations. Experimental results demonstrate a strong dependence of the morphology and wettability of CaCO 3 precipitations on these factors. Higher MS concentration and temperature contribute to enhanced hydrophilicity of CaCO 3 coatings due to the increased surface roughness . The water flux of MICP-coated SSMs decreases with the increase of BS concentration, MS concentration, and temperature. The MICP-coated SSM obtained with MS concentration of 0.15 M, BS concentration of 0.1 × 10 8 cells/mL, and mineralization reaction at 25 ℃ for 24 hours demonstrated the optimal comprehensive performance, exhibiting underwater superoleophobicity with an underwater oil contact angle (UWOCA) exceeding 151°, an ultra-high oil intrusion pressure (> 3.0 kPa), ultrahigh flux (28,644 L m −2 h −1 ), and a preferable oil rejection rate (> 99.9%). Additionally, the as-prepared mesh exhibited superior anti-oil-fouling property and reusability, while concurrently demonstrating excellent chemical and thermal stability. This work contributes to advancing our knowledge of the influence of various environmental factors on the wetting characteristics of MICP and provides a feasible solution for the highly efficient and low-cost preparation of oil-water separation membranes based on the MICP process.

研究领域: 环境科学 · 材料科学
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GEOMICROBIOLOGY JOURNAL · 2025 · IF 1.9

Influence and Mechanism of pH and Temperature on Microbial Carbonic Anhydrase Production and Activity in Carbon Sequestration and Self-Healing Concrete

Chunxiang Qian,Xian Hu,Yaya Yuan,Junjie Zhang,Yijin Fan

Microbial induction of carbonate precipitation is common in nature, carbonic anhydrase (CA) secreted by microorganisms plays an important role in catalyzing the precipitation of CaCO3 from CO2. This study investigated how pH and temperature regulate microbial CA production, activity, and structure, and further examined their effects on CaCO3 deposition, steel slag carbon sequestration, and concrete crack healing. The results demonstrated that bacteria efficiently produced CA and maintained its activity over a broad range of alkaline pH levels and temperatures, the optimal conditions pH 9 and 30°C facilitated both bacterial CA production and activity by regulating growth and metabolism. Furthermore, pH and temperature modulated CA activity by altering its secondary structure (α-helices, β-turns) and weakening the enzyme’s active center for CO2 at higher pH, which consequently influenced CaCO3 deposition efficiency. The microbial treatment significantly enhanced carbon fixation in steel slag, with an 18.9% carbon sequestration rate at 48 hours, an 8% increase over the control group. In concrete crack-healing tests, the application of alkali-resistant microorganisms restored water permeability resistance to 93.24% of the original concrete after 28 d of healing. Microbial mineralization technology has great potential in steel slag carbon sequestration and self-healing concrete.

CAS / MDL: 73049-73-7
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JOURNAL OF HAZARDOUS MATERIALS · 2021 · IF 10.588

Microbially induced calcium precipitation based simultaneous removal of fluoride, nitrate, and calcium by Pseudomonas sp. WZ39: Mechanisms and nucleation pathways

Zhao Wang,Junfeng Su,Amjad Ali,Ruijie Zhang,Wenshuo Yang,Liang Xu,Tingbao Zhao

A simultaneous denitrifying and mineralizing bacterium, Pseudomonas sp. WZ39 was isolated for fluoride (F − ), nitrate (NO 3 − -N), and calcium (Ca 2+ ) removal. Strain WZ39 exhibited a remarkable defluoridation efficiency of 87.49% under a pH of 6.90, F − and Ca 2+ concentration of 1.99 and 201.88 mg L −1 , respectively. EEM, SEM–EDS, XRD, and FTIR analyses elucidated the chemical adsorption and co-precipitation with calcium salt contributed to the removal of F − . The mechanisms of biomineralization were also investigated by determining the role of bound and unbound extracellular polymeric substances (EPS), cell wall, and calcium channel in nucleation. The results showed that bacteria can promote nucleation on the templates of cell walls or EPS through the electrostatic effect. The presence of the calcium channel blocker inhibited the transport of intracellular Ca 2+ to the extracellular environment. The outcome of the present research can provide a theoretical basis for the understanding of MICP phenomenon and the efficient treatment of F − containing groundwater.

研究领域: 环境微生物学 · 水处理工程 · 生物地球化学
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Journal of Water Process Engineering · 2024 · IF 6.3

Mesh coated with microbially induced nanoscale vaterite for oil-in-water emulsion separation

Sikai Tang,Yuze Wang

Microbial-induced calcium carbonate precipitation (MICP), with its hydrophilic rough microstructures and as an environmentally friendly material modification method, has shown great potential for oil-water separation. However, its efficiency in separating oil-in-water emulsions remains challenging. This study advances the MICP method by producing microbially induced nanoscale calcium carbonate on a mesh using calcium acetate instead of traditional calcium chloride, successfully achieving efficient separation of oil-in-water emulsions. The stainless-steel mesh (SSM) after calcium acetate-MICP treatment obtained nano-elliptical flake-like vaterite (vaterite-SSM) and demonstrated superior superhydrophilicity and underwater superoleophobicity compared to the mesh coated with micro-cubic structured calcite (calcite-SSM) treated by calcium chloride-MICP. Notably, vaterite-SSM achieved a flux of up to 309 L·m −2 ·h −1 and an oil rejection rate of over 98.7 % in gravity-driven separation of oil-in-water emulsions, demonstrating significant reusability after 6 cycles. Conversely, calcite-SSM was ineffective in emulsion separation due to coating instability and large particle spacing. Additionally, vaterite-SSM effectively separated various oil-water mixtures, maintaining high performance across 30 separation cycles. This study underscores the important use of calcium acetate in MICP to obtain nano-elliptical flake-like vaterite for efficient oil-water emulsion separation, advancing the promise of MICP as an effective and sustainable method for environmental applications.

研究领域: 环境科学 · 材料科学 · 化学工程
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