iScience
·
2024
·
IF 4.6
Ziyang Gao,Erqian Huang,Wanting Wang,Lizhong Xu,Wanyue Xu,Ting Zheng,Menglong Rui
Summary Synapses are fundamental components of the animal nervous system. Synaptic cytoskeleton is essential for maintaining proper neuronal development and wiring. Perturbations in neuronal microtubules (MTs) are correlated with numerous neuropsychiatric disorders. Despite discovering multiple synaptic MT regulators, the importance of MT stability, and particularly the polarity of MT in synaptic function, is still under investigation. Here, we identify Patronin, an MT minus-end-binding protein, for its essential role in presynaptic regulation of MT organization and neuromuscular junction (NMJ) development. Analyses indicate that Patronin regulates synaptic development independent of Klp10A. Subsequent research elucidates that it is short stop (Shot), a member of the Spectraplakin family of large cytoskeletal linker molecules, works synergistically with Patronin to govern NMJ development. We further raise the possibility that normal synaptic MT polarity contributes to proper NMJ morphology. Overall, this study demonstrates an unprecedented role of Patronin, and a potential involvement of MT polarity in synaptic development.
研究领域:
神经生物学
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细胞生物学
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发育生物学
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 5
·1537
·
IF 4.927
Juan Ma,Guo-Hua Gong
In this paper, a small series of novel quinoline sulfonamide derivatives was synthesized, and their structure of the target compounds were confirmed by 1H NMR and MS. The screening of the news target compounds’ in vitro cytotoxic activities against tumor cell lines by the MTT method was performed. Among them, compoundD13(N-(4-methoxybenzyl)-2-oxo-N-(3,4,5-trimethoxyphenyl)-1,2,3,4-tetrahydroquinoline-6-sulfonamide exhibited the strongest inhibitory effect on the proliferation of HeLa (IC50: 1.34 μM), and this value correlated well with the inhibitory activities of the compound against tubulin polymerization (IC50: 6.74 μM). In summary, a new type of quinoline-sulfonamide derivative with tubulin polymerization inhibitory activity was discovered, and it can be used as a lead compound for further modification.Keywords:synthesis;quinoline-sulfonamide;anti-tumor;tubulin polymerization
研究领域:
药物化学
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肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Zhenyu Wang,Yu Huang,Meijuan Chen,Xianjin Shi,Yufei Zhang,Junji Cao,Wingkei Ho,Shun Cheng Lee
The development of catalysts that effectively activate target pollutants and promote their complete conversion is an admirable objective in the environmental photocatalysis field. In this work, graphitic carbon nitride (g-C3N4) microtubes with tunable N-vacancy concentrations were controllably fabricated using an in situ soft-chemical method. The morphological evolution of g-C3N4, from the bulk to the porous tubular architecture, is discussed in detail with the aid of time-resolved hydrothermal experiments. We found that the NO removal ratio and apparent reaction rate constant of the g-C3N4 microtubes were 1.8 and 2.6 times higher than those of pristine g-C3N4, respectively. By combining detailed experimental characterization and density functional theory calculations, the effects of N-vacancies in the g-C3N4 microtubes on O2 and NO adsorption activation, electron capture, and electronic structure were systematically investigated. These results demonstrate that surface N-vacancies act as specific sites for the adsorption activation of reactants and photoinduced electron capture, while enhancing the light-absorbing capability of g-C3N4. Moreover, the porous wall structures of the as-prepared g-C3N4 microtubes facilitate the diffusion of reactants, and their tubular architectures favor the oriented transfer of charge carriers. The intermediates formed during photocatalytic NO removal processes were identified by in situ diffuse reflectance infrared Fourier transform spectroscopy, and different reaction pathways over pristine and N-deficient g-C3N4 are proposed. This study provides a feasible strategy for air pollution control over g-C3N4 by introducing N-vacancy and porous tubular architecture simultaneously.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Yanqin Xue,Qing Yan,Xiaojing Bai,Yanyan Xu,Xuan Zhang,Yiju Li,Kai Zhu,Ke Ye,Jun Yan,Dianxue Cao,Guiling Wang
The development of efficient bifunctional catalysts for both hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is essential for reducing the cost of hydrogen production by water splitting. Herein, hollow microtubes composed of RuNi 1 Co 1 alloy nanoparticles uniformly embedded in the carbon matrix (RuNi 1 Co 1 @CMT) are prepared through a simple impregnation followed by reduction. Benefiting from the unique mosaic structure and the synergistic effect between Ru and NiCo, RuNi 1 Co 1 @CMT achieves more exposed active sites and improved reaction kinetics. As a consequence, RuNi 1 Co 1 @CMT exhibits considerable catalytic activities with the overpotentials of 78 mV for HER and 299 mV for OER at 10 mA cm −2 in 1 M KOH. In addition, RuNi 1 Co 1 @CMT exhibits excellent stability for up to 30 h in both HER and OER processes at 20 mA cm −2 , which is attributed to the protection of the RuNi 1 Co 1 alloy particles by the carbon layer. Furthermore, the assembled RuNi 1 Co 1 @CMT || RuNi 1 Co 1 @CMT overall water splitting system shows a cell voltage of 1.58 V at 10 mA cm −2 . The density functional theory (DFT) calculations indicate that the addition of Ru can optimize the hydrogen adsorption free energy of Ni and Co sites. Finally, a solar panel-driven water splitting device is built, which can realize green and sustainable hydrogen production. The fabrication of RuNi 1 Co 1 @CMT provides a new way for the preparation of effective alloy nanomaterials for energy storage and conversion.
研究领域:
材料科学
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催化化学
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能源转换
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OSTEOARTHRITIS AND CARTILAGE
·
2024
·
IF 7.2
Jia Yu,Qian Liu,Yuejiao Zhang,Lingfeng Xu,Xiaohua Chen,Feng He,Mian Zhang,Hongxu Yang,Shibing Yu,Xin Liu,Yaoping Wu,Meiqing Wang
Objective Abnormal mechanical stress is intimately coupled with osteoarthritis (OA). Microtubules play a vital role in the regulation of mechanotransduction and intracellular transport. The purpose of the present study was to investigate the impact of stress-induced microtubule impairment on intracellular transport and lipid droplet (LD) accumulation in chondrocytes. Method Rats were subjected to unilateral anterior crossbite (UAC), which is inducible for degeneration of temporomandibular joint (TMJ) cartilage. Chondrocytes derived from rat TMJ cartilage were subjected to fluid flow shear stress (FFSS) and analyzed via LC MS/MS-based proteomics. The microtubule destabilization agent nocodazole and/or the microtubule stabilizer docetaxel were used in the UAC and FFSS models. Results In both FFSS- and UAC-treated chondrocytes, decreased acetylated α-Tubulin (ac-Tubulin) expression and LD accumulation were observed. Proteomic data revealed increased levels of the LD-associated protein perilipin 3 (Plin3) and decreased levels of cytoskeleton components in FFSS-treated chondrocytes. Live-cell imaging revealed that the colocalization of LDs with lysosomes was significantly decreased after FFSS treatment. Impairment of microtubules by nocodazole reduced the protein level of ac-Tubulin and disrupted the Hsc70-mediated interaction between Plin3 and Lamp2a, as shown by co-IP assays. In contrast, docetaxel reversed the suppression of ac-Tubulin expression, reduced the accumulation of LDs, and decreased the expression of Plin3 in chondrocytes exposed to FFSS and UAC, and docetaxel ameliorated UAC-induced osteoarthritic lesions in the TMJ cartilage. Conclusion Microtubule impairment under aberrant stress conditions disrupts intracellular transport and blocks lipophagy, causing LD accumulation in chondrocytes. Microtubule stabilization could be a new approach for treating stress-induced cartilage degeneration.
研究领域:
医学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 40
·12704-12708
·
IF 6.641
Shaozuo Huang,Huan Chen,Tao He,Changjian Zhang,Chengyun Zhang,Zhengkun Fu,Zhenglong Zhang,Hairong Zheng
Upconversion luminescence-based waveguides can achieve optical signal transmission and visible light emission with near-infrared light excitation and their quality is highly dependent on the coupling efficiency between the light and waveguide. Here, a new luminescent waveguide formed by a rare-earth doped microtube with beveled ends was designed to increase the optical coupling efficiency for vertical waveguide-excitation. The beveled end enabled the near-infrared light to effectively couple into the waveguide and produce a visible transmission pattern and directional emission, which was not possible using conventional luminescent waveguides without beveled ends. It was found that the beveled end can not only improve the input and the output signal efficiency, but also control the coupling efficiency and transmission pattern. In particular, a coupling efficiency of ∼68% could be obtained with the optimized beveled waveguide with a rotation angle of 30°. The high optical coupling efficiency and multi-channel luminescent waveguide enabled by the microtube with beveled ends allowed for a deeper understanding of vertical waveguide excitation, and it extends the potential applications of luminescent waveguides for bioimaging and optical communication.
研究领域:
光学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Science China-Chemistry
·
2025
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IF 9.7
Zeng Si-Min,Zheng Di-Wei,Pan Ting,Ding Xing-Lan,Zhang Yun,Yang Chi-Hui,Cheng Si-Xue,Zhang Xian-Zheng
Natural biological microtubular networks have undergone adaptive evolutionary selection and may offer viable solutions to design challenges in artificial microtubular networks. The plasmodium of the slime mold Physarum polycephalum ( P. polycephalum ) extends continuously to form a protoplasmic microtubular network structure, directly connecting food sources. Computational simulations revealed that the formation of this adaptive P. polycephalum microtubular network could be captured by a mathematical algorithm. Inspired by the P. polycephalum microtubular networks, we propose an adaptive optimization design method for artificial microtubular networks. Specifically, we utilized hydrogels with biodegradable and tissue-adhesive properties to replicate the P. polycephalum microtubular networks via photomask. In Rhodamine B diffusion and glucose-catalyzed reaction experiments, we found that the P. polycephalum microtubular networks exhibited significantly enhanced efficiency compared to vascular and artificial networks. Furthermore, we demonstrated the potential for uric acid (UA) degradation of the hydrogels with a real P. polycephalum microtubular network loaded with urate oxidase (UOx) in a rodent model of hyperuricemia. And this network achieved more than double the effect of the artificial network. This underscores the potential of natural microtubular networks to replace artificial microtubular networks.
研究领域:
生物医学工程
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材料科学
·
计算生物学
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产品关联
DOI
高影响因子
Soft Matter
·
2019
·Vol. 15
·期 42
·8468-8474
·
IF 3.399
Yangkun Du,Chaofeng Lü,Congshan Liu,Zilong Han,Jian Li,Weiqiu Chen,Shaoxing Qu,Michel Destrade
Initial residual stress is omnipresent in biological tissues and soft matter, and can affect growth-induced pattern selection significantly. Here we demonstrate this effect experimentally by letting soft tubes grow in the presence or absence of initial residual stress and by observing different growth pattern evolutions. These experiments motivate us to model the mechanisms at play when a growing bilayer tubular organ spontaneously displays buckling patterns on its inner surface. We demonstrate that not only differential growth, geometry and elasticity, but also initial residual stress distribution, exert a notable influence on these pattern phenomena. Prescribing an initial residual stress distribution offers an alternative or a more effective way to implement pattern selection for growable bio-tissues or soft matter. The results also show promise for the design of 4D bio-mimic printing protocols or for controlling hydrogel actuators.
研究领域:
生物力学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Zhi Jiang,Hezhuang Liu,Jihua Zou,Yixuan Huang,Zhaoquan Xu,Caihong Li,Desheng Liu
With the rapid advances in metal halide perovskite optoelectronics, eliminating toxic lead from perovskites has been an urgent demand. However, state-of-the-art lead-free perovskite photodetectors are still challenged with issues of low photoresponse, poor stability, etc. Here, all-inorganic lead-free perovskite (Cs3Cu2I5) single crystals that possess good stability under air exposure are synthesized via a facile solid reaction method. Meanwhile, a higher photoluminescence quantum yield of 95.2% and a prolonged carrier lifetime of 1.127 μs are obtained by further optimizing the synthesis. Benefiting from the polyporous surface and hollow structure of Cu-7,7,8,8-tetracyanoquinodimethane (CuTCNQ) microtubes, more Cs3Cu2I5 nanocrystals can adhere on the innershell and outershell of CuTCNQ-15 microtubes. This unique structure contributes to the improved efficiency of utilizing incident light and promotes charge carrier generation and transportation. As a result, the hierarchical CuTCNQ/Cs3Cu2I5 (hollow microtube/nanocrystal) heterostructure phototransistor exhibits a high responsivity of 88.36 A W–1 and a large detectivity of 1.66 × 1012Jones. The proposed lead-free perovskites and mixed-dimensional heterojunctions are promising for sensitive light detection.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2021
·
IF 5.051
Xiang Zhou,Jiamin Liu,Jiao Meng,Yihong Fu,Zhibin Wu,Guiping Ouyang,Zhenchao Wang
Microtubule dynamics are crucial for multiple cell functions, and cancer cells are particularly sensitive to microtubule-modulating agents. Here, we describe the design and synthesis of a series of (Z)-2-(5-benzylidene-4-oxo-2-thioxothiazolidin-3-yl)-N-phenylacetamide derivatives and evaluation of their microtubule-modulating and anticancer activities in vitro. Proliferation assays identified I20 as the most potent of the antiproliferative compounds, with 50% inhibitory concentrations ranging from 7.0 to 20.3 µM with A549, PC-3, and HepG2 human cancer cell lines. Compound I20 also disrupted cancer A549 cell migration in a concentration-dependent manner. Immunofluorescence microscopy, transmission electron microscopy, and tubulin polymerisation assays suggested that compound I20 promoted protofilament assembly. In support of this possibility, computational docking studies revealed a strong interaction between compound I20 and tubulin Arg β369, which is also the binding site for the anticancer drug Taxol. Our results suggest that (Z)-2-(5-benzylidene-4-oxo-2-thioxothiazolidin-3-yl)-N-phenylacetamide derivatives could have utility for the development of microtubule-stabilising therapeutic agents.
研究领域:
肿瘤学
·
分子生物学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
·
2019
·
IF 2.752
Hongfang Jiu,Na Ren,Lixin Zhang,Qing Zhang,Yuying Gao,Yajuan Meng
The flower-like ZnMn 2 O 4 hollow microtubules were synthesized by a hydrothermal method and calcination method. The composition and morphology were characterized. The results showed that flower-like hollow microtubules were obtained through calcination treatment. The as-prepared ZnMn 2 O 4 electrochemical properties of flower-like hollow microtubules anode material for lithium-ion batteries were investigated. The first charge and discharge capacity was measured at 2152/1263 mA h g −1 when the current density at 400 mA g −1 . After 300 cycles, the capacity kept 515 mA h g −1 corresponding to the coulombic efficiency of 99%, which exhibited preferable cycling performance. The stability of the structure has been shown by rate performance, which had been tested under different current density. The results suggest a promising application of the ZnMn 2 O 4 as anode material for lithium-ion batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
NANO
·
2014
·
IF 1.26
JUN LI,BIN KANG,SHUQUAN CHANG,YAODONG DAI
We directly visualized the uptake and intracellular vesicle transport of shortened single walled carbon nanotubes in living cells through a dual-labeling system. With a stable labeling of fluorescein isothiocyanate and a pH-sensitive stacking of doxorubicin on carbon nanotubes, the location of internalized nanotubes inside the cell and the microenvironment especially the pH change of the nanotube-embedded vesicles could be monitored at the same time. Results showed that after internalization through endocytic pathway, carbon nanotubes tended to transport from the early endosomes to later acidic lysosomes and these vesicles moved along the microtubule track toward a perinuclear region where is a microtubule-organizing center. These results might provide a novel understanding for the intracellular interaction of carbon nanotubes and living cells.
研究领域:
细胞生物学
·
纳米医学
·
生物成像
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）