Polymers
·
2022
·Vol. 14
·期 7
·1370
·
IF 4.967
Xingyu Yuan,Wen Li,Bin Yao,Zhao Li,Deling Kong,Sha Huang,Meifeng Zhu
Bionic grafts hold great promise for directing tissue regeneration. In vascular tissue engineering, although a large number of synthetic grafts have been constructed, these substitutes only partially recapitulated the tri-layered structure of native arteries. Synthetic polymers such as poly(l-lactide-co-ε-caprolactone) (PLCL) possess good biocompatibility, controllable degradation, remarkable processability, and sufficient mechanical strength. These properties of PLCL show great promise for fabricating synthetic vascular substitutes. Here, tri-layered PLCL vascular grafts (TVGs) composed of a smooth inner layer, circumferentially aligned fibrous middle layer, and randomly distributed fibrous outer layer were prepared by sequentially using ink printing, wet spinning, and electrospinning techniques. TVGs possessed kink resistance and sufficient mechanical properties (tensile strength, elastic modulus, suture retention strength, and burst pressure) equivalent to the gold standard conduits of clinical application, i.e., human saphenous veins and human internal mammary arteries. The stratified structure of TVGs exhibited a visible guiding effect on specific vascular cells including enhancing endothelial cell (EC) monolayer formation, favoring vascular smooth muscle cells’ (VSMCs) arrangement and elongation, and facilitating fibroblasts’ proliferation and junction establishment. Our research provides a new avenue for designing synthetic vascular grafts with polymers.Keywords:biomimetic;multilayer structure;smooth surface;aligned microfiber;random microfiber
研究领域:
组织工程
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Rui Yuan,Kai Wu,Qiang Fu
Direct ink writing, as a prospective three-dimensional (3D) printing technique, opens opportunities for offering high freedom to process cellulose into complex shapes. Current challenges are the accomplishment of dimensional accuracy of printed object and its truly 3D configuration, which put stringent requirements to the ink rheology that should be easily flowable under a suitable shear force while can rapidly recover to solid state after the force is removed. In this study, benefiting from the exquisite regulation of residual cellulose microfiber in a dissolved cellulose solution, a remarkable ink with excellent thixotropic property is prepared, which elaborately balance the traditional rheology tradeoff (extrudability and self-supporting) upon the formation of interconnected microfiber network. As a result, the optimized cellulose ink allows the printed object to successfully display arbitrary and truly 3D configurations, such as anatomical heart, ear, rabbit, lamp, and cup, and exhibit fine microstructure with a resolution of 250 μm.
研究领域:
材料科学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SUPERCRITICAL FLUIDS
·
2021
·
IF 4.577
Haoxiang Zhang,Guangmeng Li,Jingbo Yang,Ai-zheng Chen,Maobin Xie
Poor solubility limits anticancer efficiency of Artemisinin (ART). Here, two types of micronized ART products were produced in one system, using supercritical CO 2 . ART microfibers (0.590 µm ± 0.160 µm) were obtained at a pressure of 25 MPa, temperature of 55 °C, and concentration of 4 mg/mL. ART microparticles (0.890 µm ± 0.610 µm) were obtained at a pressure of 20 MPa, temperature of 55 °C, and decompression time of 60 min. Importantly, the viabilities of U87 cells were 39.8% ± 2.33% and 35.3% ± 11.8%, respectively; while the viabilities of 4T1 cells were 46.2% ± 4.41% and 30.2% ± 3.74%, respectively, when treated with ART microfibers and microparticles, which could be explained by cell cycle arrest in the S phase associated with apoptotic cell induction. In conclusion, supercritical-derived ART microproducts showed improved anticancer effects.
研究领域:
药理学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
Materials Research Express
·
2018
·Vol. 5
·期 4
·045019
·
IF 1.151
Shu-Xin Yu,Jie Zheng,Xu Yan,Xiao-Xiong Wang,Guang-Di Nie,Ye-Qiang Tan,Jun Zhang,Kun-Yan Sui,Yun-Ze Long
In conventional solution electrospinning, the morphologies (e.g., spheres, beaded fibers, and fibers) of electrospun products can be controlled by solution concentration. Here, we report that the morphologies and structures of polylactic acid (PLA) via melt electrospinning also can be adjusted from microfibers to microspheres by simply increasing the spinning temperature. It was found that with temperature increasing from 200 °C to 240 °C, the average diameter of melt-electrospun PLA fibers decreased from 58.46 to 2.96 μm. Then, beaded fibers and microspheres about 14.5 μm in diameter were collected when the spinning temperature was increased to 250 °C and 260 °C. In addition, we also found that the average PLA fiber diameter decreased with increasing the applied spinning voltage, and increased with the increase of spinning distance. To explain the formation mechanism of different PLA microstructures, rheological property and infrared spectra of PLA under different spinning temperatures were also tested.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2016
·
IF 5.267
Beili Lu,Fengcai Lin,Xin Jiang,Jiajia Cheng,Qilin Lu,Jianbin Song,Chong Chen,Biao Huang
A facile, environmentally benign approach has been developed for the preparation of dynamic, multiresponsive, and self-healing hydrogels from inexpensive bamboo pulp, poly(vinyl alcohol) (PVA), and borax. The microfibrillated cellulose (MFC) reinforced PVA–borax hydrogels were produced through a one-pot route in conjunction with ball milling and physical blending in tandem in aqueous medium. In this way, MFC particles could be efficiently generated and well-dispersed in a polymer matrix, and they have been verified by scanning electron microscopy. The rheology analysis indicated a close relationship between the mechanical strength and the MFC loading and ball milling time. Due to the dynamic equilibrium of the didiol–borax linkages and the reinforcement of MFC fibers, the hydrogels showed enhanced self-healing behavior and mechanical stiffness, which was also supported by rheology analyses. In addition, the hydrogels were found to be sensitive to the pH value. The hydrogels present a solvent or gel state with the change of pH value, and this sol–gel transfer can be repeated while maintaining the shape, further demonstrating the dynamic reversible behavior of the hydrogels.
研究领域:
材料科学
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2019
·
IF 2.121
Libing Huang,Kuan Cao,Ping Hu,Yong Liu
How to improve the bioavailability of the Sanguis Draconis (SD) is an important problem in the potential clinical applications. The aim of this study was to develop a drug delivery system to achieve high bioavailability of SD, a drug with poor water solubility. It will promote the research about new formulations of the SD and the other insoluble drugs. In this study, a highly biocompatible hydrophilic polymer, polyvinylpyrrolidone (PVP), was selected as a carrier, mixed with different proportions of SD to produce SD-PVP microfibers by solution electrospinning. By orthogonal experiments, the optimal spinning conditions of the preparation of SD-PVP fibers were investigated. The morphology of different proportions of SD-PVP microfibers was observed by scanning electron microscopy, and the phase characteristics were characterized by Fourier transform infrared spectrometry, X-ray diffraction, and differential scanning calorimetry. The hydrophilic properties of SD-PVP fiber membranes with different SD content were analyzed by the water contact angle assay. In vitro dissolution experiments were carried out to observe the dissolution of drugs in SD-PVP fiber membranes. The results showed that the diameter of SD-PVP fibers increased with the enlargement of SD content. A eutectic mixture was formed after blending PVP and SD, and the hydrogen bonds were formed between the SD and PVP with no chemical reaction occurred. The dispersion of SD in the fiber decreased with the increase of SD content. The higher the content of SD in the fiber, the more hydrophobic the fiber membrane. In vitro dissolution studies revealed that the dissolution content of SD from SD-PVP microfibers was significantly higher than that of the pure or original drug SD. However, as the SD content increased from 15% to 30%, the dissolution of the drug in the SD-PVP fibers decreased. The SD-PVP fiber prepared in this study showed much higher solubility than the original drug in vitro, which has great significance for the development of new dosage forms for the clinical application of SD, and it has a useful reference for the study of similar bioavailability of poorly soluble drugs.
研究领域:
药剂学
·
材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 137
·期 23
·48788
·
IF 2.188
Zhimei Wei,Fang Qiu,Xiaojun Wang,Shengru Long,Jie Yang
Conductive electrospun polymer fibers have attracted a great deal of interest in recent years. This study describes the preparation of electrically conductive microfibers composed of polyethersulfone/polydopamine/silver nanoparticles (PES/PDA/Ag NPs). Ag NPs acted as conductive centers, while hydroxyl- and amino-rich functional groups and excellent adhesion properties of PDA served to connect the Ag NPs and PES microfibers. Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) showed that PDA was firmly adhered to PES microfibers. PES/PDA microfibers absorbed considerable amounts of silver ion from AgNO 3 solution, resulting in Ag NPs. X-ray diffraction, X-ray photoelectron spectroscopy, and SEM data represented the successful formation of PES/PDA/Ag NPs microfibers. Microfibers with optimal conductivity were obtained using a solution of 2% AgNO 3 at pH 9 at 50 °C for 45 min. The electrical resistivity of our PES/PDA/Ag NPs microfibers was only 202 Ω/cm, much lower than that of regular PES microfibers (2.1 × 10 9 Ω/cm). These results show that the PES/PDA/Ag NPs microfibers are suitable for use as conductive polymer fibers in electromagnetic shielding, and conductivity-sensing applications, and in flexible electronic devices and biosensors. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137 , 48788.
研究领域:
材料科学
·
纳米技术
·
聚合物科学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 8
·1633
·
IF 3.06
Gen Wang,Luanluan Jia,Fengxuan Han,Jiayuan Wang,Li Yu,Yingkang Yu,Gareth Turnbull,Mingyu Guo,Wenmiao Shu,Bin Li
Fibrous hydrogel scaffolds have recently attracted increasing attention for tissue engineering applications. While a number of approaches have been proposed for fabricating microfibers, it remains difficult for current methods to produce materials that meet the essential requirements of being simple, flexible and bio-friendly. It is especially challenging to prepare cell-laden microfibers which have different structures to meet the needs of various applications using a simple device. In this study, we developed a facile two-flow microfluidic system, through which cell-laden hydrogel microfibers with various structures could be easily prepared in one step. Aiming to meet different tissue engineering needs, several types of microfibers with different structures, including single-layer, double-layer and hollow microfibers, have been prepared using an alginate-methacrylated gelatin composite hydrogel by merely changing the inner and outer fluids. Cell-laden single-layer microfibers were obtained by subsequently seeding mouse embryonic osteoblast precursor cells (MC3T3-E1) cells on the surface of the as-prepared microfibers. Cell-laden double-layer and hollow microfibers were prepared by directly encapsulating MC3T3-E1 cells or human umbilical vein endothelial cells (HUVECs) in the cores of microfibers upon their fabrication. Prominent proliferation of cells happened in all cell-laden single-layer, double-layer and hollow microfibers, implying potential applications for them in tissue engineering.Keywords:microfluidics;microfibers;cell-laden;tissue engineering
研究领域:
生物材料
·
组织工程
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Biobased Materials and Bioenergy
·
2023
·Vol. 17
·期 2
·225-231
·
IF 0.5
Wang Lei,Miao Lei,Chen Dong-Chu,Zhang Min
The recycling use of wood sawdust to process composite boards has received much attention in recent years. To avoid the use of chemical adhesives, natural cellulose-based materials were often used as adhesives to combine wood sawdust. Rather than utilizing nanocellulose, the report
herein describes a method to prepare a robust composite board that is based on microfibrous cellulose, which is a low-cost commercial product. To effectively cross-link wood sawdust and microfibrous cellulose, phosphoric acid-activated glutaraldehyde was used as catalyst. Compared to its uncross-linked
counterpart, the modulus of elasticity and modulus of rupture of the composite board that had been cross-linked by glutaraldehyde and phosphoric acid were significantly strengthened to 3.45±0.06 GPa and 36.5±3.5 MPa, respectively. Moreover, the moisture resistance of the cross-linked
composite board was also enhanced. After soaking in water for 24 h, the mass swelling ratio and thickness swelling ratio of cross-linked composite board had only changed 49% and 21%, respectively. These performances even exceeded that of composite board prepared from nanocellulose. The method
described in this work may have applications in commercial processing and the recycling of wood sawdust.
研究领域:
材料科学
·
林业工程
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Yufei Liu,Long Yang,Guijing Chen,Zhentao Liu,Tao Lu,Yong Yang,Jia Yu,Dongdong Kang,Wei Yan,Min He,Shuhao Qin,Jie Yu,Chuan Ye,Heng Luo
Although microfiber (MF) loaded polypeptides have been widely studied in the field of medicine, load control and slow release remain significant challenges. Herein, polybutylene adipate terephthalate (PBAT) hollow porous MFs (HPMFs) were prepared by improving and regulating the groups and structures of the MFs to address these challenges. The capacity of the HPMFs for the loading of polypeptide can be improved. Measurements involving the use of X-ray photoelectron, energy-dispersive, and ultraviolet (UV) spectroscopies in conjunction with scanning electron microscopy measurements showed that the amount of polypeptide (arginylglycylaspartic acid, RGD) loaded on the HPMFs was significantly higher than that loaded on the MFs. Animal cell experiments revealed that PBAT grafted MAH has good bioactivity. By loading RGD onto HPMFs and MFs, RGD@HPMFs and RGD@MFs were obtained. A study on HeLa and A549 cancer cells showed that the inhibition rates of RGD@HPMFs were higher than that of RGD@MFs by 14.1% and 6.9%, respectively. The results obtained herein show that HPMF scaffold preparation by improving the material groups and regulating the structure of MFs can address the challenges associated with control of the load and sustained release of polypeptides and other drugs.
研究领域:
生物医学工程
·
材料科学
·
药物递送系统
✓ 已验证
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2023
·
IF 6.6
Yue Wu,Xiaotian Zhang,Mengyao He,Xue Tian,Neng Qian,Yangyi Sun,Dongming Qi
Microfiber-composed functional synthetic leather is of particular interest because it owns the advantages of good fluffiness, softness, excellent hygroscopicity , the random three-dimensional bundle structure similar to that of natural leather, which grants excellent moisture permeability, air permeability , chemical resistance, waterproof and mildew resistance, and various functions. Typically, the microfiber of functional synthetic leather is obtained through splitting the original sea-island fiber by solvent-induced dissolution and etching of its sea phase, which inevitably leads to the decline of the wettability of the treated leather. Herein, light-triggered functional microcapsules for self-healing wettability of split microfibers have been judiciously designed and developed. These functional microcapsules loaded with fluoroalkyl silane , which are used to coat onto the original sea-island fiber, allow for photocontrol of releasing the superhydrophobic chemical to the coated fibers. Despite the superhydrophobicity of the split fiber would be weakened by the splitting operation, the light-triggered release of fluoroalkyl silane can effectively amend its superhydrophobic properties. The microfiber leather self-repaired by the functional microcapsules exhibits good superhydrophobic performance, excellent self-cleaning ability to dust and RhB solution, high friction resistance (up to 200 rubs), and good UV light self-repairing cycle performance. This study provides a versatile solution for efficiently self-repairing microfiber leathers after the splitting process.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Biopreservation and Biobanking
·
2020
·Vol. 18
·期 3
·228-234
·
IF 2.022
Kexin Cao,Lingxiao Shen,Xiaojie Guo,Kun Wang,Xiaoyu Hu,Xilin Ouyang,Gang Zhao
Cryopreservation of red blood cells (RBCs) has been studied as a typical example of cryobiology methodology. To date, a mature and long-term cryopreservation process for RBCs has been developed, which has the weakness of complicated procedures due to high concentrations of glycerol (Gly). Therefore, it is still a research focus to find a new method for cryopreservation of RBCs to reduce the concentrations of cryoprotectants (CPAs). In this study, alginate hydrogels, which have been widely used in preservation research, were selected because of their advantages such as lower cost, cytocompatibility, and crosslinking occurring under mild conditions. With a variety of CPA solutions, the RBC recovery with the cryopreservation of RBCs and RBC-laden hydrogel microfibers was compared in different situations. It was found that hydrogel microfiber encapsulation enhances cryopreservation of human RBCs with low concentrations of CPAs. And Gly can be removed by washing directly with a 0.9% sodium chloride solution. This study validated the feasibility of cryopreservation of RBC-laden hydrogel microfibers. It may provide a new and evolving direction for reducing the concentrations of CPAs used in the preservation of RBCs.
研究领域:
生物医学工程
·
低温生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）