Journal of Chromatography Open
·
2022
·
IF 0
Anting Wang,Yuanhang Wang,Qin Huang,Xirenguli Hailiwuli,Lei Zhao,Zhaoyan Wang,Qiaosheng Pu
Capillary electrophoresis (CE) is a powerful separation tool for multicomponent analysis, but the necessity of switching the inlet end of the capillary between the vials of sample and running buffer for the sample injection complicates the operation and contributes to the irreproducibility, leading to poor compatibility with on-line analysis. Herein, coupling of capillaries with the aid of a plastic microchip was achieved for consecutive analysis. An assembly of 3 pieces of capillary with a cyclic olefin copolymer (COC) microchip was fabricated by thermal bonding of the COC plates with capillaries embedded in the micro-grooves with a cross-type configuration. Two capillaries acted as a sampling channel and the third capillary was used for separation. A buffer reservoir was installed on the microchip, which connected to the joint of the capillaries. The relative liquid levels of this reservoir and the vials at the other ends of the capillaries determine the length of the sample plug. With this assembly, the RSD of peak height and peak area for consecutive analysis were 0.54 % and 2.27 % (n = 9), the sample carryover was less than 0.32 %. The applicability of the device was confirmed by monitoring the leaching process of K + in a green tea and vitamin C effervescent tablets.
研究领域:
分析化学
·
生物医学工程
·
微流控技术
✓ 已验证
DOI
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2016
·
IF 0.566
Da-Peng GUO,Yong-Huan WANG,Tian-Bai XU,Yuan ZHANG,Ling-Feng Li,Jin-Kai CHEN,Xiao-Zhi WANG,Peng LI,Ji-Kui LUO
The microchip field asymmetric ion mobility spectrometry (FAIMS) technology was used for rapid analysis of phenylalanine (PHE) using the metal diffusion tube-microchip μ-FAIMS system. The test pressure was set at 0.25 MPa, and the temperature of the gas generator for the diffusion tube was at 190 °C. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL min −1 , a dispersion voltage of 152.8 V and a compensation voltage of −0.62 V for phenylalanine in positive mode. Phenylalanine gas samples with different concentrations were analyzed in detail by FAIMS. The results showed that the ion intensity was linearly correlated with phenylalanine concentration in the range from 6 mg L −1 to 20 mg L −1 , with the detection limit of 5.93 mg L −1 for phenylalanine. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple, and has high sensitivity and fast response, and shows great potential for rapid detection of phenylalanine.
研究领域:
分析化学
·
医学检验
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Li-Li Xu,Ming Wang,Yi-Ke Wang,Yi-Jing Chen,Yu-Xin Zhang,Yan-Qiu Zhang,Shi-Bo Cheng,Min Xie,Wei-Hua Huang
Microchip-based exosome analysis has emerged as a promising approach for liquid biopsy in cancer diagnosis, treatment monitoring, and prognostic evaluation. However, current microchips for exosome analysis typically rely on planar, 2D channel structures with affinity properties, which require complex fabrication but deliver suboptimal separation and detection performance. This study presents a novel vessel-like microtunnel chip, integrated with biomimetic octopus tentacles, achieving an exosome isolation efficiency of 90.4%. The innovative design incorporates interwoven, 3D micropathways, enhancing fluid dynamics and promoting efficient mixing between exosomes and microchannels. Nanofiber-coated silicon microspheres, functionalized with synthetic peptides, mimic octopus tentacles to anchor the microtunnels, dynamically extending under fluid shear forces to specifically recognize lipid bilayer structures for exosome capture. This platform incorporates enzyme-catalyzed signal amplification using Au nanoprobes for colorimetric detection to sensitively analyze four protein markers on plasma-derived exosomes from 60 clinical samples. Machine learning is used to develop a diagnostic model, achieving an area under the curve (AUC) of 0.9888 in distinguishing pancreatic cancer from pancreatitis and healthy controls. This approach provides a rapid, sensitive, accurate, and user-friendly method for pancreatic cancer diagnosis, addressing the clinical challenges of early detection and the frequent misdiagnosis of pancreatic cancer as pancreatitis.
研究领域:
医学
·
生物工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2013
·
IF 2.591
Xinchun Li,Zuanguang Chen,Fan Yang,Jianbin Pan,Yinbao Li
L -3,4-dihydroxyphenylalanine (L-DOPA) is a well-recognized therapeutic compound to Parkinson's disease. Tyrosine is a precursor for the biosynthesis of L-DOPA, both of which are widely found in traditional medicinal material, Mucuna pruriens . In this paper, we described a validated novel analytical method based on microchip capillary electrophoresis with pulsed electrochemical detection for the simultaneous measurement of L-DOPA and tyrosine in M . pruriens . This protocol adopted end-channel amperometric detection using platinum disk electrode on a homemade glass/polydimethylsiloxane electrophoresis microchip. The background buffer consisted of 10 mM borate (pH 9.5) and 0.02 mM cetyltrimethylammonium bromide, which can produce an effective resolution for the two analytes. In the optimal condition, sufficient electrophoretic separation and sensitive detection for the target analytes can be realized within 60 s. Both tyrosine and L-DOPA yielded linear response in the concentration range of 5.0–400 μM ( R 2 > 0.99), and the LOD were 0.79 and 1.1 μM, respectively. The accuracy and precision of the established method were favorable. The present method shows several merits such as facile apparatus, high speed, low cost and minimal pollution, and provides a means for the pharmacologically active ingredients assay in M . pruriens .
研究领域:
分析化学
·
药学
·
植物化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Zhenping Li,Hao Xu,Yukun Du,Zhongyu Huang,Jing Li,Lian Liu,Ying Dong,Xiao-Yu Yang
Gamma-hydroxybutyric acid (GHB), which is used in drug-facilitated sexual assault, is typically delivered in the form of gamma-butyrolactone (GBL) in beverage carriers. In response to the growing need of methods for rapid and on-site sensing of the drugs, we carried out an investigation aimed at developing a superwettable microchip for visual detection of the date-rape drug GBL in adulterated beverages. The superwettable microchip is comprised of Fe 3 O 4 nanoparticles which aggregate to form a reactive spot on a superhydrophobic fabric. The spot captures and confines microdroplets containing GBL on the superhydrophobic substrate. Fe(III), generated by HCl promoted reaction of the Fe 3 O 4 spot, then participates in a colorimetric reaction with GBL to form a naked eye observable colored complex. Quantitative determination of GBL in beverages is achieved by analyzing the red proportion of a smartphone camera derived image. The detection range of this assay protocol is ca. 0.1–10 mg mL −1 , which meets the requirements to detect potentially dangerous concentration of GBL in drinks. The detection stability of the superwettable microchip for GBL in water and common beverages is maintained over at least 30 cycles.
研究领域:
分析化学
·
法医学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Qidan Cai,Huaidong Peng,Jiang Meng,Youshao Yan,Yu Zeng,Paul C.H. Li,Yue Sun
In this study, a three-phase laminar flow microfluidic chip (TPL chip) combined with HPLC was developed for monitoring free and total concentrations of paclitaxel (PTX) in blood simultaneously. A diluted whole blood sample (aqueous phase) was introduced into the chip, ethyl acetate (organic phase) was introduced into the chip for extraction, and an interphase was used to prevent the blood sample from coming into direct contact with the organic phase. Because only free drug can quantitatively diffuse into the organic extraction phase and the free drug fraction has a linear relationship with the dilution factor of blood, both the free and total drug concentrations can be obtained by detecting the concentration of paclitaxel in the organic extraction phase. The governing factor such as flow rate for extraction was optimized. Docetaxel was used as an internal standard. The reliability of the quantitative diffusion of molecules in the TPL chip was proved by the methodological investigation of PTX in PBS sample, which showed a good linearity in the concentration range of 0.5 - 100 µg/mL and a detection limit of 7 ng/mL. Good repeatibilities for retention time (RSD of PTX is 1.23%, docetaxel is 1.14%, n = 5) and peak area ratio of PTX to docetaxel (RSD is 4.38%) were obtained. For blood sample analysis, only 100 µL of sample was needed and whole pretreatment was finished in 35 min, and a recovery of 94~117% were obtained. The provided method showed advantages in fast analysis speed, minimum sample handing, and potential ability of automation, and integration.
研究领域:
分析化学
·
药代动力学
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2013
·
IF 3.925
Ruina Li,Lili Wang,Xiaotong Gao,Gangfeng Du,Honglin Zhai,Xiayan Wang,Guangsheng Guo,Qiaosheng Pu
Rapid analysis of trace amount of aromatic amines in environmental samples and daily necessities has attracted considerable attentions because some of them are strongly toxic and carcinogenic. In this study, fast and efficient electrophoretic separation and sensitive determination of 5 banned aromatic amines were explored for practical analysis using disposable plastic microchips combined with a low-cost laser-induced fluorescence detector. The effect of running buffer and its additive was systematically investigated. Under the selected condition, 5 fluorescein isothiocyanate labeled aromatic amines could be baseline separated within 90 s by using a 10 mmol/L borate buffer containing 2% (w/v) hydroxypropyl cellulose. Calibration curves of peak areas vs. concentrations were linear up to 40 or 120 μmol/L for different analytes and limits of detection were in a range of 1–3 nmol/L. Theoretical plate numbers of 6.8–8.5 × 10 5 /m were readily achieved. The method exhibited good repeatability, relative standard deviations ( n = 5) of peak areas and migration times were no more than 4.6% and 0.9%, respectively. The established method was successfully applied in the quantitative analysis of these banned aromatic amines in real samples of waste water and textile, recoveries of added standards were 85–110%.
研究领域:
分析化学
·
环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Haifeng Yang,Zhiqiang Qiao,Weimiao Wang,Pengfei Tang,Shuaishuai Man,Xiaodong Li,YuTing Xie,Deyun Tang,Xueming Li,Guangcheng Yang
Private information protection of electronic chips which store large amounts of sensitive data, is a crucial issue in electromechanical systems nowadays. Here, a self-destructive microchip based on an instantaneous thermite reaction is designed to prevent the leakage of electronic information. The device is consisted of a silicon test wafer and an upper energetic film coated by a simple drop-casting technique. A series of Al/BiOX-based nanothermites have been prepared for decreasing the reaction sensitivity, meanwhile optimizing the destruction efficiency of the energetic film system. Among them, Al/Bi 2 O 3 nanothermite containing 30 wt% BiOBr possesses a low sensitivity (45 J), the maximum pressure peak (1.93 MPa) and pressure effect duration due to multiple interactions of the three nano-scale energetic components. Besides, the energetic film prepared by this nanothermite fomula also exhibits a more effective destruction of the device. The high-speed photography results demonstrate that the violent reaction shatters the thick energetic microchip (∼1 mm) within merely 0.5 ms. Importantly, the efficient destruction of energetic microchips shows excellent reproducibility in different working scenes, including Air or Ar atmosphere. It is believed that this self-destructive device with environmentally friendly components and simple but adaptive design can be extensively used in various electric microcomponents.
研究领域:
材料科学
·
电子工程
·
安全技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 21
·e70013
·
IF 2.8
Zhilei Li,Gangyuan Lin,Xiujuan Yang
A novel and rapid method was developed for the determination of galantamine in human plasma by microchip electrophoresis with a highly integrated contactless conductivity detector (CCD). The instrumental parameters affecting the response of the detector, such as excitation frequency and excitation voltage, were examined and optimized. The electrophoresis conditions that influenced the separation and detection of galantamine, including the composition of buffer solution, buffer pH, buffer concentration, additives, injection time, and separation voltage were systematically investigated. Under the optimal conditions, the peak height had a good linear relationship with the concentration of galantamine in human plasma from 10 to 160 µg/L, and the correlation coefficient was 0.9992, the limit of detection reached 1.1 µg/L. The recoveries were between 98.6% and 102.1%. This sensitive, rapid, and convenient method is a good alternative to existing methods for galantamine determination. Also, this highly integrated CCD holds great promise in clinical biochemical analysis.
研究领域:
分析化学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2012
·Vol. 33
·期 16
·2591-2597
·
IF 3.303
Li Li,Xiaodong Bi,Jianzhao Yu,Carolyn L. Ren,Zhen Liu
Manufacturing materials are an essential element for the fabrication of microfluidic chips. PDMS, the most widely used polymeric material, is associated with apparent disadvantages such as hydrophobic nature, while other materials also suffer from some limitations. In this paper, a new soft lithographic route was proposed for the facile manufacturing of hydrophilic sandwich microchips, using bisphenol A based epoxy acrylate (BABEA) as a new patterning material. The BABEA copolymers are hydrophilic, highly transparent in visible range while highly untransparent when the wavelength is less than 290 nm, and of high replication fidelity. By combining with appropriate monomers, including glycidyl methacrylate, methylmethacrylate, and acrylic acid, the copolymers contain active functional groups, which allows for easy postmodification for desirable functional units. A fabrication procedure was proposed for manufacturing hybrid quartz/BABEA copolymer/quartz microchips. In the procedure, no micromachining equipments, wet etching, or imprinting techniques were involved, making the fabrication approach applicable in ordinary chemistry laboratories. The performance of the prepared microchips was demonstrated in terms of CIEF with UV-whole channel imaging detection. The hydrophilic microchannel ensures stable focusing while the polymeric middle layer acts as a perfectly aligned optical slit for whole channel UV absorbance detection.
研究领域:
微流控技术
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Xuan Wei,Ping Sun,Shenghong Yang,Lei Zhao,Jing Wu,Fengyun Li,Qiaosheng Pu
Plastic microchips can significantly reduce the fabrication cost but the adsorption of some analytes limits their application. In this work, background electrolyte containing ionic polymer and high content of organic solvent was adopted to eliminate the analyte adsorption and achieve highly efficient separation in microchip electrophoresis. Two dyes, rhodamine 6G (Rh6G) and rhodamine B (RhB) were used as the model analytes. By using methanol as the organic solvent and polyacrylic acid (PAA) as a multifunctional additive, successful separation of the two dyes within 75 μm id. microchannels was realized. The role of PAA is multiple, including viscosity regulator, selectivity modifier and active additive for counteracting analyte adsorption on the microchannel surface. The number of theoretical plate of 7.0 × 10 5 /m was attained within an effective separation distance of 2 cm using background electrolyte consisting 80% methanol, 0.36% PAA and 30 mmol/L phosphate at pH 5.0. Under optimized conditions, relative standard deviations of Rh6G and RhB detection (n = 5) were no more than 1.5% for migration time and 2.0% for peak area, respectively. The limit of detection (S/N = 3) was 0.1 nmol/L for Rh6G. The proposed technique was applied in the determination of both Rh6G and RhB in chilli powder and lipstick samples with satisfactory recoveries of 81.3–103.7%.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2011
·Vol. 175
·期 1
·193-199
·
IF 2.578
Yang Hui,Li Xin-chun,Yang Fan,Feng Jun,Lin Miao-yin,Chen Zuan-guang
We describe an efficient and easily fabricated electrochemiluminescence detection system for microchip capillary electrophoresis. A 300-μm-diameter platinum disc working electrode was embedded in a titanium tube which provides an adequate holding for working electrode and acts as counter electrode. We also have designed a simplified detection cell with a guide channel for the electrode. The integrated working-counter electrode can be easily aligned to the outlet of the separation channel through the guide channel. The functionality of the system was demonstrated by separation and detection of proline and tripropylamine. The response to proline is linear in the range from 5 μM to 5,000 μM, and the detection limit is 1.0 μM (S/N = 3). The system was further applied to the determination of chlorpromazine hydrochloride in pharmaceutical formulations. The system is believed to have potential applications in pharmaceutical analysis.
We described an efficient and easily fabricated electrochemiluminescence detection system for microchip capillary electrophoresis. The functionality of the system was demonstrated by separation and detection of proline and tripropylamine. The response to proline is linear in the range from 5 μM to 5,000 μM, and the detection limit is 1.0 μM (S/N = 3).
研究领域:
分析化学
·
制药工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）