Polymers
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2022
·Vol. 14
·期 19
·3941
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IF 4.967
Xu Gao,Jun-Feng Su,Sai Wang,Peng Yang
Artificial skin composites have attracted great interest in functional composite materials. The aim of this study was to prepare and characterize a smart artificial skin composite comprising a bionic microvascular with both self-nourishing and self-healing functions. A poly(vinyl alcohol)–glycerol–gelatin double network organic hydrogel was used as the artificial skin matrix. The hydrogel had high mechanical strength because of the strong hydrogen bond formed between the PVA and glycerol (GL). The gelatin (GEL) increased the toughness and elasticity of the hydrogel to ensure the strength of the artificial skin and fit of the interface with the body. The bionic polyvinylidene fluoride (PVDF) microvascular had excellent thermal stability and mechanical property in artificial skin. Results indicated that self-nourishing was successfully realized by liquid release through the pore structures of the bionic microvascular. The bionic microvascular healed microcracks in the artificial skin when damage occurred, based on a self-healing test.Keywords:artificial skin;self-nourishing;self-healing;microvascular
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Bingjie Li,Jinbin Pan,Ruijie Zhang,Bing Han,Yujie Zhao,Guijun Liu,Yujie Tong,Yujing He,Guangchao Xie,Ruxia Liu,Ting Zhou,Quan Zhang,Shao-Kai Sun
High-resolution vascular imaging at tens of micrometers in deep tissues in vivo remains a critical challenge. Ultrahigh field susceptibility-weighted imaging (SWI) holds promise but lacking compatible high-sensitivity imaging probes. Herein, we show a holmium (Ho)-based nanoprobes-enhanced SWI strategy for ultrahigh-resolution imaging of cerebral microvessels at 9.4 T. The polyethylene glycol (PEG)-NaHoF 4 nanoprobes (NPs) fabricated via coprecipitation synthesis combined with PEG modification possess uniform size, appropriate hydrodynamic size (20 nm), good biocompatibility, and long circulation half-life (710 min). Notably, the PEG-NaHoF 4 NPs exhibit high r 2 / r 1 742.7) and T 2 * relaxivity ( r 2 *, 73.16 s -1 mM -1 ) under 9.4 T due to the large magnetic moment (∼10.6 μ B ) and short electronic relaxation time (∼10 -13 s) of Ho 3+ . The high susceptibility of PEG-NaHoF 4 NPs in blood vessels induces a significant blooming effect, resulting in a magnified vascular appearance on SWI. In vivo high-resolution imaging of cerebral microvessels with diameters as small as 10 μm is achieved using PEG-NaHoF 4 NPs-enhanced SWI under 9.4 T. In two representative brain disease models, glioma and stroke, this nanoprobe enables high-resolution visualization of tumor vasculature and post-stroke collateral circulation, respectively. Our study offers a new paradigm for precise diagnosis of vascular-related diseases, providing a robust tool for their diagnosis, treatment, and prognosis assessment.
研究领域:
医学影像学
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神经科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hui Su,Yucheng Qiu,Jing Luo,Fei Liu,Jun Yang,Xiaofeng Sui,Yue Zhang,Yumei Zhang,Xianyu Zhou
Vascular anastomosis is the cornerstone in microsurgery. To assist complicated microvascular anastomosis with diameters <0.6 mm, nonsuture cuff technique was developed. However, cuff tube made from polyamide is not degradable, and their long-term retention in the body may cause stiffness in blood vessels at the anastomotic site. Thus, development of a biodegradable cuff tube would greatly enhance surgical operability and biosafety. In this study, biocompatible poly-L-lactic acid (PLLA) was chosen as the raw material to prepare cuff tube for nonsuture cuff technique. A customized ‘C’ shaped nozzle was adopted during the melting extruding process, and then was subjected to a water-cooling bath to maintain the round shape and inner diameter of 582 μm ± 20 μm. Its crystallization behavior was controlled through the cooling procedure to achieve the expected mechanical properties and operability. Its good hemocompatibility and genotoxicity were validated by various in vitro assays. Consequently, a PLLA cuff tube with a radial tensile strength of 12 MPa and an elastic modulus of 1685 MPa was prepared and used to assist establishing a rat orthotopic hindlimb transplantation model, which indicated that our PLLA cuff tube was suitable for microvascular anastomosis in organ or tissue repair.
研究领域:
医学
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生物材料学
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外科手术
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产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2024
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IF 6.5
Weichao Song,Xia Zhao,Zuquan Jin,Xiaohong Ji,Liang Fan,Shuai Yuan,Fubin Ma,Junying Deng,Jizhou Duan,Baorong Hou
Biomimetic microvascular networks prepared by coaxial electrospinning technology have attracted much attention as a novel form of carrier for encapsulating active substances. However, the poor interfacial bonding strength between traditional electrospinning materials and metal substrates limits its application in anti-corrosion coatings. Herein, a novel poly(vinyl alcohol) grafted phytic acid (PVA-PA) electrospinning solution was synthesized. And a sandwich-like microvascular network (SMN) was prepared by coaxial electrospinning technology, which used PVA-PA solution as the shell material, epoxy resin 51 (E51), tetraphenylethylene (TPE) and polyamide resin as the core materials, respectively. Owing to the high porosity of SMN, epoxy resin can be directly spin-coated on it to form a composite coating (PVA-PA/SMN/EP). It is proved that due to the strong chelation and coordination interaction between PA and mild steel, the pull-out adhesion of the PVA-PA/SMN/EP composite coatings on mild steel was increased by 0.92 MPa. In addition, by systematically optimizing the relative viscosity, miscibility, conductivity, and saturated vapor pressure between the two jets of the core solution and the shell solution in coaxial electrospinning, the microvascular structure of the coaxial electrospinning nanofibers was improved and the fluidity of the internal active substances was maintained. When the PVA-PA/SMN/EP composite coating generates microcracks, the active substances encapsulated in the SMN flow out, in which the three-dimensional crosslinked network formed by the curing of E51 and polyamide resin enhances the spatial interactions between TPE molecules, which results in TPE emitting a bright blue fluorescence. This work provides a new approach for the development of the next generation of smart anti-corrosion coatings.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
BIOMEDICAL MICRODEVICES
·
2017
·Vol. 19
·期 3
·1-8
·
IF 2.062
Li Yuan,Lu Yao,Chen Qiong,Kang Yuejun,Yu Ling
Retinopathy is a complication of diabetes that affects the eyes; it stems from damage to the microvasculature of the retina and eventually compromises vision. The diagnosis of retinopathy is difficult to make because there are no early symptoms or warning signs. Dysfunction of the retina’s microvascular networks is believed to be associated with inflammatory cytokines and tumor necrosis factor alpha (TNF-α). To investigate the effect of these cytokines, such as TNF-α, a polydimethylsiloxane (PDMS)/glass hydride microfluidic device reflecting the physiological structure of the retina’s microvasculature was developed. In this model, the bifurcations and tortuosity of branch vessels were based on photographs of the fundus and an endothelial cell layer (EA.hy926 cells) were reconstructed within the microfluidic network. The adhesion, spreading, and growth of cells was ensured by optimizing the conditions for cell seeding and perfusion. Fluorescent staining was used to visualize the cytoskeleton and measurement of the nitric oxide (NO) level proved that the endothelial EA.hy926 cells had spread in the direction of flow perfusion system, forming artificial vascular networks. The endothelial layer was further challenged by TNF-α perfusion. Cytokine treatment increased the anchoring of peripheral blood mononuclear cells (PBMCs) on the endothelial layer. The microfluidic device developed in this study provides a low-cost platform reflecting the physiological structures of the retina’s microvasculature. It is anticipated that this device will be useful in evaluating the diseased retina as well as in drug screening.
研究领域:
医学
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生物工程
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
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IF 3.671
Han Yue,Yanqiu Liu,Huanhuan Qu,Kan Ding
Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on. Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated. In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide. It composed of arabinose, xylose, glucose and 4- O -methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7. We further showed that it contained a backbone of 1,4-linked β- d -xylan, with branches of 1,4-linked α- d -glucose, 1,3-linked α- l -rhamnose, and terminal-linked α- l -arabinose, β- d -galactose, 4-MGA, and β- d -xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone. The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method. Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29 μM) in a dose-dependent way without significant cytotoxity.
研究领域:
植物化学
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药理学
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DOI
PubMed
高影响因子
Annals of Translational Medicine
·
2021
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IF 3.932
Xiangrui Meng,Yuanyuan Sun,Lan Wang,Yuhao Li,Ruizhuo Ouyang,Ping Yuan,Yuqing Miao
Background Under hypoxic conditions, the brain can undergo irreversible damage. The present study aimed to explore new higher-oxygen-content carbamide peroxide (CP) compounds and the effect of their oxygen-releasing property on human brain microvascular endothelial cell (EC) proliferation under in vitro hypoxic conditions. Methods Two different additives including alpha-terpineol and sorbic acid were added to the reaction system to obtain the carbamide peroxide of CP-I and CP-II. Results We evaluated the oxygen generation capabilities of CP samples by using a portable dissolved oxygen meter. Consequently, alpha-terpineol as a stabilizer exhibited a unique effect on the oxygen generation of CP. CP-I was uniquely able to promote cell proliferation ability at 10 µg·L −1 for hypoxic conditions, with the proliferation rates being 36.2% compared with the control group. The safety of CP to cells was further verified by calcein-AM/PI staining. Under hypoxic conditions, CP-I at 10 µg·L −1 promoted the migration rate, and the migration rate being 32.37%. Conclusions These compounds have the advantages of simple synthesis, long storage time, low cost, and rich oxygen content. Used spectrophotometry, oxygen electrode test, and indicator titration for testing the oxygen production rate and oxygen production. The results indicate that alpha-terpineol is the best additive. CP-I exhibited the highest oxygen content and a superior effect on the cell phenotype than CP-II, especially under hypoxia. This study is the first to report the effects of CP on cells, and provides new therapeutic insights into cerebrovascular injury repair.
研究领域:
神经生物学
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细胞生物学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-16
·
IF 10.6
Li Chao,Xiang Zhenyang,Hou Mengfan,Yu Hao,Peng Peng,Lv Yigang,Ma Chao,Ding Han,Jiang Yunpeng,Liu Yang,Zhou Hengxing,Feng Shiqing
Background Spinal cord injury (SCI) often leads to a loss of motor and sensory function. Axon regeneration and outgrowth are key events for functional recovery after spinal cord injury. Endogenous growth of axons is associated with a variety of factors. Inspired by the relationship between developing nerves and blood vessels, we believe spinal cord-derived microvascular endothelial cells (SCMECs) play an important role in axon growth. Results We found SCMECs could promote axon growth when co-cultured with neurons in direct and indirect co-culture systems via downregulating the miR-323-5p expression of neurons. In rats with spinal cord injury, neuron-targeting nanoparticles were employed to regulate miR-323-5p expression in residual neurons and promote function recovery. Conclusions Our study suggests that SCMEC can promote axon outgrowth by downregulating miR-323-5p expression within neurons, and miR-323-5p could be selected as a potential target for spinal cord injury repair. Graphical Abstract
研究领域:
神经科学
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分子生物学
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再生医学
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DOI
PubMed
高影响因子
Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1-19
·
IF 7.5
Han Ning,Yu Na,Yu Li
Background Diabetic retinopathy (DR) is the common cause of diabetic vascular complications and it causes blindness. Until now, there are still some patients with DR who lack effective treatment. Tripartite motif containing 44 (TRIM44) has been shown to play a significant role in endothelial cells. However, the role of TRIM44 in DR remains unknown. Methods Diabetes was induced in rats through the administration of an intraperitoneal injection of 65 mg/kg of streptozotocin (STZ). Rat retinal microvascular endothelial cells (RMECs) were subjected to stimulation under high glucose (HG) conditions. A thorough proteomic investigation and bioinformatic analysis were performed to identify the differentially expressed proteins (DEPs) in rat RMECs after blocking TRIM44. A dual luciferase reporter assay was employed to assess the luciferase activity of TRIM44. Results TRIM44 was highly expressed in the retinal tissues of rats with diabetes and HG-induced RMECs. In vivo assays suggested that TRIM44 silencing improved the pathological alterations of DR rats as demonstrated by the downregulated expression of isolectin-B4 and VEGFA, along with a decrease in acellular capillaries within the retinal tissues. Knockdown of TRIM44 markedly reduced cell viability, proliferation, migration, invasion, and angiogenesis in HG-evoked RMECs. Mechanistically, TRIM44 was demonstrated to be activated transcriptionally by KLF transcription factor 9 (KLF9), a known facilitator of angiogenesis in DR. In HG-induced cells, the loss of TRIM44 resulted in the reverse of the endothelial cell function caused by KLF9 overexpression. After the comprehensive analysis, 64 upregulated and 38 downregulated DEPs were screened out for a series of functional enrichment analyses. Conclusions Collectively, this study demonstrates that TRIM44 knockdown suppressed diabetes-induced retinal vascular dysfunction in DR.
研究领域:
分子生物学
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糖尿病学
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眼科学
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产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Yuzhi Guo,Ziyu Wang,Xuening Zhang,Jinghang Li,Shan Gao,Yang Lv,Liliang Ouyang
Vascularization is key to the biofabrication of large-scale tissues. Despite the progress, there remain some outstanding challenges, such as limited vessel density, difficulty in fabricating microvasculatures, and inhomogeneity of post-seeding cells. Here, a new form of bioink called microfiber-templated porogel (µFTP) bioink is introduced to engineer vasculatures down to the filament building blocks of 3D bioprinted hydrogels. The cell-laden sacrificial microfibers (diameter ranges from 50–150 µm) are embedded in the bioink to template tubular voids and deliver endothelial cells for in-situ endothelialization. The inclusion of softening hydrogel microfibers retains the desirable rheological properties of the bioink for extrusion-based bioprinting and the microfibers are well inter-contacted in the extruded filament. Such bioinks can be printed into a well-defined 3D structure with tunable tubular porosities up to 55%. Compared to the conventional bulk bioink counterpart, the µFTP bioink supports the significant growth and spread of endothelial cells either embedded in the matrix or sacrificial fibers, free of the post-cell seeding procedure. Furthermore, the bioprinted scaffolds based on µFTP bioink are seen to significantly promote the in-growth of blood vessels and native tissues in vivo. The µFTP bioink approach enables the engineering of tubular bio-interfaces within the building blocks and contributes to the in-situ endothelialization of microvasculatures, providing a versatile tool for the construction of customized vascularized tissue models.
研究领域:
生物医学工程
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yawei Zhao,Jing Zhang,Xiaohan Fan,Yue Cai,Wenting Du,Ziyi Yu,Biao Xu,Xuan Sun
Microvascular obstruction (MVO) is recognized as an independent risk factor for adverse prognosis in patients with myocardial infarction (MI), and its development is mechanistically linked to inflammation and microthrombus. Maresin 1 (MaR1), a specialized pro-resolving mediator, has demonstrated therapeutic potential in counteracting these processes, but its translation has been hindered by poor stability and rapid degradation. To overcome these limitations, we report the development of a cardiac-targeted liposomal nanotherapeutic (nano-MaR1) designed to stabilize and selectively deliver MaR1 to the injured myocardium. Nano-MaR1 was generated through a scalable nanoprecipitation method in a herringbone microfluidic mixer, with MaR1 encapsulated by a freeze–thaw approach to minimize degradation, and further modified with a cardiac-homing peptide for organ-specific targeting. In a murine MI model, intravenous administration of nano-MaR1 led to rapid myocardial enrichment within 12 h, with 20 μM identified as the optimal therapeutic dose. Treatment markedly reduced infarct size, cardiomyocyte apoptosis, inflammatory infiltration, and MVO formation, while promoting reparative macrophage polarization toward the M2 phenotype. Mechanistic studies revealed that these effects were mediated by downregulation of Itgam (CD11b) within the coagulation cascade and inhibition of monocyte–platelet aggregate formation, which were abolished upon CD11b blockade. Collectively, this work establishes cardiac-targeted liposomal encapsulation as a powerful strategy to enhance the stability and bioactivity of MaR1, and highlights nano-MaR1 as a promising therapeutic candidate for mitigating MVO and improving post-MI repair.
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产品关联
DOI
高影响因子
Cell Reports
·
2023
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IF 8.8
Liang Zhu,Mengqi Wang,Yin Liu,Peng Fu,Weijie Zhang,Hequn Zhang,Anna Wang Roe,Wang Xi
Recent studies have highlighted the importance of understanding the architecture and function of microvasculature, and dysfunction of these microvessels may underlie neurodegenerative disease. Here, we utilize a high-precision ultrafast laser-induced photothrombosis (PLP) method to occlude single capillaries and then quantitatively study the effects on vasodynamics and surrounding neurons. Analysis of the microvascular architecture and hemodynamics after single-capillary occlusion reveals distinct changes upstream vs. downstream branches, which shows rapid regional flow redistribution and local downstream blood-brain barrier (BBB) leakage. Focal ischemia via capillary occlusions surrounding labeled target neurons induces dramatic and rapid lamina-specific changes in neuronal dendritic architecture. Further, we find that micro-occlusion at two different depths within the same vascular arbor results in distinct effects on flow profiles in layers 2/3 vs layer 4. The current results reveal laminar-scale regulation distinctions in microinfarct response and raise the possibility that relatively greater impacts on microvascular function contribute to cognitive decline in neurodegenerative disease.
研究领域:
神经科学
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医学影像学
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病理学
✓ 已验证
DOI
PubMed
高影响因子
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