BIOMEDICAL MICRODEVICES
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2019
·Vol. 21
·期 1
·1-13
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IF 2.327
Shen Yiren,Du Kun,Zou Lili,Zhou Xiaoming,Lv Rong,Gao Dayong,Qiu Bensheng,Ding Weiping
Freeze-drying is a promising approach for the long-term storage of erythrocytes at room temperature. Studies have shown that trehalose loaded into erythrocytes plays an important role in protecting erythrocytes against freeze-drying damage. Due to the impermeability of the erythrocyte membrane to trehalose, many methods have been developed to load trehalose into erythrocytes. However, these methods usually require multistep manual manipulation and long processing time; the adopted protocols are also diverse and not standardized. Thus, we develop an osmotically-based trehalose-loading microdevice (TLM) to rapidly, continuously, and automatically produce erythrocytes with loaded trehalose. In the TLM, trehalose is loaded through the erythrocyte membrane pores induced by hypotonic shock; then, the trehalose-loaded erythrocytes are rinsed to remove hemoglobin molecules and cell fragments, and the extracellular solution is restored to the isotonic state by integrating a rinsing-recovering design. First, the mixing function and the rinsing-recovering function were confirmed using a fluorescent solution. Then, the performance of the TLM was evaluated under various operating conditions with respect to the loading efficiency of trehalose, the hemolysis rate of erythrocytes ( ϕ ), the recovery rate of hemoglobin in erythrocytes ( φ ), and the separation efficiency of the TLM. Finally, the preliminary study of the freeze-drying of erythrocytes with loaded trehalose was accomplished using the TLM. The results showed that under the designated operating conditions, the loading efficiency for human erythrocytes reached ~21 mM in ~2 min with a ϕ value of ~17% and a φ value of ~74%. This study provides insights into the design of the on-chip loading of trehalose into erythrocytes and promotes the automation of life science studies on biochips.
研究领域:
生物技术
·
细胞生物学
·
微流控
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL MICRODEVICES
·
2017
·Vol. 19
·期 2
·1-14
·
IF 2.062
Zou Lili,Li Shibo,Kang Yufeng,Liu Juanjuan,He Liqun,Sun Sijie,Gao Dayong,Qiu Bensheng,Ding Weiping
In this study, we present a multistage-dialysis microdevice (MDM) for extraction of cryoprotectants (CPAs) from a CPA-laden cell suspension. We confirmed the functions of the key designs of the MDM using a fluorescence solution, we assessed the performance of the MDM by using the MDM to unload glycerin from glycerin-loaded swine erythrocytes, and we investigated the effects of the cell suspension flow rate, glycerin concentration, cell density, and membrane pore size on the clearance efficiency of glycerin (C G ), the survival rate of cells (S C ), and the recovery rate of cells (R C ). Under the designed conditions, C G , S C , and R C reached ~60%, ~90%, and ~70%, respectively. In addition, a high flow rate causes high S C and R C but a low C G . For a low glycerin concentration, C G , S C , and R C are all high. If a low cell density or a large pore membrane is used, C G is high, whereas both S C and R C are low. This work provides insight into the development of microfluidic devices for the inline extraction of cryoprotectants from a small volume of cryopreserved cells prior to the use of the cells in lab-on-a-chip applications.
研究领域:
生物医学工程
·
微流控技术
·
低温生物学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jianguo Chang,Nailong Gao,Peng Dai,Ziming Zhu,Hui You,Wei Han,Lu Li
The powerful immunoassay has attracted increasing attention for detecting trace substances. Herein, based on the co-coupling of phenylboronic acid and Protein A for oriented antibodies immobilization, we proposed a polymeric device-based fluorescence immunoassay with enhanced assay performance for Alpha-fetoprotein (AFP) analysis. Unlike traditional physical absorption or chemical covalent conjugation to immobilize antibodies, in this immunoassay, the bioreactivity of AFP antibody was maximumly obtained owing to the oriented immobilization manner. The package density of AFP antibody on the polymethylmethacrylate (PMMA) surface is significantly improved because of the co-coupling case associated distance effect. In addition, the non-specific absorption is greatly inhibited with the combined using of zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) and bovine serum albumin (BSA). These integrated features facilitate the output of strong fluorescence in the presence of AFP but emit very weak background in the absence of AFP. After a typical sandwich immunoreaction, the recorded fluorescence intensity of the immunosensor is proportional to the AFP concentration, showing excellent sensitivity, specificity, and reliability for AFP determination. Comparative studies show the co-coupling is superior than its single coupling counterparts. We envision this unique immunoassay can provide a new avenue for building immunosensors in the fields of disease diagnosis, food safety detection, and environmental monitoring.
研究领域:
生物医学工程
·
分析化学
·
医学诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）