Yang Danyi,Lan Jingwei,Chen Jiao,Wu Yuyu,Qian Wen,Han Jiangbin
The aim of the present study was to develop a sensitive, high-throughput 96-well plate ultraviolet spectrometric method for the determination of peroxide value at trace levels in reagents and packaging materials. The analysis was carried out under acid-free conditions to minimize or prevent reactions between iodine molecules and oxygen in the air, thereby ensuring the accuracy and repeatability of the experiments. The wavelength was set at 360 nm. The analytical procedure was validated by evaluating linearity ( r 2 = 0.9998), precision (intra- and inter-day precision were less than 0.90 and 6.20%, respectively.), accuracy (100–110% of the spiked amount), and limits of detection ( LOD ) and quantification ( LOQ ). The LOD and LOQ were confirmed to be 20 and 50 ng/mL, respectively. This high-throughput technique reduces the risk of chemical changes during analysis due to time inconsistencies, which benefits the accuracy and reproducibility of results. The proposed method was then successfully applied to determine trace amounts of peroxides in ethyl acetate and packaging material samples. Overall, the present study offers an accurate and sensitive method for measuring trace-level peroxides.
Mingxuan Huang,Huafeng Wang,Shicong Gui,Chujia Xu,Yi Chen,Shaohua Hu,Yubo Li
Accurate monitoring of ROS levels is essential for oxidative stress (OS) management, but their low concentration and instability pose significant detection challenges. Here, we developed a highly sensitive and selective electrochemical biosensor for real-time, micro-volume ROS detection in leukemia cells. The sensor was fabricated using ultrathin g-C₃N₄-rGO (CN-rGO) nanosheet synthesized through a calcination and hydrothermal approach. These nanosheets were deposited onto a screen-printed carbon electrode (SPE) and further modified with silver nanoparticles (AgNPs) to construct a CN-rGO@AgNPs/SPE biosensor. The porous nanostructure significantly enhanced the catalytic surface area and electron transfer efficiency, achieving high sensitivities towards H₂O₂ and O₂·⁻ detection. Using a window titration platform, the sensor detected ROS in 0.1 mL lysates of leukemia cell lines (U-937, THP-1, and MOLM-13) with concentrations of 7.38 ± 4.81 μM, 11.74 ± 0.985 μM, 19.84 ± 3.73 μM, respectively. Furthermore, the platform allowed precise monitoring of doxorubicin (DOX)-induced OS, providing valuable insights for guiding leukemia chemotherapy. Additionally, Lipopolysaccharide (LPS) stimulation experiments demonstrated the sensor's exceptional real-time performance, enabling dynamic detection of H₂O₂ release kinetics and ROS generation rates at the cellular level. Combining high sensitivity, low detection limits, and micro-volume analysis capabilities, this sensor holds great potential for clinical auxiliary diagnosis and drug efficacy assessment in leukemia research.
危险品化学品经营许可证（带存储）