PHARMACEUTICAL BIOLOGY
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2013
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IF 1.206
Cheng Yang,Keng Wu,Shang-Hai Li,Qiong You
Context: The heart is one of the target organs susceptible to attack by sepsis, and protection of the cardiac function in sepsis or alleviation dysfunction caused by sepsis appears a serious and urgent problem.Objective: This study was designed to explore the effect of curcumin on myocardial injury induced by sepsis and to explore the therapeutic effect of curcumin in managing sepsis induced cardiac dysfunction.Methods: Cecal ligation and puncture surgery were used to establish the sepsis model. Curcumin was administered by peritoneal injection (200 mg/kg/d, 3 days). The effects of curcumin on the cardiac functions [Ejection Fraction (EF), Fractional Shortening (FS), Cardiac Output (CO), Heart Rate (HR)], body temperature, cTn I and superoxide dismutase levels, malondialdehyde content (an index of lipid peroxidation), and myocardial histopathological and ultrastructural studies were carried out.Results: We demonstrated that treatment of rats with curcumin significantly decreased elevated levels of cTn I and MDA (p < 0.05) in plasma, and increase the levels of SOD (p < 0.05) after CLP. Moreover, curcumin markedly enhanced the myocardial contractility by increasing the decreased EF and FS in rats with sepsis induced by CLP (p < 0.05). In addition, curcumin could alleviate the myocardial inflammation and structure damage of myocardial cells in sepsis induced by CLP.Conclusion: In conclusion, the results from the present study demonstrate that curcumin has the protective effects on cardiac function in rats with sepsis and curcumin could be considered as an effective and safe therapeutic agent for the management of sepsis induced cardiac dysfunction.
研究领域:
医学
·
药理学
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病理学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Qian Liu,Cheng Xu,Jing Jin,Wenxiang Li,Jingjia Liang,Shijie Zhou,Zhenkun Weng,Yong Zhou,Xudong Liao,Aihua Gu
Lead (Pb) is widely used in industrial and daily-use consumer products. Early-life exposure may increase the risk of lead-related heart problems in childhood. However, the effects of early-life lead exposure on fetal heart development and long-term cardiac outcomes are unknown. In this study, pregnant ICR mice were exposed to lead acetate trihydrate (50 mg/kg/d) via oral gavage from gestation day 1.5 until offspring weaning. Thereafter, the second hit model was established, two groups of offspring (4 weeks old) were either administered sterile saline or Angiotensin II (Ang II) for 4 weeks until euthanasia. We investigated lead-induced offspring heart damage from embryonic period to adulthood by echocardiographic analysis, pathological H&E staining, and ultrastructural examination, as well as mitochondrial function detection. The results showed early-life lead exposure predisposed offspring mice to decreased ejection fraction, increased left ventricular volume, accompanied by hypertrophy and dilation, cardiomyocyte sarcomere dysplasia, abnormal mitochondrial structure, mitochondrial dysfunction, and decreased expression of key sarcomeric and mitochondrial genes, rendering them more susceptible to cardiac hypertrophy, vascular wall thickening, cardiac fibrosis, apoptosis, and heart failure induced by Ang II infusion. This study elucidates early-life low dose lead exposure compromises cardiac development and exacerbates second hit-induced cardiac pathological responses in adulthood, which furnishes crucial scientific evidence pertaining to the cardiac toxicity and risk evaluation associated with early-life exposure to lead.
研究领域:
毒理学
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心血管医学
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发育生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Yueming Liang,Zhusheng Liu,Yuelan Pang,Min Li,Shengmeng Zheng,Fujing Pan,Chunyu Guo,Qianhua Wu,Ting Chen,Qiang Li,Zhonghua Liu
Long-term storage of Liupao tea is conducive to improving its flavour and commercial value. Although bacterial communities influence Liupao tea flavour, their impact during storage remains unclear. The aroma compounds and bacterial communities were determined by headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME-GC–MS) and Illumina Nova6000 analysis. A total of 18 differential flavour compounds were significantly altered with Liupao tea storage duration, particularly after 9 years of storage. Flavour compounds with sweet notes increased, while those associated with pungent note decreased. The composition and diversity of bacteria remarkable changed after 9 years of tea storage. Six core functional bacteria were identified as contributing to the flavour characteristics of Liupao tea during long-term storage. Our findings provide novel insights that bacteria drive the formation of flavour characteristics during Liupao tea storage duration, and Liupao tea stored for more than 9 years have the best flavour.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Jove-Journal of Visualized Experiments
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2021
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IF 1.355
Qixiao Jiang,Xiaohui Xu,Jamie C. DeWitt,Yuxin Zheng
Chicken embryos are a classical model in developmental studies. During the development of chicken embryos, the time window of heart development is well-defined, and it is relatively easy to achieve precise and timely exposure via multiple methods. Moreover, the process of heart development in chicken embryos is similar to mammals, also resulting in a four-chambered heart, making it a valuable alternative model in the assessment of developmental cardiotoxicities. In our lab, the chicken embryo model is routinely used in the assessment of developmental cardiotoxicities following exposure to various environmental pollutants, including per- and polyfluoroalkyl substances (PFAS), particulate matter (PMs), diesel exhaust (DE) and nano materials. The exposure time can be freely selected based on the need, from the beginning of development (embryonic day 0, ED0) all the way to the day prior to hatch. The major exposure methods include air-cell injection, direct microinjection, and air-cell inhalation (originally developed in our lab), and the currently available endpoints include cardiac function (electrocardiography), morphology (histological assessments) and molecular biological assessments (immunohistochemistry, qRT-PCR, western blotting, etc.). Of course, the chicken embryo model has its own limitations, such as limited availability of antibodies. Nevertheless, with more laboratories starting to utilize this model, it can be used to make significant contributions to the study of developmental cardiotoxicities.
研究领域:
毒理学
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发育生物学
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产品关联
DOI
PubMed
高影响因子
World Journal of Cardiology
·
2025
·Vol. 17
·期 12
·112062
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IF 2.8
Yu-Bao Zhao,Zhi-Hong Bao,Ya Tu,Xia Qiu,Yin-Lan Bao,Min Su,Hai-Jun Qi,Quan Wan
BACKGROUND Myocardial infarction (MI) occupies a very high mortality and morbidity rate, and the search for effective pharmacological treatments has far-reaching implications for clinical research. AIM To explore the protective effects of Mongolian medicine Agari-5 on rats with MI. METHODS Sprague-Dawley rats were used, and both the Agari-5 and model groups had their coronary arteries clamped to induce MI. Proteomics was used to research the potential mechanism of action while ELISA, hematoxylin and eosin, and Masson’s staining were used to preliminarily investigate the protective impact of Agari-5 on rats with MI. RESULTS The current study has shown that Agari-5 might enhance cardiac function indicators, including echocardiography results of rats and creatine kinase, creatine kinase isoenzyme, and lactate dehydrogenase, in rats that had MI. According to the results of pathological staining, Agari-5 may lessen inflammatory cell infiltration and cardiomyocyte fibrosis, among other things. The proteome analysis revealed that there were 60 distinct proteins in total, four of which were associated with the heart. The expression of PSAT1, PDK1, SMAD4, and SDF2 proteins may be linked to the mechanism of their protective effects. CONCLUSION Potential therapeutic effects of Agari-5 for MI and its mechanism of action may be related to PSAT1, PDK1, SMAD4, and SDF2.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Yuhang Gong,Da Wo,Canran Wang,Ruokun Huang,En Ma,Celiang Wu,Jun Peng,Weidong Zhu,Dan-ni Ren
Background: Angiotensin II (Ang II) type 1 receptor (AT1R) signaling pathway is a key component of the renin-angiotensin-aldosterone system (RAAS) that is involved in the development of hypertension. Chronic Ang II overactivation results in pathological cardiac hypertrophy that progresses into decompensated cardiac dysfunction and impairment. Qingda granule (QDG) is a Traditional Chinese formula that has been used clinically in treating hypertension and its complications.Purpose: This study aimed to elucidate the role and underlying mechanisms of QDG in preventing Ang II-induced cardiac hypertrophy.Methods: We used chronic Ang II infusion via minipumps in mice and administered QDG daily to examine the effects of QDG on preventing hypertension and various parameters of cardiac impairment.Results: QDG treatment significantly reduced Ang II-induced elevation in blood pressure. Furthermore, QDG exerted a robust cardioprotective effect on chronic Ang II-induced cardiac hypertrophy and decompensated cardiac dysfunction. QDG also inhibited Ang II-induced adverse NF-κB signaling activation and downstream pro-inflammatory targets, which were prevented via administration with SC75741, a specific NF-κB inhibitor.Conclusion: Our findings provide further insight into the robust ability of QDG in preventing Ang II-induced cardiac hypertrophy via preventing NF-κB signaling activation and implicate its use in the clinical treatment of hypertension and cardiac hypertrophy.
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DOI
PubMed
高影响因子
WASTE MANAGEMENT
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2023
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IF 8.1
Lin Zhu,Caihong Huang,Wei Li,Weixia Wu,Zhurui Tang,Yu Tian,Beidou Xi
Nitrogen loss from compost is a serious concern, causing severe environmental pollution. The NH 4 + -N content reflects the release of NH 3 . However, the nitrogen conversion pathway that has the greatest impact on NH 4 + -N content is still unclear. This study attempted to explore the key pathways, core functional microorganisms, and mechanisms involved in the transformation of ammonia nitrogen during composting. KEGG (Kyoto Encyclopedia of Genes and Genomes) metabolic pathways revealed that ammonia assimilation was dominated by the glutamate dehydrogenase (GDH) pathway (53.4%), which is crucial for nitrogen preservation. The combined analysis of KEGG, NR species annotation, and co-occurrence network identified 20 easy-to-regulate obligate core nitrogen-transforming functional microorganisms, including 18 ammonia-assimilating bacteria. Furthermore, the effects of environmental parameters on the obligate core functional microorganisms were investigated. The present study results provided a theoretical basis for the utilization of ten ammonia-assimilating bacteria, such as Paenibacillus , Erysipelatoclostridium, and Defluviimonas to improve the quality of compost.
研究领域:
环境科学
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微生物学
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堆肥技术
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2020
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IF 7.539
Jiashun Cao,Boming Fu,Teng Zhang,Yang Wu,Ziyan Zhou,Jianan Zhao,E Yang,Tangjian Qian,Jingyang Luo
In this study, the distribution, removal efficiency, and potential risks of 9 typical endocrine active compounds (EACs) in two full-scale wastewater treatment plants (WWTPs) were investigated. The EAC concentrations ranged from 0.2 to 7394.2 ng/L in influents. The source of influents was a critical factor in determining the EAC levels. EACs were primarily removed in the secondary biological processing units, with removal efficiencies fluctuating from 13.7% to 98%. The biological treatment processes and operating parameters ( i.e., HRT and SRT) influenced the EAC removal efficiency. Bisphenol A (BPA) and estriol were mainly removed by biodegradation, while antidepressants were primarily eliminated by sludge adsorption according to the distribution patterns and mass flow of EACs in WWTPs . Novosphingobium, Saprospiraceae, etc. were the core functional bacteria for EAC biodegradation. In addition, sertraline in effluents and dewatered sludge may pose medium environmental risks, while the other EACs pose low environmental risks.
研究领域:
环境科学
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水处理
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毒理学
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微生物学
✓ 已验证
DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2024
·
IF 12.7
Shifen Li,Wajiha Ahmed,Shuqin Wang,Xinman Hu,Beiduo Wang,Zhaoyi Wang,Liyin Shen,Yang Zhu,Changyou Gao
The adhesion between heart and chest post-operation raises a significant challenge for the application of cardiac patches due to its severe influences on the recovery of heart function and increased risk of re-treatment. Anabatic inflammatory response and increased oxidative stress aggravate the damage to heart functions. Herein, a multi-functional cardiac patch was manufactured by successive electrospinning of two types of novel selenium-containing polyurethanes (PU), followed by click grafting of poly (ethylene glycol) (PEG) molecules on the top layer. This patch possessed dual-functions of postoperative anti-adhesion and anti-oxidative stress for efficient therapy post myocardial infarction (MI). The high redox reactivity of diselenium bonds were introduced into the main chains of PU, which reduced the level of reactive oxygen species (ROS) and modulated the inflammatory environment, alleviating the synechia formation simultaneously. The cardiac patch could effectively resist fibroblast adhesion, regulate the expression of vinculin and tissue-type plasminogen activator (t-PA), and inhibit the synechia formation. Meanwhile, it also restored significantly the heart function post MI by decreasing the local inflammation, facilitating less collagen formation, and improving cell signal transduction and angiogenesis. Collectively, this novel patch achieved anti-adhesion and therapeutic effect synergistically, bringing advancement in cardiac patch design and potential for translation of medicinal devices.
研究领域:
生物材料
·
心血管工程
·
组织工程
·
再生医学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）