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Genes · 2020 ·Vol. 11 ·期 5 ·478 · IF 3.759

Molecular Mechanism Underlying Mechanical Wounding-Induced Flavonoid Accumulation in Dalbergia odorifera T. Chen, an Endangered Tree That Produces Chinese Rosewood

Ying Sun,Mei Gao,Seogchan Kang,Chengmin Yang,Hui Meng,Yun Yang,Xiangsheng Zhao,Zhihui Gao,Yanhong Xu,Yue Jin,Xiaohong Zhao,Zheng Zhang,Jianping Han

Dalbergia odorifera, a critically endangered tree species, produces heartwood containing a vast variety of flavonoids. This heartwood, also known as Chinese rosewood, has high economic and medicinal value, but its formation takes several decades. In this study, we showed that discolored wood induced by pruning displays similar color, structure, and flavonoids content to those of natural heartwood, suggesting that wounding is an efficient method for inducing flavonoid production inD. odorifera. Transcriptome analysis was performed to investigate the mechanism underlying wounding-induced flavonoids production inD. odoriferaheartwood. Wounding upregulated the expression of 90 unigenes, which covered 19 gene families of the phenylpropanoid and flavonoid pathways, including PAL, C4H, 4CL, CHS, CHI, 6DCS, F3’5’H, F3H, FMO, GT, PMAT, CHOMT, IFS, HI4’OMT, HID, IOMT, I2’H, IFR, and I3’H. Furthermore, 47 upregulated unigenes were mapped to the biosynthesis pathways for five signal molecules (ET, JA, ABA, ROS, and SA). Exogenous application of these signal molecules resulted in the accumulation of flavonoids in cell suspensions ofD. odorifera, supporting their role in wounding-induced flavonoid production. Insights from this study will help develop new methods for rapidly inducing the formation of heartwood with enhanced medicinal value.Keywords:Dalbergia odorifera;flavonoid biosynthesis;heartwood;mechanical wounding;signals;transcriptome

研究领域: 植物生理学 · 分子生物学 · 药理学
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Materials Research Express · 2020 ·Vol. 7 ·期 11 ·115302 · IF 1.929

Preparation, characterization and foaming performance of thermally expandable microspheres

Furong Li,Qingqing Zhang,Shouzheng Jiao,Zhicheng Sun,Jinyue Wen,Xiaoyang Du,Ruping Liu,Luhai Li

Thermal expansion microcapsules (TEMs) are widely used in various fields due to their unique structures. In recent years, TEMs have attracted much attention and have broad market application prospects. In this study, thermally expandable microcapsules with a core–shell structure were prepared by suspension polymerization using acrylonitrile (AN), methyl methacrylate (MMA), and methyl acrylate (MA) as monomers and low-boiling alkane as the core material. Through particle size analysis, morphology test, thermal analysis and other methods, the effects of core material types, single core material and mixed core material, dispersion system on the microcapsule structure, particle size distribution, and expansion properties were compared. Moreover, the core material with a content of 35% can make the expansion ratio of the microcapsules up to 4 times. The expansion performance of the microcapsules with a mixture of isopentane and isooctane (ratio 1:1) as the core material was increased by 27% compared with that of a single core material. In addition, comparing with colloidal SO2/PVP dispersant, the expansion ratio of the microcapsules with magnesium hydroxide as the dispersant was increased by 20%. Finally, the optimized method for preparing thermally expandable microcapsules was obtained.

研究领域: 材料科学 · 化学工程
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CHEMICAL ENGINEERING JOURNAL · 2025 · IF 13.2

Microwave-assisted deep eutectic solvent extraction of lignin from spruce heartwood and sapwood, targeting the comparison of different biorefinery concepts

Jiayun Xu,Chuanling Si,Lin Dai,Jarl Hemming,Andrey Pranovich,Chunlin Xu

Lignin-first fractionation (microwave-assisted deep eutectic solvent fractionation, MW-DES) and carbohydrate-first fractionation (hot water extraction followed by MW-DES, HWE-MW-DES), were compared while pretreating spruce heartwood (HW) and sapwood (SW) for lignin extraction. The lignin recovery yield was 80 % for HW and 74 % for SW, while SW demonstrated high selectivity in delignification, achieving 86 % removal of lignin with only minor cellulose loss (3.6 %) treated with ChCl/LA (1:2) at 145 °C for 10 min. The carbohydrate-first approach gives an opportunity to obtain lignin with a lower carbohydrate content (∼0.5 %) and higher lignin purity (88 %). The structure of lignin exhibited no significant differences after two different fractionation approaches, with the phenolic hydroxyl content in lignin in the amount of 2.59/2.73 mmol/g (MW-DES) and 2.54/2.74 mmol/g (HWE-MW-DES) from HW/SW. The above provides a theoretical basis for developing and optimizing the valorization of lignin from spruce HW/SW, employing either lignin-first or carbohydrate-first fractionation approaches of the sustainable biorefinery processes.

CAS / MDL: 67-48-1 50-21-5
研究领域: 林产化学与工业 · 生物质能工程
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