REACTIVE & FUNCTIONAL POLYMERS
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2022
·
IF 4.966
Xiao Li Li,Peng Liu,Bin Chu,Jin Mei He,Zhen Wang,Song Wang,Chang Sheng Chen,Xiao Hong Ge,Hui Li,Wei Qiang Liu
Myocardial infarction (MI) is a common heart emergency that potentially causes death. Injectable hydrogels with excellent malleability and filling properties are suitable for myocardial injection and can avoid the need for open surgery. In this study, dopamine grafted hyaluronic acid (HADOPA) was synthesized and combined with sodium alginate (Alg) and Ca 2+ -loaded liposomes (Ca-FVI) to prepare a series of injectable thermosensitive sodium hyaluronate/sodium alginate hydrogels (HADOPA/Ca-FVI/Alg). At 37 °C, the HADOPA/Ca-FVI/Alg solution rapidly changed from liquid to hydrogel because of the crosslinking between sodium alginate and the Ca 2+ released from Ca-FVI at a phase transition time of 100–200 s. The HADOPA/Ca-FVI/Alg scaffolds exhibited a porous 3D network structure, controlled porosity, and connected pore properties. Increasing the proportion of HADOPA increases the elasticity and absorbance of hydrogels. Experimental assessment showed that the hydrogels have good biocompatibility and that Alg1-HADOPA is more conducive to the adhesion, proliferation, and spreading of mouse cardiac myocytes while maintaining the best cell state. These findings suggest that HADOPA/Ca-FVI/Alg hydrogels improve cell proliferation and tissue repair, and that they are a promising therapeutic option for cardiac repair after MI.
研究领域:
生物材料学
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心血管医学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Xiaotong Zhao,Jingyi Li,Jing Dai,Zhan Qu,Xiaoyun Li,Yong Wu,Shijun Hu,Jian Fang,Zhenya Shen,Miao Xiao,Mingliang Tang
Bioelectricity plays a pivotal role in human physiology and pathology, particularly within the cardiovascular system. Myocardial ischemia disrupts the normal transmission of electrical signals through the heart, leading to cardiomyocyte death, ventricular remodeling, and ultimately heart failure. Electrical stimulation (ES) has been proven to be an effective strategy for treating cardiac diseases. However, invasive electrode implantation and the unique anatomical position of the heart limit the clinical application of ES therapy. Developing wireless ES systems that effectively enhance bioelectric propagation and electrical activity in the heart can significantly advance therapeutic interventions. In this study, a lead-free, biodegradable and the Food and Drug Administration approved poly-L-lactic acid piezoelectric nanofiber is designed for wireless ES to the myocardium under ultrasonic stimulation, thus promoting structural and functional recovery of cardiac tissue in murine myocardial infarction model. Further study shows that the ultrasound-driven wireless ES system significantly enhanced mitochondria function and angiogenesis in vivo and in vitro experiments. Additionally, the ES system can regulate intracellular calcium ion concentration and cardia tissue contraction rhythm of isolated neonatal rat. Collectively, the findings offer a novel approach for the treatment of heart diseases and potential cardiac pacing applications under the ultrasound-driven wireless ES system.
研究领域:
生物医学工程
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心血管疾病治疗
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材料科学
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产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Xianghua Zhong,Li Luo,Jiyuan Wu,Weirun Li,Xinyang Liu,Tenghui Ye,Zhenhua Li,Peng Shi
Mesenchymal stromal cell (MSC) therapy holds great promise for treating myocardial infarction (MI). However, the inflammatory and reactive oxygen species (ROS)-rich environment in infarcted myocardium challenges MSC survival, limiting its therapeutic impact. In this study, we demonstrate that chemical modification of MSCs with anti-VCAM1 and polydopamine (PD) significantly enhances MSC survival and promotes cardiac repair. Anti-VCAM1 modification facilitates MSC adhesion to inflamed tissue, ensuring MSC retention in the injured myocardium, while PD scavenges ROS surrounding MSCs, creating a conducive environment for cell transplantation. Our data indicate that chemically engineered MSCs effectively disrupt the inflammation-ROS cycle and modulate inflammation-related immune responses, thus improving MI microenvironments. Single-cell RNA sequencing of rat hearts reveals that treatment with engineered MSCs inhibits cardiac fibrosis by suppressing HB-EGF–EGFR signaling between anti-inflammatory macrophages and activated fibrillates. Ultimately, engineered MSCs demonstrate superior therapeutic efficacy in a rat model of MI. This study presents a straightforward, safe, and efficient chemical method for enhancing MSC therapy.
研究领域:
再生医学
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心血管病学
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细胞治疗
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产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Yao Chen,Zhanghao Huang,Cheng Ji,Jia-Hai Shi
Background Biomaterials can improve cardiac repair combined with transplantation of bone marrow mononuclear cells (BMMNCs). In this study, we compared the phenotype and cardiac repair between human heart valve-derived scaffold (hHVS) and natural protein/polycaprolactone (NP/PCL) anchored BMNNCs. Methods and results BMMNCs were obtained from mice five days following myocardial infarction. Subsequently, BMMNCs were separately cultured on hHVS and PCL. Proliferation and cardiomyogenic differentiation were detected in vitro. Cardiac function was measured after transplantation of cell-seeded cardiac patch on MI mice. After that, the BMMNCs were collected for mRNA sequencing after culturing on the scaffolds. Upon anchoring onto hHVS or PCL, BMMNCs exhibited an increased capacity for proliferation in vitro, however, the cells on hHVS exhibited superior cardiomyogenic differentiation ability. Moreover, both BMMNCs-seeded biomaterials effectively improved cardiac function after 4 weeks of transplantation, with reduced infarction area and restricted LV remodeling. Cell-seeded hHVS was superior to cell-seeded PCL. Conclusion BMMNCs on hHVS showed better capacity in both cell cardiac repairing and improvement for cardiac function than on PCL. Compared with seeded onto PCL, BMMNCs on hHVS had 253 genes up regulated and 189 genes down regulated. The reason for hHVS’ better performance than PCL as a scaffold for BMMNCs might be due to the fact that optimized method of decellularization let more cytokines in ECM retained.
研究领域:
心血管医学
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再生医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Shu-yi He,Lin-yu Long,Zhi-cun Wang,Wen-qi Liu,Wen Zhang,Cheng Hu,Xiao-feng Wu,Ruiqi Dong,Hong-song Fan,Qing-Jiang,Ying-ying Tong,Guang-hua Yang,Li-Yang,Yun-bing Wang
The postinfarction myocardium is in an environment of ischemia and hypoxia, and the damaged myocardium develops into scar tissue lacking electrical conductivity after a series of pathological changes. Thus, the regeneration of blood perfusion and electrical connections is crucial to repair the injured myocardium. In this study, an injectable self-doping conductive hydrogel composed of functionalized carboxymethyl chitosan (CMC) and oxidized hyaluronic acid (OHA) is developed for human umbilical cord mesenchymal stem cell (HUMSC)-derived secretome delivery to protect cardiac function after myocardial infarction (MI). In vitro experiments shows that the conductive hydrogel had good biocompatibility and conductivity consistent with the natural myocardium, and the secretome presents a good ability to promote cell proliferation, migration and angiogenesis . In a rat MI model, echocardiography shows an improvement in ejection fraction and fraction shortening after hydrogel injection. Additionally, susceptibility to arrhythmia is decreased in the standard clinically programmed electrical stimulation (PES) protocol. Immunofluorescence staining demonstrates that hydrogel treatment reduces cardiomyocyte apoptosis and oxidative stress, improves cardiomyocyte morphology and function, promotes angiogenesis. Overall, this study demonstrated the application potential of a conductive hydrogel combined with a cell-free strategy to reconstruct electromechanical coupling in infarcted myocardium and promote cardiac repair and regeneration.
研究领域:
心脏病学
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组织工程
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再生医学
✓ 已验证
DOI
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
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2023
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IF 5.4
Ping Ping,Shasha Guan,Chaoxue Ning,Ting Yang,Yali Zhao,Pei Zhang,Zhitao Gao,Shihui Fu
Acute myocardial infarction (AMI) is a common cardiovascular condition that progressively results in heart failure. In the present study, we have designed to load transforming growth factor beta 3 (TGF-β3) and cardio potential exosomes into the blended polycaprolactone/type I collagen (PCL/COL-1) nanofibrous patch (Exo@TGF-β3@NFs) and examined its feasibility for cardiac repair . The bioactivity of the developed NFs towards the migration and proliferation of human umbilical vein endothelial cells was determined using in vitro cell compatibility assays. Additionally, Exo@TGF-β3/NFs showed up-regulation of genes involved in angiogenesis and mesenchymal differentiations in vitro . The in vivo experiments performed 4 weeks after transplantation showed that the Exo@TGF-β3@NFs had a higher LV ejection fraction and fraction shortening functions. Subsequently, it has been determined that Exo@TGF-β3@NFs significantly reduced AMI size and fibrosis and increased scar thickness. The developed NFs approach will become a useful therapeutic approach for the treatment of AMI.
研究领域:
生物医学工程
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心血管病学
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2023
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IF 5
Shaoyi Lin,Yunyun Zhu,Tingting Hu,Kaihan Wang,Xiaomin Chen
Acute myocardial infarction (AMI) has caused cardiac vascular disease to emerge in recent years, and heart failure is the leading cause of death worldwide and modern medicine offers a number of treatments for AMI. In the present investigation, we created a new hydrogel material for the treatment of acute MI repair that combines mesenchymal stem cell (MSCs)-derived exosomes (EX) and selenium nanoparticles (Se NPs)-loaded collagen type 1 (COL-I)/tannic acid (TA) hydrogel. The physico-chemical characterizations of prepared Se NPs were systematically analysed by efficient analytical and spectroscopic methods. In vitro cytocompatibility analyses, established that prepared hydrogel samples have greater cell viability by MTT and LDH assays, which described that the suitability of hydrogel for the AMI treatment. In vitro cell migration assay exhibited that ∼95% of cell migration ability for Se-EX/Hydrogel when compared to the other groups on cardiac endothelial cells (cECs). The qRT-PCR analysis revealed that Se-EX/Hydrogel provided 3-fold decreasing of pro-inflammatory factors expressions when compared to the LPS induced cell models (cECs and Raw 264.7 cells). In vitro cell apoptosis observations demonstrated that developed Se-EX/Hydrogel effectively reduced the LPS-induced cell apoptosis (13.59 ± 1.2) when compared to the other groups (LPS = 29.51 ± 0.9; LPS + EX = 17.65 ± 1.0; LPS + Se NPs = 18.38 ± 0.8). When blended Se-EX/Hydrogel group were injected into AMI rat models, in vivo observation revealed that improved cardiac function through increased LV wall thickness (2.46 ± 0.6 mm) and significantly reduced infarct size (12.05 ± 1.0%) when compared to the AMI group. The outcome of the results demonstrated that introduced injectable hydrogel had a significant impact on the recovery of infarcted heart.
研究领域:
心血管疾病
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纳米医学
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干细胞治疗
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药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
Cell Stem Cell
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2025
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IF 20.4
Lei Fan,Qi Tang,Yutong Wang,Hang Sun,Ge Li,Yi Yang,Huiying Zhu,Zhaoyuan Liu,He Wang,Hongyan Wang,Qing Jing,Christopher L. Antos,Hui Xiao,Renchu Guan,Gang Pei,Florent Ginhoux,Zhigang Zhou,An Zeng
Adult hearts scar after injury, while neonatal hearts regenerate. The mechanisms underlying this dichotomy remain unclear. Through comparative spatiotemporal single-cell analyses and dual recombinase-mediated lineage tracing, we uncovered an injury-induced Clusterin + cardiomyocyte ( Clu + CM) population that coordinates reparative, anti-inflammatory macrophage activity. Following injury, Clu + CMs emerge in the border zone of regenerative hearts but are scarce in non-regenerative contexts. These CMs secrete CLU, which binds to macrophage Toll-like receptor 4 (TLR4), attenuating inflammation and promoting reparative polarization through Cpt1a -dependent fatty acid oxidation. These macrophages secrete bone morphogenetic protein 2 (BMP2), activating bone morphogenetic protein receptor, type 1A (BMPR1A) signaling in CMs to drive proliferation. Reduced CLU levels in myocardial infarction patients correlate with impaired cardiac function, whereas Clu overexpression or transplantation of engineered CLU + human cardiac organoids recapitulates this regenerative modulation, enhancing myocardial repair in adult mice. Our findings reveal a critical cardio-immune mechanism whereby Clu + CMs reprogram macrophages to resolve inflammation and stimulate CM proliferation, providing potential strategies for cardiac regeneration.
研究领域:
心脏病学
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免疫学
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再生医学
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分子生物学
·
细胞信号传导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
American Journal of Translational Research
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2016
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IF 3.146
Yang Liu,Yachen Xu,Zhenhua Wang,Dezhong Wen,Wentian Zhang,Sebastian Schmull,Haiyan Li,Yao Chen,Song Xue
Electrospun nanofibrous sheets get increasing attention in myocardial infarction (MI) treatment due to their good cytocompatibility to deliver transplanted stem cells to infarcted areas and due to mechanical characteristics to support damaged tissue. Cardiac extracellular matrix is essential for implanted cells since it provides the cardiac microenvironment. In this study, we hypothesized high concentrations of cardiac nature protein (NP), namely elastin and collagen, in hybrid polycaprolactone (PCL) electrospun nanofibrous sheets could be effective as cardiac-mimicking patch. Optimal ratio of elastin and collagen with PCL in electrospun sheets (80% NP/PCL) was selected based on cytocompatibility and mechanical characteristics. Bone-marrow (BM) c-kit + cells anchoring onto NP/PCL sheets exhibited increased proliferative capacity compared with those seeded on PCL in vitro. Moreover, we examined the improvement of cardiac function in MI mice by cell-seeded cardiac patch. Green Fluorescent Protein (GFP)-labeled BM c-kit + cells were loaded on 80% NP/PCL sheets which was transplanted into MI mice. Both 80% NP/PCL and c-kit + -seeded 80% NP/PCL effectively improved cardiac function after 4 weeks of transplantation, with reduced infarction area and restricted LV remodeling. C-kit + -seeded 80% NP/PCL was even superior to the 80% NP/PCL alone and both superior to PCL. GFP + cells were identified both in the sheets and local infarcted area where transplanted cells underwent cardiac differentiation after 4 weeks. To the best of our knowledge, this is the first report that sheets with high concentrations of nature proteins loaded with BM c-kit + cells might be a novel promising candidate for tissue-engineered cardiac patch to improve cardiac repair after MI.
研究领域:
生物医学工程
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心脏病学
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Danrong Hu,Ran Li,Yicong Li,Meng Wang,Lu Wang,Shiqi Wang,Hongxin Cheng,Qing Zhang,Chenying Fu,Zhiyong Qian,Quan Wei
Myocardial infarction (MI) is a critical global health challenge, with current treatments limited by the complex MI microenvironment, particularly the excessive oxidative stress and intense inflammatory responses that exacerbate cardiac dysfunction and MI progression. Herein, a mannan-based nanomedicine, Que@MOF/Man, is developed to target the inflammatory infarcted heart and deliver the antioxidative and anti-inflammatory agent quercetin (Que), thereby facilitating a beneficial myocardial microenvironment for cardiac repair. The presence of mannan on the nanoparticle surface enables selective internalization by macrophages rather than cardiomyocytes. Que@MOF/Man effectively neutralizes reactive oxygen species in macrophages to reduce oxidative stress and promote their differentiation into a reparative phenotype, reconciling the inflammatory response and enhancing cardiomyocyte survival through intercellular communication. Owing to the recruitment of macrophages into inflamed myocardium post-MI, in vivo, administration of Que@MOF/Man in MI rats revealed the specific distribution into the injured myocardium compared to free Que. Furthermore, Que@MOF/Man exhibited favorable results in resolving inflammation and protecting cardiomyocytes, thereby preventing further myocardial remodeling and improving cardiac function in MI rats. These findings collectively validate the rational design of an inflammation-targeted delivery strategy to mitigate oxidative stress and modulate the inflammation response in the injured heart, presenting a therapeutic avenue for MI treatment.
研究领域:
药理学
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纳米技术
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心血管医学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-20
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IF 14.7
Qiu Renjie,Zhang Xingying,Song Chen,Xu Kaige,Nong Huijia,Li Yi,Xing Xianglong,Mequanint Kibret,Liu Qian,Yuan Quan,Sun Xiaomin,Xing Malcolm,Wang Leyu
Conductive cardiac patches can rebuild the electroactive microenvironment for the infarcted myocardium but their repair effects benefit by carried seed cells or drugs. The key to success is the effective integration of electrical stimulation with the microenvironment created by conductive cardiac patches. Besides, due to the concerns in a high re-admission ratio of heart patients, a remote medicine device will underpin the successful repair. Herein, we report a miniature self-powered biomimetic trinity triboelectric nanogenerator with a unique double-spacer structure that unifies energy harvesting, therapeutics, and diagnosis in one cardiac patch. Trinity triboelectric nanogenerator conductive cardiac patches improve the electroactivity of the infarcted heart and can also wirelessly monitor electrocardiosignal to a mobile device for diagnosis. RNA sequencing analysis from rat hearts reveals that this trinity cardiac patches mainly regulates cardiac muscle contraction-, energy metabolism-, and vascular regulation-related mRNA expressions in vivo. The research is spawning a device that truly integrates an electrical stimulation of a functional heart patch and self-powered e-care remote diagnostic sensor.
研究领域:
生物医学工程
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心脏病学
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纳米技术
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远程医疗
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Qingqing Wang,Shuangyuan Cao,Teng Zhang,Fanzhen Lv,Mingfei Zhai,Danmeng Bai,Mengzhen Zhao,Haoxin Cheng,Xiaolei Wang
Acute myocardial infarction (AMI) resulting from coronary artery occlusion stands as the predominant cause of cardiovascular disability and mortality worldwide. An all-encompassing treatment strategy targeting pathological processes of oxidative stress, inflammation, proliferation and fibrotic remodeling post-AMI is anticipated to enhance therapeutic outcomes. Herein, an up-down-structured bilayer microneedle (Ce-CLMs-BMN) with reactive oxygen species (ROS) and ultrasound (US) dual-responsiveness is proposed for AMI in-situ sequential therapy. The upper-layer microneedle is formulated by crosslinking ROS-sensitive linker with polyvinyl alcohol loaded with cerium dioxide nanoparticles (CeNPs) featuring versatile enzyme-mimetic activities. During AMI acute phase, prompted by ischemia-induced microenvironmental redox imbalance, this layer swiftly releases CeNPs, which aid in eliminating excessive ROS and catalyzing oxygen gas (O 2 ) production through multiple enzymatic pathways, thereby alleviating oxidative stress-induced damage and modulating inflammation. In AMI chronic repair phase, micro-nano reactors (CLMs) situated in the lower-layer microneedle undergo cascade reactions with the assistance of US irradiation to generate nitric oxide (NO). As a bioactive molecule with pro-angiogenic and anti-fibrotic effects, NO expedites cardiac repair while attenuating adverse remodeling. Additionally, its antiplatelet-aggregating properties contribute to thromboprophylaxis. In-vitro and in-vivo results substantiate the efficacy of this integrated healing approach in AMI management, showcasing promising prospects for advancing infarcted heart repair.
研究领域:
心血管疾病
·
纳米技术
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药物递送系统
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生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）