Introduction: The search for natural bioactive compounds that act as α-glucosidase inhibitors is a central focus in diabetes treatment research.Methods: This study utilized ultrasonic-assisted extraction to optimize the extraction of polyphenols from honeysuckle leaves through response surface methodology (RSM). Enzyme inhibition and fluorescence quenching experiments were conducted to examine the inhibitory activity and binding behavior of the extracted polyphenols.Results: The results indicated that the optimal conditions for polyphenol extraction were as follows: ethanol concentration, 64%, liquid–solid ratio, 45 mL/g, ultrasonic power, 700 W, ultrasonic time, 6 min. Under these conditions, the yield of polyphenols from honeysuckle leaves was 9.16 ± 0.19%, which closely aligns with the predicted value of 9.18%. The total phenolic content of the polyphenol extracts was 20.6 ± 0.67%, with chlorogenic acid and luteoloside contents measuring 5.65 ± 0.40% and 2.51 ± 0.14%, respectively. The inhibitory effect of polyphenol extracts (IC50, 0.14 ± 0.01 mg/mL) on α-glucosidase was better than that of chlorogenic acid (IC50, 0.55 ± 0.02 mg/mL). Fluorescence quenching experiments indicated that the polyphenol extracts interact with α-glucosidase, resulting in alterations to the microenvironment of amino acid residues.Discussion: This interaction can reduce the binding affinity between the substrate and α-glucosidase, thereby contributing to the objective of lowering postprandial hyperglycemia. Our research results can provide reference for the development and utilization of honeysuckle leaves.
Rong Zhenji,Yu Chunping,Wang Xin,Wang Ruyue,Gao Yang,Zhang Hailong
Blue honeyberry is a well-known nutritious fruit. Meanwhile, it is also an Oroqen herb in China. However, the chemical constituent of the leaves of the plant is little known, despite having better anti-inflammatory activity. In this study, we investigated the chemical constituents of blue honeyberry leaves and screened their anti-inflammatory effects in RAW264.7 cells. Thirty-nine compounds including a new fernane-type triterpenoid were isolated and identified using various chromatographic approaches and spectroscopic techniques. The new compound and some other components showed a significant anti-inflammatory effect by inhibiting nitric oxide production in RAW264.7 cells, in which compounds 6 and 38 exhibited the inhibition of 40.6 ± 4.8% and 46.0 ± 1.4% at the concentration of 100 μM, respectively, without inducing any cytotoxicity.
Nowadays, the recovery of added-value compounds by pre-treatment and extraction has become imperative in the recycling and transformation of bio-waste. Hence, to achieve efficient bio-waste recycling and avoid the drawbacks of solvent and time consumption in traditional pre-treatment, a green and facile method of efficient separation and enrichment of luteolin (LTL) from honeysuckle leaves has been developed via the green synthesis of novel magnetic molecularly imprinted polymers (Fe3O4-LTL-MIPs). Fe3O4-LTL-MIPs were prepared in aqueous solution using polyethyleneimine and dopamine as eco-friendly bifunctional monomers via layer-by-layer assembly onto the amino-modified Fe3O4 carriers. The thin sandwich-biscuit-like imprinting layer in Fe3O4-LTL-MIPs provides sufficient binding sites with enhanced uniformity and accessibility for the template molecules, thus facilitating excellent recognition ability and rapid mass transfer. The obtained nanomaterials show homogeneous morphology, good crystallinity, satisfactory superparamagnetism, ultra-fast adsorption kinetics, high recognition ability, and excellent reusability. Moreover, Fe3O4-LTL-MIPs as solid-phase extraction adsorbents exhibit high efficiency for the separation and enrichment of LTL from the honeysuckle leaves combined with HPLC analyses. Therefore, the Fe3O4-LTL-MIPs could be potentially exploited as a feasible and efficient way to meet the goals of green chemistry for selective recycling and enriching target compounds from complicated bio-waste matrices.
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