INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2024
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IF 5
Xueying Song,Jiamu Kang,Xingyan Wei,Liu Liu,Yongfeng Liu,Feng Wang
Shigella flexneri has the ability to contaminate pork and cause foodborne diseases. This study aimed to examine the effectiveness of linalool (a natural preservative) against S. flexneri and explore its potential application in contaminated pork. The results showed that linalool was capable of damaging the cell membrane and binding to the DNA of S. flexneri , and inhibiting biofilm formation and disrupting mature biofilms. The antibacterial effectiveness of linalool on the surface of pork was further demonstrated by analyzing the physicochemical properties of the pork ( i.e. , weight loss rate, pH value, color index, and TVB-N value) and its protein profiles. Linalool did not completely kill S. flexneri in pork at minimum bactericidal concentration (MBC) concentration and its antibacterial effect of linalool was stronger during the initial stage of storage. During storage, linalool influenced the abundance of specific proteins in the pork, particularly those involved in pathways related to fat metabolism. These findings offer novel insights into the antibacterial efficacy of linalool and its underlying mechanism in pork.
研究领域:
食品科学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 11
·4709-4718
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IF 4.813
Fan Qiuxia,Yuan Yahong,Jia Hang,Zeng Xuejun,Wang Zhouli,Hu Zhongqiu,Gao Zhenpeng,Yue Tianli
Abstract Shigella flexneri ( Sh. flexneri ), a common foodborne pathogen, has become one of the main threats to food safety and human health due to its high pathogenicity and persistent infection. The objective of this study was to explore the antimicrobial and anti-biofilm activities and the possible mechanism of thymoquinone (TQ) against Sh. flexneri . The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of TQ against Sh. flexneri were 0.4 and 0.5 mg/mL, respectively. TQ showed bactericidal activity against Sh. flexneri in culture medium and milk system. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) observations demonstrated that TQ could induce abnormal cell morphology and destroy cell membrane. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis suggested that TQ could inhibit protein synthesis in Sh. flexneri . Also, at sub-inhibitory concentrations (SICs), TQ exhibited an inhibitory effect on Sh. flexneri biofilm formation, which was confirmed by crystal violet quantitative analysis and SEM observation. Real-time quantitative PCR (RT-qPCR) analyses revealed that TQ downregulated the expression of genes involved in Sh. flexneri biofilm formation. Thus, TQ has potential as a natural antimicrobial and anti-biofilm agent to address the contamination and infection caused by Sh. flexneri . Key points • Antimicrobial and anti-biofilm activity of TQ on Shigella flexneri were investigated.
• TQ inhibited biofilm formation by Shigella flexneri.
• TQ provided a new strategy for Shigella flexneri control.
研究领域:
微生物学
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食品安全
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分子生物学
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DOI
PubMed
高影响因子
FOOD CONTROL
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2024
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IF 5.6
Yifei He,Jinxiang Wei,Lili Zhang,Yu Xia,Zhouping Wang,Junsong Yang
In this study, a dual-mode SERS-fluorescent aptasensor based on a DNA walker was developed for Shigella flexneri ( S . flexneri ). Fe@SiO2@QDs were connected with H1 and the walking chain (hybrid strands of aptamer and cDNA) to fabricate fluorescent probes, and Ag R6G @SiO 2 were connected with H2 to fabricate SERS tags. Aptamer (Kd = 42.6 ± 3.16 nM) selective binding of S . flexneri , left the walking chain. The Zn 2+ -specific DNAzyme in the cDNA was exposed thus driving the DNA walker. The sequence on H1 containing BHQ3 was cleaved from fluorescent probes to restore the fluorescence. The remaining H1 sequence could bind to the H2 to generate Raman signals. The detection limit of the fluorescence signal was 11 CFU/mL, and the Raman signal was 3 CFU/mL. In addition, the aptasensor was successfully applied to the detection of actual samples with recovery ranges of 98.35–107.27% and 97.57–105.91% for the fluorescence and SERS methods, respectively.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
高影响因子
Cell Reports
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2025
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IF 6.9
Mingfang Wang,Jia Zeng,Binbin Cui,Guangliang Wu,Xiayu Chen,Yikai Fu,Bing Gu,Yinyue Deng
It was previously demonstrated that intestinal pathogens have evolved different mechanisms to enhance their infection and colonization in the intestines of hosts. However, it is unclear how Shigella effectively survives in the presence of bile salts. Here, we report that the biofilm formation and pathogenicity of S . sonnei are induced by primary bile salts through the two-component system EvgS/EvgA. The response regulator EvgA controls the transcription of the cyclic AMP synthase-encoding gene. Furthermore, the effector protein CRP of the cyclic AMP (cAMP) signal not only positively controls the transcription of ydeH , a gene encoding cyclic di-GMP (c-di-GMP) synthase, but also forms a complex with YdeH to improve its catalytic production of c-di-GMP. Additionally, interacting with YdeH enhances the ability of CRP to bind to the promoter of ydeH . Our work provides insights into how S . sonnei utilizes cascade amplification of c-di-GMP to promote fitness and pathogenicity in response to primary bile salts.
研究领域:
微生物学
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分子生物学
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感染与免疫
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信号转导
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病原生物学
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环境应答
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
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2023
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IF 6
Yifei He,Jinxiang Wei,Pengfei Ma,Imran Mahmood Khan,Lili Zhang,Niazi Sobia,Yu Xia,Zhouping Wang
Shigella Sonnei ( S. sonnei ) is a common foodborne pathogen causing intestinal diseases. In this study, a sandwich-structured colorimetric and surface-enhanced Raman scattering (SERS) dual methods adaptation sensor was prepared for the detection of S. sonnei . Firstly, two aptamers, SS4.1-2 ( K d = 43.5 ± 2.78 nM) and SP1-2.2 ( K d = 23.3 ± 3.01 nM) with good specificity and affinity were obtained by truncation strategy. Then, Fe 3 O 4 @SiO 2 @Ag nanoparticles were utilized to capture bacteria, while Cu-TCPP (Fe)/AuNPs nanosheets were used as signal probes due to their oxidase activity and SERS signals. The detection limits of the aptasensor were as low as 37 CFU/mL for the colorimetric signal and 7 CFU/mL for SERS signal. Furthermore, the aptasensor was successfully applied to detect S. sonnei in real samples with 94.95–112.92% and 98.40–103.95% recoveries for colorimetric and SERS methods, respectively. A more rapid and accurate method to detect S. sonnei contamination in food was improved.
研究领域:
食品科学
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生物传感
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微生物学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Zuxin Liang,Xuling Liu,Zhiran Qin,Jingshu Li,Jianhai Yu,Xiaoen He,Li Zhu,Hongying Fan,Yushan Hu,Jinxiu Yao,Chenguang Shen,Chengsong Wan,Bao Zhang,Wei Zhao
This study aimed to inactivate Shigella flexneri using curcumin-based photosensitization and determine the sensitivity of S. flexneri to the combination of epsilon (ε)-polylysine (ε-PL) and curcumin-based photosensitization for decontamination. Bacterial numbers were reduced by 4.65 log CFU/mL after incubation with 80 μM curcumin and illumination for 20 min. Photosensitization resulted in cell morphology changes and increased cell permeability and protein degradation. Oxidative stress gene ( oxyR ) and efflux pump genes ( marA , acrA ) were upregulated. Decontamination of S. flexneri after incubation with curcumin and ε-PL for 1 h followed by photoactivation for 60 s has been investigated. It was found that the growth of bacteria was completely inhibited. Additionally, the number of bacteria was reduced by > 6 log CFU/mL. Experiments on the uptake of curcumin molecules by bacteria showed that ε-PL promoted the penetration of bacterial cells by curcumin, thus enhancing the effects of photosensitization.
研究领域:
微生物学
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食品科学
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生物技术
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
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IF 2.8
Yan-Long Zhang,Yu-Jun Shi,Bao-Jian Duan,Xian-Hua Wang
For separation of deoxyribonucleic acid (DNA), positively charged amino-modified magnetic nanoparticles (MN) can effectively adsorb negatively charged DNA through electrostatic interaction. However, the reported preparation of amino-modified MN is usually tedious and time-consuming. Therefore, a simple synthesis method of amino-modified MN is necessary for DNA extraction. Herein, a novel polyethyleneimine-coated MN (PMN) was fabricated by one-pot hydrothermal synthesis for high-efficient DNA extraction. The fabricated PMN showed numerous exposed amino groups, which not only could effectively capture DNA through electrostatic interaction, but also limited the aggregation of PMN during application. Under optimized adsorption conditions, the maximum adsorption capacity of PMN for DNA could reach 192.4 μg m g−1. Shigella flexneri ( S . flexneri ) has the highest mortality rate among Shigella species and has been selected as target model pathogenic bacteria. Based on the optimized extraction conditions, PMN-based magnetic solid-phase microextraction (MSPE) and quantitative real-time PCR (qPCR) were integrated for detection of S . flexneri . The limit of detection of the proposed strategy was 2.4 × 10 2 CFU mL −1 and obviously lower than the commercial kit. To prove the practicability, the PMN-based MSPE combined qPCR strategy was successfully used in the determination of S . flexneri in spiked real sample, and the recovery values were in the range of 95.1 % to 102.1 % for apple juice, 60.4 % to 85.7 % for pickled vegetable, 97.8 % to 99.5 % for pig liver and 95.1 % to 102.1 % for pig colon, respectively. Therefore, we believe that the resultant PMN have great potential to become a universal magnetic adsorbent for high-efficient DNA extraction from complex biological samples.
研究领域:
生物化学
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分子生物学
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医学检测
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
·
IF 4.8
Shihao Nan,Xinmiao Ren,Mengshi Xiao,Lin Zhu,Haijin Mou,Ningyang Li,Rong Li,Shaoping Nie,Xiaodan Fu
The deoxyhexose sugar l -fucose and fucosylated oligosaccharides (FCO) are essential for various biological processes in human body and associated gut microbiota. Bacterial exopolysaccharides offer a promising new source for preparing functional oligosaccharides. Here, FCO were obtained through acidolysis of fucose-containing exopolysaccharides with a molecular weight of 5.9 × 10 3 kDa, derived from Clavibacter michiganensis M2. Structural analysis revealed that the main disaccharide components of FCO exhibited a molecular weight of 326 Da and a monosaccharide composition of fucose, galactose, and glucose in a 3:1:2 ratio. Nuclear magnetic resonance indicated that the disaccharide fragments consisted of two backbones of α- Galp -(1 → 2)-α- Fucp and β- Glcp -(1 → 4)-β- Fucp , respectively. In vitro fermentation of FCO with human fecal cultures resulted in an increased abundance of Bifidobacterium and Bacteroides , and a reduction in Escherichia-Shigella and Fusobacterium . This research outlines a novel structural fucosylated oligosaccharide and its preparation method, suggesting its potential application as a promising new prebiotic.
研究领域:
微生物学
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生物化学
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食品科学
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胃肠病学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Huan Li,Saiqing Xu,Ying Xie,Qun Zhang,Shenghua Ding,Rongrong Wang,Fuhua Fu,Xiaobei Zhan
In this study, the curdlan-polyphenol complexes were constructed by a pH-driven method. The interaction between curdlan and various hydrophobic polyphenols (curcumin, quercetin, and chlorogenic acid) was investigated. Curdlan could self-assemble into particles for loading polyphenols through hydrogen bonding and hydrophobic interactions. The three polyphenols were embedded in curdlan in an amorphous state. The curdlan-curcumin complex showed the lowest viscoelasticity but exhibited the highest curcumin loading ability (34.04 ± 1.73 mg/g). However, the curdlan-chlorogenic acid complex emerged the opposite trend, indicating that the loading capacity was associated with the hydrophobicity of polyphenols. The antioxidant activity of curdlan significantly increased after combining with polyphenols, which could be maintained during in vitro simulated gastrointestinal digestion. In particular, the curdlan-quercetin complex exhibited the highest antioxidant activity and short-chain fatty acid concentration, which could influence gut microbiota composition by promoting the proliferation of Prevotella and inhibiting the growth of Escherichia_Shigella . In conclusion, the curdlan-polyphenol complexes prepared by an alcohol-free pH-driven method could effectively enhance the gastrointestinal stability of polyphenols as well as increase the antioxidant and prebiotic activities of curdlan, which could be applied as a functional ingredient to improve gut health.
研究领域:
食品科学
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营养学
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生物技术
·
药学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Peng Xu,Shuai Chen,Qi Fu,Siyi Zhu,Zheng Wang,Jie Li
This research endeavors to investigate the potential preventive and protective effects of chlorogenic acid (CGA), a bioactive component found in various foods, in the context of dextran sodium sulfate (DSS)-induced colitis in mice. The study reveals that CGA administration, at dosages of 150 and 300 mg/kg, significantly retards the progression of colitis. This is substantiated by observable enhancements in colon length, reduction in the disease activity index, and mitigation of colon tissue damage. Meanwhile, CGA acts as an antioxidant by reducing the oxidative stress associated with colitis inflammation and increasing the activity of antioxidant enzymes. Furthermore, CGA demonstrates anti-inflammatory properties by inhibiting the inflammatory response associated with colitis and regulating the excessive expression of cytokines. Notably, CGA contributes to the restoration of intestinal barrier function by augmenting the levels of claudin-1 and ZO-1. Moreover, the investigation uncovers CGA's capacity to modulate the gut microbiota composition in colitic mice, fostering the proliferation of beneficial bacteria, notably Lactobacillus , while concurrently impeding the growth of detrimental bacteria like Bacteroides , Enterobacteriaceae, and Escherichia_Shigella . To conclude, these findings substantiate the viability of CGA for diverse applications in the realms of food and medicine, thereby bolstering its potential as a therapeutic strategy within dietary interventions.
研究领域:
药理学
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免疫学
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胃肠病学
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微生物学
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Zhentao Yao,Ge Zhang,Xin Wang,Jinjun Li,Yuting Qi,Yuanrong Jiang,Jie Gu,Liying Zhu,Xianrong Xu,Xiaoqiong Li
Processing techniques have been shown to alter the nutritional composition of rice bran (RB) and wheat bran (WB), influencing their ability to modify microbiota, but a systematic comparison is currently lacking. This study aimed to comprehensively investigate the effects of various processing methods, including physical modifications (grinding, extrusion, and steam explosion), enzymolysis (amylase, hemicellulose, and xylanase), and their combinations, on the modification of gut microbiota by RB and WB. Processing methods significantly altered the nutrient components of RB and WB as well as their capacity for gas and SCFAs production during in vitro fermentation. The RB23 (amylase treatment), RB31 (extrusion and hemicellulose treatment), and RB33 (steam explosion and xylanase treatment) samples exhibited the highest production of total SCFAs and butyric acid during in vitro fermentation. Overall, the microbiota responses to brans were conserved within individuals and the processing methods explained 21.6% of the variance. Fermentation of RB23, RB31, and RB33 samples was observed to enhance the richness of gut microbiota, decrease the Firmicutes/Bacteroidetes ratio, and stimulate the growth of Butyricicoccus and Faecallbacterium, while inhibiting the growth of Bilophila , Fusobacterium , and Escherichia-shigella . These findings may provide valuable insights for the processing of RB and WB.
研究领域:
食品科学
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微生物学
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营养学
·
生物技术
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jiamu Kang,Liu Liu,Yongfeng Liu,Xiaoyu Wang
Antibiotic resistance and capacity for biofilm formation of Shigella flexneri render previous prevention and control strategies minimally effective. Ferulic acid (FA) has been demonstrated to be useful due to its application in foods as an alternative natural preservative. However, information regarding the S. flexneri phenotype and molecular responses to FA exposure is limited. The present study investigated the effects of FA on S. flexneri planktonic growth and biofilm formation. The results demonstrated that the cell membrane of S. flexneri in planktonic growth mode exhibited irreversible destruction after FA exposure, as characterized by decreased cell viability, leakage of cytoplasmic constituents, accelerated adenosine triphosphate (ATP) consumption, cell membrane depolarization, and cellular morphological changes. FA significantly inhibited S. flexneri adhesion and biofilm formation at a working concentration (1/8 MIC) that almost did not inhibit planktonic growth. Transcriptomics profiling showed that the exposure to a subinhibitory concentration of FA dramatically altered gene expression in the S. flexneri biofilm, as a total of 169 differentially expressed genes (DEGs) were upregulated and 533 DEGs were downregulated, compared to the intact biofilm. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the DEGs were mainly involved in pathways of ribosomes, ABC transporters, and the citrate cycle. Furthermore, we show that FA altered the transcription of S. flexneri genes associated with adhesion, transcriptional regulation, and the synthesis and transport of extracellular polymeric substances that contribute to biofilm formation. These data provide novel insights into S. flexneri behavioral responses to FA exposure and suggest that FA could effectively constrain S. flexneri and its biofilm formation.
研究领域:
微生物学
·
分子生物学
·
食品科学
·
药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）