JOURNAL OF CHROMATOGRAPHY A
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2016
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IF 3.926
Hailong Peng,Mei Luo,Hua Xiong,Ningxiang Yu,Fangjian Ning,Jieping Fan,Zheling Zeng,Jinhua Li,Lingxin Chen
Photonic-magnetic responsive molecularly imprinted microspheres (PM-MIMs) were prepared by seed polymerization, through suitable functionalization of magnetic nanoparticles for further coating with photoresponsive functional monomer and imprinted layers, and then were successfully applied to the fast and selective extraction of 17β-estradiol (17β-E 2 ) from real samples. The PM-MIMs possessed a sandwich micro-spherical structure containing Fe 3 O 4 core, SiO 2 middle layer, and MIPs shell with thickness of 25 nm. The PM-MIMs displayed excellent photoresponsive properties and could be rapidly separated from solutions under an external magnet. The PM-MIMs had specific affinity towards 17β-E 2 with high adsorption capacity ( Q max = 0.84 mg g −1 ) and fast binding kinetics ( K d = 26.08 mg L −1 ). The PM-MIMs proved to be an ideal photoswitch with the ability of reversible uptake and release of 17β-E 2 upon alternate 365 and 440 nm irradiation: 45.0% of 17β-E 2 released from the PM-MIMs upon 365 nm irradiation, and 94.0% of the released 17β-E 2 was rebound to the PM-MIMs at 440 nm. Accordingly, the PM-MIMs were applied for fast separation and extraction of 17β-E 2 followed by HPLC-UV determination, presenting the low limit of detection (LOD, S/N = 3) and quantification (LOQ, S/N = 10) of 0.18 and 0.62 μmol L −1 , respectively. The high recoveries for spiked milk powder and drinking water samples were in the range of 97.5–113.0% with relative standard deviations less than 4.4%. This study reasonably combined photonic response, magnetic separation and surface imprinting, which endowed the PM-MIMs with significant advantages of high adsorption capacity and fast binding kinetics, convenient separation and recycled use, and simple rapid eco-benign adsorption/elution processes for template molecules. Thus, the PM-MIMs based method may be a simple, rapid, convenient, cost-effective and environmentally-friendly way for simultaneous separation, enrichment and detection of trace 17β-E 2 in complicated matrices.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2022
·Vol. 45
·期 14
·2687-2698
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IF 3.614
Hong Pan,Zushan Gan,Haoyun Hu,Cheng Liu,Yipeng Huang,Guihua Ruan
In this study, monodisperse magnetic carbon microspheres were successfully synthesized through the carbonization of phenolic resin encapsulated Fe 3 O 4 core-shell structures. The magnetic carbon microspheres showed high performance in ultrafast extraction and separation of trace triazine herbicides from environmental water samples. Under optimized conditions, both the adsorption and desorption processes could be achieved in 2 min, and the maximum adsorption capacity for simazine and prometryn were 387.6 and 448.5 μg/g. Coupled with high-performance liquid chromatography-ultraviolet detection technology, the detection limit of triazine herbicides was in the range of 0.30–0.41 ng/mL. The mean recoveries ranged from 81.44 to 91.03% with relative standard deviations lower than 7.47%. The excellent magnetic solid-phase extraction performance indicates that magnetic carbon microspheres are promising candidate adsorbents for the fast analysis of environmental contaminants.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2022
·
IF 4.601
Mengyuan Li,Pengfei Li,Yehong Han,Dandan Han,Hongyuan Yan
In this study, a new rapid and inexpensive filter solid-phase extraction method (FSPE) was developed for the extraction and separation of five perfluorinated carboxylic acids (PFCAs) (namely, perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluoroundecanoic acid, and perfluorododecanic acid) from milk samples. Commercial nylon 66 syringe filters were used as adsorbents without additional modification. The proposed method could achieve the fast adsorption of analytes by benefitting from the advantages of using the nylon 66 filter membrane on PFCAs with multiple adsorption interactions, namely, hydrophobic interactions and hydrogen bonds. The FSPE was designed to achieve the rapid isolation of analytes based on the rapid solid-liquid separation while using the solid-phase extraction disk. PFCAs residues in milk were extracted by first flowing the samples through a nylon 66 syringe filter from top to bottom. Double contact desorption with methanol was then accomplished by pulling and pushing the syringe, followed by derivatization with acetyl chloride-methanol. Finally, the extracted PFCAs residues were monitored using gas chromatography-tandem mass spectrometry (GC-MS/MS) quantification. Under optimal conditions, the established method presented good linearity (correlation coefficient ≥ 0.9996), precision (relative standard deviation ≤ 13%), accuracy (81–105%), sensitivity (limits of quantification: 4–18 ng kg −1 ) and extraction performance (10 min).
研究领域:
分析化学
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食品安全
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
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2023
·
IF 5
Jia Deng,Xiaojing Yuan,Lian Wang,Yanqi Sun,Hao He,Zhenyi Wu,Zibin Gao,Jingou Ji,Bochu Wang,Shilei Hao
Autogenous keratin is a promising material for personalized wound healing applications. However, the common keratin extraction methods are time-consuming, and the variations in performance between different individual keratins are not clear. Herein, we developed TiO2 photocatalytic-assisted H2O2 oxidation strategy (H2O2/TiO2/UV), an advanced oxidation method, to rapidly extract human hair keratins within 2 h. The H2O2/TiO2/UV method for keratin extraction showed a higher protein yield compared to the H2O2 method. Keratins obtained from the developed method exhibited typical chemical and secondary structures. Furthermore, individual keratins from four young girls were extracted by using the H2O2/TiO2/UV method, and individual keratin/PCL films were successfully fabricated within 5 h. More importantly, individual keratin films displayed superior hemostatic performance and wound healing in vivo compared to that of keratin extracted via the H2O2 method. Interestingly, the four individual keratin films exhibited similar hemostatic efficiency but distinct wound-healing properties in terms of epidermal thickness and density of skin appendages. These data indicated that the developed H2O2/TiO2/UV method is an efficient and feasible approach to extract human hair keratins, which provides an intriguing possibility to explore personalized materials for promoting wound healing.
研究领域:
材料科学
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生物医学工程
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化学工程
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组织工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Yanbo Wang,Haojie Ni,Huan Li,Jian Chen,Dequan Zhang,Linglin Fu
Microbial contamination of food is a critical public health issue worldwide, making the rapid, efficient, and safe sensing strategies for foodborne microbes in urgent need. In this work, a plasmonic microneedle (MN) array patch was developed for rapid extraction and surface-enhanced Raman spectroscopy (SERS) detection of a model bacteria, Escherichia coli ( E. coli ). A hydrophilic MN matrix was firstly prepared by crosslinking [2-(methacryloyloxy)ethyl]trimethylammonium chloride (TMA), 2-carboxyethyl acrylate (CAA), and 2-hydroxyethyl methacrylate (HEMA) in a micro-mold. Then the obtained MN patch was treated with ozone and coated with SERS-active gold nanopopcorns (GNpops) that were modified with the specific aptamer towards E. coli . The resultant plasmonic MN patch could extract E. coli from agarose simulant in 3 min and was capable of on-MN SERS analysis with a detection limit of 143 CFU/g. The proposed detection method exhibited great specificity to E. coli and was feasible for evaluating the E. coli contamination of commercial mutton samples. Moreover, the extracted bacteria could be effectively inactivated through photothermal sterilization. The work opened a new avenue for simplifying the detection procedures for microorganisms and provided a potential approach for rapid and on-site microbial monitoring through the SERS-active MN patch.
研究领域:
生物医学工程
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分析化学
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食品安全
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微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）