Food Analytical Methods
·
2023
·Vol. 16
·期 3
·633-649
·
IF 2.9
Li Fan,Wang Zhanhua,Zhu Bingqi,Xu Xiaoying,Liu Zhu
Anesthetics are widely applied in cultivation and transportation of aquatic products to reduce the metabolism of fish and avoid some stress reactions. The overdose of using anesthetics or the occurrence of their residues in the aquatic products may negatively affect human health after consumption. A rapid screening procedure and a new analytical method for the simultaneous determination of 14 anesthetics was established in this study. The compounds were extracted from fish and shrimp matrices by sodium acetate buffer solution, cleaned up via solid-phase extraction, and then were analyzed using an ultrahigh-performance liquid chromatography system coupled with a tandem quadrupole-time-of-flight mass spectrometer (UPLC-Q-TOF–MS) in a positive-ion mode. Methodological verification analyses, including linearity, precision, recovery, limit of detection (LOD), and limit of quantification (LOQ), were conducted to validate the method. The correlation coefficient of the calibration curves of each anesthetic exceeded 0.99. The average recoveries of this analysis method varied from 72.36 to 111.27% with relative standard deviations less than 5%. The detection limit of anesthetics reached 0.3–0.7 μg/kg. Agilent Mass Hunter Personal Compound Database Library (PCDL) Manager was used to establish a database of 14 anesthetics and 8 isotope internal standards, which enabled a quick screening and analysis without standard substances. The results of the spiked blank samples demonstrated reliability of the screening method with mass deviations less than 5 ppm and the scores determined by relative molecular mass, retention time, isotope ratio, and distribution all greater than 85. In addition, according to the fragmentation information of mass spectrometry, the fragmentation rules of some anesthetics were summarized. This method was validated with reliable results in quantitative and qualitative analysis and could be used as regulatory measures for anesthetics in aquatic products.
研究领域:
食品安全
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分析化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Zhuoting Liu,Chunling Wang,Xu Wang,Ann Van Schepdael
Indoleamine 2,3-dioxygenase 1 (IDO1) has been shown to play an important role in the immune escape process of tumors, and therefore is considered as a promising target for tumor immunotherapy. In this study, off-line and on-line capillary electrophoresis methods were developed for IDO1 inhibitors screening from natural product extracts. The optimized separation conditions of CE were achieved with 32 mM sodium tetraborate (pH 9.22) as background electrolyte, using a separation voltage of 21 kV. The off-line CE method was verified by the determination of enzymatic kinetic parameters and inhibitory mechanisms of two known inhibitors. A partial filling on-line CE method combined with rapid polarity switching was used for rapid screening of IDO1 inhibitors. The whole reaction and separation process was completed within 5 min. The on-line CE screening results showed that six of 18 natural products had inhibitory effect on IDO1, namely Carthamus tinctorius, Schisandra chinensis , Raisin, Coffee, Hawthorn and Radix angelicae sinensis. The results of on-line CE experiments were consistent with the off-line results, which proved the practicability and effectiveness of the method for inhibitors screening.
研究领域:
生物化学
·
药物化学
·
肿瘤学
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DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Dian Li,Yun Chen,Shangbin Kao,Canlong Yan,Tianyu Zheng,Yuan Qin,Xiumei Zhou,Xiang Shao,Biao Huang
Addressing the challenges of strong matrix interference, cumbersome procedures, and urgent demand for on-site rapid detection in the analysis of tetracycline antibiotics (TCs) residues in milk, this study aims to develop an efficient, rapid screening method based on the amplified luminescence proximity homogeneous assay (AlphaLICA) technology for the direct detection of tetracycline (TC) and other TCs residues in milk. By conjugating TC-BSA complexes to receptor microspheres and goat anti-mouse IgG antibodies to donor microspheres, this approach effectively overcomes interference from milk fat and casein, enabling wash-free, homogeneous detection. This method exhibits high sensitivity (limit of detection (LOD): 0.16 ng/mL; limit of quantification (LOQ): 0.83 ng/mL), with a linear range of 0.83-32 ng/mL. Spiked recovery rates reached 100.95%-114.78% (relative standard deviation (RSD) ≤ 8%), Intra-and inter-batch coefficients of variation (CV) ranged from 3.89%-13.24% and 4.34%-13.52%, respectively. Cross-reactivity with non-TCs antibiotics remained low (CR ≤ 10.68%). Compared to the traditional enzyme-linked immunosorbent assay (ELISA, 75 minutes, requiring washing), this method requires only 10 minutes and is wash-free. The results of actual sample testing showed high consistency with the reference method (Y = 0.7685X + 0.4623, r² = 0.952, p < 0.001). This study provides a reliable technical solution for high-throughput rapid field monitoring of TCs residues in milk.
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PubMed
高影响因子
Chemosensors
·
2021
·Vol. 9
·期 11
·314
·
IF 3.398
Yangyang Liu,Hang Gu,Jiahua He,Anqi Cui,Xiaoyi Wu,Jiaping Lai,Hui Sun
In consideration of the endocrine disrupting effects caused by the butyl paraben (BP), a portable visual sensor has been developed based on the photonic crystal and molecular imprinting technology for the rapid screen of BP in toner sample which is a type of aqueous cosmetic to soften the face skin. By integrating the self-reporting and molecular recognition properties, the molecular imprinting photonic crystal (MIPC) sensor can display obvious color changes regularly according to the concentration of BP. Based on the “color guide”, the content of BP in toner sample can be estimated directly with the naked eye. In addition, the Bragg diffraction spectrum of MIPC can red shift linearly with the increase of the concentration of BP in sample solution with correlation coefficient as 0.9968. The quantitative determination of BP can be achieved through the optical fiber spectrometer with detection limit as 0.022 mmol·L−1. With good selectivity, this MIPC film can recognize BPs against the complex sample matrix, showing a standard addition recovery of 107% for the real samples.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Ming Zhong,Hao Zhang,Yajun Ren,Yuwei Sun,Mingyue Piao,Libin Han,Mingyue Jin,Honghui Teng
Glyphosate, a widely used organophosphorus herbicide in agriculture, poses potential threats to aquatic ecosystems and human health due to its long-term environmental persistence. This study presents a spectroscopic detection system based on a competitive reaction utilizing the Ponceau 4R (P4R)-Cu2+ complex. Leveraging glyphosate's high affinity for chelating copper ions, the method enables indirect, rapid, and visual quantitative analysis of glyphosate. In the presence of glyphosate, Cu2+ preferentially binds to it, causing the release of P4R and the restoration of its characteristic absorption spectrum, resulting in a measurable absorbance change at 505 nm. Systematic optimization of buffer pH, P4R concentration, Cu2+ concentration, and their ratio established the optimal detection conditions (120 μmol L−1 P4R, 120 μmol L−1 Cu2+, 40 mmol L−1 borate buffer, pH 9.0). Under these conditions, the method exhibited a good linear relationship within the concentration range of 5.9–295.8 μmol L−1 (R2 = 0.998). The limit of quantitation (LOQ) and limit of detection (LOD) were determined to be 14.0 μmol L−1 and 4.6 μmol L−1, respectively. This method was successfully applied to the analysis of spiked surface water samples and soils collected from the upper reaches of the Liao River, yielding average recoveries of 98.7–107.6% with relative standard deviations (RSDs) below 5%. Interference tests indicated that citric acid interference was manageable (recovery: 88.9%) when its concentration ratio to glyphosate was ≤2 : 1, while tartaric acid and oxalic acid required masking strategies to suppress their interference. This method, characterized by its simplicity, rapid response, and low instrumental dependence, provides reliable technical support for the rapid screening of glyphosate in environmental samples.
研究领域:
环境科学
·
分析化学
·
光谱学
·
水污染
·
除草剂检测
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Wenyan Shi,Xinming Huo,Xinyue Ding,Peiyuan Zhu,Yutong Wan,Xinqiong Lu,Rui Feng,Quan Yu,Xiaohao Wang
With the expansion of the functional food market, the qualification assessment of these products has become a major challenge, and efficient analytical tools are urgently needed. Here, a miniature mass spectrometer (MS) with self-aspiration capillary electrospray ionization (SACESI) source and ion trap analyzer was developed for rapid screening of various illegally added drugs in functional foods. No chromatographic separation was required, but a simplified two-step pretreatment method was developed to reduce the operational procedures and time consumption of the entire analysis. SACESI source uses capillary action to drive solution injection, which utilizes a simple structure and convenient operation to constitute a kind of disposable MS detection solution. To achieve accurate and automatic identification, an intelligent recognition algorithm with steps of spectrum preprocessing, characteristic peak matching, and support vector machine learning was constructed. The relative accuracy of rapid screening of 31 suspicious drugs in various samples is up to 99.78%. It achieves 100% correct identification for the 55 batches of actual samples captured by on-site inspection, which demonstrates the feasibility of the proposed analytical system and strategy in food safety applications.
研究领域:
食品安全
·
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Beining Chen,Zhengshuang Yang,Xiaolei Qu,Shourong Zheng,Daqiang Yin,Heyun Fu
The extensive production and large-scale use of perfluoroalkyl substances (PFASs) have raised their presence in aquatic environments worldwide. Thus, the facile and reliable screening of PFASs in aqueous systems is of great significance. Herein, we designed a novel fluorescent sensor array for the rapid screening and discrimination of multiple PFASs in water. The sensor array comprised three highly stable zirconium porphyrinic luminescent metal–organic frameworks (i.e., PCNs) with different topological structures. The sensing mechanism was based on the static fluorescence quenching of PCNs by PFASs upon their adsorptive interactions. The fluorescence response patterns were characteristic for each PFAS because of their different adsorption affinities toward different PCNs. Through the interpretation of response patterns by statistical methods, the proposed PCN array successfully discriminated six different kinds of PFASs, each PFAS at different concentrations and PFAS mixtures at different molar ratios. The practicability of this array was further verified by effectively discriminating PFASs in two real water samples. Remarkably, the PCN sensors exhibited a very short response time toward PFASs (within 10 s) due to the ordered pore structure allowing fast PFAS diffusion. This study not only provides a facile method for rapid PFAS screening in waters but also broadens the application of luminescent metal–organic frameworks and array techniques in sensing fields.
研究领域:
环境科学
·
分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Zhoubing Huang,Liwei He,Huizhen Li,Junbo Zhao,Tianyang Chen,Ziang Feng,Yangyang Li,Jing You
Effect-directed analysis (EDA) has been increasingly used for screening toxic contaminants in the environment, but conventional EDA procedures are often time-consuming and labor-extensive. This challenges the use of EDA for toxicant identification in the scenarios when quick answers are demanded. Herein, a solid phase microextraction ligand fishing (SPME-LF) strategy has been proposed as a rapid EDA approach for identifying acetylcholinesterase (AChE) active compounds in water. The feasibility of ligand fishing techniques for screening AChE active chemicals from environmental mixtures was first verified by a membrane separation method. Then, SPME fibers were prepared through self-assembly of boronic acid groups with AChE via co-bonding and applied for SPME-LF. As AChE coated SPME fibers selectively enriched AChE-active compounds from water, comparing sorbing compounds by the SPME fibers with and without AChE coating can quickly distinguish AChE toxicants in mixtures. Compared with conventional EDA, SPME-LF does not require repeating sample separations and bioassays, endowing SPME-LF with the merits of low-cost, labor-saving, and user-friendly. It is believed that cost-efficient and easy-to-use SPME-LF strategy can potentially be a rapid EDA method for screening receptor-specific toxicants in aquatic environment, especially applicable in time-sensitive screening.
研究领域:
环境科学
·
分析化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Journal of Spectroscopy
·
2016
·
IF 0.814
Tong Wu,Hui Chen,Zan Lin,Chao Tan
Melamine is a nitrogen-rich substance and has been illegally used to increase the apparent protein content in food products such as milk. Therefore, it is imperative to develop sensitive and reliable analytical methods to determine melamine in human foods. Current analytical methods for melamine are mainly chromatography-based methods, which are time-consuming and expensive and require complex pretreatment and well-trained technicians. The present paper investigated the feasibility of using near-infrared (NIR) spectroscopy and chemometrics for identifying and quantifying melamine in liquor milk. A total of 75 samples were prepared. Uninformative variable elimination-partial least square (UVE-PLS) and partial least squares-discriminant analysis (PLS-DA) were used to construct quantitative and qualitative models, respectively. Based on the ratio of performance to standard deviate (RPD), UVE-PLS model with 3 components resulted in a better solution. The PLS-DA model achieved an accuracy of 100% and outperformed the optimal reference model of soft independent modeling of class analogy (SIMCA). Such a method can serve as a potential tool for rapid screening of melamine in milk products.
研究领域:
食品科学
·
分析化学
·
光谱学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Mengting Huang,Jianna Yu,Xiuqing Zheng,Jiaxu Liu,Peixuan Huang,Guoxing Jing,Wen Liu,Wenshan Li,Wenjie Liu
The detection of potent carcinogenic nitrosamine impurities in pharmaceutical products has attracted significant regulatory attention in recent years, and advanced analytical methods are needed to accurately quantify nitrosamines in pharmaceutical products. In this study, a headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) method was proposed and validated, which addresses two key challenges: achieving the sub-ppb sensitivity required for low-concentration nitrosamine analysis, and reducing matrix interferences in extended-release formulations. Seven investigated nitrosamines showed good linearity (R 2 >0.99) in the range of 0.5-100 ng/mL, with limits of detection (LOD) of 0.05-0.51 ng/mL and limits of quantification (LOQ) of 0.16-1.70 ng/mL. Precision studies (intra-day relative standard deviation RSD<5%, inter-day RSD<13%) and satisfactory spiked recoveries (84.31%-106.60%) in active pharmaceutical ingredient matrices confirmed the robustness of this method. The developed method eliminates the need for complex extraction and pre-concentration procedures, requires minimal sample preparation and enables rapid gas chromatography separation.
研究领域:
药物分析
·
药物质量控制
·
毒理学
·
分析化学
·
药剂学
·
药物安全
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DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 12
·2883-2888
·
IF 3.864
Hui Ouyang,Wenwen Wang,Qi Shu,Zhifeng Fu
A novel immunochromatographic assay (ICA) using a dual-readout signal probe was developed for multiplexed detection of pesticide residues by adopting methyl parathion and fenpropathrin as model analytes. Luminol-reduced Au nanoparticles (LRAuNPs) were synthesized and utilized in the proposed ICA platform as a colorimetric/chemiluminescent (CL) dual-readout probe. The methyl parathion antibody and fenpropathrin antibody were tagged with the prepared LRAuNPs to conduct spatially-resolved multiplexed detection. After the occurrence of two immunoreactions on the test strip, the probes were captured by the immobilized antigens on the two test zones. The red color resulting from the accumulation of captured LRAuNPs was adopted as the visual and semi-quantitative readout. For the sensitive quantitative detection of the analytes, the CL signals caused by the luminophore in the LRAuNPs were collected after triggering the luminol-H2O2 CL reaction. Under the optimal conditions, the detection limits for methyl parathion and fenpropathrin were 0.17 ng mL−1 and 0.10 ng mL−1 (S/N = 3), respectively. The whole procedure for ICA was completed within 15 min. The present ICA protocol was successfully applied for detection of pesticide residues in spiked traditional Chinese medicine samples. This dual-readout ICA platform showed merits such as low cost, time efficiency, easy operation and high sensitivity. Its application potential has been demonstrated in the rapid screening and field detection of multiple pesticide residues.
研究领域:
分析化学
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环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Sujia Jiao,Xiaoxia Xie,Zhenyun He,Zhichang Sun,Zheming Wang,Sihang Zhang,Hongmei Cao,Bruce D. Hammock,Xing Liu
The common food allergy crustacean tropomyosin (TM) poses a significant food safety challenge, which requires rapid and sensitive methods for screening TM in food. Herein, the variable new antigen receptor (VNAR) single-domain antibodies specific for the crustacean TM were isolated from a naïve phage-displayed shark VNAR library. Subsequently, a lateral flow immunochromatographic assay (LFIA) based on the gold nanoparticle-labeled phage-displayed shark VNAR (AuNPs@PSV) probe was developed for the detection of TM in food. The AuNPs@PSV-LFIA took 15 min for one test and had a visual limit of detection (vLOD) of 0.1 μg/mL and an instrumental LOD of 0.02 μg/mL. Good selectivity, accuracy, precision, and stability were confirmed for the AuNPs@PSV-LFIA. Moreover, the test results of 21 commercially available food products consisted of the allergen labels and were validated by a commercial ELISA kit. Therefore, this work demonstrated the great potential of VNAR for detecting TM in food by LFIA.
研究领域:
食品科学
·
免疫学
·
生物技术
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）