Small
·
2025
·
IF 12.1
Xinxin Liu,Tao Liu,Xueli Yuan,Jing Zhang,Mengjiao Zhu,Wei Zhang,Zhenyu Chu,Wanqin Jin
Viral hepatitis is a highly infectious disease caused by various hepatitis viruses that, thus far, have no cure. In early viral invasion, a low viral load in the blood makes early detection difficult unless the symptoms are severe. Herein, a new ultrasensitive and ultrafast hepatitis virus detection strategy is proposed by constructing a dual-surface-activated nanotube that enables recognition probes to not only bind to the outer surface of the nanotube but also penetrate and lock onto its inner surface. This approach significantly enhances both the specific surface area and number of active sites, thereby improving the capture efficiency for traces of hepatitis viral DNA, such as the B type (HBV-DNA). Compared with current DNA detection methods, this approach eliminates the additional amplification steps to achieve an ultralow detection limit of 10 −18 m , advancing the available detection time as early as possible. Using human serum as a sample, this technique enables the precise and quantitative reporting of HBV-DNA within only 15 min and overcomes the interference of usual proteins and other viral DNA. This technique provides new inspiration for quick and early hepatitis diagnosis for in-hospital and non-hospital patients without bulky instruments and complex operations.
研究领域:
生物技术
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yonggan Xue,Jimei Chi,Bobin Ning,Lijun Cheng,Wenchen Wang,Qingyu Meng,Liang Huang,Mu Ke,Sen Li,Meng Su,Baoqing Jia
Due to non-specific signs in the early stage of colorectal cancers, most patients result in a low early diagnosis rate. Survivin protein is upregulated in many malignant tumors, making it an ideal diagnostic and therapeutic target for colorectal cancers. However, the survivin protein is located in the cell nucleus, which needs the probe to penetrate the complex nuclear membrane structure for targeting and recognizing. Here, we have developed a sub-100 nm perovskite-based fluorescent probe modified with the survivin antibody, which can freely target survivin protein through the nuclear membrane. Polyvinyl pyrrolidone (PVP) coated perovskite crystals are obtained by the supersaturated crystallization, which keep the probe with great fluorescence intensity and resistance of water and oxygen. We have established colorectal cancer cell lines and colorectal cancer tissues to test the feasibility and efficacy of the probe. After investigating the fluorescence intensity values of 20 probing samples, the average fluorescence intensity of colorectal cancer sections is 8.7 times higher than that of paracancerous tissues. Our work demonstrates that the survivin can be an ideal fluorescence target for rapid diagnosis of colorectal cancer, which also provides a research foundation for fluorescence-guided surgery and nano-therapy for targeting the survivin protein.
研究领域:
肿瘤学
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分子生物学
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医学影像学
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Xi Su,Yi Xu,Huazhou Zhao,Shunbo Li,Li Chen
A microfluidic SERS chip integrated with blood separation and in-situ detection was designed and fabricated for the rapid detection of clinical blood samples. Each functional unit in the microfluidic SERS chip consist of separation-decantation cavity based on centrifugal separation principle, mixing channels and SERS detection chamber built with integrated nano-Au on Ag film microstructure. The serum creatinine was selected as a typical sample to demonstrate the capability of microfluidic SERS chip. It was found that the creatinine SERS characteristic peaks at 678 cm −1 can be effectively identified and the detection limit could be as low as 4.42 × 10 −3 μmol mL −1 in water. The blood samples were also tested in microfluidic SERS chip. The whole separation and test process could be completed within 2 min, which is a significant improvement in the field of creatinine detection. The whole blood of six cases clinical blood samples were also tested, and the results were consistent with the enzymatic results. The developed microfluidic SERS chip has advantages including reduction of the required quantity of blood sample, reusable and easy to operate. It is expected to provide a new method for rapid diagnostics .
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Xin Yue,Yu Huang,Yu Zhang,Hui Ouyang,Jianping Xie,Zhifeng Fu
Mycobacterium tuberculosis ( M. tuberculosis ), the causative agent of tuberculosis, ranks one of the most dangerous pathogens for its large deaths toll. Due to its characteristic extremely slow growth, the conventional culture-based protocol cannot meet the requirement for the efficient diagnosis of M. tuberculosis -induced tuberculosis. With our previously isolated mycobacteriophage SWU1, we tried to develop a mycobacteriophage-based protocol for detecting Mycobacterium genus. In this work, Mycobacterium smegmatis ( M. smegmatis ) was used as a model due to its similar physiological features as pathogenic M. tuberculosis , much faster growth and nonpathogenic property. Mycobacteriophage SWU1-functionalized magnetic particles (SWU1-MPs) were used as highly efficient separation carriers for the viable host M. smegmatis . After a replication cycle of approximate 60 min, the cells of M. smegmatis were disrupted by the progeny mycobacteriophages to release intracellular adenosine triphosphate (ATP). The bioluminescent (BL) signal of released ATP was collected to quantitate the amount of M. smegmatis . For the developed protocol, the detection range is 5.0 × 10 2 to 5.0 × 10 5 CFU mL −1 , and the detection limit is 3.8 × 10 2 CFU mL −1 ( S / N = 3). Furthermore, the protocol can exclude the potential interference of 3 non-pathogenic mycobacteria and 6 other bacterial species. It has been successfully applied to quantitate M. smegmatis in human urine, human saliva, and human serum. The results demonstrate its application potential for a simple, fast, and specific diagnosis of M. tuberculosis infection.
研究领域:
医学微生物学
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分子诊断学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Xiaoqin Li,Huizi Lin,Xuwei Chen,Fenqiang Luo,Rong Zhang,Xiaoyan Deng,Youxiu Lin,Dianping Tang,Wenqiang Lai
Colorimetric immunoassays are widely used for biomarker detection, offering advantages of simplicity, sensitivity, and cost-effectiveness. Recent advancements focus on improving the catalytic activity of nanozymes for enhancing the sensitivity and accuracy of such assays. Bimetallic CuCo Prussian blue analog (CuCo PBA) has emerged as promising candidates due to their excellent peroxidase-like activity. However, their instant synthesis and integration into immunoassays for the rapid detection of biomarkers like carcinoembryonic antigen (CEA) remain underexplored. This study presents an innovative approach using CuCo PBA nanozymes for colorimetric immunoassays with immediate generation and application. In this study, CuCo PBA nanozymes were synthesized instantly by reacting Cu 2+ with K 3 [Co(CN) 6 ] (<1 min), and their peroxidase-like activity was exploited for a colorimetric immunoassay system targeting CEA. The system demonstrated a clear blue color change upon the reaction of CuCo PBA with H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB), enabling sensitive detection. The assay was optimized for various parameters, including pH, temperature, and material ratio. A linear response was obtained for CEA detection over a concentration range of 0.05–60 ng/mL with a limit of detection (LOD) of 22 pg/mL. The integration of glucose oxidase (GOx) mediated the generation of H 2 O 2 , triggering the colorimetric reaction. This instantaneous CuCo PBA-based system effectively detected CEA in human serum samples, highlighting its potential for rapid diagnostic applications. This work introduces a novel approach for rapid and sensitive colorimetric immunoassays using CuCo PBA nanozymes that are synthesized on-demand and immediately applied. The system allows for efficient CEA detection with an exceptionally low detection limit, offering great potential for clinical diagnostics. The instant generation and application of CuCo PBA nanozymes in immunoassays represent a significant advancement in point-of-care testing technologies.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 25
·6945-6956
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IF 3.125
Kumar Saroj,Kumar Dileep,Ahirwar Rajesh,Nahar Pradip
Bioconjugation and functionalization of polymer surfaces are two major tasks in materials chemistry which are accomplished using a variety of coupling agents. Immobilization of biomolecules onto polymer surfaces and the construction of bioconjugates are essential requirements of many biochemical assays and chemical syntheses. Different linkers with a variety of functional groups are used for these purposes. Among them, the benzophenones, aryldiazirines, and arylazides represent the most commonly used photolinker to produce the desired chemical linkage upon their photo-irradiation. In this review, we describe the versatile applications of 4-fluoro-3-nitrophenyl azide, one of the oldest photolinkers used for photoaffinity labeling in the late 1960s. Surprisingly, this photolinker, historically known as 1-fluoro-2-nitro-4-azidobenzene (FNAB), has remained unexplored for a long time because of apprehension that FNAB forms ring-expanded dehydroazepine as a major product and hence cannot activate an inert polymer. The first evidence of photochemical activation of an inert surface by FNAB through nitrene insertion reaction was reported in 2001, and the FNAB-activated surface was found to conjugate a biomolecule without any catalyst, reagent, or modification. FNAB has distinct advantages over perfluorophenyl azide derivatives, which are contemporary nitrene-generating photolinkers, because of its simple, single-step preparation and ease of thermochemical and photochemical reactions with versatile polymers and biomolecules. Covering these aspects, the present review highlights the flexible chemistry of FNAB and its applications in the field of surface engineering, immobilization of biomolecules such as antibodies, enzymes, cells, carbohydrates, oligonucleotides, and DNA aptamers, and rapid diagnostics.
An overview of the FNAB-engineered activated polymer surfaces for covalent ligation of versatile biomolecules
研究领域:
生物化学
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材料化学
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医学诊断
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 125
·103557-103562
·
IF 3.84
Miao Chen,Liya Rong,Xiaoqing Chen
It is well known that Cu(II) can coordinate with L-alanine (Cu–Ala), which can be destroyed through the addition of glutamic-pyruvic transaminase (GPT) since GPT can effectively catalyze the conversion of L-alanine into keto-acetic acid. As a result, the free Cu(II) ion can combine with bovine serum albumin (BSA) and in turn quench the fluorescence of BSA. In this context, a simple and sensitive GPT activity detection via fluorescence quenching method has been developed. The fluorescence intensity of the system shows a linear relationship with the GPT concentration in the range of 5 and 400 U L−1 with a detection limit down to 3 U L−1 (S/N = 3). Avoiding any labels or complicated operations, this cost-effective and convenient method holds the potential for the rapid diagnosis of GPT-related diseases.
研究领域:
分析化学
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生物化学
·
医学诊断
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产品关联
DOI
高影响因子
ANALYST
·
2025
·
IF 3.3
Chenyao Lin,Qiaoping Wu,Menglu Mao,Xuedan Qiu,Chuanfan Ding,Yinghua Yan,Dumei Ma,Qingcao Li
The global rise of carbapenemase-producing Klebsiella pneumoniae (CPKP) poses a major threat to public health due to its high morbidity and mortality rates, driven largely by the production of carbapenemase enzymes. Rapid and accurate detection of carbapenemase activity is crucial for guiding appropriate antimicrobial therapy and controlling nosocomial transmission. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) enables direct detection of carbapenem hydrolysis but is currently limited by the drawbacks of conventional organic matrices, such as high background interference, toxic solvents, and poor reproducibility. In this study, we report a novel nanocomposite matrix, GNP@Zn–Cu/MOF@Au, synthesized via a green, aqueous-phase method without toxic reagents or extreme conditions. This heterostructured material exhibited a large mesoporous surface area, excellent UV absorption, and thermal responsiveness, which enhanced signal intensity, reproducibility, and analytical sensitivity, significantly improving MALDI-TOF MS performance. When applied to the identification of carbapenemase activity in clinical CPKP isolates, the matrix achieved 100% sensitivity and specificity after 1 hour of incubation and 97.3% sensitivity after just 15 minutes. Notably, KPC-2-producing strains were detected with full sensitivity within 15 minutes, while extended incubation enabled reliable identification of low-activity enzymes such as OXA-48. These findings demonstrate the strong potential of GNP@Zn–Cu/MOF@Au as a reliable and sustainable alternative to conventional matrices for rapid and accurate detection of carbapenemase activity in clinical diagnostics.
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yuexi Kang,Wenlong Ren,Qing Fu,Ruotong Yang,Jie Li,Xin Gao,Fengjiao Chen,Tao Zeng,Li Xu,Wei Cheng,Juan Li
Urinary tract infections (UTIs) are among the most common infectious diseases worldwide, characterized by high recurrence rates and increasing antibiotic resistance. Currently, urine culture remains the gold standard for pathogen diagnosis, yet it suffers from low sensitivity and prolonged turnaround times (16–18 h). To address these limitations, we developed a rapid and interference-free phosphorescence biosensor based on persistent luminescent nanoparticles (PLNPs), enabling accurate bacterial detection in complex clinical samples without autofluorescence or exogenous interference. Polymyxin B was conjugated with both PLNPs and magnetic nanoparticles (MNPs), forming a sandwich-structured detection system: MNPs facilitate magnetic enrichment and separation of pathogens, while PLNPs provide a stable phosphorescent signal for quantification. Using Escherichia coli as a model pathogen, the assay achieved a detection limit of 13 CFU mL −1 within 15 min. Clinical validation using 30 urine specimens showed excellent correlation with standard culture methods. This PLNP-based biosensor not only offers a promising alternative for rapid UTI diagnosis but also paves the way for personalized antimicrobial therapy management.
研究领域:
生物传感器
·
传染病学
·
纳米医学
·
尿路感染
·
抗菌素耐药性
✓ 已验证
产品关联
DOI
高影响因子
InfoMat
·
2023
·
IF 22.7
Dongdong Wu,Jimei Chi,Junzhen Fan,Lijun Cheng,Xuning Wang,Xu Yang,Meng Zhang,Zewei Lian,Zengqi Huang,Huadong Wang,Hongfei Xie,Sisi Chen,Qi Pan,Zeying Zhang,Bingda Chen,Guochen Sun,Bainan Xu,Meng Su,Yanlin Song
Histopathology plays a great role in diagnosing various diseases, which is considered as a golden standard for tumor identification. The tissue constituents must be stained by visible labels for microscopic analysis by medical experts. However, this process is time-consuming, labor-intensive, and expensive, which requires rapid pathological approaches for diagnosis in the operating room. Here, we present an easy-to-process and high-performance perovskite biological probes for rapid and visual pathological diagnosis of glioma. Perovskite quantum dots can be encapsulated by the copolymer into nanocrystals (PNCs) with a diameter of 100 nm, which is modified with chlorotoxin to achieve the specific recognition of glioma. Benefiting from the super photoluminescence quantum yield (above 93%) of EVA@PNCs aqueous solution, the glioma can be clearly imaged and captured via a smartphone under the excitation of a handheld UV lamp. To demonstrate the visualization and efficiency of PNC probes, different malignant grades of brain tumor sections can be distinguished in no more than 5 min. This strategy provides a general auxiliary diagnosis platform for achieving the histopathology analysis near the operating bed, which is currently not feasible with standard histochemical staining methods.
研究领域:
医学影像
·
病理学
·
生物技术
·
材料科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Chunxia Chen,Shujie Duan,Jianing Ji,Mengli Wu,Zhan Yang,Mengyang Cai,Menghan Xue,Lanju Wang,Rui Chen,Sima Yaron,Keying Guo,Sergio Benardini,Zhizeng Wang,Yang Luo
Conventional point-of-care lateral flow immunoassays are characterized by an antibody-tagged probe irregular coupling that can limit sensitivity and require a long assay’s time. We synthesized polyethylene glycol-modified selenium nanoparticles (PEG-SeNPs) by template method and developed a strategy to set antibody probes targeted and orderly by using PEG-SeNPs. Synthesized PEG-SeNPs with high stability could immobilize antibodies in the “stand-up” orientation, resulting in a faster detection time of less than 1 min by direct observer visualization without instruments or equipment. Results showed that SARS-CoV-2 antibody could be systematically structured on the chip, resulting in a detection limit of 10 pg/mL, significantly higher than conventional chips. The new device has been validated on 192 clinical samples and we found 100% negative coincidence, 93.94% positive coincidence, and 95.83% overall coincidence with reverse transcriptional PCR test. The orderly arrayed probe’s stability allowed to detect throat swabs, saliva, serum, fingertip blood samples, and mutant strains without cross-reactivity with common respiratory viruses or pathogenic strains, demonstrating promising potential for a universal colorimetric platform for ultrafast field-deployable diagnostics.
研究领域:
生物医学工程
·
纳米技术
·
病毒学
·
诊断医学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Rujing Sun,Xin Li,Min Jiang,Yelongzi Cao,Yaqian Yang,Hao Wen,Zhenghan Shi,Chaobo Dai,Hao Liang,Qingjun Liu
Seasonal outbreaks of respiratory infections place a significant burden on hospital-centered diagnostic and treatment systems. Co-infections involving both bacterial and viral pathogens further complicate infection assessment, highlighting the need for rapid and accurate diagnostic tools. In this work, we present a ready-to-use, portable resonant sensing system capable of rapidly and quantitatively detecting key coinfection biomarkers in saliva, specifically C-reactive protein (CRP) and respiratory syncytial virus (RSV) fusion protein. The sensor operates via competitive immune binding within a hydrogel-based resonant structure, where biomarker binding induces shifts in resonant frequency that are wirelessly detected. To preserve antibody activity and hydrogel mechanical integrity, we developed an integrated, ready-to-use sensor system optimized for ultrasensitive detection. This platform enables analysis of unprocessed saliva samples within 5 min, achieving low limits of detection of 24 fg/mL for CRP and 31 fg/mL for RSV fusion protein. Quantitative analysis of patient samples demonstrated strong concordance with both enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) results. This portable sensing platform offers a promising solution for point-of-care coinfection diagnostics, supporting real-time infection monitoring and potentially easing the burden on healthcare systems.
研究领域:
生物医学工程
·
传染病诊断
·
生物传感器
·
微流控技术
·
免疫学
·
即时检测
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）