NEUROSCIENCE
·
2017
·
IF 3.277
Tanzeel Huma,Xintian Hu,Yuanye Ma,Andrew Willden,Joshua Rizak,Muhammad Shahab,Zhengbo Wang
Embryonic stem cells (ESCs) have enormous potential as novel cell-based therapies, but their effectiveness depends on stem cell differentiation and specific signaling regulators, which remain poorly understood. In this study, a kisspeptin peptide (KP-10) was used at different dosages to determine whether rhesus macaque-derived tau GFP-Lyon ES cells underwent kisspeptin-specific neuronal differentiation. It was found that KP-10 exhibited an anti-proliferative effect on the cells and led to morphological changes and cellular differentiation consistent with neuronal stem cell (NSC) development. The cells differentiated into Gonadotrophin Releasing Hormone (GnRH) neuronal-like cell types in response to the KP-10 treatment. There has been a previously observed connection between kisspeptin signaling, GnRH neurons and their dysfunction found in congenital disorders like idiopathic hypogonadotropic hypogonadism (IHH). Although therapeutics are a still a far-off goal, the formation and development of GnRH-positive neuronal-like cells following the application of KP-10 to Lyon NSC cells opens the door for future NSC-based therapies to treat specific reproductive disorders.
研究领域:
神经科学
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生殖生物学
·
干细胞研究
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Guizhu Wu,Lu Gao,Shaoming Zhang,Daolin Du,Yonglai Xue
Copper oxide nanoparticles (CuO NPs) were regarded as the versatile materials in daily life and the in-depth evaluation of their biological effects is of great concern. Herein the female and male zebrafishes were chosen as the model animals to analyze the reproductive toxicity caused by CuO NPs at low concentration (10, 50 and 100 μg/L) After 20-days exposure, the structure of zebrafish ovary and testis were impaired. Moreover, the contents of 17β-estradiol (E2) in both females and males were increased, while the contents of testosterone (T) were decreased, indicating the imbalanced sex hormones caused by CuO NPs. The expression of genes along the hypothalamic pituitary-gonad (HPG) axis, were examined with quantitative real-time PCR to further evaluate the toxic mechanisms. Meanwhile, the levels of erα/er2β and cyp19a in female zebrafishes and erα/er2β , lhr , hmgra/hmgrb , 3βhsd and 17βhsd in male zebrafishes were obviously up-regulated. While, the level of αr was obviously down-regulated in female and male zebrafishes. Thus, the obtained data uncovered that long-term exposure of CuO NPs with low dose could trigger the endocrine disorder, resulting in the disturbance of E2 and T level, inhibition of gonad development, and alteration of HPG axis genes. In brief, this study enriched the toxicological data of NPs on aquatic vertebrates and provided the theoretical support for assessing the environmental safety of NPs.
研究领域:
环境毒理学
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水生生物学
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内分泌学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2021
·
IF 4.294
Shuwen Tan,Yi Zhou,Haiquan Zhao,Jinhua Wu,Hui Yu,Yin Yang,Yalan Yang,Huabin Zhao,Hua Li
XX sex reversal, also called XX disorders of sex development (XX-DSD), is a condition affecting the development of the gonads or genitalia, and is relatively common in pigs. However, its genetic etiology and transcriptional regulation mechanism in the hypothalamic-pituitary-gonadal axis (HPGA) remain mostly unknown. XX-DSD ( SRY -negative) pigs and normal sows were selected by external genitalia observation. The hypothalamus, which is the integrated center of the HPGA was sampled for whole-transcriptome RNA-seq. The role of DEmiRNA was validated by its overexpression and knockdown in vitro. A total of 1,258 lncRNAs, 1,086 mRNAs, and 61 microRNAs differentially expressed in XX-DSD pigs compared with normal female pigs. Genes in the hormone biosynthesis and secretion pathway significantly up-regulated, and the up-regulation of GNRH1 , KISS1 and AVP may associate with the abnormal secretion of GnRH. We also predicted the lncRNA-miRNA-mRNA co-expression triplets and constructed three competing endogenous RNA (ceRNA) potentially associated with XX-DSD. Functional enrichment studies suggested that TCONS_00340886 , TCONS_00000204 and miR-181a related to GnRH secretion. Further, miR-181a inhibitor up-regulated GNRH1 , PAK6 , and CAMK4 in the GT1-7 cells. Conversely, transfection of miR-181a mimics obtained the opposite trends. The expression levels of FSHR , LHR , ESR1 and ESR2 were significantly higher in XX-DSD gondas than those in normal sows. Taken together, we proposed that the balance of endocrine had broken in XX-DSD pigs. The current study is the first to examine the transcriptomic profile in the hypothalamus of XX-DSD pigs. It provides new insight into coding and non-coding RNAs that may be associated with DSD in pigs.
研究领域:
遗传学
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分子生物学
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动物科学
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内分泌学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 3
·1-18
·
IF 2.4
Kong Lingnan,Abulizi Abudula,Cong Jingjing,Meng Weihan,Chang Yaqing,Sun Zhihui
The cytochrome P4503A ( CYP3A ) subfamily has been well characterized in vertebrates, where it plays a pivotal role in xenobiotic detoxification and the regulation of steroid hormone levels. However, their molecular features and functions in invertebrates remain poorly understood. The sea cucumber ( Apostichopus japonicus ) is not only a commercially significant mariculture species but also an excellent model for studying organ development and evolutionary biology. In this study, we identified seven CYP3A genes in A. japonicus using gonadal transcriptomic data. These genes exhibited ubiquitous expression across adult tissues, with significantly higher levels in ovarian tissues compared to testes tissues. Protein sequence analysis revealed that the catalytic residues Ala 306 -Thr 310 -Cys 437 , which are critical for aromatase activity, are highly conserved. This suggests that CYP3A proteins may mediate aromatization-like reactions similar to CYP19A in vertebrates. Additionally, dietary administration of the aromatase inhibitor letrozole led to histological changes in the testes and a reduction in the diameter of large oocytes in the ovaries. These findings provide valuable insights into the molecular mechanisms underlying gonadal development in sea cucumbers and lay the groundwork for future research on the artificial induction of sex reversal in echinoderms.
研究领域:
分子生物学
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海洋生物学
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进化生物学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2017
·
IF 6.198
Xiaoqing Ye,Wuye Pan,Shilin Zhao,Yuehao Zhao,Yimin Zhu,Jing Liu,Weiping Liu
Although an acceleration of male pubertal development has been observed, precisely which endocrine-disrupting chemicals (EDCs) might contribute to the advancing onset of puberty in boys remains unclear. Here, pyrethroids, a class of widely used insecticides that have been considered as EDCs, are proposed as new environmental risk factors. In this study, 463 boys at the age of 9–16 years old were recruited in Hangzhou, Zhejiang, China. The common metabolites of pyrethroids, 3-phenoxybenzoic acid (3-PBA), and 4-fluoro-3-phenoxybenzoic acid (4-F-3-PBA), as well as gonadotropins, including luteinizing hormone (LH) and follicle-stimulating hormone (FSH), were analyzed in urine samples. Pubertal development was assessed based on Tanner stages and testicular volume (TV). A positive association between 3-PBA and gonadotropins was found (p < 0.001), in which a 10% increase in 3-PBA was associated with a 2.4% and 2.9% increase in LH and FSH, respectively. Higher urinary levels of 3-PBA in boys were associated with 275% and 280% increase in the risk of being genitalia stage 3 (G3) and G4, respectively (p < 0.05). There was a significant (132%) induction in odd of being TV 12–19 mL with increasing 3-PBA concentration compared to being in TV < 4 mL (p < 0.05). For the first time to our knowledge, this work reports on an association of increased pyrethroid exposure with elevated gonadotropins levels and earlier pubertal development in boys.
研究领域:
环境科学
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内分泌学
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公共卫生
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DOI
PubMed
高影响因子
Animals
·
2025
·Vol. 15
·期 6
·870
·
IF 2.7
Bai Liufu,Qiyao Su,Kunhao Hong,Jie Wei,Yakun Wang,Zhiqiang Han,Lingyun Yu
Simple Summary17α-methyltestosterone (MT) is known to suppress ovaries and induce spermatogenesis; yet, its effects in crustaceans remain underexplored. This study investigates the impact of dietary MT on gonad development and intestinal microbiota in juvenileMacrobrachium rosenbergiithrough sex ratio determination, histological observation, the expression of reproductive development-related genes, and an analysis of the intestinal microbiota structure. The results showed that an. optimal MT concentration and treatment duration promoted gonadal differentiation and growth. However, excessive MT concentrations inhibited male gonad development and slowed growth rates. MT supplementation significantly increased microbial abundance and altered the intestinal microbial community structure. These findings clarify the effects of adding MT to feed on the reproductive development and intestinal microbiota of juvenileM. rosenbergii, providing a reference for MT application in crustaceans.17α-methyltestosterone (MT) is known for its ability to suppress ovaries and induce spermatogenesis; yet, its effects in crustaceans are underexplored. This study investigates the impact of varying MT concentrations on the gonadal development and intestinal microbiota of juvenileMacrobrachium rosenbergii. Feeds containing different MT doses were provided, and the sex ratios, histological observations, reproductive gene expression, and intestinal microbial composition were analyzed. The results revealed short-term feeding (60 days) of 1000 mg/kg MT resulted in the highest male ratio, while long-term feeding (150 days) of 500 mg/kg MT achieved the same outcome. Conversely, long-term feeding of 1500 mg/kg MT led to the lowest male ratio and retarded male germ cell development. An intestinal microbiota analysis showed that MT supplementation significantly increased microbial abundance and altered the intestinal microbial community structure. Additionally, MT suppressed the expression of female reproductive-related genes. This study provides insights into the effects of MT on reproductive development and gut microbiota in juvenile prawns, offering a valuable reference for the application of MT in crustacean aquaculture.
研究领域:
水产养殖学
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分子生物学
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微生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 84
·80383-80389
·
IF 3.289
Minglu Hao,Rutao Liu
As an emerging nanomaterial, quantum dots (QDs) have received enormous attention for their excellent photophysical properties. However, their biotoxicity restricts the application and development of quantum dots. To evaluate the toxicity of quantum dots at the molecular level, the effects of quantum dots on human chorionic gonadotropin (hCG) were explored using spectroscopic techniques and isothermal titration calorimetry (ITC). It was demonstrated that MPA (mercaptopropionic acid)-CdTe QDs interacted with hCG through hydrogen bonds and van der Waals interactions, causing a decrement in the α-helix of the protein and an unfolding process. The conformational changes of hCG lead to the damage of hydrogen bonding networks and the exposure of tyrosine residues to the surface of hCG, which might be the reason why the hCG fluorescence intensity increased and the bioactivity decreased with the increasing concentration of MPA-CdTe QDs.
研究领域:
生物化学
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纳米生物学
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毒理学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Jia-Nan Yan,Yu-Qiao Wang,Xin-Yu Jiang,Jia-Run Han,Yi-Nan Du,Jin-Feng Pan,Hai-Tao Wu
The electrostatic complex coacervation between scallop Patinopecten. yessoensis male gonad hydrolysates (SMGHs) and κ -carrageenan ( κ -C) were monitored by using turbidimetry at various pH (1–12) and biopolymer mixing ratio (9:1–1:9). The pH c exhibited ratio-independent behavior, and pH φ1 , pH max exhibited ratio-dependent behavior, respectively. The decreasing ratio enhanced the gel strength of SMGHs/ κ -C at higher pH while inversely at lower pH, ascribing to more SMGHs aggregates and stronger neutralization between positively charged patches in SMGHs and κ -C at lower pH and higher ratio. Moreover, SMGHs/ κ -C gel at acid condition exhibited lower relaxation times ( T 21 and T 23 ). Furthermore, the rheological and relaxation time T 2 data were well associated with microscopy images which indicated that SMGHs/ κ -C gel showed a well-distributed network structure at more acidic domains, supporting stronger gel rigidity and water-holding capacity.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 3
·1740
·
IF 4.9
Yonglin Ruan,Xuehui Li,Gang Zhai,Qiyong Lou,Xia Jin,Jiangyan He,Zhan Yin
The mechanism of fish gonadal sex differentiation is complex and regulated by multiple factors. It has been widely known that proper steroidogenesis in Leydig cells and sex-related genes in Sertoli cells play important roles in gonadal sex differentiation. In teleosts, the precise interaction of these signals during the sexual fate determination remains elusive, especially their effect on the bi-potential gonad during the critical stage of sexual fate determination. Recently, all-testis phenotypes have been observed in thecyp17a1-deficient zebrafish and common carp, as well as incyp19a1a-deficient zebrafish. By matingcyp17a1-deficient fish with transgenic zebrafishTg(piwil1:EGFP-nanos3UTR), germ cells in the gonads were labelled with enhanced green fluorescent protein (EGFP). We classified thecyp17a1-deficient zebrafish and their control siblings into primordial germ cell (PGC)-rich and -less groups according to the fluorescence area of the EGFP labelling. Intriguingly, the EGFP-labelled bi-potential gonads incyp17a1+/+ fish from the PGC-rich group were significantly larger than those of thecyp17a1−/− fish at 23 days post-fertilization (dpf). Based on the transcriptome analysis, we observed that thecyp17a1-deficient fish of the PGC-rich group displayed a significantly upregulated expression ofamhandgsdfcompared to that of control fish. Likewise, the upregulated expressions ofamhandgsdfwere observed incyp19a1a-deficient fish as examined at 23 dpf. This upregulation ofamhandgsdfcould be repressed by treatment with an exogenous supplement of estradiol. Moreover, tamoxifen, an effective antagonist of both estrogen receptor α and β (ERα and Erβ), upregulates the expression ofamhandgsdfin wild-type (WT) fish. Using thecyp17a1- andcyp19a1a-deficient zebrafish, we provide evidence to show that the upregulated expression ofamhandgsdfdue to the compromised estrogen signaling probably determines their sexual fate towards testis differentiation. Collectively, our data suggest that estrogen signaling inhibits the expression ofamhandgsdfduring the critical time of sexual fate determination, which may broaden the scope of sex steroid hormones in regulating gonadal sex differentiation in fish.
研究领域:
发育生物学
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内分泌学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Dechan Lu,Bohan Zhang,Zhikun Shangguan,Yudong Lu,Jingbo Chen,Zufang Huang
Exosomes, extracellular vesicles released by cells, hold potential as diagnostic markers for the early detection of lung cancer. Despite their clinical promise, current technologies lack rapid and effective means to discriminate between exosomes derived from adenocarcinoma in situ (AIS) and early-stage invasive adenocarcinoma (IAC). This challenge arises from the intrinsic structural heterogeneity of exosomes, necessitating the development of advanced methodologies for precise differentiation. Here, we demonstrate a novel approach for plasma exosome detection utilizing multi-receptor surface-enhanced Raman spectroscopy (SERS) technology to differentiate between AIS and early-stage IAC. To accomplish this, we synthesized a stable and uniform two-dimensional SERS substrate (BC/Au NPs film) by fabricating gold nanoparticles onto bacterial cellulose. We then enhanced its capabilities by introducing multi-receptor SERS functionality via modifying the substrate with both low-specificity and physicochemical-selective molecules. Furthermore, by strategically combining all capturer-exosome SERS spectra, comprehensive “combined-SERS spectra” are reconstructed to enhance spectral variations of the exosome. Combining these features with partial least squares regression-discriminant analysis (PLS-DA) modeling significantly improved discriminatory accuracy, achieving 90% sensitivity and 95% specificity in distinguishing AIS from early-stage IAC. Our developed SERS sensor provides an effective method for early detection of lung cancer, thereby paving a new way for innovative advancements in diagnosing lung cancer.
研究领域:
肿瘤学
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医学诊断
·
纳米技术
·
生物信息学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR AND CELLULAR ENDOCRINOLOGY
·
2025
·
IF 3.6
Dan-Dan Jin,Yu-Ying He,Feng Ran,Hong-Yuan Yang,Gui-Jun Yan,Zeng-Ming Yang
Decidualization of endometrial stromal cells is important for pregnancy in rodents and humans. Dysfunction of decidualization can lead to implantation failure and recurrent miscarriage. Although the amount of PGF2α in uterine fluid increases during embryo implantation, what stimulates the production of PGF2α in the uterus and whether PGF2α is involved in embryo implantation and decidualization are still unknown. In our study, human chorionic gonadotropin (hCG), an embryo-derived protein, stimulates human endometrial epithelial cells to increase the levels of PGF2α synthesis-related enzymes (cPLA2, COX-1, COX-2, and AKR1C3) and to secrete PGF2α. Epithelial PGF2α induces EREG and HB-EGF secretion by activating ADAM17. The level of IGFBP1, a marker of human in vitro decidualization, is significantly increased by EREG or HB-EGF. PGF2α is also able to increase the IGFBP1 level in humans during in vitro decidualization. Our results indicate that hCG stimulates uterine epithelial PGF2α production to induce in vitro decidualization through EREG and HB-EGF.
研究领域:
生殖生物学
·
妇科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Gang-gang Zhang,Xiao-cui Lai,Neng-shui Ding,Qi-rong Xiong,Shuai Hou,Hong-wei Duan,Wei-hua Lai
Flower-like gold nanoparticle (AuNF) is widely used as a probe. However, the performance of conventional AuNF is not satisfactory because of complex synthesis, insufficient uniformity, and ordinary optical performance. In this paper, a novel chrysanthemum-like Au@polydopamine (AuNC@PDA) prepared using one-pot method was compared with conventional AuNF@polydopamine (AuNF@PDA) prepared using three-step method. AuNC@PDA had more branch structures with more uniform petaloid shape and narrower particle size distribution compared with AuNF@PDA. The grayscale of AuNC@PDA both in aqueous solution and on nitrocellulose membrane was approximately threefold higher than that of AuNF@PDA. Because of PDA coating, the stability and coupling efficiency of AuNC@PDA were much higher than that of conventional nanoprobes − AuNF and AuNS (gold nanosphere). These results indicated that AuNC@PDA had superior morphological and optical performance. The AuNC@PDA was first utilized as a probe in immunochromatographic assay (ICA), and compared with conventional AuNF@PDA. The limits of detection (LOD) of ICA based on AuNC@PDA, AuNS, and AuNF@PDA were 1.59, 9.58, and 5.13 mIU/mL, respectively, when human chorionic gonadotropin was detected as the target. The LOD of ICA based on AuNC@PDA had 6.0-fold and 3.3-fold improvement compared with that based on AuNS and AuNF@PDA, respectively. Our results could be used as a basis for developing novel probes in practical ICA applications.
研究领域:
生物医学工程
·
纳米技术
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）