MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Shuanglin Deng,Fang Wang,Meng Xiong,Bangxing Han,Hui Yan
The rapid, accurate, and on-site quantitative evaluation of the 3 components (total polysaccharide, total flavonoid, and total saponin) of Polygonatum cyrtonema Hua (PCH) is of great significance to ensure the value of its medicine and food, for this purpose, a handheld near-infrared (NIR) spectrometer was used. The raw NIR spectra were preprocessed by seven methods and their combinations, then three kinds of wavelength selection algorithms, the competitive adaptive reweighted sampling (CARS), random frog (RF) sampling, and Monte Carlo uninformative variable elimination (MCUVE) were applied, and the characteristic wavelengths for total polysaccharide, total saponin, and total flavonoid in PCH were obtained, respectively. Subsequently, the partial least squares (PLS) regression models were developed. The results showed that the combination of first derivative and standard normal variate was the optimal pretreatment method, and CARS had the highest performance of the wavelength selection for total polysaccharide and total flavonoid, while RF was the highest for total saponin. With these selected wavelengths, 3 PLS prediction models were built, and their performances were validated with unknown samples, the results showed that the determination coefficient of the prediction set (R 2 P ) of total polysaccharide, total saponin, and total flavonoid were 0.980, 0.948, and 0.942, respectively, and the corresponding root mean square error of prediction set (RMSEP) were 0.162, 0.142, and 0.052. Furthermore, the residual prediction deviations (RPDs) of the three components were all larger than 3, demonstrating the prediction performance of the models was high. Therefore, the three active functional components in PCH could be rapidly determined by a handheld NIR spectrometer, which would be beneficial for ensuring the quality of PCH.
研究领域:
中药分析
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光谱学
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产品关联
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
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2025
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IF 2.5
Shulei Liu,Fanfan Jia,Xiuchao Xie,Sirui Kang,Mingke Feng,Xiaofei Xiong,Beiei Lin,Xinxin Li,Lubna Khan,Xiaoying Zhang,Chen Chen
Edible mushrooms exhibit a range of pharmacological activities, including anticancer, antimicrobial, antioxidant, anti-obesity, and hepatoprotective effects, thereby holding significant potential for applications in the development of functional foods. This study aimed to evaluate the bioactivities of methanolic extracts from various edible mushrooms (MEMs) found in the Qinling Mountains, China, with the following species used as materials: Suillus granulatus (L.) Roussel ( S. granulatus (L.) Roussel), Ramaria botrytoides (Peck) Corner ( R. botrytoides (Peck) Corner), Flammulina velutipes (Curtis) Singer ( F. velutipes (Curtis) Singer), Agrocybe cylindracea (DC.) Maire ( A. cylindracea (DC.) Maire), Lentinula edodes (Berk.) Sing. ( L. edodes (Berk.) Sing.), Hericium erinaceus (Bull.) Pers. ( H. erinaceus (Bull.) Pers.), White Hypsizygus marmoreus (White H. marmoreus ), Auricularia auricular (L.ex Hook.) Undew ( A. auricular (L.ex Hook.) Undew), Hypsizygus marmoreus (white cultivar) ( H. marmoreus (white cultivar)), Pleurotus eryngii (DC. Fr) Qu. ( P. eryngii (DC. Fr) Qu.), Cordyceps militaris (L.) Link ( C. militaris (L.) Link), Lentinula edodes (Berk.) Pegler ( L. edodes (Berk.) Pegler), Hypsizygus marmoreus (brown cultivar) ( H. marmoreus (brown cultivar)), and Morchella esculenta (L.) Pers. ( M. esculenta (L.) Pers.). We examined the total polyphenol, total flavonoid, and total polysaccharide content of MEMs. And quantified the 12 monomeric phenolic substances contained in the MEMs by high-performance liquid chromatography. The antioxidant activity in vitro was studied by determining the DPPH• scavenging capacity, ABTS + • scavenging capacity, and Fe 2+ reduction capacity, whereas the inhibition rates of α-amylase and pancreatic lipase were used to evaluate the enzyme-inhibiting capacity of each edible mushroom. Significant differences were found in the chemical composition and in vitro activity of the extracts from different sources. Our results showed that R. botrytoides (Peck) Corner had the highest total polyphenols and flavonoid content, which were 28.57 ± 0.85 and 32.33 ± 0.37 mg/g, respectively, among the edible mushrooms. Among the 14 edible mushrooms, Pleurotus eryngii (DC. Fr) Qu had the highest total polysaccharide content of 503.73 ± 0.24 mg/g. The antioxidant activity and pancreatic lipase inhibition of R. botrytoides (Peck) Corner methanol extract were superior to other edible mushrooms, while A. auricular (L.ex Hook.) Undew showed the highest inhibition of α-amylase activity. These results reveal that the active substances in R. botrytoides (Peck) Corner are abundant and have good antioxidant and enzyme inhibitory capacity, which can provide theoretical support for the subsequent development and utilization of R. botrytoides (Peck) Corner. These findings help to inform and guide the exploration and development of the species of edible mushrooms, which can serve as a theoretical basis for the advancement of natural functional foods possessing antioxidant, hypoglycemic, and hypolipidemic effects. Graphical Edible mushrooms are nutritious, low in calories, and extremely high in biologically active substances, making them an important source of nutraceuticals as well as biologically active metabolites for use as drugs.
研究领域:
药理学
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食品科学
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生物化学
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菌物学
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营养学
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天然产物研究
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2020
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IF 2.87
Bianling Jiang,Fuyun Geng,Ran Chang,Mengting Ruan,Ying Bian,Lisheng Xu,Fan Feng,Qiong Chen
The present study was executed to analyze the functional phytochemicals of hulless barley grass grown under different intensities of ultraviolet stress. The wheat seedlings were imposed to 0.5, 1.0, 1.5, 2.0, and 2.5 h ultraviolet radiation and harvested in different times at vegetative stage. Specifically, the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, and chlorophyll were determined and antioxidants capacity was evaluated by OH• and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging ability. A mathematical model (Technique for Order Preference by Similarity to Ideal Solution, TOPSIS) was also employed for the comprehensive evaluation of functional components of hulless barley grass at different growth stages. The results showed that the UV stress could efficiently improve/preserve the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, chlorophyll a, chlorophyll b, and total chlorophyll, as well as the OH• and ABTS scavenging capacity. TOPSIS evaluation revealed that the highest phytochemical contents were yield on the 15th day under 1.0 h ultraviolet treatment.
研究领域:
植物生理学
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植物化学
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农学
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环境胁迫生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）