NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 47
·22581-22592
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IF 3.925
Lei Wang,Shiping Cao,Guoqing Guo,Yang Hu,Jie Li,Xianying Fang,Linguo Zhao
Deep eutectic solvents (DES) have received extensive attention in the extraction of natural products in the last few years. They are considered a substitute for conventional organic solvents because of their environmental advantages. To efficiently extract bioactive polyphenols and triterpenoids from the medicinal plant Celtis sinensis, fifteen DESs were prepared. The resulting DES1 (choline chloride-levulinic acid) showed the maximum extraction yield of total polyphenols, while DES9 (choline chloride-1,2-propylene glycol) had the maximum yield of total triterpenes. Further, the extraction conditions of DES1 and DES9 were methodically optimized. After optimizing the extraction conditions of DES1 and DES9, the extraction yield of total polyphenols was enhanced from 82.05 mg g−1 to 101.38 mg g−1 and that of total triterpenes from 57.87 mg g−1 to 72.72 mg g−1, both significantly superior to those of traditional water- and methanol-based extraction approaches. We designed a new DES by mixing DES1 and DES9 in the ratio of 7.25 : 2.75 to perform extraction of both polyphenols and triterpenes with high efficiency. The new DES solvent simultaneously increased the extraction yields of polyphenols and triterpenes to 118.46 mg g−1 and 88.51 mg g−1, respectively, after response surface methodology. More importantly, the extract obtained by the mixed solvent of DES1 and DES9 displayed significant antioxidant activity superior to those of extracts individually produced by DES1 and DES9. Our results indicate that a DES-based approach has promising potential in extracting bioactive substances from medicinal arbor species with high efficiency and eco-friendliness.
研究领域:
植物化学
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天然产物提取
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药用植物学
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DOI
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2020
·Vol. 31
·期 3
·366-374
·
IF 2.772
Jing-Wen Hao,Yue Chen,Nai-Dong Chen
In this study, the potentiality of applying attenuated total reflectance near-infrared (ATR-NIR) and attenuated total reflectance mid-infrared (ATR-MIR) techniques combined with a partial least squares (PLS) regression technology to quantify the total polyphenols (TPs) in Dendrobium huoshanense (DHS) was investigated and compared. The real TP contents in the DHS samples were analysed using methods of reference. The capability of the two IR spectroscopic techniques to quantify the TPs in DHS was assessed by the root-mean-square error of calibration (RMSEC) and determination coefficients ( R 2 ). The results showed that both NIR and MIR might be used as a fast and simple tool to replace traditional chemical assays for the determination of the TP contents in DHS, and the best NIR model showed slightly better prediction performance [root-mean-square error of prediction (RMSEP): 0.307, R 2 : 0.9122, ratio performance deviation (RPD): 4.43] than the best MIR model (RMSEP: 0.440, R 2 : 0.9069, RPD: 3.09). Results from this study indicated that both the NIR and MIR models could be used to quantify the TP in DHS, and ATR-NIR appeared to be the more predominant and more robust technique for the quantification of the TP in DHS.
研究领域:
植物化学
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分析化学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2022
·
IF 6.76
Yuan Gao,Yutong Shi,Na Miao,Wenxin Xing,Cholil Yun,Shengfang Wang,Wenjie Wang,Huimei Wang
In this study, a green and efficient ultrasound-assisted enzymatic extraction (UAEE) method was established for total polyphenols (TPP) extraction from E. nigrum aerial parts. Fractional Factorial design (FFD) was initially implemented for the selection of significant parameters affecting TPP yield and then Box-Behnken design-response surface methodology (BBD-RSM) was employed to optimize the parameters. The optimum extraction conditions for UAEE were as follows: incubation temperature of 38.39 ℃, incubation time of 3.39 h, tannase of 386.53 U/g of sample (U/g), and cellulase of 224.42 U/g. Under the optimum conditions, the TPP yield was 52.17 ± 0.39 mg/g, which was 1.62 and 1.73 times those of the methods of ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE), respectively. The crude extract was purified by HPD-600 microporous resin, and the purity was improved from 8.11% to 22.56%. In addition, the results of antioxidant and antibacterial activity assays showed that the purified extract has stronger bioactivities in contrast with the crude extract. This study indicated that UAEE was an effective technique for the extraction of TPP from the aerial parts of E. nigrum , and TPP extracted by this extraction method could be applied in pharmaceuticals, cosmetics, and food industries.
研究领域:
植物化学
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天然产物化学
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食品科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Xiao-Qian Fu,Na Ma,Wei-Peng Sun,Yan-Yan Dang
In this study, a novel method of simultaneous extraction and enrichment of total polyphenol (TP) and lutein from marigold flower was developed by microwave and enzyme co-assisted aqueous two-phase extraction (ATPE) (MEAATPE). In this study, ethanol/ammonium sulfate was chosen to construct aqueous two-phase system (ATPS) due to its fine partition and recycling behaviors. The optimal MEAATPE condition was obtained, that is, 28% (w/w), ethanol/20% (w/w) ammonium sulfate, 0.45 U/g enzyme concentration, enzymolysis time of 150 min, enzymolysis temperature of 45 °C, microwave time of 120 s, and microwave power of 270 W, by investigating the effects of ATPS composition and other factors on the yield of target products. Under this optimal condition, the yields of TP and lutein reached 84.61 and 7.32 mg/g with the corresponding recovery rates of 95.35% and 99.85%, respectively. Compared with enzyme-assisted ATPE, microwave-assisted ATPE, ATPE, and Soxhlet extraction (SE), the extraction yield of TP by MEAATPE was the highest, which is 71.14% higher than that of SE. Scanning electron microscopy analysis further showed an optative pretreatment effect of marigold flower powders. Thus, MEAATPE was an efficient, rapid, and environmental-friendly method used for the extraction of natural products.
研究领域:
植物化学
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食品科学
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天然产物提取
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 18
·4288
·
IF 4.2
Mingyu Yang,Yitang Xu,Qihua Yu,Mengyu Li,Liyong Yang,Ye Yang
Xanthine oxidase (XOD) is a key enzyme that promotes the oxidation of xanthine/hypoxanthine to form uric acid, and the accumulation of uric acid leads to hyperuricaemia. The prevalence of gout caused by hyperuricaemia is increasing year by year. TAOZHI (TZ) can be used for the treatment of rheumatic arthralgia due to qi stagnation and blood stasis and contains a large number of polyphenolic components. The aim of this study was to investigate the relationship between chromatograms and XOD inhibition of 21 batches of TZ total polyphenol extract samples. Chemometric methods such as grey correlation analysis, bivariate correlation analysis, and partial least squares regression were used to identify the active ingredient groups in the total polyphenol extracts of TZ, which were validated using molecular docking techniques. The total polyphenol content contained in the 21 batches did not differ significantly, and all batches showed inhibitory effects on XOD. Spectroeffect correlation analysis showed that the inhibitory effect of TZ on XOD activity was the result of the synergistic effect of multiple components, and the active component groups screened to inhibit XOD were F2 (4-O-Caffeoylquinic acid), F4, and F10 (naringenin). The molecular docking results showed that the binding energies of all nine dockings were lower than −7.5 kcal/mol, and the binding modes included hydrogen bonding, hydrophobic forces, salt bridges, and π-staking, and the small molecules might exert their pharmacological effects by binding to XOD through the residue sites of the amino acids, such as threonine, arginine, and leucine. This study provides some theoretical basis for the development and utilisation of TZ total polyphenols.
研究领域:
药理学
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生物化学
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计算生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2019
·Vol. 96
·期 11
·1275-1289
·
IF 1.72
Weifang Wang,Sai Han,Zhen Jiao,Jiangrui Cheng,Junying Song
In this study, Camellia oil is co-extracted from Camellia oleifera seeds and green tea scraps by supercritical carbon dioxide (SC-CO 2 ), which is optimized on the extraction yield, ABTS-scavenging activity, and total polyphenols content (TPC) of oil by single-factor experiments combined with response surface methodology (RSM). The extraction temperature, pressure, dynamic time, carbon dioxide (CO 2 ) flow rate, and seed mass ratio were investigated with single-factor experiments. The results indicated the optimum CO 2 flow rate and dynamic extraction time were 15 L hour −1 and 60 min (i.e., 2.382 kg CO 2 /100 g sample). Furthermore, the complicated effects of extraction temperature (40–50 °C), pressure (20–30 MPa), and seed mass ratio (0.25–0.75) were optimized by RSM based on the Box–Behnken design (BBD). The models with high R -squared values were obtained and used to predict the optimum operating conditions of the process. Under the optimum operating conditions (i.e., temperature of 46 °C, pressure of 30 MPa, and seed mass ratio of 0.35), the extraction yield, ABTS-scavenging activity, and TPC of oil were 14.43 ± 0.17 g/100 g sample, 73.70 ± 0.34%, and 2.18 ± 0.05 mg GAE/g oil, which were in good agreement with the predicted values. In addition, the experiments indicated that the Camellia oil obtained was rich in polyphenols, resulting in better oxidation stability and antioxidant activity than the original oil.
研究领域:
食品科学
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化学工程
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植物化学
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产品关联
DOI
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 6
·1018-1024
·
IF 2.613
Yunchun Liu,Liyun Hua,Wanru Zhu,Chen Liu,Hongrui You,Hongqi Chen
UiO-66-NH 2 nanocomposite was post-modified with 4-mercaptophenylboronic acid (MPBA) by the method of in situ hybridization reaction. The hybrid boronate affinity material UiO-NH 2 @P (TEPIC-co-MPBA) was characterized by scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy. The material was applied as fluorescent probe for the detection of cis -diol containing compounds based on the boronate affinity mechanism, and exhibited high specific selectively. The proposed method exhibited good linearity for the detection of catechol in the range of 0.50 to 8.00 μg ml −1 . The detection limit was 0.13 μg ml −1 . The tactic was successfully applied to analyze the total polyphenols in tea beverages for catechol, and relative recovery was in 98.86–106.00%. Therefore, this work provided a promising strategy for the recognition of cis -diol containing compounds.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 8
·4276
·
IF 4.9
Chenyang Li,Xinxin Qi,Lei Xu,Yuan Sun,Yan Chen,Yuhan Yao,Jun Zhao
This study aimed to investigate the preventive effects of the total polyphenols fromNymphaea candida(NCTP) on LPS-induced septic acute lung injury (ALI) in mice and its mechanisms. NCTP could significantly ameliorate LPS-induced lung tissue pathological injury in mice as well as lung wet/dry ratio and MPO activities (p< 0.05). NCTP could significantly decrease the blood leukocyte, neutrophil, monocyte, basophil, and eosinophil amounts and LPS contents in ALI mice compared with the model group (p< 0.05), improving lymphocyte amounts (p< 0.05). Moreover, compared with the model group, NCTP could decrease lung tissue TNF-α, IL-6, and IL-1β levels (p< 0.05) and downregulate the protein expression of TLR4, MyD88, TRAF6, IKKβ, IκB-α, p-IκB-α, NF-κB p65, p-NF-κB p65, NLRP3, ASC, and Caspase1 in lung tissues (p< 0.05). Furthermore, NCTP could inhibit ileum histopathological injuries, restoring the ileum tight junctions by increasing the expression of ZO-1 and occludin. Simultaneously, NCTP could reverse the gut microbiota disorder, restore the diversity of gut microbiota, increase the relative abundance of Clostridiales and Lachnospiraceae, and enhance the content of SCFAs (acetic acid, propionic acid, and butyric acid) in feces. These results suggested that NCTP has preventive effects on septic ALI, and its mechanism is related to the regulation of gut microbiota, SCFA metabolism, and the TLR-4/NF-κB and NLRP3 pathways.
研究领域:
药理学
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免疫学
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微生物学
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病理学
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分子生物学
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DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Shulei Liu,Fanfan Jia,Xiuchao Xie,Sirui Kang,Mingke Feng,Xiaofei Xiong,Beiei Lin,Xinxin Li,Lubna Khan,Xiaoying Zhang,Chen Chen
Edible mushrooms exhibit a range of pharmacological activities, including anticancer, antimicrobial, antioxidant, anti-obesity, and hepatoprotective effects, thereby holding significant potential for applications in the development of functional foods. This study aimed to evaluate the bioactivities of methanolic extracts from various edible mushrooms (MEMs) found in the Qinling Mountains, China, with the following species used as materials: Suillus granulatus (L.) Roussel ( S. granulatus (L.) Roussel), Ramaria botrytoides (Peck) Corner ( R. botrytoides (Peck) Corner), Flammulina velutipes (Curtis) Singer ( F. velutipes (Curtis) Singer), Agrocybe cylindracea (DC.) Maire ( A. cylindracea (DC.) Maire), Lentinula edodes (Berk.) Sing. ( L. edodes (Berk.) Sing.), Hericium erinaceus (Bull.) Pers. ( H. erinaceus (Bull.) Pers.), White Hypsizygus marmoreus (White H. marmoreus ), Auricularia auricular (L.ex Hook.) Undew ( A. auricular (L.ex Hook.) Undew), Hypsizygus marmoreus (white cultivar) ( H. marmoreus (white cultivar)), Pleurotus eryngii (DC. Fr) Qu. ( P. eryngii (DC. Fr) Qu.), Cordyceps militaris (L.) Link ( C. militaris (L.) Link), Lentinula edodes (Berk.) Pegler ( L. edodes (Berk.) Pegler), Hypsizygus marmoreus (brown cultivar) ( H. marmoreus (brown cultivar)), and Morchella esculenta (L.) Pers. ( M. esculenta (L.) Pers.). We examined the total polyphenol, total flavonoid, and total polysaccharide content of MEMs. And quantified the 12 monomeric phenolic substances contained in the MEMs by high-performance liquid chromatography. The antioxidant activity in vitro was studied by determining the DPPH• scavenging capacity, ABTS + • scavenging capacity, and Fe 2+ reduction capacity, whereas the inhibition rates of α-amylase and pancreatic lipase were used to evaluate the enzyme-inhibiting capacity of each edible mushroom. Significant differences were found in the chemical composition and in vitro activity of the extracts from different sources. Our results showed that R. botrytoides (Peck) Corner had the highest total polyphenols and flavonoid content, which were 28.57 ± 0.85 and 32.33 ± 0.37 mg/g, respectively, among the edible mushrooms. Among the 14 edible mushrooms, Pleurotus eryngii (DC. Fr) Qu had the highest total polysaccharide content of 503.73 ± 0.24 mg/g. The antioxidant activity and pancreatic lipase inhibition of R. botrytoides (Peck) Corner methanol extract were superior to other edible mushrooms, while A. auricular (L.ex Hook.) Undew showed the highest inhibition of α-amylase activity. These results reveal that the active substances in R. botrytoides (Peck) Corner are abundant and have good antioxidant and enzyme inhibitory capacity, which can provide theoretical support for the subsequent development and utilization of R. botrytoides (Peck) Corner. These findings help to inform and guide the exploration and development of the species of edible mushrooms, which can serve as a theoretical basis for the advancement of natural functional foods possessing antioxidant, hypoglycemic, and hypolipidemic effects. Graphical Edible mushrooms are nutritious, low in calories, and extremely high in biologically active substances, making them an important source of nutraceuticals as well as biologically active metabolites for use as drugs.
研究领域:
药理学
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食品科学
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生物化学
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菌物学
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营养学
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天然产物研究
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产品关联
DOI
PubMed
高影响因子
Chemical and Biological Technologies in Agriculture
·
2023
·Vol. 10
·期 1
·1-18
·
IF 6.6
Biswas Ashok,Dey Susmita,Xiao Aiping,Deng Yong,Birhanie Ziggiju Mesenbet,Roy Rana,Akhter Delara,Liu Liangliang,Li Defang
Background Besides fibre production, Corchorus olitorius is considered a nutritional and medicinal plant in traditional medicine. Its leaves possess different antioxidant compounds and display various biological properties. This study optimized the ultrasound-assisted extraction (UAE) parameters like temperature, time, solvent concentration, and liquid–solid ratio for total flavonoids (TFC) and total polyphenols content (TPC) from leaves employing response surface methodology (RSM). Results Findings demonstrated that under the optimized conditions, the highest extraction yield of total flavonoids (7.17 mg QE/g DW) and total polyphenol content (13.92 mg GAE/g DW) were recorded with the ethanol concentration 70.92%, temperature 68.06 °C, liquid–solid ratio 48.80 mL/mg, and ultrasound irradiation time 37.20 min. The optimum value of TFC (6.96 mg QE/g DW) and TPC (13.38 mg GAE/g DW) from the experiment of verification of optimized conditions was close to the predicted value and significantly superior to the conventional heat reflux extraction (HRE). LC–MS and HPLC analysis of the optimized extract from UAE demonstrated the existence of six major phenolic compounds, including chlorogenic acid, isoquercetin, hyperoside, adhyperforin, 1,3-di- O -caffeoylquinic acid, and 3,4-di- O -caffeoylquinic acid. Furthermore, the antioxidant test of the UAE leaves extract revealed an excellent 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS + •) and hydroxyl radical scavenging with IC 50 values of 226.29, 199.53 and 402.02 µg/mL, respectively, compared to HRE with 336.31 µg/mL, 253.86 µg/mL, 520.08 µg/mL. Conclusions The developed optimization method could contribute to the good recovery of natural antioxidants from C. olitorius in the pharmaceuticals and food industries. Graphical Abstract
研究领域:
药学
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食品科学
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分析化学
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植物学
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产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Junxia Gao,Turghun Muhammad,Gaowei Guo,Jia Zhao,Yan Zhang
Plant polyphenols, especially their flavonoid subclass are important bioactive compounds. Simultaneous determination of total flavonoids (TFs) and total polyphenols (TPs) is crucial for studying the distribution characteristics of polyphenols. Traditional methods for determining TFs and TPs require complicated operational procedures, and reported flow injection analysis (FIA) methods have also been limited due to inefficiencies caused by carrier changing and system resetting. Simultaneous determination of TFs and TPs is difficult to achieve with the existing methods. Therefore, in this study, a merging-zone FIA technique was developed for the simultaneous determination of TFs and TPs based on the study of the AlCl3 and Folin-Ciocalteu colorimetric reaction mechanisms. The FIA parameters were optimized by univariate experimental design, and standard curves for TFs and TPs were established in the concentration ranges of 10-800 mg/L and 15-400 mg/L, respectively. The limit of detection of the proposed FIA method for TFs and TPs was 3.114 mg/L and 4.166 mg/L, and the limit of quantitation was 10.38 mg/L and 13.89 mg/L. The relative standard deviations were below 2.5% (n=15). The recoveries of spiked sample ranged from 93.89% to 103.8%, and the sample throughput was approximately 67 h-1. The proposed method was successfully applied to the simultaneous determination of TFs and TPs in nine plants, and the analytical results were in good agreement with those of traditional methods. This study provides a rapid, automated and reliable method for simultaneous quantitative analysis, showing high application potential for simultaneous evaluation of these two bioactive compounds in various plants.
研究领域:
分析化学
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植物生物化学
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植物化学
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流动分析
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分光光度分析
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Bianling Jiang,Fuyun Geng,Ran Chang,Mengting Ruan,Ying Bian,Lisheng Xu,Fan Feng,Qiong Chen
The present study was executed to analyze the functional phytochemicals of hulless barley grass grown under different intensities of ultraviolet stress. The wheat seedlings were imposed to 0.5, 1.0, 1.5, 2.0, and 2.5 h ultraviolet radiation and harvested in different times at vegetative stage. Specifically, the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, and chlorophyll were determined and antioxidants capacity was evaluated by OH• and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging ability. A mathematical model (Technique for Order Preference by Similarity to Ideal Solution, TOPSIS) was also employed for the comprehensive evaluation of functional components of hulless barley grass at different growth stages. The results showed that the UV stress could efficiently improve/preserve the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, chlorophyll a, chlorophyll b, and total chlorophyll, as well as the OH• and ABTS scavenging capacity. TOPSIS evaluation revealed that the highest phytochemical contents were yield on the 15th day under 1.0 h ultraviolet treatment.
研究领域:
植物生理学
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植物化学
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农学
·
环境胁迫生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）