JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Jingtao Cui,Jun Cao,Jiachen Ge,Xingchen Qu,Pengyan Li,Chuan Li
Cobia ( Rachycentron canadum ) is a significant fish species for mariculture with high nutritional value . This research aims to comprehensively investigate the lipid profiles of cobia tissues, including fatty acid profiles, characteristics of phospholipids (PL) and triacylglycerols (TAG), and lipid molecular species. The results showed that cobia contained high levels of total lipids (2.89 −32.09 % of wet weight), docosahexaenoic acid (DHA, 10.83 −14.69 % of total fatty acids) and eicosapentaenoic acid (EPA, 3.54 −9.68 %). Compared with TAG, higher levels of polyunsaturated fatty acids , DHA/EPA in particular, were concentrated in PL. Interestingly, DHA was predominantly distributed in the sn-1 position of PL and the sn-2 position of TAG, whereas EPA was predominantly distributed in the external positions of PL and TAG. Additionally, at least 693 molecular species from 23 subclasses were identified by ultrahigh-performance liquid chromatography-quadrupole exactive-mass spectrometry (UPLC-Q-Exactive-MS). Glycerophospholipids (63.19 −66.61 %) and glycerolipids (19.17 −22.21%) were the major lipid classes in cobia tissues, and the majority of them contained DHA and EPA. Hence, this comprehensive analysis will help to evaluate the lipid nutritional values and facilitate exploitation in cobia consumption and processing.
研究领域:
水产养殖学
·
营养学
·
脂质组学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Rui Mao,Wei Li,Penghao Jia,Hui Ding,Tekleab Teka,Lin Zhang,Zhifei Fu,Xuebin Fu,Sunjay Kaushal,Zhiying Dou,Lifeng Han
Unsaturated fatty acids (UFAs) are fatty acids with one or more carbon-carbon double bonds (C=C). They are building blocks of structurally complex lipids. According to the position of C=C bond in the UFAs, there are multiple isomers with different physiological activities. Therefore, an accurate understanding of UFAs is essential. Combining Paternò-Büchi (PB) reaction with tandem mass spectrometry (MS/MS) analysis has been successfully used to identify unsaturated lipids in bio-samples. However, there are still some challenges, such as low reactive rate and low MS response of PB products. In this study, 3-pyridinecarboxaldehyde (3-PYA) was selected as an efficient PB reagent for the first time. According to the nitrogen rule, introduction of nitrogen did not only make the target MS and MS/MS ions to be easily identified but also increase the ionization efficiency significantly. The developed method in this study demonstrated greater efficiency and sensitivity for the identification of UFAs in total lipid extracts from bovine liver compared with other commonly used PB reagents.
研究领域:
分析化学
·
脂质组学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 19
·19450-19460
·
IF 2.76
Zhou Qiongzhi,Li Feng,Ge Fei,Liu Na,Kuang Yangduo
Toxicants are generally harmful to biotechnology in wastewater treatment. However, trace toxicant can induce microbial hormesis, but to date, it is still unknown how this phenomenon affects nutrient removal during municipal wastewater treatment process. Therefore, this study focused on the effects of hormesis induced by cetyltrimethyl ammonium bromide (CTAB), a representative quaternary ammonium cationic surfactant, on nutrient removal by Chlorella vulgaris F1068. Results showed that when the concentration of CTAB was less than 10 ng/L, the cellular components chlorophyll a , proteins, polysaccharides, and total lipids increased by 10.11, 58.17, 38.78, and 11.87 %, respectively, and some enzymes in nutrient metabolism of algal cells, such as glutamine synthetase (GS), acid phosphatase (ACP), H + -ATPase, and esterase, were also enhanced. As a result, the removal efficiencies of ammonia nitrogen (NH 4
+ ) and total phosphorus (TP) increased by 14.66 and 8.51 %, respectively, compared to the control during a 7-day test period. The underlying mechanism was mainly due to an enhanced photosynthetic activity of C. vulgaris F1068 indicated by the increase in chlorophyll fluorescence parameters (the value of Fv / Fm , ΦII, Fv / Fo , and rETR increased by 12.99, 7.56, 25.59, and 8.11 %, respectively) and adenylate energy charge (AEC) (from 0.68 to 0.72). These results suggest that hormesis induced by trace toxicants could enhance the nutrient removal, which would be further considered in the design of municipal wastewater treatment processes.
The schematic mechanism of C. vulgaris F1068 under CTAB induced hormesis. Green arrows ( ) represent the increase and the red arrow ( ) represents the decrease.
研究领域:
环境科学
·
微生物学
·
生物技术
·
废水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）