JOURNAL OF MEDICINAL FOOD
·
2014
·Vol. 17
·期 2
·236-243
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IF 1.699
Qisi Lin,Miao Wang,Jinghua Li,Wanping Shi,Hui Wang,Chunjie Zhao
Recineckea carnea and Tupistra chinensis collected from the Guizhou province (China) were evaluated in this study. Petroleum ether fractions from the two herbs were subjected to gas chromatography–mass spectrometry analysis; 10 species, which were fatty acids or aliphatic esters, were identified. The antimicrobial activities of a variety of extracts were evaluated against four microorganisms. The methanol extract (ME), chloroform fraction, and ethyl acetate fraction from T. chinensis exhibited antimicrobial activities comparable to standard antibiotics, whereas none of the investigated extracts from R. carnea demonstrated any antimicrobial activities. The antioxidant potential was evaluated in vitro using ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical method. The FRAP value of the ME from T. chinensis (4.19±0.088 mmol/g) was found to be significantly higher than the analogous extract from R. carnea (2.39±0.092 mmol/g); the EC50 of the ME from R. carnea (0.32±0.011 mg/mL) was found to be significantly higher than that of T. chinensis (0.30±0.015 mg/mL). Total phenolic content was estimated by the Folin–Ciocalteu's colorimetric method. A positive correlation was found between total phenolic content and antioxidant activities (FRAP value and the reciprocal of EC50). The results suggested that the phenolic compounds contributed significantly to the antioxidant capacity of R. carnea and T. chinensis.
研究领域:
植物化学
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药理学
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微生物学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Richard Osae,Maurice Tibiru Apaliya,Emmanuel Kwaw,Michelle T.R. Chisepo,Otu Phyllis Naa Yarley,Eric Aforo Antiri,Raphael N. Alolga
This work aimed to determine the effects of five drying methods on the quality and essential oil composition of Ghanaian ginger. To this end, slices of fresh ginger rhizomes were dried using the following dryers; relative humidity convective (RHC) dryer, freeze (F) dryer, infrared (IR) dryer, microwave (MC) dryer and pulsed vacuum (PV) dryer. The antioxidant activities, total phenolic content (TPC), sensory characteristics and moisture content of the dried slices were assessed. The effects of the various drying techniques on the chemical composition of the essential oil components were determined using gas chromatography-mass spectrometry (GC–MS). With respect to the sensory attributes of the various dried ginger samples, the RHC-dried samples attained the highest scores for aroma, flavor and general acceptability while the F-dried samples scored the highest marks for appearance (i.e., colour). The RHC-dried samples had the lowest moisture content (5.45 %) followed by the F-dried (6.36 %), IR-dried (8.06 %), MC-dried (9.15 %) and PV-dried (10.07 %) samples. The F-dried samples retained the highest TPC (110.36 mg Gallic acid equivalent (GAE)/g db) and exhibited the highest antioxidant activities as evidenced by the outcomes of 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power capacity (FRAP) tests. The chemical profiles of the essential oil extracted from the various dried ginger samples were distinct and unique. The essential oil obtained from the F-dried samples was observed to have the highest compounds (24). The number of compounds identified in the oil extracted from the other dried samples were : RHC drying, 23; IR drying, 16; MC drying, 15; PV drying, 3. In general, the samples subjected to F and RHC drying gave the best outcomes for all the quality parameters assessed and are therefore recommended for large-scale industrial application.
研究领域:
食品科学
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植物化学
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DOI
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2016
·Vol. 17
·期 5
·391
·
IF 1.303
Yun Xing,Le Cai,Tian-peng Yin,Yang Chen,Jing Yu,Ya-rong Wang,Zhong-tao Ding
The antioxidant activities and total phenolic content of fermented Salvia miltiorrhiza with fungus Geomyces luteus were investigated. The results revealed that G. luteus fermentation could significantly improve the antioxidant activity and total phenolic content of S. miltiorrhiza . The main antioxidant constituents were characterized by spectroscopic analysis as salvianolic acids. High-performance liquid chromatography (HPLC) quantification also showed the enhanced content of salvianolic acid B after fermentation. The present study suggests that G. luteus fermentations are effective in the S. miltiorrhiza salvianolic acids’ enrichment process.
研究领域:
药用植物学
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天然产物化学
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发酵工程
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Guan-Lin Chen,Song-Gen Chen,Yi Xiao,Nan-Lin Fu
In order to find new sources of natural antioxidants, total phenolic content (TPC) and total flavonoid content (TFC) of 30 flowers in their free, esterified and insoluble-bound forms were determined. Rosa rugosa Thunb (pink) showed the highest TPC, and Osmanthus fragrans had the highest TFC. The antioxidant activities of 30 flowers were determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) assays. Rosa rugosa Thunb (purple) and Rosa rugosa Thunb (pink) had the highest DPPH (612.79 and 544.75 μmol Trolox/g DW), FRAP (273.10 and 301.14 μmol Trolox/g DW) and TEAC (1013.71 and 937.19 μmol Trolox/g DW) value. Furthermore, several phenolic compounds were detected using ultra performance liquid chromatography (UPLC) technique, and (+)-catechin, ferulic acid, isoquercitrin, and quercitrin were widely found in these flowers. The results implied that the flowers were important natural sources of bioactive components with higher antioxidant capacities for use in the food and pharmaceutical industries.
研究领域:
植物学
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食品科学
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药理学
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产品关联
DOI
高影响因子
RSC Advances
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2022
·Vol. 12
·期 37
·24242-24251
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IF 4.036
Xiaoxia Duan,Xueke Wang,Jiangwei Chen,Guijiang Liu,Yun Liu
In order to investigate the solubility behavior of lignin in formic acid (FA) solution Phragmites australis biomass was subjected to a sequential two-step formosolv fractionation using 88% FA followed by 70% FA to obtain four specific lignin fractions, designated as IFL-88%, IFSL-70%, IFIL-70% and IFL-EtAc. The structures of the four isolated lignin fractions were successfully characterized by gel permeation chromatography (GPC), Fourier transform infrared (FTIR) spectroscopy, two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy (2D-HSQC NMR), thermogravimetric analysis (TGA), and gas chromatography-mass spectroscopy (GC/MS). Furthermore, the total phenolic content of the four isolated lignin samples was assessed by Folin-Ciocalteu analysis. The data from structural properties revealed that depolymerization of the isolated lignin fractions occurred via β-O-4 cleavage, accompanied by competitive condensation reaction. Interestingly, 70% aqueous FA could separate specific lignin fractions with different antioxidant capacities of ABTS˙+ and DPPH radical scavenging activity. Due to the high total phenolic hydroxyl content (25%) and low molecular weight (Mw = 2760 Da) and polydispersity index (PDI = 1.5), IFL-EtAc lignin showed excellent antioxidant activity at the same concentration of 2.0 mg mL−1 in comparison with three other isolated lignin fractions, and it was even equal to that of commercial antioxidant butylated hydroxytoluene (BHT). These findings are helpful to separate specific lignins with higher value as potential antioxidants by sequential two-step formosolv fractionation in lignin chemistry.
研究领域:
植物化学
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分析化学
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DOI
PubMed
高影响因子
ACS Food Science & Technology
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2025
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IF 2.8
Walaa Kobacy,Ahmed K. Rashwan,Ahmed I. Osman,Ashraf K. Abu-Zaid,Ahmed R. Mazeed,Reda M. Mohamed
This study investigates the physicochemical, antioxidant, and anticancer properties of four Egyptian honey types: clover, fennel, sidr, and multiflower. These honeys exhibited significant variability in color, pH, acidity, sugars, and enzymatic activity. The fennel honey demonstrated the highest content of total phenolic (TPC) of 238.76 mg GAE/100 g, and the total flavonoid (TFC) was 94.06 mg QE/100 g. In addition, the antioxidant activity as measured by DPPH radical scavenging correlated strongly with bioactive compound concentrations, with fennel honey showing the highest inhibition (60.14%). In vitro cytotoxicity assays against Hep-2 human laryngeal cancer cells revealed fennel honey to have the lowest IC50 value (70 μg/mL), followed by multiflower honey (IC50 = 87 μg/mL), confirming a significant relationship between the TPC, TFC, and the anticancer effects. This highlights the role of bioactive compounds in honey as potential natural anticancer agents. Clover honey exhibited the weakest cytotoxicity (IC50 = 130 μg/mL) with the lowest TPC and TFC. These findings underscore the potential of Egyptian honey varieties, particularly fennel and multiflower honeys, as promising natural sources of antioxidants and anticancer agents, suggesting a beneficial therapeutic role in cancer prevention. Further studies are needed to elucidate the detailed mechanisms by which honey compounds exert these anticancer effects.
研究领域:
食品科学
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肿瘤学
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生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Yaping Zheng,Kangmeng Sun,Xinyuan Sun,Yue Li,Peigen Xiao,Chunnian He
Sea buckthorn is rich in active ingredients, widespread, and has both medicinal and nutritional value. The present comparative study of wild and cultivated species remains insufficient, which is not conducive to their quality control. Therefore, this study aimed to compare the differences of 21 sea buckthorn samples in total phenolic content (TPC), total flavonoid content (TFC), phenolic components content, secondary metabolites, and antioxidant capacity and the fatty acid, to investigate the quality differences of different varieties. The TPC, TFC and antioxidant activity of wild varieties were higher than those of the cultivated. Multivariate statistical analysis revealed large differences in phenolic content, with higher levels of gallic acid and isorhamnetin-3- O -neohesperidin in the wild, whereas the cultivated were characterized by narcissin and kaempferol. These findings provided the scientific basis for the improvement of quality evaluation standards for different varieties and offered new insights for the further development of sea buckthorn resources.
研究领域:
植物学
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营养学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2015
·Vol. 39
·期 5
·566-574
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IF 0.741
Wu Zun-Qiu,Yue Gui-Zhou,Zhu Qing-Ping,Jiang You-Jun,Tang Kai-Yu,Chen Hua-Ping,Yang Ze-Shen,Huang Qian-Ming
Oleuropein was extracted from olive leaves by ultrasonic-assisted method and purified by silica gel column chromatography. The sample was identified as oleuropein by UV, infrared, mass spectroscopy and nuclear magnetic resonance spectroscopy analysis. Oleuropein and total phenolic contents over a year were determined by high-performance liquid chromatography and Folin–Ciocalteu methods. The results showed that 13.52% of pure oleuropein was found with a high purity of 96.54% and purification efficiency of 78.49%. Oleuropein and total phenolic contents of different olive varieties were quite different, but both had similar trends over the year. Five methods, including DPPH (2,2-diphenyl-1-picrylhydrazyl), ABST (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), ferric reducing antioxidant power, total reducing power and nitrite-scavenging ability, were used to evaluate the antioxidant activities of oleuropein, and the IC 50 were 34.54 ± 0.14 μg/mL, 18.79 ± 0.82 μg/mL, 75.32 ± 1.83 μg/mL, 13.80 ± 0.68 μg/mL and 1.00 ± 0.08 mg/mL, respectively. Practical Applications This study provides information on the changes in contents including oleuropein and total phenolics in a year to help understand the relationship between content of oleuropein and various influencing factors. Currently, the metabolic pathways of oleuropein are not clear. These results provide information for the study of the metabolism of phenolic compounds in olive and promote research at the molecular level. Oleuropein occupies a large proportion of total polyphenols in olive and can be to be purified by silica gel column chromatography with high purification efficiency for industrial production and laboratory production. In addition, the comprehensive evaluation of activity in vitro shows that oleuropein is more useful than BHT (2,6-di- tert -butyl-4-methylphenol) for the application in food and human health.
研究领域:
植物化学
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食品科学
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分子生物学
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产品关联
DOI
高影响因子
Journal of Functional Foods
·
2016
·
IF 3.973
Yu-Kun Wang,Xin Zhang,Guan-Lin Chen,Jing Yu,Lu-Qi Yang,Yong-Qing Gao
To explore the potential of common beans as natural sources of bioactive compounds, the antioxidant properties and total phenolic content (free, soluble conjugate, insoluble-bound fraction) of 14 beans from China were evaluated systematically. The results showed that beans had diverse antioxidant capacities and the variation was very large. Black bean, flower waist bean, pearl bean and spring bay bean showed the strongest antioxidant activities among the 14 tested samples based on a combinative consideration of the results obtained by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, and trolox equivalent antioxidant capacity (TEAC) assay. Furthermore, several phenolic compounds were detected; gallic acid and protocatechuic acid were widely found in these beans. The results implied that these beans were important natural sources for preventing oxidative stress diseases.
研究领域:
食品科学
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植物化学
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营养学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2014
·Vol. 49
·期 10
·2290-2298
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IF 1.354
Yurong Ma,Huihua Huang
Summary To investigate the relationship between bioactive compounds and antioxidant property, total phenolic content (TPC), total flavonoid content (TFC), individual and total isoflavone content of soymilk and their correlations with oxygen-radical-absorbing capacity (ORAC), ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity values were evaluated and compared. Results showed that TPC, TFC, isoflavones (including concentration and profiles) and antioxidation activity significantly varied among ten tested soybean cultivars. Significant correlations were established between DPPH and TFC ( r = 0.553, P < 0.01), DPPH and TPC ( r = 0.753, P < 0.01), FRAP and TFC ( r = 0.599, P < 0.01) and FRAP and TPC ( r = 0.616, P < 0.01). Positive linear correlations were found between subtotal, total isoflavones and ORAC. Unlike other isoflavone monomers, aglycone isoflavones correlated positively with DPPH and FRAP significantly. DPPH well correlated with FRAP, whereas neither DPPH nor FRAP correlated with ORAC. TPC, TFC, individual and total isoflavone values are potentially useful for soymilk antioxidant activity assessment.
研究领域:
食品科学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2014
·Vol. 240
·期 4
·815-822
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IF 1.387
Tan Li-Hong,Zhang Dan,Yu Bao,Zhao Sheng-Ping,Cao Wei-Guo
The leaf of Magnolia officinalis is abundant in natural resources as a novel potential antioxidant and has great commercial value. In this study, the contents of total phenolics, total flavonoids, magnolol and honokiol in different solvent extracts (acetone, methanol and water) were measured. Meanwhile, the antioxidant activity of extracts from M. officinalis leaves were evaluated using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical scavenging assay, FRAP assays and β-carotene/linoleic acid system. In addition, the correlation between the antioxidant activity and the contents of the active compounds was analyzed. Experimental results indicated that three extracts exhibited antioxidant effects in a concentration-dependent manner, and acetone extracts had the highest antioxidant activity, next methanol and water. There was an intimate relativity between the contents of magnolol and honokiol in different solvent extracts and the antioxidant capability of them. So the magnolol and honokiol of M. officinalis leaves as a representative of the active compounds were extracted and purified by using the macroporous resin column. The purity of depurated sample could reach 82.6 %, which indicates the M. officinalis leaves have the potential to substitute the production of magnolol and honokiol in M. officinalis . The results of this study can provide theoretical and technical references for the reasonable use of the M. officinalis leaves and alleviate the shortage of M. officinalis resources.
研究领域:
植物化学
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药用植物学
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天然产物化学
✓ 已验证
DOI
高影响因子
Biomed Research International
·
2016
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IF 2.134
Lin Zhou,Xiaohui Lin,Arshad Mehmood Abbasi,Bisheng Zheng
Sesame (Sesamum indicum L.) seeds are popular nutritional food but with limited knowledge about their antioxidant and antiproliferative activities of various varieties. Phytochemical profiles and antioxidant and antiproliferative activities of six varieties of sesame (Sesamum indicum L.) seeds were studied. Fenheizhi3 (black) cultivar exhibited the maximum contents of total phenolics and lignans and values of total oxygen radical absorbance capacity (ORAC) and antiproliferative activity (EC50) against HepG2 cells. Bound ORAC values showed strong associations with bound phenolics contents (, ); in bound phenolic extracts, EC50 values showed strong negative associations with phenolic contents (, ) and ORAC values (, ). Moreover, the contents of free phenolics were higher than that of the bound phenolics, and the three black sesame seeds generally depicted higher total phenolics compared to the three white varieties. The antioxidant (ORAC values) and antiproliferation activities of six sesame seeds were both associated with contents of bound phenolics (, ). Interestingly, nonlignan components in bound phenolics contributed to the antioxidant and antiproliferative activities. This study suggested that Fenheizhi3 variety is superior to the other five varieties as antioxidant supplements.
研究领域:
植物化学
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营养学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）