Foods
·
2023
·Vol. 12
·期 18
·3495
·
IF 5.2
Zhiyu Sun,Yongbin Zhou,Wenxu Zhu,You Yin
As a traditional cash crop with ecological and nutritional values, mulberry is gradually expanding its consumption worldwide due to its great regional adaptability and superior health functions. The widespread interest in nutrients has led to a growing need to explore in depth the health benefits of mulberries. Many studies are actively being conducted to investigate the adaptability of the diversity of mulberries in different applications. This study systematically investigated the physicochemical properties and antioxidant activity of four mulberry genotypes cultivated in China’s semi-arid sandy regions to better understand the composition and health-promoting potential of this super crop. Chemical composition identification was identified via HPLC and antioxidant activity was further determined via DPPH and FRAP. The moisture, crude protein, ash, soluble solids, phenolics, anthocyanins, and flavonoids contents of mulberry were comparatively analyzed. The study revealed that the four mulberry genotypes showed significant differences in quality and content of the analyzed characteristics. The greatest antioxidant activity was found in Shensang 1, which had the most soluble solids (17%) and the highest amounts of free sugar (fructose: 5.14% and glucose: 5.46%). Ji’an had the most minerals (K: 2.35 mg/g, Ca: 2.27 mg/g, and Fe: 467.32 mg/kg) and it also contained chlorogenic acid, which has the potential to be turned into a natural hypoglycemic agent. PCA and Pearson correlation analysis indicated that the antioxidant activity was closely related to the chemical contents of total phenols, flavonoids, anthocyanins, and soluble sugars. If the antioxidant activity and nutrient content of the developed plants are considered, Shen Sang 1 is the most favorable variety. This finding can be used to support the widespread cultivation of mulberries to prevent desertification as well as to promote the development of the mulberry industry.Keywords:mulberries fruit characteristics;total phenolics;antioxidant activity
研究领域:
农业
·
营养学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Lei Liu,Ruifen Zhang,Yuanyuan Deng,Yan Zhang,Juan Xiao,Fei Huang,Wei Wen,Mingwei Zhang
In this study, rice bran was successively steamed with α-amylase, fermented with lactic acid bacteria, and hydrolyzed with complex enzymes. The changes in phenolic profiles and antioxidant activities of the corresponding aqueous solutions from three stages were investigated. Compared to the first stage, fermentation and complex enzyme hydrolysis significantly increased the total phenolics, total flavonoids, total FRAP and ORAC values by 59.2%, 56.6%, 73.6% and 45.4%, respectively. Twelve individual phenolics present in free or soluble conjugate forms were also analyzed during the processing. Ferulic acid was released in the highest amount among different phenolics followed by protocatechuic acid. Moreover, a major proportion of phenolics existed as soluble conjugates. The results showed that fermentation and complex enzyme hydrolysis enhanced total phenolics and antioxidant activities of aqueous solution from rice bran pretreated by steaming with α-amylase. This research could provide basis for the processing of rice bran beverage rich in phenolics.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Qiu-Lan Liu,Yang Yi,Shi-Qi Wang,Hong-Xun Wang,Wei Xu,Ting Min,Li-Mei Wang
Lotus root is the most consumed aquatic vegetable in China. Both the actual boiling of lotus root and the simulated boiling of its extract were performed at different conditions to explore the development of non-enzymatic browning and the loss of reducing sugars, free amino acids, total free phenols and ascorbic acid. The kinetic parameters of zero-order and first-order models indicated that both browning degree and nutrient loss had a positive correlation with boiling time, temperature and pH value. Moreover, nutrient contents and boiling conditions were adopted as independent variables to establish the multiple linear regression model of browning degree ( P < 0.001), which confirmed that reducing sugars and total phenols principally contributed to the browning ( P < 0.01), through caramelization and phenol oxidation. With the addition of baking soda (pH 8), 100 °C boiling decreased the DPPH radical scavenging capacity and ferric reducing antioxidant power of lotus root extract by 62.96% and 58.51%, respectively. Furthermore, 120 °C boiling probably induced the formation of browning products, which possessed cytotoxicity against HepG2 and L02 cells. A short boiling time, as well as an acidic condition induced by added vinegar, is recommended for cooking and processing of lotus root in consideration of nutrition and health.
研究领域:
食品科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL FOOD
·
2014
·Vol. 17
·期 2
·236-243
·
IF 1.699
Qisi Lin,Miao Wang,Jinghua Li,Wanping Shi,Hui Wang,Chunjie Zhao
Recineckea carnea and Tupistra chinensis collected from the Guizhou province (China) were evaluated in this study. Petroleum ether fractions from the two herbs were subjected to gas chromatography–mass spectrometry analysis; 10 species, which were fatty acids or aliphatic esters, were identified. The antimicrobial activities of a variety of extracts were evaluated against four microorganisms. The methanol extract (ME), chloroform fraction, and ethyl acetate fraction from T. chinensis exhibited antimicrobial activities comparable to standard antibiotics, whereas none of the investigated extracts from R. carnea demonstrated any antimicrobial activities. The antioxidant potential was evaluated in vitro using ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical method. The FRAP value of the ME from T. chinensis (4.19±0.088 mmol/g) was found to be significantly higher than the analogous extract from R. carnea (2.39±0.092 mmol/g); the EC50 of the ME from R. carnea (0.32±0.011 mg/mL) was found to be significantly higher than that of T. chinensis (0.30±0.015 mg/mL). Total phenolic content was estimated by the Folin–Ciocalteu's colorimetric method. A positive correlation was found between total phenolic content and antioxidant activities (FRAP value and the reciprocal of EC50). The results suggested that the phenolic compounds contributed significantly to the antioxidant capacity of R. carnea and T. chinensis.
研究领域:
植物化学
·
药理学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Chen Xiaoqiang,Yang Shihan,Yang Hong,Zhang Jinshan,Huang Yuyuan,Zhang Ying
Response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) and total phenols (TP) from Berberis kongboensis fruit. Single-factor experiments were conducted with the ultrasonication power, liquid–solid ratio, ethanol concentration, extraction temperature, and extraction time. A Plackett–Burman design was used to identify the main factors affecting the extraction rates of TF and TP. A Box–Behnken design was used to further optimize the ethanol concentration, solvent/sample ratio, and extraction temperature. Under the optimum conditions, the ethanol concentration of 34.67%, solvent/sample ratio of 38.44 mL/g, and extraction temperature of 46.53 °C, the yields of TF and TP were 107.92 mg rutin equivalents/g and 4.86 mg gallic acid equivalents/g, respectively. The extract from UAE had a better yield and higher antioxidant activities in vitro than that obtained by conventional solvent extraction. The B. kongboensis fruit is rich in flavonoids and phenolics, and it has potential application value in the food industry.
研究领域:
植物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Xiao-Qiang Chen,Zi-Han Li,Zhong-Juan Wang,Ling-Ling Liu,Tong-Tong Sun,Jian-Zhang Ma,Ying Zhang
Ultrasound-assisted extraction (UAE) of total anthocyanin (TA) and total phenolics (TP) from Rubia sylvatica Nakai fruit (RSF) was optimized using response surface methodology. On the basis of a single-factor experiment, six factors were screened using a Plackett-Burman experimental design to determine the variables that significantly influence the extraction yield of TA and TP, namely ultrasound power, liquid: solid ratio, ethanol concentration, pH value, extraction temperature, and extraction duration. Based on statistical significance analysis, ethanol concentration, pH value, and extraction temperature were selected for further optimization of the extraction process using a Box–Behnken design. Under the optimum conditions, yields of TA and TP were 22.50 ± 0.89 mg cyanidin-3-glucoside/g RSF and 36.20 ± 0.11 mg gallic acid/g RSF, respectively. The extract prepared under UAE exhibited excellent DPPH radical scavenging activity (IC 50 1.21 ± 0.04 mg/mL). The results demonstrate that R. sylvatica fruit are rich in anthocyanins and show considerable potential as a source of colorants for use in the food industry.
研究领域:
植物学
·
食品科学
·
天然产物化学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Richard Osae,Maurice Tibiru Apaliya,Emmanuel Kwaw,Michelle T.R. Chisepo,Otu Phyllis Naa Yarley,Eric Aforo Antiri,Raphael N. Alolga
This work aimed to determine the effects of five drying methods on the quality and essential oil composition of Ghanaian ginger. To this end, slices of fresh ginger rhizomes were dried using the following dryers; relative humidity convective (RHC) dryer, freeze (F) dryer, infrared (IR) dryer, microwave (MC) dryer and pulsed vacuum (PV) dryer. The antioxidant activities, total phenolic content (TPC), sensory characteristics and moisture content of the dried slices were assessed. The effects of the various drying techniques on the chemical composition of the essential oil components were determined using gas chromatography-mass spectrometry (GC–MS). With respect to the sensory attributes of the various dried ginger samples, the RHC-dried samples attained the highest scores for aroma, flavor and general acceptability while the F-dried samples scored the highest marks for appearance (i.e., colour). The RHC-dried samples had the lowest moisture content (5.45 %) followed by the F-dried (6.36 %), IR-dried (8.06 %), MC-dried (9.15 %) and PV-dried (10.07 %) samples. The F-dried samples retained the highest TPC (110.36 mg Gallic acid equivalent (GAE)/g db) and exhibited the highest antioxidant activities as evidenced by the outcomes of 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power capacity (FRAP) tests. The chemical profiles of the essential oil extracted from the various dried ginger samples were distinct and unique. The essential oil obtained from the F-dried samples was observed to have the highest compounds (24). The number of compounds identified in the oil extracted from the other dried samples were : RHC drying, 23; IR drying, 16; MC drying, 15; PV drying, 3. In general, the samples subjected to F and RHC drying gave the best outcomes for all the quality parameters assessed and are therefore recommended for large-scale industrial application.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Mengyang Hou,Chengyuan Lin,Lin Zhu,Zhaoxiang Bian
This study aimed to enhance the valorization of Chaenomeles speciosa leaves as a sustainable source of bioactive phenolics. An innovative ionic liquid-based ultrasound-assisted extraction (IL-UAE) method was developed for extracting phenolic compounds. Among 10 structurally diverse ILs, [BMIM]Br demonstrated superior extraction performance. Using a combination of single-factor design and response surface methodology (RSM), the optimal parameters for IL-UAE were determined to be the [BMIM]Br concentration of 1.33 mol/L, ultrasonic power of 380 W, extraction time of 10 min, and liquid-to-solid ratio of 22 mL/g. Under these conditions, the yield of C. speciosa leaves total phenolics (CSL-TP) was 78.14 ± 0.35 mg/g, which was substantially higher than those obtained via conventional heat reflux and UAE. After extraction, the microstructures of C. speciosa leaves were examined using scanning electron microscopy (SEM), which confirmed the effectiveness of IL-UAE. Subsequently, NKA-II resin column chromatography was developed to effectively purify crude CSL-TP extracts, guided by leakage and elution curve evaluations, yielding phenolic extracts with a purity of 75.40 % ± 1.93 %. A UPLC–QqQ–MS/MS method was developed for the quantitative analysis of nine major phenolics in purified CSL-TP extracts. Furthermore, bioactivity assessments demonstrated that the purified CSL-TP extracts efficiently scavenged radicals and effectively inhibited the proliferation of HCT-116 and HT-29 cell lines. These results highlight the potential of C. speciosa leaves as a valuable resource for the pharmaceutical and food industries, paving the way for the development of innovative therapeutic products and functional foods.
研究领域:
植物化学
·
分析化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2016
·Vol. 17
·期 5
·391
·
IF 1.303
Yun Xing,Le Cai,Tian-peng Yin,Yang Chen,Jing Yu,Ya-rong Wang,Zhong-tao Ding
The antioxidant activities and total phenolic content of fermented Salvia miltiorrhiza with fungus Geomyces luteus were investigated. The results revealed that G. luteus fermentation could significantly improve the antioxidant activity and total phenolic content of S. miltiorrhiza . The main antioxidant constituents were characterized by spectroscopic analysis as salvianolic acids. High-performance liquid chromatography (HPLC) quantification also showed the enhanced content of salvianolic acid B after fermentation. The present study suggests that G. luteus fermentations are effective in the S. miltiorrhiza salvianolic acids’ enrichment process.
研究领域:
药用植物学
·
天然产物化学
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Guan-Lin Chen,Song-Gen Chen,Yi Xiao,Nan-Lin Fu
In order to find new sources of natural antioxidants, total phenolic content (TPC) and total flavonoid content (TFC) of 30 flowers in their free, esterified and insoluble-bound forms were determined. Rosa rugosa Thunb (pink) showed the highest TPC, and Osmanthus fragrans had the highest TFC. The antioxidant activities of 30 flowers were determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) assays. Rosa rugosa Thunb (purple) and Rosa rugosa Thunb (pink) had the highest DPPH (612.79 and 544.75 μmol Trolox/g DW), FRAP (273.10 and 301.14 μmol Trolox/g DW) and TEAC (1013.71 and 937.19 μmol Trolox/g DW) value. Furthermore, several phenolic compounds were detected using ultra performance liquid chromatography (UPLC) technique, and (+)-catechin, ferulic acid, isoquercitrin, and quercitrin were widely found in these flowers. The results implied that the flowers were important natural sources of bioactive components with higher antioxidant capacities for use in the food and pharmaceutical industries.
研究领域:
植物学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 37
·24242-24251
·
IF 4.036
Xiaoxia Duan,Xueke Wang,Jiangwei Chen,Guijiang Liu,Yun Liu
In order to investigate the solubility behavior of lignin in formic acid (FA) solution Phragmites australis biomass was subjected to a sequential two-step formosolv fractionation using 88% FA followed by 70% FA to obtain four specific lignin fractions, designated as IFL-88%, IFSL-70%, IFIL-70% and IFL-EtAc. The structures of the four isolated lignin fractions were successfully characterized by gel permeation chromatography (GPC), Fourier transform infrared (FTIR) spectroscopy, two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy (2D-HSQC NMR), thermogravimetric analysis (TGA), and gas chromatography-mass spectroscopy (GC/MS). Furthermore, the total phenolic content of the four isolated lignin samples was assessed by Folin-Ciocalteu analysis. The data from structural properties revealed that depolymerization of the isolated lignin fractions occurred via β-O-4 cleavage, accompanied by competitive condensation reaction. Interestingly, 70% aqueous FA could separate specific lignin fractions with different antioxidant capacities of ABTS˙+ and DPPH radical scavenging activity. Due to the high total phenolic hydroxyl content (25%) and low molecular weight (Mw = 2760 Da) and polydispersity index (PDI = 1.5), IFL-EtAc lignin showed excellent antioxidant activity at the same concentration of 2.0 mg mL−1 in comparison with three other isolated lignin fractions, and it was even equal to that of commercial antioxidant butylated hydroxytoluene (BHT). These findings are helpful to separate specific lignins with higher value as potential antioxidants by sequential two-step formosolv fractionation in lignin chemistry.
研究领域:
植物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2019
·Vol. 85
·期 1
·123-131
·
IF 2.081
Chaojun He,Ziyong Li,Hongxia Liu,Haining Zhang,Liye Wang,Hong Chen
Adlay oil (AO) is an important component of adlay seeds that has many beneficial functions to human health. In this study, the variations in the chemical composition and antioxidant activity of AOs extracted from adlay samples obtained from four main producing areas of China were first investigated. Results revealed that the AO of the adlay samples varied from 7.398 ± 0.486% to 8.464 ± 0.725%. The highest contents of total fatty acid, triolein, total phenolic, and total flavonoid were observed in Xingren AO, whereas coixol content was found to be highest in Pucheng AO. The AO samples were grouped successfully in accordance with the origins by partial least squares–discriminant analysis and hierarchical cluster analysis based on the contents of the chemical components. Furthermore, AOs exhibited considerable levels of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (IC 50 , 0.924 ± 0.113 to 1.116 ± 0.109 mg/mL) radical scavenging activity, ferric-reducing antioxidant power (FRAP) activity (EC 50 , 0.019 ± 0.002 to 0.028 ± 0.002 mg/mL) activity, and β-carotene-linoleic acid bleaching activity (IC50, 0.233 ± 0.008 to 0.414 ± 0.012 mg/mL) activity. Total phenolic and total flavonoid both demonstrated highest correlation with ABTS values ( r = −0.952 and r = −0.960, respectively). The results derived from above studies suggest that geographic origin has a certain influence on the phytochemical profiles and antioxidant activity of the AOs. Practical Application The chemical composition and antioxidant activity of adlay oils extracted from the adlay samples obtained from four main producing areas of China were evaluated, including total fatty acid, triolein, total phenolic, and total flavonoid, and ABTS radical scavenging activity, FRAP activity, and β-carotene-linoleic acid bleaching activity.
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）