MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Fei Cheng,Taiming Zhang,Chunhua Yang,Hongqiu Zhu,Yonggang Li,Tao Sun,Can Zhou
To determine the total iron concentration in aqueous solutions containing complexing matrix, a direct and rapid ultraviolet–visible spectroscopy determination method was developed using the Nitroso R salt as a chromogenic reagent. In the presence of NaI and CTMAB, the experiment shows that Nitroso R salt-Fe complex can produce a significantly sensitive and selective absorbance peak at 710 nm with no notable time for sensing. The experimental factors that affect the procedure such as temperature, acidity and dosages of various reagents were analyzed and optimized. Under the optimized experimental conditions, the limit of detection and limit of quantification of the method was 4.5 × 10 −8 and 1.5 × 10 −7 mol/L. The calibration curve was obtained in a wide linear range of 1.5 × 10 −7 -6.4 × 10 −5 mol/L with good linearity (R 2 = 0.9989). The method was free of any interference with common ions and can determine the iron content in the complex matrix of aqueous samples. The methodology was further validated by the certified reference water samples used for environmental monitoring and agreed with the ICP-OES spectrophotometry results. Compared with the conventional method, the relative standard deviations of the spike test in various actual samples were in the range of 0.31%-2.9% ( n = 5). Good recoveries between 96.4% and 107% were obtained. The proposed method provides great promise for application in-field analysis of iron content in various environmental samples and hydrometallurgical solutions owing to its high sensitivity, low cost, good reproducibility, and no need for any complex pretreatment.
研究领域:
分析化学
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环境科学
·
冶金工程
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Kaige Zhang,Rong Guo,Yunhe Wang,Qiujun Nie,Guifen Zhu
In our work, hydrophobic thymol-based deep eutectic solvents (DESs) with strong reducibility, a lower density than water, and a slightly higher melting point than room temperature were synthesized. Based on these solid DESs, one-step derivatization and temperature-controlled vortex-assisted liquid-liquid microextraction based on the solidification of a floating DES (TC-VA-LLME-SFDES) via UV–Vis spectrophotometry for the rapid determination of total iron was developed. The derivatization and TC-VA-LLME were carried out simultaneously by the addition of DES as both the reducing agent and extraction solvent. After optimization, the calibration curve (5–250 μg L −1 , R 2 = 0.9982), limit of detection (1.5 μg L −1 ), limit of quantitation (5.0 μg L −1 ), precision (≤4.0%) and enrichment factor (92) was obtained. This method was applied for the determination of total iron in water and food samples with satisfactory recoveries (85.4–106.2%). One-step derivatization and TC-VA-LLME only required 2 min. Furthermore, this method opened up the application of solid DESs in liquid–liquid microextraction.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-9
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IF 4.122
Zhu Yun,Hu Xueping,Pan Dawei,Han Haitao,Lin Mingyue,Lu Yan,Wang Chenchen,Zhu Rilong
In this study, the speciation of iron (Fe), including total Fe (TFe) and acidified dissolved Fe (ADFe), was assessed by fast cathodic absorption stripping voltammetry, using a gold electrode and 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) as a novel complexing agent for Fe. The validity and accuracy of this method were compared with the standard spectrophotometry and tested by the standard samples. Under optimized conditions, the Fe response was linear within the range of 0.01 to 1 μM with a detection limit of 1.2 nM. To further validate this method, the variation in concentrations of TFe and ADFe were investigated at twelve sampling stations in a local coastal river, in both the dry and wet season. Additionally, to further understand the interaction between Fe and environmental factors, the relationships between the concentration of Fe species and dissolved oxygen (DO) and salinity were also discussed.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2019
·
IF 2.994
Chunhua Li,Li Zhu,Weixia Yang,Xie He,Haiping Zhao,Wenzhi Tang,Tianli Yue,Zhonghong Li
It is imperative to exploit simple and rapid methods for the detection of total iron in foods to ensure food quality and human health. Herein, we construct the MIL-53(Al)-NH 2 -MPA nanosensor synthesized by post-synthetic modification process for fluorescent detection of total iron in food matrix. The nanosensor emits intense blue fluorescence with excellent stability. The fluorescence can be efficiently quenched by Fe(III) due to the fact that Fe(III) can preferentially bind with O-quinone bands or –NH 2 of MIL-53(Al)-NH 2 -MPA to form strong coordination complex, and that the heteroatoms (N, O and S) in MIL-53(Al)-NH 2 -MPA can donate lone-pair electrons to Fe(III) to promote the electron transfer. Moreover, the competitive absorption of excitation energy of MIL-53(Al)-NH 2 -MPA by Fe(III) endows the nanosensor with superior sensitivity. The fluorescent sensor exhibits excellent anti-interference performances toward other metal ions, and reveals a LOD of 0.36 μM with the broad linear range of 0–110 μM for the detection of Fe(III). Satisfactory recoveries of 92.90–112.70% with RSDs of 0.54–2.48% have been achieved in water and food samples, which are well agreement with inductively coupled plasma mass spectrometry (ICP-MS) (P > 0.05), indicating that MIL-53(Al)-NH 2 -MPA is applicable for detecting total iron in food matrix.
研究领域:
分析化学
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食品科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Nutrition & Metabolism
·
2022
·Vol. 19
·期 1
·1-9
·
IF 4.654
Qiu Fubin,Li Rui,Gu Siyu,Zhao Yimin,Yang Linxue
Background Iron and vitamin D (VD) is essential to health. Previous studies have shown that iron homeostasis has a potential effect on VD metabolism, but the mechanism is not fully understood.ObjectivesTo explore the relationship between VD metabolism and iron metabolism, as well as the regulatory mechanism of iron on VD metabolism.Methods 40 male rats were fed adaptively for 7 days and randomly divided into control (C, n = 6 normal diet) group and model (M, n = 24 iron deficient diet) by simple randomization, the latter was used to establish iron deficiency anemia (IDA) model. After 6 weeks of feeding, the M group was randomly divided into: iron deficiency group (DFe), low iron group (LFe), medium iron group (MFe) and high iron group (HFe) by block randomization. Different doses of iron dextran (based on iron content (100 g·bw·d)): 0, 1.1, 3.3 and 9.9 mg) were given respectively. After 4 weeks, the rats were anesthetized with 8% chloral hydrate, Blood (collected from the abdominal aorta), liver and kidney tissues were collected. The serum and tissues were separately packed and frozen at -80℃ for testing.Results The results showed that the levels of hemoglobin (Hb), red blood cell (RBC), serum iron (SI), liver iron, and kidney iron in DFe group were lower than those in the other four groups, while the levels of total iron-binding capacity (TIBC), transferrin (TF) and transferrin receptor (Tfr) in DFe group were higher than those in other groups; The serum levels of 25-(OH)D3 and 1,25-(OH)2D3 in DFe group were significantly lower than those in C group (P < 0.05). The correlation analysis showed that the levels of 25-(OH)D3 and 1,25-(OH)2D3 were negatively correlated with TIBC, TF and Tfr no correlation with SI. Western blotting, immunofluorescence, and q-PCR results showed that compared with C group, the protein and gene expressions of CYP2R1, CYP27A1, and CYP24A1 in DFe group were down-regulated, and the expression of CYP27B1 protein and gene was up-regulated in DFe group.Conclusion Iron may be involved in the metabolism of VD3 by regulating the expression of VD3 hydroxylase, suggesting that appropriate iron supplementation might promote the activation of VD3.
研究领域:
营养学
·
生物化学
·
药理学
·
动物生理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）