Food Chemistry-X
·
2024
·
IF 6.5
Xuyan Zong,Xianjiang Zhou,Xinyue Cao,Shun Gao,Dongyang Zhang,Haoran Zhang,Ran Qiu,Yi Wang,Jianhang Wu,Li Li
Craft beer brewers need to learn process control strategies from traditional industrial production to ensure the consistent quality of the finished product. In this study, FT-IR combined with deep learning was used for the first time to model and analyze the Plato degree and total flavonoid content of Qingke beer during the mashing and boiling stages and to compare the effectiveness with traditional chemometrics methods. Two deep learning neural networks were designed, the effect of variable input methods on the effectiveness of the models was discussed. The experimental results showed that the CARS-LSTM model had the best predictive performance, not only as the best quantitative model for Plato in the mashing (R 2 p = 0.9368) and boiling (R 2 p = 0.9398) phases but also as the best model for TFC in the boiling phase (R 2 p = 0.9154). This study demonstrates the great potential of deep learning and provides a new approach to quality control analysis in beer brewing.
研究领域:
食品科学
·
化学计量学
·
人工智能
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Lei Liu,Wei Wen,Ruifen Zhang,Zhencheng Wei,Yuanyuan Deng,Juan Xiao,Mingwei Zhang
In this study, phenolic profiles and antioxidant activity of rice bran were analyzed following successive treatment by gelatinization, liquefaction and complex enzyme hydrolysis. Compared with gelatinization alone, liquefaction slightly increased the total amount of phenolics and antioxidant activity as measured by ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. Complex enzyme hydrolysis significantly increased the total phenolics, flavonoids , FRAP and ORAC by 46.24%, 79.13%, 159.14% and 41.98%, respectively, compared to gelatinization alone. Furthermore, ten individual phenolics present in free or soluble conjugate forms were also analyzed following enzymatic processing. Ferulic acid experienced the largest release, followed by protocatechuic acid and then quercetin. Interestingly, a major proportion of phenolics existed as soluble conjugates , rather than free form. Overall, complex enzyme hydrolysis releases phenolics, thus increasing the antioxidant activity of rice bran extract. This study provides useful information for processing rice bran into functional beverage rich in phenolics.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Chen Xiaoqiang,Yang Shihan,Yang Hong,Zhang Jinshan,Huang Yuyuan,Zhang Ying
Response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) and total phenols (TP) from Berberis kongboensis fruit. Single-factor experiments were conducted with the ultrasonication power, liquid–solid ratio, ethanol concentration, extraction temperature, and extraction time. A Plackett–Burman design was used to identify the main factors affecting the extraction rates of TF and TP. A Box–Behnken design was used to further optimize the ethanol concentration, solvent/sample ratio, and extraction temperature. Under the optimum conditions, the ethanol concentration of 34.67%, solvent/sample ratio of 38.44 mL/g, and extraction temperature of 46.53 °C, the yields of TF and TP were 107.92 mg rutin equivalents/g and 4.86 mg gallic acid equivalents/g, respectively. The extract from UAE had a better yield and higher antioxidant activities in vitro than that obtained by conventional solvent extraction. The B. kongboensis fruit is rich in flavonoids and phenolics, and it has potential application value in the food industry.
研究领域:
植物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Yinghua Xie,Yunxia Zheng,Xiling Dai,Quanxi Wang,Jianguo Cao,Jianbo Xiao
The seasonal dynamics of the total flavonoid contents in various parts of Dryopteris erythrosora , a traditional Chinese medicinal fern, and their antioxidant activity were investigated. The total flavonoids content in various parts of D. erythrosora showed an obvious seasonal dynamic change. The total flavonoid contents in stems (from 4.3% to 12.5%) were much higher than that in leaves with an average content of 2.01%. In spring, the total flavonoid contents in stems were relatively low, but increased rapidly from summer to winter. However, the seasonal dynamics of total flavonoid contents in leaves showed different model. The total flavonoid contents in the stems showed a negative correlation with that in the leaves from January to July. The correlation coefficient of about −0.7 was obtained. The antioxidant activity of the extracts also altered in proportion to the change of total flavonoid contents. In general, the extracts from stems always showed highest antioxidant potentials and it was suggested that the stems can be used as crude medicine.
研究领域:
植物学
·
药理学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Guan-Lin Chen,Song-Gen Chen,Yi Xiao,Nan-Lin Fu
In order to find new sources of natural antioxidants, total phenolic content (TPC) and total flavonoid content (TFC) of 30 flowers in their free, esterified and insoluble-bound forms were determined. Rosa rugosa Thunb (pink) showed the highest TPC, and Osmanthus fragrans had the highest TFC. The antioxidant activities of 30 flowers were determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) assays. Rosa rugosa Thunb (purple) and Rosa rugosa Thunb (pink) had the highest DPPH (612.79 and 544.75 μmol Trolox/g DW), FRAP (273.10 and 301.14 μmol Trolox/g DW) and TEAC (1013.71 and 937.19 μmol Trolox/g DW) value. Furthermore, several phenolic compounds were detected using ultra performance liquid chromatography (UPLC) technique, and (+)-catechin, ferulic acid, isoquercitrin, and quercitrin were widely found in these flowers. The results implied that the flowers were important natural sources of bioactive components with higher antioxidant capacities for use in the food and pharmaceutical industries.
研究领域:
植物学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Li Yang,Fengxiang Zhang,Weiwei He,Boyuan Zhao,Ting Zhang,Shang Wang,Lifen Zhou,Junwei He
Ethnopharmacological relevance Hosta plantaginea (Lam.) Aschers flowers (HPF) are well-known for their high flavonoid content, which contribute to their widely as traditional Chinese medicine for alleviating inflammation. Despite their recognized potential, information regarding the total flavonoid (TF) of HPF and its therapeutic application in treating chronic prostatitis (CP) remains unknown. Aim of the study We aimed to investigate the extraction optimization, constituent analysis, and alleviating effect of TF on CP as well as its potential mechanism. Materials and methods The optimized extraction of TF from HPF was explored using response surface methodology with a Box-Behnken design model. The major flavonoids in TF were identified based on UHPLC-MS approach. Efficacy of TF (25 and 100 mg/kg, p.o.) on CP was evaluated in prostate antigen emulsion-induced autoimmune CP rat model by measuring prostatic index, the levels of leukocytes and lecithin bodies, as well as histopathological examination. The protein expression contents were detected by western blotting . Additionally, the antioxidant (DPPH and ABTS) and anti-inflammatory (cyclooxygenase 2, COX-2 inhibitory) effects of TF were also evaluated in vitro . Results The optimized conditions for TF extraction were determined as 60% ethanol concentration, 30 mL/g liquid-to-solid ratio, 30 min extraction time, and 90 °C extraction temperature, and the extraction ratio is 65.98 ± 2.14%. A total of 15 major flavonoids in TF were characterized by comparison with reference standards. TF ameliorated the efficacy of CP in rats in a dose-independent manner, including reduced prostatic index and leukocytes levels, elevated lecithin body levels, ameliorated histopathological damage to prostate, and suppressed phosphorylated protein expressions of nuclear factor kappa-B (NF-κB) p65, inhibitor of NF-κB alpha (IκBα), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (Erk), just another kinase 1 (JAK1), signal transducer and activator of transcription 3 (STAT3), phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). Simultaneously, the IC 50 of TF to DPPH, ABTS radicals, and COX-2 were 2.02, 1.79, and 0.0838 mg/mL, respectively. Conclusions We first demonstrated that TF from HPF represents a promising candidate to alleviate CP through suppression of NF-κB, MAPKs, JAK-STAT, and PI3K-Akt signaling pathways.
研究领域:
药理学
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植物化学
·
炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2016
·
IF 3.299
Peng Huang,Qing Zhang,Hongye Pan,Lianjun Luan,Xuesong Liu,Yongjiang Wu
In the present study, integrated extraction-adsorption process was developed and applied to extract and purify total flavonoids from Scutellariae barbatae herba. Screening of macroporous resins, single factor experiments, an experimental design based on Box-Behnken design (BBD) and purification conditions study were carried out to optimize the integrated process. DM130 resin was chosen as adsorbent and the optimal extraction-adsorption conditions were determined as pH of extraction solvent 2, time 9 h, liquid/solid ratio 20:1 mL/g and flow rate 7 BV/h. 70% (5 BV) ethanol aqueous solution was selected as elution solvent. Through this procedure, the total flavonoids of Scutellariae barbatae herba can be totally extracted using water as extraction solvent rather than high concentration ethanol was used in traditional extraction method. Moreover, the integrated process can simplify operation, reduce energy consumption, and prevent the degradation of bioactive substances, thus improve the quality of products. Compared to the conventional method, the recovery yield and purity of total flavonoids obtained from this procedure increased by 1.50 and 1.16 times respectively. The results showed that the integrated procedure can be efficient and eco-friendly, as well as appropriate for large-scale extraction and purification of total flavonoids from Scutellariae barbatae herba .
研究领域:
中药学
·
天然产物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2019
·Vol. 85
·期 1
·123-131
·
IF 2.081
Chaojun He,Ziyong Li,Hongxia Liu,Haining Zhang,Liye Wang,Hong Chen
Adlay oil (AO) is an important component of adlay seeds that has many beneficial functions to human health. In this study, the variations in the chemical composition and antioxidant activity of AOs extracted from adlay samples obtained from four main producing areas of China were first investigated. Results revealed that the AO of the adlay samples varied from 7.398 ± 0.486% to 8.464 ± 0.725%. The highest contents of total fatty acid, triolein, total phenolic, and total flavonoid were observed in Xingren AO, whereas coixol content was found to be highest in Pucheng AO. The AO samples were grouped successfully in accordance with the origins by partial least squares–discriminant analysis and hierarchical cluster analysis based on the contents of the chemical components. Furthermore, AOs exhibited considerable levels of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity (IC 50 , 0.924 ± 0.113 to 1.116 ± 0.109 mg/mL) radical scavenging activity, ferric-reducing antioxidant power (FRAP) activity (EC 50 , 0.019 ± 0.002 to 0.028 ± 0.002 mg/mL) activity, and β-carotene-linoleic acid bleaching activity (IC50, 0.233 ± 0.008 to 0.414 ± 0.012 mg/mL) activity. Total phenolic and total flavonoid both demonstrated highest correlation with ABTS values ( r = −0.952 and r = −0.960, respectively). The results derived from above studies suggest that geographic origin has a certain influence on the phytochemical profiles and antioxidant activity of the AOs. Practical Application The chemical composition and antioxidant activity of adlay oils extracted from the adlay samples obtained from four main producing areas of China were evaluated, including total fatty acid, triolein, total phenolic, and total flavonoid, and ABTS radical scavenging activity, FRAP activity, and β-carotene-linoleic acid bleaching activity.
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2025
·
IF 2.8
Walaa Kobacy,Ahmed K. Rashwan,Ahmed I. Osman,Ashraf K. Abu-Zaid,Ahmed R. Mazeed,Reda M. Mohamed
This study investigates the physicochemical, antioxidant, and anticancer properties of four Egyptian honey types: clover, fennel, sidr, and multiflower. These honeys exhibited significant variability in color, pH, acidity, sugars, and enzymatic activity. The fennel honey demonstrated the highest content of total phenolic (TPC) of 238.76 mg GAE/100 g, and the total flavonoid (TFC) was 94.06 mg QE/100 g. In addition, the antioxidant activity as measured by DPPH radical scavenging correlated strongly with bioactive compound concentrations, with fennel honey showing the highest inhibition (60.14%). In vitro cytotoxicity assays against Hep-2 human laryngeal cancer cells revealed fennel honey to have the lowest IC50 value (70 μg/mL), followed by multiflower honey (IC50 = 87 μg/mL), confirming a significant relationship between the TPC, TFC, and the anticancer effects. This highlights the role of bioactive compounds in honey as potential natural anticancer agents. Clover honey exhibited the weakest cytotoxicity (IC50 = 130 μg/mL) with the lowest TPC and TFC. These findings underscore the potential of Egyptian honey varieties, particularly fennel and multiflower honeys, as promising natural sources of antioxidants and anticancer agents, suggesting a beneficial therapeutic role in cancer prevention. Further studies are needed to elucidate the detailed mechanisms by which honey compounds exert these anticancer effects.
研究领域:
食品科学
·
肿瘤学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Asian Biomedicine
·
2020
·Vol. 14
·期 1
·19-26
·
IF 0.324
Luyun Yan,Yulei Xie,Youyou Wang,Jinhua Zhu,Mingjing Li,Xiuhua Liu,Dongbao Zhao
BackgroundLonicera japonica Thunb has been comprehensively used as a traditional Chinese medicine which contains a variety of medicinally active substances.ObjectivesThe active constituents in various parts of L. japonica Thunb (leaves [LE], flowers fl, and green buds [GB]) were studied to rational and efficient use of this medicinal plant.MethodsUV–vis spectrophotometry was used for the determination of the total flavonoids; gas chromatography–mass spectrometric method was employed for the analysis of volatile components; and high-performance liquid chromatography technology was applied to detect nine bioactive components including chlorogenic acid, neochlorogenic acid, isochlorogenic acid C, caffeic acid, luteolin, galuteolin, hyperoside, rutin, and quercetin in different parts of L. japonica Thunb. The antimicrobial effects of LE, FL, and GB were further measured using agar plate drilling method.ResultsThe content of the total flavonoids in different portions of L. japonica Thunb has significant difference, and the content with the decreasing sequence is found in GB, LE, and FL. The contents of main volatile components were highest in FL, followed by GB and LE. Besides, the contents of galuteolin, luteolin, rutin, and isochlorogenic acid C were higher in LE than those in FL and GB, and neochlorogenic acid content was similar in LE and FL. The antimicrobial effects showed
研究领域:
中药学
·
植物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2014
·Vol. 52
·期 4
·2458-2463
·
IF 2.024
Zhou Xiaoli,Hao Tingfeng,Zhou Yiming,Tang Wen,Xiao Ying,Meng Xiaoxiao,Fang Xiang
Relationships of changes between major non-enzymatic antioxidant compounds and antioxidant capacities of tartary buckwheat during germination were evaluated by means of correlation analysis and principal component analysis in this paper. The changes of antioxidant compounds, including vitamin C, vitamin E, flavonoids, carotenoids, and chlorophyll, and antioxidant activities were detected. A good accumulation in the content of vitamin C (0.71 mg/g), total flavonoids (19.53 mg rutin/g), and rutin (11.34 mg/g) was found after 7-day germination, but germination decreased the vitamin E activity. Germination improved the activities of buckwheat extracts to scavenge DPPH, ABTS, and superoxide free radicals by 107, 144, and 88 %, respectively. Furthermore, the correlation and principal component analysis showed that the vitamin C, total flavonoids, and rutin contents were closely related positively with free radicals scavenging properties, indicating that the compounds which play a key role in the elevated antioxidant activities during germination consisted of vitamin C, total flavonoids, and rutin, but not vitamin E and quercetin.
研究领域:
食品科学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2021
·Vol. 2021
·期 1
·2894306
·
IF 2.63
Hong Chang,Shujie Lv,Tengteng Yuan,Huan Wu,Lei Wang,Ran Sang,Caiyun Zhang,Weidong Chen
Gushuling (GSL), a well-known hospital preparation composed of traditional Chinese medicine (TCM), has been widely used in the clinical treatment of osteoporosis (OP) for decades due to its remarkable therapeutic effect. However, the chemical constituents of GSL are still unclear so far, which limits the in-depth study of its pharmacodynamic material basis and further restricts its clinical application. In this study, we developed a strategy for qualitative analysis of the chemical constituents of GSL in vitro and in vivo . Based on the results of ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and the UNIFI informatics platform, the chemical constituents of GSL can be determined quickly and effectively. By comparing the retention time, accurate mass, and fragmentation spectrum of the compounds in GSL, a total of 93 compounds were identified or preliminarily identified, including flavonoids, terpenoids, phenylpropanoids, steroids, etc. Among them, nine compounds have been confirmed by standard substances, namely epimedin A, epimedin B, epimedin C, icariin, ecdysterone, calycosin, calycosin-7-glucoside, ononin, and ginsenoside Ro. Fragment patterns and characteristic ions of representative compounds with different chemical structure types were analyzed. At the same time, 20 prototype compounds and 42 metabolites were detected in rat serum. Oxidation, hydration, reduction, dehydration, glutathione S-conjugation, and acetylcysteine conjugation were the main transformation reactions of GSL in rat serum. In this research, the rapid method to characterize the in vitro and in vivo chemical constituents of GSL can not only be used for the standardization and quality control of GSL but also be helpful for further research on its pharmacodynamic material basis.
研究领域:
中药学
·
分析化学
·
药理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）