ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Xiaomeng Zhou,Saijin Huang,Wenfeng Guo,Wenfeng Liu,Mengyao Wen,Li Shang
The simultaneous detection of multiple bioanalytes with similar structures has been a long-standing challenge for biological research and disease diagnosis. Bioreceptor-inspired sensor arrays provide an attractive and competitive solution for addressing this challenge, but applying this technique to biosensing in practical application scenarios is complicated by many factors such as the lack of robust probes, interference from the biological matrix, and difficulty for on-site analysis. In this work, by taking advantage of the intrinsic fluorescence and enzyme-mimic properties of gold nanoclusters (AuNCs), we report the design of an innovative ratiometric sensor array toward enhanced fluorescent visual biosensing. With biologically important phosphates as an example, we show that the present fluorescent clusterzyme-based ratiometric sensor array could effectively discriminate and detect eight types of phosphates. In particular, AuNCs with three different emission colors (blue, green, and red) and good peroxidase-mimic properties were employed as the sensing units, and the presence of phosphates affected both the intrinsic fluorescence and the enzymatic activity of these AuNCs, yielding distinct optical responses in a ratiometric manner. Moreover, a portable sensor array was established by further integrating these fluorescent clusterzymes into a hydrogel matrix, which could visually identify different phosphates based on their distinct fluorescence color changes. Consequently, the point-of-care diagnosis of urinary tract infections at different levels was achieved by analyzing urinary microbial ATP via the present strategy. This study illustrates the great potential of clusterzyme-based fluorescent sensor arrays as a promising biosensing platform for disease diagnosis.
研究领域:
生物传感
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
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2021
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IF 2.604
Ziheng Chen,Song Peng,Shixin Liu,Chengpeng Li,Xiang Cai,Dong Ma,Wu Zhang
Bacterial infection is a serious threat to human health. In this work, carbon dots (CDs) based on the polyethyleneimine (PEI) was prepared by hydrothermal carbonization method and then reacted with nitric oxide (NO) to form the NO-loaded CDs (CD-PEI/NONOate). CD-PEI/NONOate showed a high NO payload and outstanding fluorescence characteristics. It was found that CD-PEI/NONOate could inhibit the growth of Gram-negative bacteria Pseudomonas aeruginosa by more than 99%. Moreover, CD-PEI/NONOate showed no cytotoxicity to 3T3 cells at concentrations used in vitro. These results indicated that the prepared CD-PEI/NONOate has a potential application in the diagnosis and treatment of bacterial infection.
研究领域:
材料科学
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微生物学
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生物医学工程
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产品关联
DOI
高影响因子
Nano Today
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2022
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IF 18.962
Kai Yuan,Kai Huang,Yiqi Yang,Yixuan Lin,Yihao Liu,Fupeng Li,Yakun Liang,Haishuang Chang,Yuhui Chen,Tingting Tang,Shengbing Yang
Increasing evidence has emphasized the pivotal role of antibiotic tolerance in the evolution of antibiotic resistance , refractory infection and infection recurrence. Moreover, satisfactory treatment of infection relies highly on early, precise detection and subsequent timely therapeutic interventions. In this study, the phenotype of increased production of H 2 S in antibiotic-tolerant Staphylococcus aureus ( S. aureus ) was identified and proven to be essential to the maintenance of antibiotic tolerance. Hence, a photoacoustic probe for detecting infection as well as an antibiotic enhancer targeting bacterial endogenous H 2 S to eradicate antibiotic-tolerant bacteria was successfully developed based on a bismuth metal-organic framework (Bi-MOF). Bi-MOF achieved early in situ and precise photoacoustic imaging of infection by generating Bi 2 S 3 inside bacteria. Moreover, Bi-MOF enhanced the eradication efficacy of methicillin against tolerant S. aureus by downregulating the intracellular H 2 S levels. Overall, Bi-MOF offers a promising diagnostic tool for early, precise infection diagnosis and a therapeutic strategy for tolerant bacteria-related refractory infection.
研究领域:
纳米技术
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医学诊断
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抗菌治疗
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传染病
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产品关联
DOI
高影响因子
TALANTA
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2025
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IF 6.1
Tao Chen,Hongqin Guo,Xueyun Wen,Jinping Yu,Hao Shi,Lupeng Wang,Kaikai Xu,Yuanhong Xu
Endotoxin sensing has been receiving increasing attention as the endotoxin concentration is an important index for evaluating bacterial contamination and infection. The gold-standard sensing method based on horseshoe crab deformed cell lysate analysis (LAL) has been facing great challenges including high cost and animal ethical issues, which encourages researchers to explore alternative sensing probes to LAL. Herein, using 3-aminobenzoboric acid as a functional reagent, nitrogen and boron doped CeO 2 nanozymes with phosphatase activity are successfully designed and prepared. Interestingly, the introduced nitrogen and boron elements endows the nanozymes with obviously enhanced catalytic activity and high selectivity to endotoxin due to the specifical interaction between endotoxin and boric acid residue groups. Accordingly, the limit of detection of the as-prepared fluorescent liquid phase sensor is as low as 5.17 ng mL −1 within the linear range of 20–200 ng mL −1 . Impressively, the detection time of the fabricated sensor is only 10 min and the sensor shows extremely high selectivity for lipopolysaccharide, which exhibits great application potential as alternative to traditional endotoxin sensors.
研究领域:
分析化学
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纳米医学
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生物传感器
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感染诊断
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材料科学
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免疫学
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转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）