INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Shaochun He,Junting Li,Xundan Cao,Fei Xie,Hui Yang,Cheng Wang,Carla Bittencourt,Wenjiang Li
A novel reusable, high-compressible cotton regenerated cellulose/chitosan composite aerogel (RC/CSCA) was prepared using N -methylmorpholine-N-oxide (NMMO) as the green cellulose solvent, and glutaraldehyde (GA) as the crosslinking agent. The regenerated cellulose obtained from cotton pulp could chemically crosslink with chitosan and GA, to form a stable 3D porous structure. The GA played an essential role in preventing shrinkage and preserving the deformation recovery ability of RC/CSCA. Due to the ultralow density (13.92 mg/cm 3 ), thermal stability (above 300 °C), and high porosity (97.36 %), the positively charged RC/CSCA can be used as a novel biocomposite adsorbent for effective and selective removal of toxic anionic dyes from wastewater, showing an excellent adsorption capacity, environmental adaptability, and recyclability. The maximal adsorption capacity and removal efficiency of RC/CSCA for methyl orange (MO) was 742.68 mg/g and 95.83 %.
研究领域:
材料科学
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环境工程
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化学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Jian Wang,Fangming Chen,Qiaoyu Yang,Yao Meng,Mingrui Jiang,Yaqiong Wang,De-Wen Zhang,Liping Du
Multi-channel evaluation on a single sensor interface is highly valuable but full of challenge. Herein, a universal light-addressable electrochemical immunoassay based on silicon photoelectrodes was proposed for spatially-resolved detection of prostate-specific antigen (PSA) as a model target. To stabilize and functionalize silicon substrate, a simple and robust antifouling coating was prepared by cross-linking bovine serum albumin (BSA) matrix with glutaraldehyde (GA). For the PSA detection, an electrochemical sandwich-type immunosensor was performed on the basis of [email protected] functionalized silicon, in which horse radish peroxidase (HRP) was used to catalyze the reaction of H 2 O 2 and hydroquinone (HQ), resulting in in situ generation of benzoquinone (BQ). The light-induced local reduction photocurrent of BQ was then recorded as the sensor response by irradiating the target spot of photoelectrode respectively with a moveable light pointer. The results demonstrated the feasibility of localized measurements of enzymatic reactions . Coupling with light-addressable electrochemistry (LAE) and enzyme-linked immunosorbent assay (ELISA) protocol, multiplexed PSA detection could be achieved on a single sensor chip with good sensitivity and specificity. This proof-of-concept study provides a promising platform for high-throughput and accurate immunoanalysis in diagnostic or biodefense applications.
研究领域:
生物化学
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分析化学
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生物医学工程
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免疫学
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2021
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IF 3.935
Hui Jiang,Shi-Jun Yin,Xu Wang,Min Lu,Hao Zhang,Feng-Qing Yang
Two liposomes , including 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) + 1,2-dimyristoyl- sn -glycero-3-phosphoethanolamine (PE) + cholesterol (Chol) (DSPC/PE/Chol liposomes) and soybean lecithin (SPC) + PE + Chol (SPC/PE/Chol liposomes), were prepared and fixed on the inner wall of capillary column by using the adhesion of polydopamine (PDA) membrane and the cross-linking property of glutaraldehyde (GA). The immobilized liposome capillary column (ILCC) has good repeatability and stability based on the electrophoretic mobility of analyte . A CE method based on the immobilized liposome capillary column chromatography (ILCCC) was successfully developed to study the retention behavior of drugs on ILCC, and the logarithm of retention factor (log k ) of neutral and ionic drugs were determined. The results show that the log k measured by the ILCCC based on two liposomes have a good linear fitting (R 2 = 0.86). Moreover, the linear relationship between ILCCC system and other related research systems (octanol-water system and immobilized artificial membrane (IAM)) was analyzed, and the results indicate that SPC/PE/Chol ILCCC, DSPC/PE/Chol ILCCC and IAM systems have good fitting results, R 2 values are 0.86 and 0.78, respectively. In addition, the normalization coefficients of ILCCC and IAM systems obtained by the linear free energy relationship (LFER) analysis are close and the d value is small. In short, the ILCCC is a simple and feasible method for studying drug membrane permeability.
研究领域:
分析化学
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药学
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生物技术
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
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IF 6.1
Shubin Shi,Mengyue Hu,Xu Peng,Can Cheng,Shaoxiong Feng,Xinyun Pu,Xixun Yu
Due to the increasing aging population and the advancements in transcatheter aortic valve replacement (TAVR), the use of bioprosthetic heart valves (BHVs) in patients diagnosed with valvular disease has increased substantially. Commercially available glutaraldehyde (GA) cross-linked biological valves suffer from reduced durability due to a combination of factors, including the high cell toxicity of GA, subacute thrombus, inflammation and calcification. In this study, the oxidized chondroitin sulfate (OCS), a natural polysaccharide derivative, was used to replace GA to cross-link decellularized bovine pericardium (DBP), carrying out the first crosslinking of DBP to obtain OCS-BP. Subsequently, in situ the zwitterion radical copolymerization system was introduced to perform double cross-linking to obtain double crosslinked BHVs with biomimetic modification (P(APM/MPC)-OCS-BP). P(APM/MPC)-OCS-BP presented enhanced mechanical properties, collagen stability and enzymatic degradation resistance owing to double crosslinking. The ex vivo AV-shunt assay and coagulation factors test suggested that P(APM/MPC)-OCS-BP exhibited excellent anticoagulant and antithrombotic properties due to the introduction of P(APM/MPC). P(APM/MPC)-OCS-BP also showed good HUVECs-cytocompatibility due to the substantial reduction of its residual aldehyde group. The subcutaneous implantation also demonstrated that P(APM/MPC)-OCS-BP showed a weak inflammatory response due to the anti-inflammatory effect of OCS. Finally, in vivo and in vitro results revealed that P(APM/MPC)-OCS-BP exhibited an excellent anti-calcification property. In a word, this simply cooperative crosslinking strategy provides a novel solution to obtain BHVs with good mechanical properties, HUVECs-cytocompatibility, anti-coagulation, anti-inflammatory and anti-calcification. It might be a promising alternative to GA-fixed BP and exhibited a good prospect in the clinical applications.
研究领域:
心血管工程
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生物材料科学
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组织工程
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医疗器械
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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