ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Jingyuan Wang,Ronggang Zhai,Yifan Ma,Haoyu Chen,Danyang Jing,Huaiyu Yang,Yi Wang
Owing to their small size, morphology and release modification properties, nanopesticides are considered promising alternative strategies for enhancing biological activity and minimizing pesticide losses. In this study, we used a colloidal self-assembly method to develop a morphology-stable, regularly rod-shaped nanoselenium pesticide carrier (NSer), which was further modified with chitosan. After loading penthiopyrad (PEN), the biological activity of NSer@PEN and its impact on the physiological and biochemical processes of plants were further compared with those of spherical nanoselenium pesticides (NSes@PEN) and commercial materials (20 % PEN SC). The biological activities were quantified through the EC 50 values, which revealed that NSer@PEN (0.71 mg/L) and NSes@PEN (1.09 mg/L) exhibited significantly greater activity against Colletotrichum orbiculare Arx compared to 20 % PEN SC (2.70 mg/L). Moreover, through further investigation into the impact of nanopesticides on plant root exudates, Fourier transform infrared spectroscopy (FTIR) and two-dimensional correlation spectroscopy (2D-COS) analysis revealed that the ketone C O bond exhibited the strongest binding affinity, and the C O bond of phenols contributed significantly to the binding of cucumber root exudates induced by NSer@PEN, resulting in a mild response of the plant. The morphology-dependent nanoselenium carriers developed in this work are expected to enhance biological activity and reduce plant stress caused by pesticides, tackling one of the application challenges of pesticides.
研究领域:
植物生理学
·
农业科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2021
·Vol. 108
·期 2
·267-275
·
IF 2.151
Pan Lixiang,Feng Xiaoxiao,Jing Jing,Zhang Jingcheng,Zhuang Ming,Zhang Yun,Wang Kai,Zhang Hongyan
The mixed application of pesticides and foliar fertilizer has been widely used in the production of cucumber, however, their effects on plant growth and pesticide dissipation are still unclear. In this study, the effects of mixed application of pymetrozine, tebuconazole and foliar fertilizer on the cucumber plant growth and pesticide dissipation were investigated simultaneously. The results show that the mixed use of pymetrozine, tebuconazole, especially adding foliar fertilizer, improved the physiological indexes (i.e., area, nitrogen content and chlorophyll content of the leaves, and root growth) of cucumber plants compared to those with the application of single pesticide. Meanwhile, it can significantly affect the dissipation of pymetrozine even in the slower growth matrices (lower leaves, stems, and plants). The residue of tebuconazole in cucumber plants was affected by the combination of formulation type and foliar fertilizer. This study can provide data for scientifically guiding the mixed application of pesticide and fertilizer.
研究领域:
农业科学
·
植物保护
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2023
·Vol. 11
·期 4
·326
·
IF 4.6
Xiaoran Ma,Xin Chen,Haonan Hou,Donghui Liu,Xueke Liu,Peng Wang,Zhiqiang Zhou
As two commonly used fungicides, carbendazim and tebuconazole are widely found in the environment and in foods. Studies have reported that these fungicides can induce hepatic oxidative stress and other health risks. Nevertheless, the influences of exposure to carbendazim and tebuconazole at their acceptable daily intake (ADI) doses on hepatic oxidative stress, and the residual distributions in mice remain unclear. To fill these gaps, ICR (CD-1) mice were exposed to carbendazim and tebuconazole at their ADI doses by oral administration for 4 weeks in this study. The results showed that tebuconazole accumulated primarily in the epididymal fat of mice (16.84 μg/kg), whereas no significant residues of carbendazim in the tissues were observed. In addition, exposure to ADI doses of tebuconazole significantly reduced liver coefficients and induced hepatic oxidative stress in mice, including elevating the levels of glutathione and malonaldehyde. However, no significant impacts were observed on the hepatic redox homeostasis in mice after exposure to carbendazim at its ADI dose. The results could be helpful for understanding the exposure risks of carbendazim and tebuconazole in terms of low doses and long term.Keywords:carbendazim;tebuconazole;residue;oxidative stress;acceptable daily intake
研究领域:
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Kun Jia,Guilan Chen,Junquan Zeng,Fasheng Liu,Xinjun Liao,Chen Guo,Jiaqi Luo,Guanghua Xiong,Huiqiang Lu
Trifloxystrobin-tebuconazole (TFS-TBZ) is a novel, broad-spectrum fungicide that has been frequently detected in both the environment and agricultural products. However, its adverse effects on aquatic organisms remain unknown. In this study, the adverse effects of ecologically relevant TFS-TBZ concentrations (i.e., 75.0, 112.5, and 150.0 μg/L) on the heart and development of zebrafish were investigated. TFS-TBZ was found to substantially hinder development, inhibit growth, and cause significant abnormity at higher concentrations. Moreover, TFS-TBZ caused severe pericardial edema, heart loop failure, cardiac linearization, and ultra-slow heartbeat, implying that TFS-TBZ might induce congenital heart disease. TFS-TBZ inhibited Notch signaling and increased the intracellular generation of reactive oxygen species, resulting in decreased myocardial cell proliferation and increased apoptosis. The use of sodium valproate and Gadofullerene illustrated the relevance of the Notch signaling system and oxidative stress. Finally, TFS-TBZ exposure conveys severe developmental toxicity to the zebrafish heart. The underlying mechanism is regulation notch mediated‐oxidative stress generation, implying that TFS-TBZ may be potentially hazardous to aquatic organisms in the environment.
研究领域:
环境毒理学
·
水生生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Jingjing Li,Jia Cheng,Juan Du,Ming Xiao,Miao Wang,Jing Wang,Yongxin She,A.M. Abd El-Aty,Xiaolin Cao
The main challenge in Raman detection of pesticide residues in agricultural products is matrix interference, which hinders accurate identification of trace compounds in complicated agricultural samples. In this study, a novel surface-enhanced Raman spectroscopy approach based on magnetic molecularly imprinted polymers (MMIP-SERS) was introduced for the rapid analysis of myclobutanil and tebuconazole in apples. The synthesized magnetic molecularly imprinted polymer selectively adsorbed triazole pesticides, effectively eliminating matrix interference in real samples. The saturation adsorption time for MMIPs was only 10 min. In addition, the synthesized colloidal gold served as an excellent substrate for enhancing the specific signals for myclobutanil and tebuconazole . We successfully identified the characteristic peak of tebuconazole at 1089 cm −1 , while myclobutanil showed its characteristic peak at 632 cm −1 . The series of experiments and recovery tests confirmed the high sensitivity of the developed MMIP-SERS method using colloidal gold as an enhanced substrate for fungicide analysis in apple samples. The linear detection ranges for myclobutanil and tebuconazole in apples were determined to be 0.2–10 mg/kg and 0.5–10 mg/kg, respectively. The limit of detection was 0.09 mg/kg for tebuconazole and 0.05 mg/kg for myclobutanil. This study provides a rapid and simple method for detecting myclobutanil and tebuconazole based on the synergistic effect of the imprinted polymer and SERS with good separation and detection performance.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 29
·期 1
·163
·
IF 4.6
Siwei Wang,Yanping Liu,Manshan Zhu
The present study describes the development of a highly effective approach for determining the residue distribution and dissipation of trifloxystrobin and tebuconazole, and their risk assessment in brown rice, husk, straw, and grain using high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS). The current study provides considerable novel information regarding the safe utilization of a mixture of trifloxystrobin and tebuconazole in paddy production. The samples demonstrated a range of mean recoveries between 72% and 86%, with a 1.1–9.2% relative standard deviation (RSD). The limits of quantification (LOQ) and half-lives (t1/2) for brown rice, husk, straw, and grain were, respectively, established to be 0.001–0.01 mg/kg and 4.1–7.7 days. The concentrations of terminal residues in the brown rice, husk, straw, and grain were, respectively, found to be 0.02–0.05, 0.03–0.11, 0.02–0.07, and 0.02–0.05 mg/kg after being treated twice at 168.75 g a.i./ha with 21 and 28 days of pre-harvest intervals (PHIs). Trifloxystrobin and tebuconazole presented a non-negligible chronic risk to human subjects, as evidenced by a risk quotient (RQ) value of less than 1.Keywords:trifloxystrobin and tebuconazole;paddy;residue distribution;risk assessment;dissipation dynamics
研究领域:
农业科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2025
·Vol. 48
·期 1
·e70077
·
IF 2.8
Marzieh Kavian,Milad Ghani,Jahan Bakhsh Raoof
Herein, an amino-functionalized covalent organic framework was synthesized and accommodated in the pores of porous hollow fiber. In this context, tetra (4-aminophenyl) porphyrin was synthesized for preparing the desired covalent organic framework as the extracting sorbent and employed for hollow fiber solid-phase microextraction of tebuconazole and propiconazole. With respect to the amino groups of the as-synthesized porphyrin-based covalent organic framework, the extracting device has the ability of establishing a hydrogen bond with the selected model analytes. Under the optimum condition, the linear range of the method for both analytes were estimated in the range of 0.5–200 µg L −1 (the coefficient of determination of 0.9962 for tebuconazole and 0.9990 for propiconazole). The limits of detection of the method for tebuconazole and propiconazole were calculated to 0.02 µg g −1 and 0.03 µg g −1 , respectively. The limits of quantification of the method were also estimated for two analytes equal to 0.07 and 0.08 µg g −1 , respectively. The intra- and inter-day relative standard deviations, which fell between 1.8% and 4.7%, were computed to assess the accuracy of the suggested approach. The proposed method was used for the extraction and determination of tebuconazole and propiconazole in tomato, cucumber, apple, cabbage, and carrot, which the obtained results showed the success of the method in extracting and determining of these analytes from the target samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Jinhua Jiang,Liezhong Chen,Xingang Liu,Luyan Wang,Shengan Wu,Xueping Zhao
The combination effects of triazole fungicides on aquatic organisms remain largely unknown. In current study, an integrated histological, transcriptome, metabonomics and microbiology was applied to investigate the mixture effects and risk of tebuconazole (TEB) and difenoconazole (DIF) co-exposure on zebrafish liver and gonad at aquatic life benchmark. TEB and DIF mixture showed additive effect on the acute toxicity to adult zebrafish, the combined toxicity on liver was less than the additive effect of individual TEB and DIF, and TEB and DIF mixture also reduced the toxic effects on gonad and intestinal microflora. Transcriptomics and metabolomics further showed TEB and DIF mixture could induce more differentially expressed genes (DEGs) to regulate the metabolic pathways involved in energy metabolism, steroid hormone biosynthesis, retinol metabolism and microbial metabolism, to balance the energy metabolism and supplies, and maintain the steroid hormone and RA level, further reduced the toxic effect on liver and gonad caused by TEB and DIF. Our results showed the different responses and patterns on transcriptional and metabolic profiles mediated in the diverse toxicity and combination effects of TEB and DIF. The present results provided a deep mechanistic understanding of the combined effects and mode of action of DIF and TEB mixture on aquatic organisms, suggesting the concept of additive effects might sufficiently protective when evaluated the combination effects and ecological risk of TEB and DIF at aquatic life benchmarks.
研究领域:
生态毒理学
·
分子生物学
·
环境科学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Linbin Zheng,Meng Li,Zhouyu Jiang,Junyan Fan,Zengbo Fang,Jihuai Zheng,Yan Cui
Background The increased production and use of chiral pesticides will enhance their exposure in the environment. Chiral pesticides typically exhibit varied biological effects among these enantiomers. Therefore, it is very essential to develop and validate chiral analytical methods to investigate their potential ecological risks from a stereoselective perspective. Current separation of pesticides enantiomers relies extensively on chiral stationary phases (CSPs), while the development of β-Cyclodextrin derivatives CSPs become the research focus due to their great modifiability and excellent chiral recognition capabilities. Results A novel chiral stationary phase, 3,5-dichlorophenylaminomethyl-6-phenylenediamine-β-cyclodextrin chemically bonded silica gel (MPDCDA), was successfully prepared. Based on that, a stereoselective HPLC-MS/MS method was developed and validated for the determination of tebuconazole enantiomers in Rana nigromaculata tadpoles. After extraction by QuEChERS, the tebuconazole enantiomers were completely separated with the resolutions of 1.63 using the mobile phase of methanol-water (70/30, v/v). Good linearity (r > 0.9990) for both enantiomers over a concentration range of 0.20–500.0 ng/mL was obtained with the accuracy ranged from 6.7 % to 9.3 % and the intra-day and inter-day precisions below 6.2 % at three quality control levels. The proposed method was successfully applied in evaluating the enantioselective bioaccumulation and elimination profiles of tebuconazole in tadpoles. At the tested conditions, there was no significantly enantioselective difference in the bioaccumulation process for S - tebuconazole and R -tebuconazole. However, the elimination process of tebuconazole enantiomers was enantioselective with R -tebuconazole preferentially degraded. Significance This work provided an accurate risk assessment of chiral pesticides to non-target aquatic organisms from a stereoselective perspective. These findings would deepen our understanding of the potential ecological risks of chiral pesticides on aquatic organisms and provide scientific support for the protection of aquatic organisms and their ecological environments, as well as sustainable development.
研究领域:
分析化学
·
环境科学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Chuanyu Han,Zhiyin Yu,Yuting Zhang,Zhiyan Wang,Junwei Zhao,Sheng-Xiong Huang,Zhonghua Ma,Ziyue Wen,Chongxi Liu,Wensheng Xiang
In this study, the supernatant extract from fermentation broth of Streptomyces sp. NEAU-H3 showed strong antifungal activity against Fusarium graminearum strain PH-1 in vitro and in vivo. Three known pyranonaphthoquinones were isolated by means of an activity-guided method, and frenolicin B was characterized as the main active ingredient. Frenolicin B displayed strong antifungal activity against F. graminearum strain PH-1 with an EC50 value of 0.51 mg/L, which is lower than that of carbendazim (0.78 mg/L) but higher than that of phenamacril (0.18 mg/L). Frenolicin B could also strongly inhibit the mycelial growth of Fusarium species, including F. graminearum and F. asiaticum, as well as carbendazim-resistant Fusarium strains isolated from field, with EC50 values of 0.25–0.92 mg/L. Results from field experiments showed that the efficacy of frenolicin B in controlling Fusarium head blight at a treatment concentration of 75 g ai/ha was better than those of phenamacril (375 g ai/ha) and carbendazim (600 g ai/ha) or had no significant difference with that of phenamacril (375 g ai/ha) in 2 years. Scanning electron microscope and transmission electron microscope observations revealed that after treating F. graminearum mycelia with frenolicin B, the mycelia appeared aberrant and had an uneven thickness and swelling, the cytoplasm had disintegrated, and some cell contents were lost. Transcriptome analysis suggests that frenolicin B might inhibit the metabolism of nucleotides and energy by affecting genes involved in phosphorus utilization but did not affect the expression of myosin 5, which is the specific target of phenamacril. These findings indicate that frenolicin B may be a potential agrochemical fungicide for controlling Fusarium head blight.
研究领域:
农业
·
植物病理学
·
微生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Ling Zhang,Yu Long,Long-Fei Li,Xiang-Wei Xin,Xiao-Di Chen,Xiang Zhou,Li-Wei Liu,Zhi-Bing Wu,Song Yang
The overreliance on traditional chemical fungicides, combined with the emergence of resistance, poses significant challenges for food safety. Early blight, caused by the fungal pathogen Alternaria solani ( A. solani ), is among the most significant contributors to pre- and postharvest yield losses in tomato cultivation. To address this challenge, through evaluation of the antifungal activity of 52 commercially available natural products against A. solani , we revealed for the first time that the naturally occurring compound genistein exhibited potent fungicidal activity against A. solani , with an EC 50 value of 6.53 μg/mL, surpassing that of chlorothalonil (EC 50 = 8.35 μg/mL). In vivo assays demonstrated that genistein exhibited strong protective (48.1 %) and therapeutic (42.5 %) efficacy, effectively safeguarding tomatoes from fungal infection. Mechanistic investigations, including transcriptomic analysis, scanning electron microscopy, fluorescence imaging, and measurements of malondialdehyde and ATP levels, collectively indicated that genistein disrupted energy metabolism, leading to reactive oxygen species (ROS) accumulation. This oxidative stress cascade triggered both apoptotic-like responses and membrane lipid peroxidation, ultimately inhibiting the growth of A. solani . Interestingly, genistein showed enhanced anti- A. solani activity when used in combination with either chlorothalonil or tebuconazole. Additionally, genistein displayed low phytotoxicity and favorable drug-likeness properties. Taken together, these findings elucidate the mechanisms underlying genistein's antifungal activity against A. solani , underscoring its promise as a natural antifungal agent for enhancing food safety and improving tomato yield and quality. Furthermore, this study provides valuable insights for the rational design of flavonoid-based antifungal agents.
研究领域:
植物病理学
·
植物源杀菌剂
·
农业科学
·
分子生物学
·
真菌学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Lin Li,Xiao-ting Yuan,Zhi-guo Shi,Lan-ying Xu
Chiral stationary phases (CSPs) have always been research hotspot in enantiomer separation. Currently, most of the CSPs are based on silica platform. In this research, monodisperse, porous glycidyl methacrylate-divinylbenzene copolymer particles (poly(GMA-DVB)) were designed and prepared. Then the GMA was further reacted with ethylenediamine to introduce amino groups onto the polymer, which provide anchoring sites for cellulose derivatives. Herein, Cellulose-tris (3,5-dimethylphenylcarbamate) (CDMPC) was successfully coated onto the polymer microspheres, achieving a stable and successful CSP. The porous structure and the surface moieties of the CSPs were studied in detail. The chromatographic separation was optimized. Hexaconazole,methyl DL-mandelate,benzoin and tebuconazole have been successfully separated on the CSP column, with column efficiency as high as 10,200 plates/m, which is comparable with some silica-based CSPs. The research has indicated that the poly(GMA-DVB) is a promising candidate for constructing CSPs for chiral separation.
研究领域:
分析化学
·
色谱学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）