MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Zhiqin Huang,Qingli Zeng,Dan Li,Laisheng Li
The new type of bis- triazolyl bridged cyclodextrin-based stationary phase (BCDP) was prepared through “Click reaction”. BCDP had high selectivity for triazoles and was used for the simultaneous fast separation and determination of enantiomeric residues (flutriafol, hexaconazole and diniconazole) in fruits and vegetables by high-performance liquid chromatography (HPLC). The QuEChERS method assisted by magnetic nanoparticles was used for sample pretreatment, and then a gradient elution was carried out by using MeOH-H 2 O as the mobile phase on BCDP to achieve rapid resolution of the triazole enantiomers within 25 min. After condition optimization, simultaneous baseline separations of the six enantiomers were achieved with resolutions of 1.54 (flutriafol), 2.27 (hexaconazole) and 1.97 (diniconazole). A new HPLC quantification method of the six enantiomeric residues in apples, tomatoes and cucumbers was established by an internal standard with a photo-diode array (PDA) detector at 210 nm. The results showed that the excellent linear relationships of all triazole enantiomers were observed in the concentration range of 0.5–50 mg L -1 ( R 2 = 0.9986–0.9999). The average recoveries of enantiomers in fruits and vegetables were from 91.95 % to 109.92 % with RSDs of 0.97 %–1.32 % for intraday and 1.30 %–3.03 % for interday. The limits of detection (LODs, S/N = 3) and limits of quantitation (LOQs, S/N = 10) of all enantiomers were less than 0.03 mg kg −1 and 0.10 mg kg −1 , respectively. BCDP had high selectivity for triazole due to its synergistic inclusion with triazoles and advantage in fast and simultaneous separation of multiple triazole enantiomers, which could save analysis time and reduce solvent consumption. BCDP was a new separation material for the scientific evaluation of chiral pesticides in the food safety analysis.
研究领域:
食品安全分析
·
农药残留检测
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Hui Liu,Mengqi Liu,Kuiyuan Chen,Mei Shan,Yongye Li
Penthiopyrad is a widely used chiral fungicide for controlling rust and Rhizoctonia diseases. Development of optically pure monomers is an important strategy to realize amount reduction and increment effects of penthiopyrad, wherein, fertilizers as the co-exiting nutrient supplement may alter the enantioselective residues of penthiopyrad in soil. In our study, influences of urea, phosphate, potash, NPK compound, organic granular, vermicompost and soya bean cake fertilizers on enantioselective persistence of penthiopyrad were fully evaluated. This study demonstrated that R -(−)-penthiopyrad dissipated faster than S -(+)-penthiopyrad during 120 days. High pH, available nitrogen, invertase activities and reduced available phosphorus, dehydrogenase , urease, catalase activities were situated to benefit removing the concentrations of penthiopyrad and weakening enantioselectivity in soil. With respect to the impact of different fertilizers on soil ecological indicators, vermicompost contributed to enhanced pH. Urea and compound fertilizer played an absolute advantage in promoting available nitrogen. All fertilizers didn't go against available phosphorus. Dehydrogenase responded negatively to phosphate, potash and organic fertilizers . Urea increased invertase, besides, it and compound fertilizer both diminished urease activity. The catalase activity was not activated by organic fertilizer. Based on all the findings, soil application of urea and phosphate fertilizers was recommended and considered as a better option to exhibit high efficiency for the dissipation of penthiopyrad. The combined environmental safety estimation can effectively guide the treatment of fertilization soils in line with the nutrition requirements and pollution regulation from penthiopyrad.
研究领域:
农业化学
·
土壤学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Yulong Wang,Junli Xu,Yulou Qiu,Pan Li,Beibei Liu,Lifei Yang,Bogdan Barnych,Bruce D. Hammock,Cunzheng Zhang
A monoclonal antibody (mAb) was raised against tebuconazole (TEB) using a hapten where the p-chloro substituent of the TEB molecule was replaced with a long-chain carboxylic acid. The resulting mAb showed high sensitivity and specificity against TEB characterized by ELISA with a half-maximal inhibitory concentration (IC50) of 0.19 ng mL–1 and with cross-reactivity (CR) values below 0.01% to several analogues of triazole fungicides. On the basis of the mAb produced, a quantum dot beads-based fluorescence immunochromatographic test strip assay (QBs–FITSA) was developed for rapid and sensitive detection of TEB in agricultural product samples. The QBs–FITSA exhibited a linear detection range from 0.02 to 1.25 ng mL–1 with a limit of detection (LOD) of 0.02 ng mL–1. Furthermore, using produced mAb, multiple high-throughput rapid immunoassay formats could be achieved as a convenient monitoring tool for evaluation of human and environmental exposure to TEB.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 7
·1432-1439
·
IF 2.516
Ruiyu Liang,Yi Peng,Yuling Hu,Gongke Li
A novel hybrid triazine-imine core-shell magnetic covalent organic polymer with high adsorption capacity and excellent stability was fabricated by surface-assisted in situ growth technique. The composite possesses porous and extended π-conjugated system, and was applied as the magnetic sorbent for efficient enrichment and rapid separation of pesticides. A new analytical method for simultaneous determination of eight pesticides in fruit samples was developed by magnetic solid phase extraction combined with ultra high performance liquid chromatography and tandem mass spectrometry. The effect of extraction time, desorption time, and the type of desorption solvent on the extraction efficiency were evaluated. The established method shows good repeatability and high sensitivity. The repeatability of this method was estimated with relative standard deviations in the range of 0.7–7.0% ( n = 5) for the same batch, and 1.7–10% ( n = 3) for batch to batch. Good linearity for eight pesticides was obtained with coefficient of determination in the range of 0.9942–0.9990. Limit of detections ranged from 0.4 to 1.2 ng/L. Real sample determination showed that four and two pesticides were detected in strawberry and grape, respectively. The results demonstrated that the established method was efficient, sensitive, and convenient for trace determination of pesticides in fruit samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY IN VITRO
·
2023
·
IF 3.2
Jing Nie,Jiabei Zhou,Yi Shen,Ruimiao Lin,Haihong Hu,Kui Zeng,Huichang Bi,Min Huang,Lushan Yu,Su Zeng,Jing Miao
The widespread use of triazole fungicides in agricultural production poses a potential risk to human health. This study investigates the interaction of five triazole fungicides, i.e., tebuconazole, triticonazole, hexaconazole, penconazole, and uniconazole with human renal transporters, including OAT1, OAT3, OCT2, OCTN1, OCTN2, MATE1, MATE2-K, MRP2, MDR1, and BCRP, using transgenic cell models. For uptake transporters, triticonazole was the substrate of OAT1 and OAT3 and the inhibitor of OCT2. Tebuconazole and penconazole inhibited OCTN2 (100 μM), while tebuconazole, triticonazole, hexaconazole, penconazole, and uniconazole inhibited MATE1 (100 μM). Tebuconazole and hexaconazole inhibited MATE2-K (100 μM). All five triazole fungicides were not substrates or strong inhibitors of MRP2, MDR1, and BCRP efflux transporters. Penconazole inhibited OCT2 with IC 50 = 1.12 μM. Penconazole and uniconazole inhibited MATE1 with IC 50 = 0.94 μM and 0.87 μM. Tebuconazole and hexaconazole inhibited MATE2-K with IC 50 = 0.96 μM and 1.04 μM, indicating that triazole fungicides may inhibit renal drug transporter activity at low concentrations. Triticonazole was transported by OAT1 and OAT3, and the K m values of triticonazole were 5.81 ± 1.75 and 47.35 ± 14.27, respectively. Tebuconazole and uniconazole were transported by OAT3, and the K m values of tebuconazole and uniconazole were 30.28 ± 7.18 and 87.61 ± 31.70, respectively, which may induce nephrotoxicity.
研究领域:
药理学
·
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2023
·Vol. 7
·期 5
·955-963
·
IF 7
Yang Liu,Liqiang Liu,Xinxin Xu,Chuanlai Xu,Liguang Xu
As a commonly used crop fungicide with proven adverse effects against various organisms, the excessive use of flutriafol (FTF) has resulted in accumulation of a large amount of residues in crops. In this study, a novel hapten was designed by introducing a long interval arm on its benzene ring allowing sufficient exposure of the FTF active site, for the production of a highly sensitive and specific monoclonal antibody with a half-inhibitory concentration of 1.77 ng mL−1. Furthermore, for the first time, a gold nanoparticle-based lateral flow immunoassay (LFIA) was developed for the quantitative detection of FTF in foods with a visual limit of detection in rice, tomatoes, and grapes of 0.5 mg kg−1, 0.2 mg kg−1 and 0.5 mg kg−1, respectively, and a cutoff value of 4 mg kg−1, and a calculated limit of detection of 0.045 mg kg−1, 0.035 mg kg−1 and 0.060 mg kg−1, respectively. The recoveries in spiked samples ranged from 90.2% to 110.1% and these results indicated that the gold nanoparticle-based LFIA developed in this study for the rapid detection of FTF in food is a reliable on-site detection method.
研究领域:
农业化学
·
食品科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）