Journal of Biophotonics
·
2018
·Vol. 12
·期 2
·e201800134
·
IF 3.768
Jianyi Xu,Yilin Ma,Tingting Yu,Dan Zhu
Various tissue optical clearing techniques have sprung up for large volume imaging. However, there are few methods showed clearing and imaging data on different organs while most of them were focused on mouse brain, and as a result, it is difficult to select the suitable method for organs in practical applications due to lack of quantitative evaluation and comprehensive comparison. Therefore, it is necessary to evaluate and compare the performances of clearing methods for different organs. In this paper, several typical optical clearing methods were applied, including 3DISCO, uDISCO, SeeDB, FRUIT, CUBIC, Sca l eS and PACT to clear intact brain, heart, kidney, liver, spleen, stomach, lung, small intestine, skin and muscle. The clearing efficiency, sample deformation, fluorescence preservation and imaging depth of these methods were quantitatively evaluated. Finally, based on the systemic evaluation of various parameters described above, the appropriate clearing method for specific organ including kidney or intestine was screened out. This paper will provide important references for selection of appropriate clearing methods in related researches.
研究领域:
生物医学工程
·
显微成像
·
组织学
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产品关联
DOI
PubMed
高影响因子
Coatings
·
2022
·Vol. 12
·期 5
·636
·
IF 3.236
Le Wei,Yue Ma,Zhimou Guo,Junjie Ding,Gaowa Jin,An Gu,Yong Lei
Multilayer objects with different interfaces are quite typical for architectural heritage, and from them may be inferred the age, production process, and deterioration mechanism through analyzing characteristic compositions with advanced analytical techniques. The Meiwu ceiling in the Hall of Mental Cultivation of the Palace Museum was found to contain many paper-based layers during conservation. Once several surface strata were detached, a colorful layer of printed fabric textile was discovered integrally. Through microscopic observation and micro-attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) imaging, the overall structure consisted of 11 layers, namely, bast paper, cotton wiring, xuan paper, cotton printed fabric, two yellow board papers, bamboo paper, three wood pulp paper and surface coatings, and starch was considered as an organic adhesive. For identification of the printed fabric’s color palette, ultra-performance liquid chromatography (UPLC) combined with high-resolution quadrupole time-of-flight (QTOF) technology, non-invasive macro X-ray fluorescence (MA-XRF) and desorption electrospray ionization mass spectrometry imaging (DESI-MSI) were applied in situ. Seven industrial synthetic dyes, including auramine O, malachite green, and eosin Y with corresponding by-products, as well as chromium-based pigments considered as dark draft line, were confirmed. By X-ray diffraction (XRD), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS), and micro Fourier transform infrared spectroscopy (micro FTIR, other results showed chalk soil and talc for the outermost coating. According to the synthetic time of industrial dyes and degradation degree of paper, there were at least four occurrences and their specific time periods were speculated.Keywords:organic cultural heritage;ceiling;industrial dyes;imaging;by-products;degradation
研究领域:
文物保护
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2019
·Vol. 103
·期 2
·267-273
·
IF 1.65
Wang Chengcheng,Zheng Shenghui,Zou Xiaoyan,Sun Xia,Zhang Hongwu
The development of nanotechnology has drawn increased attention to the risks of nanoparticles (NPs). In this work, the near-infrared persistent luminescence imaging technique was used to track the biodistribution of NPs in vivo in zebrafish. Zebrafish were used as a vertebrate animal model to show NPs distribution and the effects of exposure. ZnGa 2 O 4 :Cr (ZGOC) was chosen as the probe in this work. In continuous exposure experiments, the results showed more particles accumulated in the intestines than in the gills in both groups. In both the gills and abdomen, the NPs contents were greater in the ZGOC–NH 2 -treated groups than in the ZGOC groups, and the NPs caused damage to the gills and intestines. Removal exposure experiments indicated that ZGOC and ZGOC–NH 2 could be excreted from the body. The metabolism, excretion of NPs, the quantification and monitoring of NPs behavior in biological systems should be examined in further studies.
研究领域:
环境科学
·
毒理学
·
纳米生物学
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产品关联
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2025
·Vol. 13
·期 3
·e70022
·
IF 3.8
Cheng Wu,Yingjie Feng,Jiarui Cui,Zhang Yao,Hailong Xu,Songlei Wang
It is an important measure to ensure food quality and safety that real-time monitoring of the key quality indicators of fresh meat after packaging in the process of storage and transportation. The feasibility of combining hyperspectral imaging (HSI) technology with chemometrics and deep learning to detect the quality deterioration of polyethylene (PE)-packaged raw beef, especially for a key lipid oxidation indicator of malondialdehyde (MDA) content, was explored in this study. The feasibility of filtering to overcome the interference of packaging film on the spectral data was further investigated. Near-infrared HSI (400–1000 nm) was used to collect spectral and spatial data of beef samples during short-term storage. A least squares regression (PLSR) and echo-neural network optimized by vulture optimization algorithms (BES-ESN) models were developed by multivariate data processing methods. The results showed that the performance of models established by PE-packed beef samples was usually inferior to that established by unpacked beef samples. The changes of MDA content in beef were visualized according to the optimal model. In addition, Gaussian filtering was applied to reduce the interference effect caused by the packaging film. The results have demonstrated that HSI combined with Gaussian filter preprocessing and multivariate data processing provided an efficient and reliable tool for detecting the freshness of beef in PE packaging. The best model had a coefficient of determination ( R 2 P ) of 0.8309 and a root mean squared error of prediction (RMSEP) of 0.2180, demonstrating the potential of hyperspectral techniques for real-time monitoring of packaged raw meat quality. The findings can provide some references for the meat industry to develop advanced non-invasive quality assurance systems in the meat industry.
研究领域:
食品科学
·
生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2016
·
IF 2.857
Jing-Bo Liu,Yu-Xin Li,Xiu-Lan Zhang,Xue-Wen Hua,Chang-Chun Wu,Wei Wei,Ying-Ying Wan,Dan-Dan Cheng,Li-Xia Xiong,Na Yang,Hai-Bin Song,Zheng-Ming Li
To discover potent insecticides targeting ryanodine receptors (RyRs), a series of novel anthranilic diamides analogues (12a–12u) containing N-substituted phenylpyrazole were designed and synthesized. These compounds were characterized by 1H NMR, 13C NMR, and HRMS, and the structure of compound 12u was confirmed by X-ray diffraction. Their insecticidal activities indicated that these compounds displayed moderate to excellent activities. In particular, 12i showed 100 and 37% larvicidal activities against oriental armyworm (Mythimna separata) at 0.25 and 0.05 mg L–1, equivalent to that of chlorantraniliprole (100%, 0.25 mg L–1; and 33%, 0.05 mg L–1). The activity of 12i against diamondback moth (Plutella xylostella) was 95% at 0.05 mg L–1, whereas the control was 100% at 0.05 mg L–1. The calcium-imaging technique experiment results showed that the effects of 12i on the intracellular calcium ion concentration ([Ca2+]i) in neurons were concentration-dependent. After the central neurons of Helicoverpa armigera were dyed by loading with fluo-5N and treated with 12i, the free calcium released in endoplasmic reticulum indicated the target of compound 12i is RyRs or IP3Rs. The activation of RyRs by natural ryanodine completely blocked the calcium release induced by 12i, which indicated that RyRs in the central neurons of H. armigera third-instar larvae is the possible target of compound 12i.
研究领域:
农药化学
·
昆虫生理学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Yanbo Liu,Xiaolei Wang,Chenyang Zhao,Shuo Wang,Xiaogang Lian,Weiqi Chen,Lei He,Mingyang Chen,Songgu Wu,Junbo Gong
Tenofovir amibufenamide fumarate (TMF) is the first oral drug developed in Asia for the treatment of adult patients with chronic viral hepatitis B, however, further applications are limited by poor tableting performance and high sticking propensity. In this work, the spherulitic growth process of TMF has been designed and explored with the help of molecular dynamics simulation and process analysis technologies (ATR-FTIR, FBRM and EasyViewer). The spherical particles with high bulk density, good flowability and uniform particle size distribution are prepared by a simple quenching process. More importantly, experimental results show that spherical particles have higher average tensile strength (100.8% increase), higher plastic deformability and lower amount of punch sticking (87.4% decrease in 30 tablets) compared to the commercial powder products. These contributions not only shed light on the design principle of drug spherulitic growth processes, but also provide guidance for the manufacture of high-quality tablet products.
研究领域:
药学
·
结晶工程
·
材料科学
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2017
·Vol. 243
·期 12
·2265-2273
·
IF 1.664
Wang Xiaobin,Huang Wenqian,Zhao Chunjiang,Wang Qingyan,Liu Chen,Yang Guiyan
Raman hyperspectral imaging technology not only can acquire the image information of the sample; it also contains the Raman spectra information about each pixel. Due to the abundant information that the method provides, it has been applied to detect food safety. This study adopted line-scan Raman hyperspectral technology to quantify benzoyl peroxide (BPO) additive in flour. By analyzing the Raman spectra of BPO and flour, the 999 cm −1 Raman peak was selected for the detection and identification of BPO in flour. Savitzky–Golay filter and adaptive iteratively reweighted penalized least squares (airPLS) methods were used to de-noise and fluorescence correction of the original Raman signals. Binary image was established by 999 cm −1 single-band correction image and threshold segmentation, and this method was used to detect 11 mixture samples with different BPO additive concentrations. The results show that the BPO additives in the mixture samples can be detected, and the detected BPO pixels had a good linear relationship with the concentration of BPO in the mixture samples, correlation coefficient was 0.9902. The above results indicated that the method established in this paper can be applied to non-destructive quantitative detection of BPO additive in flour.
研究领域:
食品科学
·
分析化学
·
光谱学
✓ 已验证
DOI
高影响因子
Nano Research
·
2023
·
IF 9.9
Cao Rong,Tang Ning,Zhu Yi,Chen An,Li Yumeng,Ge Renbin,Li Yuan,Huang Zhongyi,Guo Jiajing,Deng Jiali,Lu Hongwei,Lu Ziwei,Forgham Helen,Davis Thomas P.,Qiao Ruirui,Wang Zhongling
The development of microenvironment-responsive nanoprobes has shown great promise for use in magnetic resonance imaging (MRI), with the advantage of significantly improved specificity and good biocompatibility. However, the clinical application of responsive probes is hampered by a lack of biological sensitivity for early molecular diagnostics and visualizing microenvionment of metabolism reprogramming in tumor progression. Here, we report on a dual-ratiometric magnetic resonance tunable (DMRT) nanoprobe designed by crosslinking different ratios of transferrin chelating gadolinium and superparamagnetic nanoparticles, complexed to a pH responsive biocompatible polymer. This dually activatable nanoprobe enables pH-dependent tumor microenvironment visualization, providing exceptional quantitative pathophysiological information in vitro and in vivo . When used in combination with dual-contrast enhancement triple subtraction imaging technique (DETSI), this smart nanoprobe guarantees the diagnosis of early-stage diseases. We envisage that this novel integrated nanoplatform will provide a new paradigm for the clinical translation of robust DMRT nanoprobes for early disease detection and staging, as well as microenvironment visualization and disease progression monitoring.
研究领域:
生物医学工程
·
纳米技术
·
医学成像
·
癌症研究
·
分子诊断
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2024
·
IF 5.7
Dongdong Liang,Zhiheng Xu,Dandan Yang,Zhibin Xu,Weitong Yin,Xiaobin Tang
Luminescence instability in service environments has limited the widespread use of halide perovskite materials, especially for X-ray energy conversion and detection/imaging. This work proposed a method for improving luminescence stability through polymer encapsulation, which is more convenient than compositional engineering and surface modulation. Meanwhile, the differences in the luminescence intensities of composite films synthesized by encapsulation of different polymer matrices and their luminescence stability under various harsh X-ray irradiation conditions were systematically investigated. The applications of the developed CsPbBr3-embedded polymer films with excellent luminescence performance and high service stability in X-ray radiation energy utilization and detection/imaging were discussed. The results show that CsPbBr3-embedded PS films can meet the spectral modulation requirements of radioluminescent materials for nuclear batteries and can also fulfill the needs of flexible X-ray imaging with a high spatial resolution.
研究领域:
材料科学
·
辐射物理
·
核工程
·
成像技术
✓ 已验证
产品关联
DOI
高影响因子
APL Photonics
·
2024
·Vol. 9
·期 7
·
IF 5.4
Li Xi,Yang Tianhao,Zhang Zhongyang,Wu Shengnan,Yuan Zhen,Zhou Feifan
The cerebral lymphatic drainage plays an important role in the occurrence and development of central nervous system diseases. Recent studies have shown that cerebral lymphatic drainage is regulated by circadian rhythm and anesthesia state; however, the regulating mechanism is still unclear. In this study, we used the second near-infrared region in vivo imaging to explore the regulation of cerebral lymphatic drainage in mice at different states. At first, by injection of a tracer at different times, we confirmed that the drainage of the meningeal lymphatic system was the fastest at zeitgeber time 2, while the internal flow of the glymphatic system was the slowest. Under anesthesia with isoflurane, administration of dexmedetomidine, an anesthetic that inhibits norepinephrine (NE) release, enabled mice to enter the stage of non-rapid eye movement sleep, at which time the influx of the glymphatic system increased, the efflux of the meningeal lymphatic system decreased, and the clearance rate of the brain parenchyma decreased. However, following the exogenous NE supplement, mice quickly changed from a non-rapid eye movement stage into an awake state with the meningeal lymphatic drainage retrieval. The results showed whether the drainage of the glymphatic system and meningeal lymphatic vessels, or parenchymal clearance, has made rapid adjustments based on sleep status that is regulated by NE. This study reveals that the NE-regulated sleep–wake cycle is a powerful regulator of cerebral lymphatic drainage and provides a potential therapeutic target for related central nervous system diseases.
研究领域:
神经科学
·
成像技术
·
药理学
·
生理学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chenxing Xu,Nan Wang,Tao Ma,Shengrong Pei,Meiting Wang,Jinpeng Yu,Dongting Zhangsun,Xiaopeng Zhu,Sulan Luo
α 3 β 4, a vital subtype of neuronal nicotinic acetylcholine receptors (nAChRs), is widely distributed in the brain, ganglia, and adrenal glands, associated with addiction and neurological diseases. However, the lack of specific imaging tools for α 3 β 4 nAChRs has hindered the investigation of their tissue distribution and functions. [D11A]LvIA, a peptide derived from marine cone snails, demonstrates high affinity and potency for α 3 β 4 nAChRs, making it a valuable pharmacological tool for studying this receptor subtype. In this study, three fluorescent conjugates of [D11A]LvIA were synthesized using 6-TAMRA-SE (R), Cy3-NHS-ester (Cy3), and BODIPY-FL NHS ester (BDP) dyes. The electrophysiological activities were assessed in Xenopus laevis oocytes by two-electrodes voltage clamp (TEVC). [D11A]LvIA-Cy3 and [D11A]LvIA-BDP show improved selectivity and affinity, with IC 50 values of 512.70 nM and 343.50 nM, respectively, and [D11A]LvIA-Cy3 exhibits better stability in cerebrospinal fluid. Utilizing [D11A]LvIA-Cy3, we successfully visualized the distribution of α 3 β 4 nAChRs in rat trigeminal ganglia, retina, adrenal glands, and various brain regions. This novel fluorescent peptide provides a significant pharmacological tool for the exploration and visualization in-situ distribution of α 3 β 4 nAChRs in different tissues and also assists in clarifying the function.
研究领域:
神经科学
·
药理学
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Light-Science & Applications
·
2024
·Vol. 13
·期 1
·1-11
·
IF 20.6
Dong Siyin,Fan Zhenghui,Wei Wei,Tie Shujie,Yuan Ruihan,Zhou Bin,Yang Ning,Zheng Xiaojia,Shen Liang
Quasi-two-dimensional (Q-2D) perovskite exhibits exceptional photoelectric properties and demonstrates reduced ion migration compared to 3D perovskite, making it a promising material for the fabrication of highly sensitive and stable X-ray detectors. However, achieving high-quality perovskite films with sufficient thickness for efficient X-ray absorption remains challenging. Herein, we present a novel approach to regulate the growth of Q-2D perovskite crystals in a mixed atmosphere comprising methylamine (CH 3 NH 2 , MA) and ammonia (NH 3 ), resulting in the successful fabrication of high-quality films with a thickness of hundreds of micrometers. Subsequently, we build a heterojunction X-ray detector by incorporating the perovskite layer with titanium dioxide (TiO 2 ). The precise regulation of perovskite crystal growth and the meticulous design of the device structure synergistically enhance the resistivity and carrier transport properties of the X-ray detector, resulting in an ultrahigh sensitivity (29721.4 μC Gy air −1 cm −2 ) for low-dimensional perovskite X-ray detectors and a low detection limit of 20.9 nGy air s −1 . We have further demonstrated a flat panel X-ray imager (FPXI) showing a high spatial resolution of 3.6 lp mm −1 and outstanding X-ray imaging capability under low X-ray doses. This work presents an effective methodology for achieving high-performance Q-2D perovskite FPXIs that holds great promise for various applications in imaging technology.
研究领域:
材料科学
·
物理学
·
电气工程
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）