BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Jingyao Gong,Yunlong Li,Hui Di,Jiamin Li,Junming Dong,Congjie He,Peng Cao,Huiming Cai,Jian He,Yiqing Wang
Combination of PET and optical imaging techniques holds significant potential for complete tumor resection to optimize patient outcomes. In this study, we combined two lapatinib-based molecular probes for detecting HER2-overexoression tumors through PET and near-infrared (NIR) fluorescence imaging. HER2 receptor had been confirmed to be overexpressed in many types of tumors and had become a specific target for molecular imaging. The radiolabeled probe [ 68 Ga] Ga-NOTA-lapatinib demonstrated superior imaging performance in micro-PET scans, achieving clear differentiation of subcutaneous tumors from adjacent tissues. Complementarily, the NIR fluorescent probe LP-S exhibited prolonged tumor retention (>72 h) with distinct signal demarcation between malignant and normal tissues in ex vivo analyses. Fluorescence microscopy further validated specific intra-tumoral accumulation of the probe at cellular resolution. This synchronized dual-probe strategy, leveraging identical targeting moieties, established a robust platform for multimodality cancer imaging that bridges preoperative detection with intraoperative visualization.
研究领域:
医学影像学
·
分子生物学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-11
·
IF 6.232
Zheng Xiaoke,Li Chenghui,He Yaqin,Wu Lan,Hou Xiandeng
In this work, a novel bi-modal imaging probe with enhanced CT contrast efficiency and FL brightness was constructed, in which the combination of a binary CT contrast agent BaHoF 5 and Cu-doped QDs served as a vehicle; hyaluronic acid (HA) was employed as a tumor-targeting ligand. With its CT contrast efficiency about 2.1- and 3.9-fold higher than PEG-BaHoF 5 and Iohexol, the CT contrast efficiency and the fluorescent brightness of the bi-modal probe were both enhanced. Likewise, its fluorescent brightness is almost 6-fold brighter after Cu-doped QDs loading. The most important contribution of this work lies on the proposed strategy. The inherent contradiction of the imaging sensitivity of CT and FL imaging is well balanced and a great CT/FL bi-modal imaging performance is simultaneously obtained even at low concentration (400 μg/mL) of the probe, which was superior to the previous CT/FL bi-modal probes. Moreover, since BaHoF 5 as a binary CT contrast agent was introduced instead of conventional Au and Bi 2 S 3 , the CT/FL bi-modal probe would be more suitable for different patients under different operation voltages. In addition, the in vitro tumor cell imaging also demonstrated a good photo-stability, FL brightness, and tumor-targeting capability of the probe, indicating its great potential in practical bi-modal imaging for further tumor diagnosis and therapy.
A novel bi-modal imaging probe with enhanced CT contrast efficiency and FL brightness was fabricated, in which its CT contrast efficiency was about 2.1- and 3.9-fold higher than PEG-BaHoF 5 and Iohexol, respectively, and its fluorescent brightness almost 6-fold brighter after Cu-doped QDs loading.
研究领域:
医学影像学
·
纳米医学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 1
·92-101
·
IF 4.872
Yi Pan,Jun Yang,Yaning Fang,Junhui Zheng,Rong Song,Changqing Yi
In this study, an efficient and mild approach is reported for the facile synthesis of a carbon quantum dot (CQD)-based dual-modal fluorescence (FL)/magnetic resonance (MR) imaging probe by doping Gd(III) into CQDs through a one-pot pyrolysis process at low temperature. The as-prepared Gd-encapsulated CQDs (GCDs), which have an average diameter of ∼15 nm, are highly water-soluble. The GCDs are observed to have a high MR response with a longitudinal relaxation of 57.42 mM−1 s−1 and a strong fluorescence brightness with an absolute quantum yield of 40% while containing only 1.0% (w/w) of Gd3+ content. Stemming from the minimum Gd-doping and the inert carbon coating, GCDs exhibit excellent biocompatibility and blood compatibility. Dual-modality bioimaging applications of GCDs are successfully demonstrated by the use of HeLa cells and mice as models, revealing their great potential in fundamental biomedical research studies and even clinical fields such as fluorescence-guided surgery and dual-modal FL/MR imaging of blood vessels.
研究领域:
生物医学工程
·
材料科学
·
医学影像学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Chunli Jiang,Zhitao Shen,Chunhua Luo,Hechun Lin,Rong Huang,Yiting Wang,Hui Peng
A facile one-pot strategy has been developed for the aqueous synthesis of Gd doped CdTe (Gd:CdTe) QDs as fluorescence and magnetic resonance imaging dual-modal agent. The prepared Gd:CdTe QDs showed narrow size distribution and the average size was less than 5 nm. The amount of Gd 3+ dopant in Gd:CdTe QDs significantly affected the optical properties of obtained QDs. The highest PL QY for the prepared Gd:CdTe QDs was up to 42.5%. The QDs showed the weak toxicity and significant enhancement in MRI signal. The specific relaxivity value ( r1 ) was determined to be 4.22 mM −1 s −1 . These properties make the prepared Gd:CdTe QDs be an effective dual-modal imaging agent and have great potential applications in biomedical field.
研究领域:
材料科学
·
医学影像
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2013
·
IF 7.604
Zhen Liu,Enguo Ju,Jianhua Liu,Yingda Du,Zhengqiang Li,Qinghai Yuan,Jinsong Ren,Xiaogang Qu
Nanoparticulate contrast agents have attracted a great deal of attention along with the rapid development of modern medicine. Here, a binary contrast agent based on PAA modified BaYbF 5 :Tm nanoparticles for direct visualization of gastrointestinal (GI) tract has been designed and developed via a one-pot solvothermal route. By taking advantages of excellent colloidal stability, low cytotoxicity, and neglectable hemolysis of these well-designed nanoparticles , their feasibility as a multi-modal contrast agent for GI tract was intensively investigated. Significant enhancement of contrast efficacy relative to clinical barium meal and iodine-based contrast agent was evaluated via X-ray imaging and CT imaging in vivo. By doping Tm 3+ ions into these nanoprobes , in vivo NIR–NIR imaging was then demonstrated. Unlike some invasive imaging modalities , non-invasive imaging strategy including X-ray imaging, CT imaging, and UCL imaging for GI tract could extremely reduce the painlessness to patients, effectively facilitate imaging procedure, as well as rationality economize diagnostic time. Critical to clinical applications, long-term toxicity of our contrast agent was additionally investigated in detail, indicating their overall safety. Based on our results, PAA-BaYbF 5 :Tm nanoparticles were the excellent multi-modal contrast agent to integrate X-ray imaging, CT imaging, and UCL imaging for direct visualization of GI tract with low systemic toxicity.
研究领域:
医学影像学
·
纳米医学
·
生物材料学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Jinglei Du,Shizhao Zhou,Yihua Ma,Yingying Wei,Qiang Li,Hui Huang,Lin Chen,Yongzhen Yang,Shiping Yu
Gadolinium-doped carbon dots (Gd-CDs), as a new class of nanomaterial, has a wide application prospect in targeted imaging and monitoring diagnosis and treatment of liver cancer because of their good fluorescence (FL)-magnetic resonance (MR) imaging properties. First, Gd-CDs were synthesized by hydrothermal method with gadodiamide as gadolinium source, citric acid as carbon source and silane coupling agent (KH-792) as coupling agent with FL quantum yield (QY) of 48.2%. Then, folic acid (FA), which is highly expressed in liver cancer, was used as a targeting component to modify Gd-CDs to obtain targeted imaging agent (Gd-CDs-FA). The results showed that Gd-CDs and Gd-CDs-FA have low cytotoxicity and good biocompatibility, and the targeting and selectivity of Gd-CDs-FA to HepG 2 cells could be observed under confocal laser scanning microscope (CLSM). The T1 longitudinal relaxation rates (r1) of Gd-CDs and Gd-CDs-FA are 15.92 mM -1 s -1 and 13.56 mM -1 s -1 , respectively. They showed good MR imaging ability in vitro and in vivo, and MR imaging in nude mice further proved the targeting imaging performance of Gd-CDs-FA. Therefore, Gd-CDs-FA with higher QY showed good FL-MR targeting imaging ability of liver cancer, which broke through the limitations of single molecular imaging probe in sensitivity and soft tissue resolution. This study provides a new idea for the application of Gd-CDs in FL and MR targeting imaging of liver cancer.
研究领域:
纳米技术
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医学成像
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癌症研究
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 19
·3383
·
IF 5.719
Dongya Wang,Jingcan Qin,Chuan Zhang,Yuehua Li
Black phosphorus nanomaterials (BPN) have been well developed in tumor therapy. However, lack of diagnostic function limits the development of BPN in biomedicine. Lanthanide-doped nanoparticles are considered as versatile materials for fluorescence or magnetic resonance imaging. Integration of BPN with lanthanide-doped nanoparticles was rarely reported owing to the complex synthesis processes and poor modification effect. Herein, we report a simple and general method for synthesizing BPN@NaReF4(Re: Gd or Y, Yb, Er) nanocomposite. TEM and XRD characterization confirm efficient combination of BPN and NaGdF4or NaYF4:Yb,Er (18.2 mol %) after one-step mixing. The FTIR and XPS spectra were used to prove the generation of PO43−-Gd and P-Gd coordination bonds and clarify ligand exchange mechanism. The anchored nanoparticles on BPN were stable and become hydrophilic. The prepared BPN@NaGdF4exhibit the signals of photoacoustic and magnetic resonance imaging. The obtained BPN@NaYF4:Yb,Er (18.2 mol %) have the potential in fluorescence bioimaging. We believe that this work will expand the applications of BPN in diagnosis and therapy together.
研究领域:
材料科学
·
生物医学工程
·
纳米医学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2017
·Vol. 28
·期 5
·1-11
·
IF 2.325
Zhu Wei,Liang Shuang,Wang Jing,Yang Zhe,Zhang Li,Yuan Tianmeng,Xu Zushun,Xu Haibo,Li Penghui
Abstract Multifunctional nanocomposites based on BaGdF 5 nanoparticles (NPs) and metal phenolic network (MPN) have been engineered as novel contrast agents for potential applications in X-ray computed tomography, magnetic resonance and luminescence imaging. The BaGdF 5 @MPN nanocomposites were synthesized at room temperature by coating BaGdF 5 NPs with europium-phenolic network, which was obtained by the coordination of europium (III) with tannic acid (TA). The in vitro cytotoxicity assays against HepG2 cells revealed that the BaGdF 5 @MPN nanocomposites presented better cytocompatibility and lower cytotoxity than pure BaGdF 5 NPs. In addition, vivid red and green luminescence can be observed by confocal laser scanning microscope (CLSM) from the BaGdF 5 @MPN nanocomposites laden HepG2 cells under the excitation of UV (390 nm) and visible light (440 nm), respectively. The longitudinal relaxivity value (r 1 ) of the nanocomposites was 2.457 mM −1 s −1 . Moreover, the nanocomoposites exhibited X-ray computed tomography (CT) and T 1 -weighted magnetic resonance (MR) imaging capacities, and the intensities of the enhanced signals of in vitro CT and MR images were proportional to the concentrations of the nanocomposites. These results indicated that the as-prepared BaGdF 5 @MPN nanocomposites are promising contrast agents for CT/MR/luminescence imaging. Graphical Abstract
研究领域:
医学影像学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ULTRASONICS
·
2015
·
IF 1.942
Weibao Qiu,Congzhi Wang,Yongchuan Li,Juan Zhou,Ge Yang,Yang Xiao,Ge Feng,Qiaofeng Jin,Peitian Mu,Ming Qian,Hairong Zheng
Ultrasound elastography is widely used for the non-invasive measurement of tissue elasticity properties. Shear wave imaging (SWI) is a quantitative method for assessing tissue stiffness. SWI has been demonstrated to be less operator dependent than quasi-static elastography, and has the ability to acquire quantitative elasticity information in contrast with acoustic radiation force impulse (ARFI) imaging. However, traditional SWI implementations cannot acquire two dimensional (2D) quantitative images of the tissue elasticity distribution. This study proposes and evaluates a scanning-mode 2D SWI (s2D-SWI) system. The hardware and image processing algorithms are presented in detail. Programmable devices are used to support flexible control of the system and the image processing algorithms. An analytic signal based cross-correlation method and a Radon transformation based shear wave speed determination method are proposed, which can be implemented using parallel computation. Imaging of tissue mimicking phantoms, and in vitro , and in vivo imaging test are conducted to demonstrate the performance of the proposed system. The s2D-SWI system represents a new choice for the quantitative mapping of tissue elasticity, and has great potential for implementation in commercial ultrasound scanners.
研究领域:
医学影像学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2014
·
IF 4.384
Jun Liu,Xiumei Tian,Ningqi Luo,Chuan Yang,Jun Xiao,Yuanzhi Shao,Xiaoming Chen,Guowei Yang,Dihu Chen,Li Li
Monoclinic Gd2O3:Eu3+ nanoparticles (NPs) possess favorable magnetic and optical properties for biomedical application. However, how to obtain small enough NPs still remains a challenge. Here we combined the standard solid-state reaction with the laser ablation in liquids (LAL) technique to fabricate sub-10 nm monoclinic Gd2O3:Eu3+ NPs and explained their formation mechanism. The obtained Gd2O3:Eu3+ NPs exhibit bright red fluorescence emission and can be successfully used as fluorescence probe for cells imaging. In vitro and in vivo magnetic resonance imaging (MRI) studies show that the product can also serve as MRI good contrast agent. Then, we systematically investigated the nanotoxicity including cell viability, apoptosis in vitro, as well as the immunotoxicity and pharmacokinetics assays in vivo. This investigation provides a platform for the fabrication of ultrafine monoclinic Gd2O3:Eu3+ NPs and evaluation of their efficiency and safety in preclinical application.
研究领域:
生物医学工程
·
材料科学
·
医学影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORINE CHEMISTRY
·
2017
·
IF 2.101
Songxia Yu,Zhiqiang Wang,Ruijun Cao,Lingjie Meng
In this paper, we have developed a series of rare earth fluoride nanocrystals (NCs) that can be used for multimodal imaging applications. Three highly monodisperse and water dispersible NaGdF 4 :Yb,Ln@NaGdF 4 (Ln = Er, Tm, Ho) upconversion luminescence (UCL) NCs with an average size of 75 nm were prepared using a facile and efficient microwave–assisted solvothermal reaction in ethylene glycol (EG). The morphology, crystal structures, size distributions and zeta potentials of the resulting NCs were fully characterized by transmission electron microscopy (TEM), X–ray diffraction (XRD) and Nano–ZS90 Zetasizer. The obtained NaGdF 4 :Yb,Ln@NaGdF 4 (Ln = Er, Tm, Ho) NCs could emit bright blue, green and red luminescence in water upon laser excitation at 980 nm. Furthermore, these core–shell NCs exhibited low cytotoxicity for HeLa and C9H2 cells, separately. Moreover, these NCs also displayed excellent Hounsfield units (HU) values in X–ray computed tomography (CT) imaging and high relaxivity parameters (r 1 ) in magnetic resonance (MR) imaging in vitro . Thus, these small, water–dispersible and biocompatible NCs show excellent potential as contrast agents for UCL/CT/MR multimodal imaging and other biomedical applications.
研究领域:
材料科学
·
生物医学工程
·
影像医学
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2015
·Vol. 17
·期 12
·1-11
·
IF 2.184
Song Ji-Tao,Yang Xiao-Quan,Zhang Xiao-Shuai,Yan Dong-Mei,Yao Ming-Hao,Qin Meng-Yao,Zhao Yuan-Di
In this study, composite nanoparticles comprising Au nanoparticle and quantum dots were built and used for contrast-enhanced computed tomography imaging (CT) and fluorescence dual-mode imaging in vivo. The nanoparticle exhibited good monodispersity and good biocompatibility, and had excellent CT contrast-enhancement effect and fluorescence imaging capability. They were appropriate for being used as dual-mode imaging probe in vivo.
研究领域:
医学影像学
·
纳米医学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）