Metabolites
·
2022
·Vol. 12
·期 12
·1220
·
IF 5.581
Tiansheng Chou,Xiaosheng Huang,Jiawei Liu,Xinhua Liu,Kun Zeng,Zonghui Yan,Shaoyi Mei,Liangnan Sun,Wenqun Xi,Jinglan Ni,Jin Zi,Jun Zhao,Siqi Liu
The quantitative level of steroid hormones (SHs) in some body fluids have been accepted for clinical diagnosis, whereas their distribution in aqueous humor (AH) is unknown yet. Herein, a profiling study was conducted with a total of 171 AH and 107 plasma samples using liquid chromatography coupled with tandem mass spectrometry (LC MS/MS). For the first time, six kinds of SHs in AH were quantitatively estimated, and their abundances were ranked at cortisol (F), corticosterone (COR), androstenedione (A2), and 11-deoxycortisol (11DOC). The corresponding abundance of all SHs in AH was significantly lower than those in plasma, while there was a lack of a proportional relationship with the abundance of plasma SHs. Dehydroepiandrosterone sulfate, the most abundant plasma SH, was undetectable in AH, implying that the blood–aqueous barrier might specifically block its transferral. Axial myopia generally results from many factors throughout the entire eye from tissues and molecules; furthermore, the correlation of AH SHs and axial myopia was assessed to look for their indication in such myopia. The panel with five kinds of AH SHs (F, COR, CORT, ALD and A2) was functional as a discriminator for axial myopia and control. The abundance of SHs, therefore, has a specific distribution in AH and can potentially contribute to axial myopia.Keywords:steroid hormone;aqueous humor;blood–aqueous barrier (BAB);axial length (AL)
研究领域:
眼科学
·
内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
iScience
·
2025
·
IF 4.1
Kaiyun Zhang,Lei Yu,Laiqiang Qu,Na Hui,Li Chen,Jiameng Wang,Hong Yan
Microplastics (MPs), an emerging global pollutant, pose potential risks to human health and have garnered increasing attention. Previous research has identified MPs in various human tissues and organs, but not in the aqueous humor of the eyes. This study used pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) to explore MPs in aqueous humor. Five types of MPs—polyethylene (PE), polyvinyl chloride (PVC), polypropylene (PP), polyamide 66 (PA66), and polystyrene (PS)—were found, with PE and PVC being most common. PP was more prevalent in children, while PA66 was more common in adults. MPs abundance generally followed the trend: adults > children > elders among age groups, and females > males between gender groups. Notably, this study is the first to confirm MPs in human aqueous humor, providing a foundation for future research on their impact on intraocular health and enhancing our understanding of the MPs’ body distribution.
研究领域:
环境科学
·
医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Xin Xie,Dan Liu,Weili Wang,Juan Xiang,Minghui Yang,Guokun Liu
The abundance of brain-derived neurotrophic factor (BDNF) in aqueous humor (AH) is an ideal biomarker for the diagnosis of glaucoma, a chronic progressive optic neuropathy and the most frequent cause of irreversible blindness. The difficulty of AH-based BDNF detection is from the small amount of extracted AH in a paracentesis (<100 μL) and the ultra-low abundance of BDNF. In this work, we systematically studied the non-specific adsorption of biofluids on the bare gold electrode by electrochemistry and Raman spectroscopy techniques, revealing the unexpected negative correlation of the extent of non-specific adsorption with the size of the electrode. Based on it, a simple microelectrode-based sensor without the introduction of the blocking layer was developed for the detection of BDNF in the AH sample. Using electrochemical impedance spectroscopy (EIS) and extracting the changes of electron-transfer resistance of the electrochemical probe [Fe(CN)6]3–/4– on the sensor surface, the BDNF was quantified. The dynamic range was from 0.5 to 50 pg·mL–1, with a detection limit of 0.3 pg·mL–1 and a sample consumption of 5 μL. The real AH sample analysis confirmed the significant decrease of BDNF abundance in the AH of glaucoma patients. Our microelectrode-based EIS sensor displayed prominent advantages on simplified preparation, sensitive response, and low sample consumption. This AH-based BDNF analysis is expected to be used for the screening and diagnosis of glaucoma, especially for the high-risk population who have ocular diseases and have to undergo surgeries.
研究领域:
生物医学工程
·
分析化学
·
眼科
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2021
·
IF 3.517
Xia Zhao,Sihao Liu,Yuemei Han,Yuqin Wang,Quankui Lin
The main reason for the failure of glaucoma drainage devices is the chronic inflammatory reaction caused by the poor biocompatibility of biomaterials, which leads to the migration and proliferation of scleral fibroblasts surrounding the devices thus forming the encapsulation that will block the aqueous humor drainage channel. In order to inhibit the reaction of scleral fibroblasts after glaucoma drainage device implantation, this study designed and fabricated a 5-fluorouracil (5-FU) loaded chitosan microtube (CMT) for glaucoma aqueous humor drainage. The CMTs were made by template based adsorption-precipitation method using chitosan with excellent biocompatibility as raw material due to its characteristic of pH dependent solubility. The physical properties of CMTs were investigated. The in vitro and in vivo biocompatibilities were studied as well. The developed drainage device is expected to possess the dual function of reducing intraocular pressure and inhibiting excessive fibrosis after glaucoma drainage device implantation, thereby providing a novel way for the research of glaucoma drainage devices.
研究领域:
药学
·
生物医学工程
·
眼科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2014
·
IF 4.195
Jie Lou,Wenjing Hu,Rui Tian,Hua Zhang,Yuntao Jia,Jingqing Zhang,Liangke Zhang
This study aimed to optimize and evaluate a thermoresponsive ophthalmic in situ gel containing curcumin-loaded albumin nanoparticles (Cur-BSA-NPs-Gel). Albumin nanoparticles were prepared via a desolvation method, and the gels were prepared via a cold method. The central composite design and response surface method was used to evaluate the effects of varying Pluronic ® F127 and Pluronic ® F68 concentrations on the sol–gel transition temperature, which is an indicator of optimum formulations. The optimized formulation was a free-flowing liquid below 30.9°C that transformed into a semi-solid gel above 34.2°C after dilution with simulated tear fluid. Results of the in vitro release and erosion behavior study indicated that Cur-BSA-NPs-Gel achieved superior sustained-release effects and that incorporation of albumin nanoparticles exerted minimal effects on the gel structure. In addition, in vivo ophthalmic experiments employing Cur-BSA-NPs-Gel were subsequently performed in rabbits. In vivo eye irritation results showed that Cur-BSA-NPs-Gel might be considered safe for ophthalmic drug delivery. The in vivo study also revealed that the formulation could significantly increase curcumin bioavailability in the aqueous humor. In conclusion, the optimized in situ gel formulation developed in this work has significant potential for ocular application.
研究领域:
药学
·
纳米技术
·
眼科学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2022
·Vol. 10
·期 6
·
IF 9.043
Jun Ren,Menghuan Yu,Wenjin Gao,Chuanfan Ding,Shengjie Li,Shaoning Yu,Wenjun Cao
Prompt clinical diagnosis and antimicrobial therapy are key to managing infective endophthalmitis. The small volume of aqueous humor, low bacterial counts, and empirical medication by physicians make existing diagnostic methods time-consuming and imprecise. Here, we investigated the feasibility of combining Fc-containing mannose-binding lectin-coated Fe3O4 (Fc-MBL@Fe3O4) enrichment with matrix-assisted laser desorption–ionization time of flight mass spectrometry (MALDI-TOF MS) profiling to identify pathogens in aqueous humor. Aqueous humor aspirated from freshly enucleated porcine eyes was treated with different inocula of Staphylococcus aureus, Staphylococcus epidermidis, and Klebsiella pneumoniae. We performed identification directly in aqueous humor samples and after short-term culture of micro-LB broth. Aqueous humor endophthalmitis samples were enriched with Fc-MBL@Fe3O4 and analyzed using MALDI-TOF MS. The identification time and minimum bacterial concentration required for identification were determined. The enrichment efficiency of Fc-MBL@Fe3O4 for different bacteria was greater than (87.5 ± 5.0)%. The objects of direct identification include live bacteria and bacteria treated with antibiotics, which can be completed within 1.5 h. The minimum number of bacteria needed for positive identification was 2.20 × 106 CFU. For micro-LB broth culture, the identification of bacteria can be completed within 6.5 to 9.5 h for aqueous humor samples with an initial bacterial count of tens to hundreds.IMPORTANCE Fc-MBL@Fe3O4 capture not only live bacteria in aqueous humor but also bacteria inactivated by antibiotics. Fc-MBL@Fe3O4 combined with micro-LB broth culture significantly reduced the turnaround time (TAT) by more than half a day by shortening the time required for bacterial identification. Our findings demonstrate that combining Fc-MBL@Fe3O4 enrichment with MALDI-TOF MS identification is a fast, sensitive, and efficient analytical method with great potential for identifying pathogens in aqueous humor samples.
研究领域:
临床微生物学
·
质谱分析
·
眼科
·
传染病学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）