INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Xinyue Cheng,Xueyan Sun,Shiqiang Zheng,Wenjia Zhou,Zhen Yuan,Liangliang Zhu,Xi Chen
Direct air capture (DAC) of CO2 is emerging as an important technology to mitigate the environmental challenges posed by excessive carbon emissions. The development of reliable and affordable adsorbents has long been a topic of great interest in the DAC field. In this work, through a quaternization process, inexpensive and readily available plant-based biochars including walnut shell, cornstalk, rice husk, and long-stalked lentil shell were prepared as moisture-swing adsorbents to capture CO2 from ambient air. Among these biochar adsorbents, the most effective one was found to be the quaternized long-stalked lentil shell, whose CO2 adsorption capacity reached 0.88 mmol/g at 25 °C and 50% relative humidity, which is around five times that of previously reported biochar moisture-swing adsorbents (including bamboo cellulose and chitosan aerogel). Interestingly, different from the anion-exchange resin (the most studied moisture-swing adsorbent) whose adsorption capacity decreases progressively with increasing ambient humidity and the optimal adsorption requires a relative humidity below 5%, the adsorbents developed here obtained optimal adsorption performance at 50% relative humidity (at room temperature). This result greatly expands the suitable deployment area of moisture-swing DAC since the relative humidity of most land areas on earth is in the range of 40–80%.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
MOLECULAR BIOLOGY REPORTS
·
2022
·Vol. 49
·期 8
·7665-7676
·
IF 2.742
Jiang Haoran,Wen Xianxin,Zhang Xue,Zhong Xianhua,Li Zhangyong,Zhang Bingyu
Background Hepatocellular carcinoma (HCC) is the main types of primary liver cancer, which shows some abnormal glycosylation, such as the increase of fucose. Lens culinaris agglutinin (LCA), a natural plant lectin that can bind to mannose and fucose, has been reported to be antiproliferative to may tumors. However, the effect of LCA on the vitality and migration ability of human hepatoma cells is not demonstrated. Therefore, the aim of this study is to investigate the effects of LCA on vitality and migration in human hepatoma cells and its potential mechanisms. Methods and results LCA had no significant effect on viability of human hepatoma cells (HCCLM3, MHCC97L and HepG2) and hepatocytes (L02) by CCK-8 kit, but it could inhibit human hepatoma cells migration significantly without affecting hepatocytes by Transwell method. Sugar inhibition assay was used to verify the possible binding site between LCA and human hepatoma cells. The result showed that Mannose- and fucose- related sites were associated with LCA inhibiting human hepatoma cells migration. Moreover, LCA could affect HCCLM3 migration by activating ERK1/2 and JNK1/2/3 signalling pathways. LCA did not affect MMP-2 and MMP-9 of HCCLM3 through gelatinase zymography. However, the results of immunofluorescence standing showed that LCA could reduce the F-actin formation in HCCLM3 via ERK1/2 and JNK1/2/3 signalling pathways. Conclusions LCA might inhibit human hepatoma cell migration by reducing the F-actin formation via the mannose and fucose-mediated ERK1/2 and JNK1/2/3 signalling pathway. This result will deepen people’s understanding on plant lectin as a drug in tumor glycobiology.
研究领域:
肿瘤生物学
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分子生物学
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细胞生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Liyuan Rong,Mingyue Shen,Huiliang Wen,Yanming Ren,Wenhao Xiao,Jianhua Xie
Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO 2 -N) and Mesona chinensis Benth polysaccharide (MCP). The effects of TiO 2 -N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO 2 -N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO 2 -N were found in XRD patterns of composite films. Contact angle of HBS/TiO 2 -N/M3 film increased from 65.6° to 90.9°, which illustrated that TiO 2 -N and MCP effectively enhanced hydrophobicity of films. TiO 2 -N and MCP positively affected anti-UV light ability of HBS films by resisting most of invisible light. Furthermore, stable and compact network structures were formed by the synergistic effect of TiO 2 -N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly. This study prepared a nanocomposite HBS-based films based TiO 2 -N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.
研究领域:
食品科学
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材料科学
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DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 32
·19813-19818
·
IF 3.361
Yazhou Qin,Yuanzhao Wu,Binjie Wang,Jiye Wang,Xingsen Zong,Weixuan Yao
Branched Au nanoparticles (Au NPs) can significantly enhance the Raman signal of trace chemical substances, and have attracted the interest of researchers. However, there are still challenges to accurately prepare the morphology of branched Au NPs. In this work, we have successfully prepared sea urchin-like Au NPs and Au nanowires by using the seed-mediate growth method, with cetyltrimethylammonium bromide (CTAB) and glutathione as ligands, and ascorbic acid as a reducing agent. Using Au NPs with a tetrahexahedron (THH) morphology as seeds, and by simply changing the concentration of glutathione, we explored the growth process of sea urchin-like Au and Au nanowires. At low concentrations of glutathione, Au NPs will preferentially grow along the edges and corners of the THH Au seed, forming a core/satellite structure. As the concentration of glutathione increases, Au NPs will grow along the direction of glutathione, forming sea urchin-like Au NPs. To further increase the concentration of glutathione, we will prepare Au nanowires. In addition, we use the prepared Au NPs as a substrate material for surface-enhanced Raman (SERS) high-sensitivity detection. By using 4-aminothiophenol (4-ATP) as the test molecule, we evaluated the SERS effect of the prepared Au NPs with different morphologies. The results showed that sea urchin-like Au NPs have the best enhancement effect. The lowest concentrations of Rhodamine 6G and 4-ATP were 10−10 M and 10−12 M, respectively, using sea urchin Au NPs as the base material. Furthermore, we conducted a highly sensitive SERS detection of the poison atropine monohydrate, and the lowest detected concentration was 10−10 M.
研究领域:
纳米材料科学
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分析化学
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毒理学
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产品关联
DOI
PubMed
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2021
·Vol. 85
·期 4
·798-804
·
IF 2.043
Yang Yanfei,Li Yabin,Han Jinli,Wang Yanfen
Gastrodin has shown the potential as an anticonvulsant. Epilepsy is a neurological disease with significant effects in children. In the current study, the therapeutic potential of gastrodin in handling pediatric epilepsy was explored by focusing on the AMPK/PPARα pathway. Three-week-old Sprague–Dawley rats were subjected to lithium-pilocarpine method to induce epileptic symptoms and then administrated with gastrodin. The effects of gastrodin on rats were first assessed using electroencephalogram (EEG) recording, Racine classification, Morris water maze test, and histological staining. The levels of BDNF and NGF, and the activity of AMPK/PPARα were measured. Based on the results of EEG, behavior analyses, and histological staining, epileptic symptoms were significantly alleviated by gastrodin. Moreover, the administration of gastrodin also suppressed the levels of BDNF and NGF, and activated the AMPK/PPARα pathway. In conclusion, our results demonstrated that gastrodin contributed to the alleviation of pediatric epilepsy by activating AMPK/PPARα signaling transduction.
研究领域:
神经科学
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药理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Fanghua Guo,Li Peng,Hua Xiong,Jiaqi Wang,Rong Tsao,Xin Peng,Li Jiang,Yong Sun
Polyphenol-rich Laird lentil hulls are a byproduct of lentil processing. In the present study, free and bound polyphenols in lentil hulls were analyzed with UHPLC-LTQ-OrbiTrap-MS2, and the anti-inflammatory mechanism of their digestive products was explored based on the NF-κB and Keap1-Nrf2 signaling pathways in the HT-29 cell model. In summary, a total of 27 polyphenols and 5 nonphenolic constituents were identified in free and bound fractions, and among them, catechin glucoside, kaempferol tetraglucoside, procyanidin dimer, and dihydroxybenzoic acid-O-dipentoside were the main polyphenols in the digestive products. These digestive products could reduce inflammatory mediators and exert anti-inflammatory activity by inhibiting NF-κB and activating Keap1-Nrf2 signaling pathways, and there was crosstalk between them, which was a mutual inhibition effect. The results show that polyphenols in lentil hulls are a good source of anti-inflammatory ingredients and have a promising development potential.
研究领域:
食品科学
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药理学
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分子生物学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
HIPPOCAMPUS
·
2023
·
IF 3.5
Shicheng Jiang,Bei Liu,Kaiwen Lin,Lianjun Li,Rongrong Li,Shuo Tan,Xinyu Zhang,Lei Jiang,Hong Ni,Yuanyuan Wang,Haihu Ding,Jing Hu,Hao Qian,Rongjing Ge
Numerous epilepsy-related genes have been identified in recent decades by unbiased genome-wide screens. However, the available druggable targets for temporal lobe epilepsy (TLE) remain limited. Furthermore, a substantial pool of candidate genes potentially applicable to TLE therapy awaits further validation. In this study, we reveal the significant role of KCNQ2 and KCNQ3, two M-type potassium channel genes, in the onset of seizures in TLE. Our investigation began with a quantitative analysis of two publicly available TLE patient databases to establish a correlation between seizure onset and the downregulated expression of KCNQ2/3. We then replicated these pathological changes in a pilocarpine seizure mouse model and observed a decrease in spike frequency adaptation due to the affected M-currents in dentate gyrus granule neurons. In addition, we performed a small-scale simulation of the dentate gyrus network and confirmed that the impaired spike frequency adaptation of granule cells facilitated epileptiform activity throughout the network. This, in turn, resulted in prolonged seizure duration and reduced interictal intervals. Our findings shed light on an underlying mechanism contributing to ictogenesis in the TLE hippocampus and suggest a promising target for the development of antiepileptic drugs.
研究领域:
神经科学
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药理学
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分子生物学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
Current Drug Therapy
·
2024
·Vol. 19
·期 2
·239-249
·
IF 0.3
Singh Neelam,Gupta Puneet,Goel Radha,Narayan Chaitanya V.,Sagar Deepak,Gupta Aanchal
Introduction: Medicinal plants are essential in the treatment of a wide range of illnesses. Dolichos lablab, also known as Hyacinth Bean, is a popular legume crop in India that is rich in natural bioactive compounds. In recognition of its importance in many skin therapies, a
hydrogel was prepared. The primary goal of topical formulations is to provide drug contact with the skin while reducing overall absorption. The aim of this study was to evaluate the healing effect of Dolichos lablab extract hydrogel on full-thickness wounds in rats. Method: Wounds
were created on the dorsal surface of Male Albino Wistar rats. Next, the animals were divided into four groups (n = 12). Each group was treated with 200 mg of the allotted topical treatment of blank gel, Dolichos hydrogel and marketed betadine gel. The treatments were applied immediately after
the injury and twice daily for up to 14 days. Animals were euthanized on day 14 and some parameters were analyzed such as wound closure rate, Inflammatory cytokines level, oxidative stress profile, collagen hydroxyproline and hexosamine concentration, macroscopic and histopathological evidence.
Results: The findings indicate that Dolichos lablab extract has potent wound healing ability, as evidenced by improved wound closure and tissue regeneration, as well as histopathological parameters. Dolichos hydrogel increased the skin's hydroxyproline level, antioxidant potential,
wound contraction, and anti-inflammatory activity by modulating the production of the cytokines TNF-α, IL-1β, and IL-6. This affirms the potency of the wound-healing properties of Dolichos lablab extract. Conclusion: Dolichos lablab hydrogel is a low-cost herbal
medicine which can aid in tissue repair.
研究领域:
药理学
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伤口愈合
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草药医学
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动物模型
✓ 已验证
DOI
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Lin Chen,Xin Kong,Ruina Zhou,Jinhang Hu,Rui Zhou,Zhongxing Song,Zhishu Tang,Mei Wang
ABSTRACT Background Total flavonoids from Astragali Complanati Semen (TFACS), the main active ingredients in Astragali Complanati Semen (ACS), have been shown to have a protective effect on chronic liver injury (CLI), but the hepatoprotective targets and signalling pathways involved are unclear. Purpose The aim of our study was to identify the anti-CLI targets and signalling pathways of TFACS and to comprehensively elucidate its mechanism of action via proteomics analysis combined with in vivo and in vitro experiments. Methods A CLI mouse model was generated via intraperitoneal injection of carbon tetrachloride (CCl 4 ) (CCl 4 : olive oil = 1:4, 2 mL/kg, twice a week for 6 weeks). The hepatoprotective effect of TFACS was assessed by observing the pathological structure of the liver and analysing indicators of liver function. The key pathways and targets related to the hepatoprotective effect of TFACS were identified via 4D-label-free quantitative proteomics technology and further verified via in vivo indicator validation and in vitro cell experiments. Results TFACS administration significantly normalized the histopathological structure and function of the liver, decreased the levels of inflammatory factors and oxidative stress indicators, and reduced the iron staining area and the levels of hepcidin and iron in the liver compared with those in the CLI model. A total of 424 differentially expressed proteins (DEPs) were identified between the TFACS and model groups, and these DEPs were enriched in the focal adhesion, PI3K-Akt, and ferroptosis pathways. Akt1, Pik3ca, NF-κB p65, Itga5, Itgb5, Itga6, Prkca, Fn1, Tfrc, and Vdac3 were identified as key targets of TFACS. TFACS administration significantly reversed the changes in the gene and protein expression of the key targets compared with those in the model group. In addition, TFACS treatment significantly reduced the levels of inflammatory cytokines and inhibited Akt1, NF-κB p65 and FAK activation in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. In an erastin-induced L-O2 ferroptosis cell model, treatment with TFACS normalized the mitochondrial structure, reduced the protein levels of Tfrc and Vdac3, inhibited lipid peroxidation, and reduced the amount of Fe 2+ in the mitochondria. Conclusion TFACS protected against CLI, and its mechanism of action may be related to inhibition of the focal adhesion, PI3K/Akt and ferroptosis signalling pathways.
研究领域:
药理学
·
蛋白质组学
·
肝病学
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Huo Jinxi,Sun Yuqing,Pan Meiliang,Ma Huanyan,Lin Tianbao,Lv Zhiqiang,Li Yougui,Zhong Shi
Sanghuangprous vaninii is a wood-inhabiting fungus, and its mycelium and fruiting body show excellent medicinal values. Mulberry is one of the major hosts of S. vaninii, however, the mechanism of mulberry affecting the growth of S. vaninii has not been reported. In the present study, a mulberry-inhabiting strain of S. vaninii was selected to explore the effects of mulberry branch extracts (MBE) on the growth of the strain. Results showed that MBE could significantly promote the growth of S. vaninii mycelium at the concentration of 0.2 g/l. After 16 days of liquid culture, the dry weight of mycelium in 0.2 g/l MBE medium was higher by three times compared with that in the control. The non-targeted metabonomic analysis of the culture medium at different culture times and concentrations was conducted to find the key components in MBE that promoted the growth of S. vaninii mycelium. Under the different concentrations of MBE culture for 10 and 16 days, 22 shared differential metabolites were identified. Next, in accordance with the peak value trend of these metabolites, HPLC–MS and liquid culture validation, four components derived from MBE (i.e., scopoletin, kynurenic acid, 3,5-dihydroxybenzoic acid and 2,4-dihydroxybenzoic acid) could significantly increase the growth rate of mycelium at the concentration of 2 mg/l. Transcriptomic and qRT-PCR analyzes showed that MBE could upregulate hydrolase-related genes, such as serine–glycine–asparaginate–histidine (SGNH) hydrolase, alpha-amylase, poly-beta-hydroxybutyrate (PHB) depolymerase, glycosyl hydrolase family 61, cerato-platanin protein and Fet3, which might enhance the nutrient absorption ability of S. vaninii. Importantly, MBE could significantly increase the content of harmine, androstenedione and vesamicol, which have been reported to possess various medicinal effects. Results suggested that MBE could be an excellent additive for liquid culture of S. vaninii mycelium, and these hydrolase-related genes also provided candidate genes for improving the nutrient absorption capacity of S. vaninii.
研究领域:
真菌学
·
代谢组学
·
转录组学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Bing-Hui Zou,Juan Yi,Yu-Fei Yao,Wen-Juan Li,Ming-Yong Xie
White hyacinth bean polysaccharide (WHBP) was demonstrated to be effective in weight control and hypolipidemic regulation. Oral Superabsorbent Hydrogels (OSH), a class of dietary hydrogels capable of absorbing large amounts of water in the gastrointestinal tract, have emerged as an innovative carrier system for treating obesity. Here, combination of sodium alginate and inulin was employed to prepare OSH containing WHBP (named WHBP-OSH). Inulin was selected to improve characteristics of WHBP-OSH, and 5 concentrations of inulin (0–20 %) were tested in this work. Results showed that 10 % inulin was optimal for achieving superior encapsulation efficiency, mechanical properties and swelling capacity. Moreover, digestive behaviors suggested that WHBP-OSH presented significant water absorption and swelling, in addition to stability in both stomach and small intestine regions, thereby achieving colon-targeted delivery of WHBP. Subsequently, a 17-week high-fat diet-induced obesity mouse model was used to address roles of WHBP-OSH in obesity, and results showed that WHBP-OSH resulted in higher levels on weight reduction, Lees index, Body Mass Index (BMI) and adipose index than did OSH alone in obese mice, indicating that WHBP enhanced the anti-obesity effects of OSH. Mechanically, anti-obesity effects of WHBP-OSH were associated with decreasing eating behavior by suppressing the appetite reward and motivation aspects of obese mice. Together, WHBP-OSH containing 10 % inulin could develop a promising colon-targeted OSH, and showed significant potential as a weight management strategy via reducing eating behavior.
研究领域:
药学
·
食品科学
·
生物医学工程
·
营养学
·
高分子科学
·
肥胖研究
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL GENETICS
·
2024
·
IF 2.1
Dai Jie,Shen Hai-lin,Li Jia,Zhou Yong,Dong Zheng-xie,Zhu Xiang-yang
Status epilepticus is a severe neurological emergency that often leads to long-term neuronal damage and functional impairment. Gastrodin is a compound widely used in traditional Chinese medicine with potential neuroprotective effects. This study aims to investigate the effects of GAS on neuroinflammation and injury caused by LiCl/pilocarpine-induced SE in young rats. SE in rats was induced using the LiCl/pilocarpine model. Morris water maze and Y-maze experiments were used for the behavioral test of rats. Enzyme-linked immunosorbent assay was utilized to quantify the levels of interleukin (IL)-1β, IL-6, and IL-8 levels, and biochemical kits assessed the levels of malondialdehyde, superoxide dismutase and glutathione peroxidase (GSH-px) in hippocampus tissues. Additionally, Western blot analysis was performed to evaluate the protein expression levels of p-p65, p65, p-IκBα and IκBα, which are key factors of the nuclear factor kappa B (NF-κB) signaling pathway. Compared to the control group, the SE group rats exhibited reduced learning and memory abilities. Markedly elevated levels of inflammatory factors (IL-1β, IL-6, and IL-8). The expression levels of p-p65 and p-IκBα were significantly upregulated, while IκBα levels were notably decreased. Following GAS treatment, the latency of seizure onset was significantly shortened, the incidence of SE was significantly reduced and the severity of nerve injury was alleviated. Additionally, both the inflammation levels and the oxidative stress were significantly decreased, primarily through inhibition NF-κB signaling pathway. These findings suggest that GAS may be a potential therapeutic agent for treating SE.
研究领域:
神经科学
·
药理学
·
传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）