ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Tingting Liu,Yuhao Li,JingJing Gu,Lei Zhang,Feiyue Qian,BinRong Li,Xuedong Wang
Modulation of the nanozyme's catalytic activity is crucial for its real applications in detecting target analytes. Herein, we fabricated the nanocomposite (NSC/Co 6 Ni 3 S 8 ) of N, S co-doped carbon and Co 6 Ni 3 S 8 by a facile sol-gel approach. Compared to NSC/Ni 9 S 8 , NSC/Co 6 Ni 3 S 8 with bimetallic active sites displayed better enzyme-mimetic activity. This nanocomposite could catalyze O 2 to form ·O 2 − and oxidize colorless 3, 3′, 5, 5′-tetramethylbenzidine (TMB) into blue oxTMB. The other two free radicals (h + and ·OH) played minor roles during the catalytic reaction. Hg 2+ could integrate with S 2− to form HgS and the surface charges of O 2 were transferred to Hg 2+ to promote O 2 adsorption. DFT theoretical calculations highlight that the main reasons for the enhancing effect of Hg 2+ on color development results from electron transfer and increased adsorption energy of O 2 molecules onto the surface of NSC/Co 6 Ni 3 S 8 . By employing the oxidase-like activity of NSC/Co 6 Ni 3 S 8 and Hg 2+ -triggered promoting effect, a colorimetric sensing platform was established for Hg 2+ assay with a linear range of 10–200 μg/L and detection limit of 3 μg/L. Through integration with a smartphone-based APP “Thing Identify” software, a visual colorimetric assay for Hg 2+ was constructed with a detection limit of 5 μg/L. Compared to the data detected by the mercury vapor meter, the relative recoveries of 92.4–108.1% evidenced the higher accuracy of this smartphone-based visual detection. Overall, the NSC/Co 6 Ni 3 S 8 -based colorimetric assay is convenient, rapid, and visual, and can be applied for routine monitoring of Hg 2+ in real-world waters under outdoor conditions.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Qiangqiang Fu,Tao Qi,Ze Wu,Yongjian He,Shujuan Guan,Shihua Luo,Qi Zhang,Wenfeng Luo,Wei Xiao,Bo Situ,Lei Zheng
Anemia affects over 2 billion people worldwide, with the heaviest burden borne by women and children. At present, anemia is diagnosed by measuring hemoglobin (Hb) levels, which must be done in hospitals or commercial laboratories by skilled operators. In this work, we report a portable, affordable ($3), easy-to-operate (1 min) and accurate smartphone-based Hb analyzer (SHbA) that uses a drop of finger-pricked blood for anemia point-of-care test (POCT) applications. POCT of Hb was achieved using a smartphone ambient light sensor (ALS) to accurately measure the absorbance of colorimetric Hb biochemical analysis reagents in a microcuvette, as well as an Android-based application for results analysis. SHbA validation results agreed well with those reported by a hematology analyzer, and the SHbA has an anemia diagnosis sensitivity of 95.4% and specificity of 96.3% for venous blood (n = 360) and a sensitivity of 96.39% and specificity of 95.58% for fingertip blood (n = 475). In addition, SHbA exhibits excellent performance in the diagnosis and treatment guidance of anemia high-risk populations, including tumor chemotherapy patients (n = 424), pregnant women (n = 214) and thalassemia patients (n = 208). Importantly, volunteer self-testing results (n = 20) indicate that SHbA can be used for home-based anemia diagnosis and monitoring. SHbA has the advantages of high sensitivity and specificity while being cheap and easy to operate, making it widely applicable for the diagnosis and treatment of anemia, especially for high-risk patients in areas with poor medical resources.
研究领域:
医学
·
生物化学
·
诊断技术
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jia Wu,Huimei Liang,Shulan Zeng,Shulin Zhao,Fanggui Ye
Pesticide residues pose significant risks to consumer health. Therefore, it is essential to develop convenient and practical detection methods. Nanozyme colorimetric sensing is widely used for pesticide detection. However, improving its sensitivity and selectivity remains a major challenge. In this study, a smartphone-assisted portable test swab fabricated using CuWO 4 as the extractant, combined with solid-phase extraction (SPE), was designed for the colorimetric detection of thiram (THR). Based on the specific binding between the Cu sites in CuWO 4 and THR, interference was eliminated after the SPE to improve selectivity. Moreover, the sensitivity improved after SPE enrichment. Additionally, THR enhanced the peroxidase-like activity of CuWO 4 , whereas tungsten as a co-catalyst further boosted the nanozyme activity, thereby improving the detection sensitivity (limit of detection: 19 nM). The experimental results demonstrate that this method exhibits high stability and good selectivity, with recoveries in real samples ranging from 90.8 % to 112.4 %.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiaoli Zhang,Zhaoguang Wang,Jing Yang,Yingying Li,Cheng Lu,Yanqiang Hao,Guanbo He,Yongjian Zhang,Qifang Song,Jun Long,Jiajie Liang,Yong Tang
Currently, producing NPSH after HPV infection of cells has been confirmed. These NPSH-containing substances accumulate around the urethral opening and are subsequently washed out with urine. Therefore, indirect detection of HPV infection by assessing NPSH levels in urine is feasible, but it has not been reported in detail so far. Here, an assay using phosphotungstic acid to oxidise and produce colour changes by NPSH in urine was developed. This assay enabled the rapid, non-invasive identification of HPV infection by detecting the metabolic byproduct NPSH produced by HPV-infected cells. Employing a smartphone-based device, developed using an ambient light sensor, reduces the cost and simplifies the operation associated with the colourimetric assay. The colourimetric assay was used to detect L-cysteine and L-ascorbic acid standard substance (as NPSH mimics), the limited of detection were 0.12 mM and 31.25 μM, respectively, with high reproducibility and stability. When this colourimetric assay was used to evaluate urine samples from individuals suspected of HPV infection, along with other at-home self-screening methods for HPV nucleic acid detection in urine, showed comparable sensitivity and specificity. Compared with nucleic acid detection in urine, this colourimetric assay is cost-effective, user-friendly, amenable to self-sampling, and enables testing at one’s convenience and location of choice, which is more suitable for home self-testing or population self-screening.
研究领域:
医学检验
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Linshan Zhang,Deshun Huang,Pengxiang Zhao,Guozong Yue,Luming Yang,Weihua Dan
A simple and portable colorimetric sensor for colorimetric detection of UO 2 2+ in aqueous solution based on vinylphosphonic acid functionalized gold nanoparticles (VPA-AuNPs) has been developed. The VPA-AuNPs solution was prepared by sodium borohydride reduction in the presence of vinylphosphonic acid. The addition of UO 2 2+ would induce aggregation of VPA-AuNPs, resulting in the color change from wine-red to blue, and red-shift of the ultraviolet–visible (UV–vis) spectra. The UO 2 2+ assay based on VPA-AuNPs showed good selectivity and sensitivity, with a limit of detection to be approximately 2.0 μM by naked eyes and 1.07 μM by UV–vis (S/N = 3) respectively. Additionally, a smartphone with a free application named “ PhotoMetrix ” was employed to estimate the color intensities (red, green, blue value) of VPA-AuNPs in the presence of UO 2 2+ with different concentrations, and the concentration of UO 2 2+ samples could be conveniently exported by the calculated univariate calibration curves . This method shows good feasibility for on-site UO 2 2+ detection in an aqueous solution.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
·
2018
·Vol. 42
·期 2
·e12976
·
IF 1.955
Ning Yang,Liang-Liang Xie,Chen Pan,Min-Feng Yuan,Zhi-Han Tao,Han-Ping Mao
Ubiquitous food security control is desirable. However, the analysis methods, such as those for milk, are highly based on manual analysis, leading to low efficiency. Thus, we report a multilayer paper-based microfluidic chip transforming chromatic readout to electric signals. First, colloidal gold immunochromatographic assays (GICA) are used to achieve the colorimetric occurrence. Secondly, the test (T) lines and control (C) lines are patterned to encode different EAN-8 barcode. Furthermore, accurate scanning is accomplished by the automatic threshold segmentation. These technologies make the digital determination of melamine (MEL) and chloramphenicol (CAP) by phone readily accessible. Results indicate that the accurate machine-scanning can be assured when MEL and CAP is above 30 ng/mL and 6 ng/mL in milk respectively. The detection time is under 8 min, and the stable storage life is over 90 days. Therefore, this simple chip allows rapid, machine-readable, electric-recordable diagnostic directly from chromatic assays with satisfying stability, specificity and accuracy. Practical applications This article illustrates a method used to determine the concentration of melamine and chloramphenicol in milk. When melamine and chloramphenicol is above 30 and 6 ng/ml in milk, the produced EAN-8 barcode can be recognized with high accurate level, compared with international standards, the results are acceptable and sensitive. This method is highly intellectual and environmentally friendly, with the disposable materials and instant smartphone-readable EAN-8 results patterns. This article demonstrates a multilayer paper chip based on EAN-8 encoding rules, colloidal gold nanoparticles, and immunochromatographic assays. The EAN-8 format assay is achieved by color-development occurrence of colloidal GICA in different encoding areas of EAN-8 code, and the analysis device, namely smartphone, is accessible by almost every individual. Therefore, this chip can pave a potential way for massive health control and digital automated analysis for daily life and industrial manufacture.
研究领域:
食品安全
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2022
·
IF 2.731
Xuechun Yan,Mingbin Liu,Hongbo Li,Suqin Wang
Silver is widely used, but the pollution caused by silver ions is also a problem that cannot be ignored. Detection of heavy metal ions based on bulky and costly analytical instruments had been developed very maturely in the laboratory. At present, the main detection methods of silver ion concentration include inductively coupled plasma mass spectrometry, atomic absorption spectrometry and inductively coupled plasma/atomic emission spectrometry. However, these testing methods have many problems to be solved, such as the high of instruments, high operating cost, numerous operational requirements, long testing time and complex processing technology. Nowadays, with the rapid and powerful development of smartphone technology, the new analytical methods based on smartphones equipped with a camera are being carried in the certain applied ideas. Here, the paper introduces a hand-held detection platform smartphone-based that allows the quantification of silver(I) ions with the detection limit of 0.659 mM. The Colour Grab software of android smartphone was applied to obtain R, G, B values of the photo of Rhodamine B solution remaining fluorescence, and then calculated the corresponding grey values by grey formula. The detection platform based on smart phones has the characteristics of cost-effective design and simple operation, as well as high sensitivity and selectivity. Therefore, compared with other detection methods, this method can be widely and conveniently applied to the detection of silver ions in the environment.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Lanlan Li,Xiaoyu Dong,Jihong Chen,Jing Xiao,Li Zeng,Zhengwen Huang,Zhirong Zou
A rapid and easy-used colorimetric method was developed for on-site and rapid analysis of Cr(VI). Under acidic condition, Cr(VI) was reacted with 1, 5-diphenylcarbazide (DPC) and a purple-red complex was formed subsequently, inducing the color changed from colorless to purple-red, this color variance can be detected by ultraviolet–visible spectrophotometer (UV–Vis), smartphone and even naked-eye. For realizing the goal of on-site analysis, this method was integrated in a three dimensional (3D) printed analytical kit, smartphone was used as a portable detector. Under the optimized conditions, limit of detections (LODs) of 0.0014 μg/mL and 0.0016 μg/mL were obtained by smartphone and UV–Vis, respectively. Concentration of Cr(VI) as low as 0.05 μg/mL was still visible by naked-eye. This colorimetric system was further applied to the analysis of several environmental water samples, recoveries of 90–107 % were obtained. It is a promising portable analytical device/system for Cr(VI) on-site and rapid analysis.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Beibei Zhao,Xinfang Liu,Zheng Cheng,Xu Liu,Xiaoyu Zhang,Xun Feng
Pymetrozine (PYM) is an effective pyridine insecticide for controlling aphids, while its residues pose a serious threat to human health. Herein, a europium complex (Eu-DBPA, DBPA represents deprotonated 2,5-dibromoterephthalic acid ligand) probe was prepared for the detection of PYM via fluorescence quenching . The detection process has the advantages of short response time (2 min), wide linear range (0–4 and 4–45 mg/kg) and low detection limit (2.2 μg/kg). Furthermore, a portable detection platform was designed by integrating Eu-DBPA-based paper strip with smartphone and applied for the visual detection of PYM in real cucumber, tomato, cabbage and apple samples, obtaining satisfactory recovery (99.00 %–107.00 %) and low standard deviation (RSD < 3.4 %). In addition, a logic gate device was designed to simplify the detection process. The smartphone-integrated paper-based probe detection platform provides a new strategy for intelligent and online identification of hazards in environmental and biological samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 18
·2102-2107
·
IF 2.073
Yuhao Xiong,Fang Li,Jingwen Wang,Ailing Huang,Mei Wu,Zhi Zhang,Dongjian Zhu,Wei Xie,Zhenhua Duan,Linjing Su
The determination of selenium (Se) has garnered increasing interest because of its importance to life as a nutrient element as well as its toxicological effects. Herein, a simple multimodal colorimetric method was developed for the detection of selenium. Firstly, thiol cotton is used as the functional material for simple solid-phase extraction separation of Se(IV). Then, the enriched Se(IV) oxidizes I− ions into I2 species, leading to the formation of linear triiodide ions that intercalate into the coil of starch molecules, producing a blue color. Based on the changes in the intensity of the blue color, multimodal detection methods using spectrophotometric detection, smartphone app-based colorimetric detection, and naked-eye colorimetric detection were developed for selenium detection. The spectrophotometric detection mode exhibited a linear response from 0.01 to 8.0 μg mL−1 with a detection limit of 0.0047 μg mL−1; the smartphone app-based colorimetric detection mode was linear from 1.0 to 6.0 μg mL−1 with a detection limit of 0.05 μg mL−1; and selenium levels of 0.1 μg mL−1 could be clearly distinguished by the naked eye. The colorimetric detection method was demonstrated to be applicable for the detection of selenium in real water and vegetable samples, giving recoveries of 94.1 to 108.4%.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analysis and Testing
·
2021
·Vol. 5
·期 1
·19-29
·
IF 0
Liu Zhong-gang,Xiao Meng,Yang Rong-zhe,Zhou Qiao-qiao,Ye Hui-fen,Yi Chang-qing
This work reports on a smartphone-based quantitative POCT system which consists of a paper-based microarray chip, a homemade LEGO dark-box, and a smartphone installed with custom-designed FChip-APP, for on-site quantitation of Fe 3+ , Vitamin 12 (VB 12 ), and folic acid (FA). Collection and automatic analysis of fluorescence turn-off response of three kinds of fluorescent nanoprobes which are coated onto filter papers by a smartphone-based device is the principle behind this study. The POCT system exhibits good analytical performance for multiplexed analysis of Fe 3+ , VB 12 and FA with a wide linear range (Fe 3+ , 1.0–14.0 μg mL −1 ( R 2 = 0.983); VB 12 , 1.35–40.0 μg mL −1 ( R 2 = 0.991); FA, 2.0–26.0 μg mL −1 ( R 2 = 0.994)) and low detection limit (Fe 3+ , 0.35 μg mL −1 ; VB 12 , 0.47 μg mL −1 ; FA, 0.56 μg mL −1 ). The cost and weight of the whole system are ~ $220 and ~ 600 g per set, highlighting its cost-effectiveness and portability. The simple operation procedures and user-friendly interface of the FChip-APP validate the ease-of-use feature of the reported system. This versatile smartphone-based quantitative POCT system provides a cost-effective and easy-of-use method for screening the context-specific causes of anemia and thus its effective interventions in the future, especially in resource-limited areas.
研究领域:
医学检测
·
生物传感
·
移动健康
✓ 已验证
产品关联
DOI
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 10
·4667-4673
·
IF 3.3
Yufei Lei,Yujie Li,Chen Liu,Liqiong He,Qian Li,Lifu Liao,Xilin Xiao
A convenient and portable smartphone-assisted method for the colorimetric detection of uranyl ions (UO22+) in aqueous solutions is proposed using rifampicin and 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP) as chromogenic agents. The addition of UO22+ would cause reaction with chromogenic agents to form complexes, changing the colour from orange to dark red in the rifampicin system and from yellow to light pink in the Br-PADAP system. To achieve the on-site estimation of the trace UO22+ concentration range, digital images of colour changes captured by the smartphone under fixed lighting conditions were imported into the built-in application named “Detect” to obtain HSV (hue, saturation, and value) and HSL (hue, saturation, lightness) information, which could be associated with the concentration of uranyl ions. For more precise laboratory-level detection, ultraviolet-visible (UV-vis) spectroscopy was employed as well. The absorbance of the rifampicin system exhibited strong linearity with the UO22+ concentration in the range from 4 to 50 μM at 375 nm with a method detection limit (MDL) of 3.17 μM by the UV-vis-based method, while that of Br-PADAP system had a linear relationship with the concentration from 0.9 to 7 μM at 562 nm with an MDL of 0.89 μM. The two strategies may be combined to detect UO22+ across a larger concentration range, and this method has been successfully used to analyze real samples including soil around uranium mines.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）