Biosensors-Basel
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2022
·Vol. 13
·期 1
·22
·
IF 5.743
Lingyuan Xu,A. M. Abd El-Aty,Jing Zhao,Xingmei Lei,Xiuyuan Zhang,Yun Zhao,Xueyan Cui,Yongxin She,Fen Jin,Jing Wang,Maojun Jin,Bruce D. Hammock
In this study, a previously unreported 3-((4-(isopropylamino)-6-(methylthio)-1,3,5-triazin-2-yl) amino) butyric acid hapten was designed and synthesized. This maximized the exposure of the antigen-determinant isopropyl of prometryn to the immune system in animals to induce the production of anticipated highly specific anti-prometryn antibodies. The hapten has a molecular weight of 285.37 Da. The compound was confirmed by nuclear magnetic resonance hydrogen spectroscopy (1H NMR), nuclear magnetic resonance carbon spectroscopy (13C NMR), and high-resolution mass spectrometry (HRMS). By using the active ester approach, immunogens and coated antigens were created. Bovine serum albumin (BSA) was used as an immunogen, along with the successfully produced hapten, to immunize mice. The IC50value of mouse monoclonal anti-prometryn antibody (mAb) 7D4 (the quantity of analyte that generated 50% prometryn inhibition) was 3.9 ng/mL. The anti-prometryn mAb was of the IgG1 subclass. The IC20(80% binding level (B/B0) of prometryn)-IC80(20% binding level (B/B0) of prometryn) range of the anti-prometryn monoclonal antibody standard curve working range was 0.9–18.1 ng/mL. The prepared mAb has good characteristics because it can specifically recognize prometryn, and the cross-reaction rates for ametryn, desmetryn, and terbumeton were 34.77%, 18.09%, and 7.64%, respectively. The cross-reaction rate with the other seven triazines was less than 1%. The hapten structure proposed can serve as an additional tool for modulating selectivity in detecting triazines.Keywords:prometryn;hapten;monoclonal antibody;immunoassays
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Haowei Dong,Jingcheng Huang,Zhen Guo,Haifang Wang,Lingjun Geng,Ziping Cai,Ibrahim A. Darwish,Yemin Guo,Xia Sun,Yanfang Wu
Procymidone is frequently detected in vegetables, prompting stringent regulatory limits in many countries and regions. Despite this, the development of rapid and sensitive detection methods remains limited. In this study, an aptamer-based lateral flow assay (LFA) was developed for procymidone detection, utilizing Prussian blue nanoparticles (PBNPs) as amplified signal markers, chosen for their intrinsic color and enzyme-like properties. An aptamer was coupled with the PBNPs to form a signal probe, enabling the construction of a competitive LFA. Under optimized conditions, the LFA achieved a limit of detection (LOD) of 0.35 ng/mL and a visual limit of detection (vLOD) of 0.64 ng/mL. The enzyme-mimetic activity of PBNPs was harnessed and such a catalytic reaction strategy further improved the assay sensitivity, leading to a further improvement of the LOD by 14.3-fold. The assay demonstrated high sensitivity, selectivity, and reproducibility, performing effectively in typical vegetable samples. This study provides a rapid, sensitive and reliable tool for procymidone detection, offering valuable insights and a new strategy for monitoring pesticides.
研究领域:
分析化学
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食品安全
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纳米技术
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Rong-Rong Zhang,Xin-Tian Gan,Jin-Jin Xu,Qiao-Fen Pan,Hua Liu,Ai-Li Sun,Xi-Zhi Shi,Ze-Ming Zhang
A highly effective molecularly imprinted electrochemiluminescence sensor was constructed for prometryn determination in environmental and biological samples by using perovskite quantum dots coated with a molecularly imprinted silica layer (MIP/CsPbBr 3 -QDs) as the recognition and response element. MIP/CsPbBr 3 -QDs were immobilized on a glassy carbon electrode (GCE) through electropolymerization, and the electrochemiluminescence (ECL) response of MIP/CsPbBr 3 -QDs could be motivated under the condition of H 2 O 2 as co-reactant. ECL signal was selectively quenched with prometryn by hindering electron transfer and directly proportional to the logarithm of prometryn concentration (0.10–500.0 μg/L) with a correlation coefficient of 0.9960. Limits of detection in fish and seawater samples were 0.010 μg/kg and 0.050 μg/L, respectively. Excellent recoveries of 88.0%–106.0% were acquired for fish and seawater samples with a relative standard deviation below 4.2%. The constructed MIECL sensor based on MIP/CsPbBr 3 -QDs showed good stability, accuracy, and precision for sensitive detection of prometryn in aquaculture products and environmental samples.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 13
·期 1
·12
·
IF 5.2
Linwei Zhang,Xiaoyang Yin,Hongfei Yang,Hao Wen,Shiyun Han,Xiaoming Pan,Huaming Li,Dapeng Peng
Pretilachlor is a chloroacetamide herbicide mainly used for weed and broadleaf weed control in rice, that is widely utilized in China. In order to detect the residue of pretilachlor in the environment and food, a highly sensitive and specific monoclonal antibody (mAb) against pretilachlor was prepared, and the half maximum inhibitory concentration (IC50) of the monoclonal antibody was validated to be 31.47 ± 2.35 μg/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25~100 μg/L was developed. The specificity of the antibody was explained by computer simulations and experimental validation. The mAb exhibited negligible cross-reactivity towards alachlor, acetochlor, propisochlor, butachlor, and metalaxyl, and the limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.83~5.23 μg/L. The recoveries of all samples were 78.3~91.3%. The reliability of the ic-ELISA method for residue detection of pretilachlor in the environment and grains was confirmed using high performance liquid chromatography.
研究领域:
环境科学
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分析化学
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农业化学
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DOI
PubMed
高影响因子
RSC Advances
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2016
·Vol. 6
·期 20
·16790-16797
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IF 3.289
Qian Zhao,Lin Zhu
Humic acid (HA) is the main component of dissolved organic matter in aquatic ecosystems and affects the bioavailability of contaminants. Prometryn is frequently used to control annual broadleaf and grass weeds and has been widely detected in the aquatic environment. An experiment was conducted to investigate the effect of HA on the chronic toxicity of prometryn in zebrafish. In zebrafish treated with 53.2 μg L−1 prometryn (P), prometryn with 5 mg L−1 HA (P + HA5), or prometryn with 15 mg L−1 HA (P + HA15), catalase (CAT) activity and glutathione (GSH) content in the visceral mass initially increased and then decreased with exposure time. After one day of exposure, malondialdehyde in the visceral mass increased by 58% to 64% in fish treated with prometryn and HA compared to those treated with prometryn alone, however, this difference disappeared during days 10 to 40. Treatment with HA enhanced the bioaccumulation of prometryn by 34% and 40% on day 1 in the P + HA5 and P + HA15 groups, respectively, possibly due to changes in gill membrane permeability. Nevertheless, the opposite result was observed during days 10 to 40, owing to the presence of more excreta and suspended particulate matter resulting in a reduction in the quantity of free dissolved prometryn in the P + HA5 and P + HA15 groups. There were significant correlations between CAT activity and prometryn concentration (0.562**, P < 0.01) and between GSH and prometryn concentration (0.808**, P < 0.01) throughout the first 20 days of the experiment, suggesting a role for these antioxidant systems in the detoxification of prometryn in zebrafish.
研究领域:
环境毒理学
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水生生态学
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生物化学
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DOI
高影响因子
FOOD CONTROL
·
2022
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IF 6.652
Gaozhen Li,Fukai Guan,Shancang Zhao,Huihui Xu,Jiashuai Sun,Jingcheng Huang,Qingqing Yang,Yanyin Guo,Xia Sun,Yemin Guo
As an organochlorine pesticide, procymidone has become an increasing serious problem in vegetables in recent years. In order to solve a series of problems such as low sensitivity of the immunological methods, a time-resolved fluorescent immunoassay strip (TRFIAS) was developed with low limit of detection (LOD). Based on the principle of antigen-antibody immune competition, TRFIAS could detect procymidone in vegetables. After the targets being added, the samples were incubated at 37°C for 10 min. TRFIAS was observed under a UV lamp of 365 nm. The results were processed by ImageJ software to complete the qualitative and quantitative analysis of TRFIAS. Under the optimal working conditions, a linear range of TRFIAS was 5–250 ng/mL with a LOD of 0.33 ng/mL and a visual limit of detection (VDL) of 50 ng/mL. And the TRFIAS had good specificity, precision and accuracy. Comparing the results of TRFIAS with those of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), the two assays were in good agreement. Therefore, TRFIAS has the advantages of high sensitivity, high specificity, simple operation and relatively short assay time. Meanwhile, it shows absolutely great advantages in both quantitative and qualitative analysis.
研究领域:
农业化学
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分析化学
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食品科学
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产品关联
DOI
高影响因子
AQUATIC TOXICOLOGY
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2022
·
IF 5.202
Dong Wang,Guangxin Yang,Shaoguo Ru,Zhenzhong Zhang,Yuejiao Li,Jun Wang
Prometryn, a widely used triazine herbicide in agriculture and aquaculture, has been commonly detected in marine environments, but its effects on the marine copepod are unknown. In this study, marine copepod Tigriopus japonicus was chronically exposed to environmentally relevant concentrations of prometryn to investigate its impacts and potential mechanism of action. The results showed that 0.5, 5, and 50 μg/L prometryn delayed the first spawning time and hatching time, reduced the fecundity, and inhibited the population growth rate. Moreover, exposure to 0.5, 5 and 50 μg/L prometryn decreased food ingestion, the content of C and N elements, nutrient accumulation and body size, but increased the content of 20-hydroxyecdysone (20E). Transcriptome analysis showed that 50 μg/L prometryn down-regulated 1431 genes, which were mainly enriched in lysosome pathway and chitin binding and cuticle construction process. The results of qRT-PCR showed that the expression of key genes involved in juvenile hormone synthesis and chitin metabolic pathways were also inhibited after prometryn exposure. Molecular docking revealed that prometryn could bind to ecdysone receptor (EcR) and UDP-N-acetylglucosamine pyrophosphorylase (UAP), components of the ecdysteroid nuclear receptor complex. Therefore, environmental relevant prometryn delayed the molting and development of T. japonicus by disrupting the ecdysone signal pathway and chitin metabolic pathway through binding to EcR and UAP. This study provides new insights into toxic effects and molecular mechanisms of prometryn on marine copepods.
研究领域:
海洋生态学
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环境毒理学
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分子生物学
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水生毒理学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-11
·
IF 5.7
Huang Lianrun,Wang Guixiu,Wu Yanling,Wang Zhijia,Ding Yuan,Liang Hongwu,Hua Xiude
Peptidomimetic and anti-immunocomplex peptides can be easily isolated from phage display libraries, and can be used as alternatives to chemical competing haptens to improve the sensitivity of small molecule immunoassay. In this work, 16 peptidomimetics and 7 anti-immunocomplex peptides of pendimethalin (PND) were obtained from cyclic 7-, 8-, 9-, and 10-residue peptide phage libraries. Peptidomimetic EJ-2 (CMFTGTDFPC) with the highest sensitivity in competitive phage enzyme-linked immunosorbent assay (ELISA) and immunocomplex peptide EF-30 (CNPGWPPIPC) with the highest sensitivity in noncompetitive phage ELISA were selected to prepare phage-free peptides with GGGSSK-biotin at the C-terminus. Competitive and noncompetitive lateral flow immunoassays (CLFIA and NLFIA) were developed by using the phage-free peptides. After optimization, the CLFIA and NLFIA showed visual limit of detections (vLODs) of 5 ng/mL and 2.5 ng/mL, respectively, which were improved two- and fourfold compared with a LFIA based on chemical hapten. The NLFIA showed better sensitivity than CLFIA in the detection of spiked samples, and can meet the detection requirements for agro-products regulated by EU and China. The detection results of CLFIA and NLFIA for blind samples were consistent with that of ultra performance liquid chromatography/tandem mass spectrometry. Graphical abstract
研究领域:
分析化学
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免疫学
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药学
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农业科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Menghan Liu,Ruyu Zhang,Jie Wang,Huiting Zhang,Jingying Tan,Qingzhi Liu,Lei Han,Lihua Lu
Bimetallic nanozymes not only have strong synergistic catalysis ability but also may improve the catalytic specificity of the nanozymes. Herein, the oxidase (OXD)-mimic Ir/Co bimetallic nanozymes were prepared through a coordination reaction between the Ir(III) complex with the 2-methylimidazole (MeIm) ligands in ZIF-67 under mild conditions without the pyrolysis and physical doping procedures. It is found that the trace amount of Ir-modified ZIF-67 (Ir@ZIF-67) possesses 18.17-fold that of catalytic ability and 7.86-fold that of the reaction rate as those of pristine ZIF-67. On the other hand, compared with ZIF-67, the OXD-like specificity of Ir@ZIF-67 has been increased 9.7%. The theoretical simulation shows that the high-performance of Ir@ZIF-67 comes from its higher O2 adsorption ability. In virtue of the high performing OXD-like catalytic property, Ir/Co bimetallic nanozymes are employed to establish a simple and sensitive colorimetric pesticides detection platform without deleterious H2O2, which offers excellent “switch-on” malathion (MAL) detection in the linear range of 0.05–25 μM with a limit of detection (LOD) of 11.8 nM. Moreover, Ir/Co bimetallic nanozymes are successfully fabricated into the MAL test swabs. Assisted by a color-reading APP, the test swabs can detect MAL with the LOD as low as 18.5 nM. Surprisingly, such a simple test swab can achieve such high sensitivity for MAL detection. The real sample analysis verifies the high potential of the test swabs in practical application. The excellent performance of the fabricated test swabs can promote the formation of the situation where pesticides detection can be tested at any time and any place and can be carried out by anyone. This work not only provides a novel approach to designing polymetallic nanozymes but also displays the promising application of Ir/Co bimetallic nanozymes in the field of colorimetric sensing.
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xing Zhang,Beibei Liu,Rongqi Zhai,Guangyang Liu,Linglong Chen,Junjiang Liu,Maojun Jin,Jing Wang,Donghui Xu,Ge Chen
Atrazine is a selective systemic herbicide of the chlorotriazine class that is commonly used for controlling annual broadleaf and grassy weeds. However, its residue can harm the environment and human health. We have therefore developed a novel idiotypic-based noncompetitive immunoassay for the detection of atrazine by exploiting the distinctive recognition properties of anti-idiotypic antibodies. This method utilizes atrazine as a “key” to specifically dissociation the “lock” of an anti-idiotypic antibody and detection antibody immunocomplex, thereby enabling both qualitative and quantitative analysis of atrazine. Following all necessary optimizations, this method was capable exhibiting a limit of detection (LOD) of 0.52 ng/mL with R 2 = 0.990, and a linear range of 1.76 to 2604.61 ng/mL. This immunoassay offered its potential towards detection of atrazine in water and 10 kinds of vegetable samples with a recovery ranging from 70.76 to 119.28 %. The idiotypic-based noncompetitive immunoassay provides a well suitable method for low-molecular-weight contaminants immunoassay.
研究领域:
环境科学
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免疫学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Juntao Wang,Ruiyang Ma,Yaxing Guo,Fuyuan Zhang,Seid Mahdi Jafari
Accurate monitoring of nitroimidazole antibiotics (NDZs) in environmental and food matrices is critical for safeguarding public health and food safety. In this study, a novel sulfonic acid-functionalized hyper-cross-linked polymer (NDF-TPE-HCP) was synthesized via Friedel-Crafts alkylation reaction. The polymer exhibited unique mixed-mode interactions, which enabled efficient extraction of NDZs. Leveraging its strong ExEf , NDF-TPE-HCP was integrated into a solid-phase extraction system coupled with HPLC for the analysis of NDZs (metronidazole, ronidazole, secnidazole, dimetridazole and ornidazole) in water and fish muscle. The developed method demonstrated a good linearity with NDZs concentrations from 0.07 to 400 ng/g (or ng/mL) with the correlation coefficients ( R 2 ) ≥ 0.9972. Moreover, low limits of detection for the analytes (0.02–5.00 ng/g (or ng/mL)) were achieved. The method recoveries ranged from 85.2 % to 118.0 %, with the relative standard deviation (RSD) ≤ 9.8 %. The NDF-TPE-HCP based method can serve as a robust and sensitive tool for monitoring NDZs residues.
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Chen-Yan Hu,Hao Liu,Ling Xu,Yi-Li Lin,Qiang-Bing Wang,Dan-Dan Huang,Yi-Hui Wu,Zi-Yi Dong,Sheng-Jie Ji
Prometryn is one of the commonly used triazine herbicides and is also widely used for algae removal in aquaculture. This study investigated the degradation kinetics of prometryn via UV/persulfate (PS) oxidation and confirmed its adherence to pseudo-first-order kinetics, with a calculated rate constant (k obs ) of 0.2358 min −1 . The impact of PS concentration, water matrix (including background ions of Cl − and Br − and natural organic matter (NOM)), and solution pH on prometryn degradation were assessed. Results indicated an increase in prometryn degradation with raising concentrations of PS, Cl − , and Br − , especially evident at a PS concentration of 400 μm where complete degradation was achieved. Conversely, degradation decreased with increasing NOM concentration and pH. Radical scavenging experiments affirmed the promotion of prometryn degradation in UV/PS by both HO• and SO 4 − •, with SO 4 − • contributing over 70 % of the total free radical contribution. Evaluation of disinfection by-product (DBP) formation revealed trichloromethane (TCM) as the predominant DBP in non‑bromine water and tribromomethane (TBM) in bromine water. Thus, this study offers a theoretical foundation for UV/PS degradation of triazine herbicides in aqueous environments. The obtained results hold significance for controlling DBPs in subsequent water treatment processes, ensuring the provision of safe drinking water to the public and meeting the sustainable development goal 6.
研究领域:
环境科学
·
水处理工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）