INDUSTRIAL CROPS AND PRODUCTS
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2024
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IF 5.6
Hossain M. Zabed,Suely Akter,Yuehui Tian,Mudasir A. Dar,Junhua Yun,Mei Zhao,Arthur J. Ragauskas,Jia Li,Xianghui Qi
This study evaluated microbial systems and technological approaches to configure the whole slurry co-fermentation process for ethanol biosynthesis from a novel stover system rich in sugars. Two approaches, namely separate and simultaneous hydrolysis and co-fermentation (SHCF and SSCF, respectively), were investigated using Escherichia coli monoculture and E. coli- yeast coculture. The SSCF with E. coli monoculture produced 32.75 g/L ethanol, representing only 44.87% yield, which left 65.13 g/L of total sugars unconverted and exhibited limited xylose consumption. Subsequently, a coculture-based SHCF significantly enhanced sugar consumption, leading to increase in ethanol yield and concentration to 66.94% and 48.86 g/L, respectively. Nevertheless, xylose utilization remained minimal due to the preference for glucose and the inhibitory effects of certain compounds. Thereafter, modification of the medium composition by supplementing betaine and sodium metabisulfite improved ethanol production to 53.18 g/L by reducing the toxic effects of inhibitors. Finally, a dual-phase SSCF (DP-SSCF) was explored by allowing the consumption of sugars from the pretreated slurry in the first phase, followed by concurrent cellulose hydrolysis and utilization of the resulting glucose in the second phase. This strategy increased ethanol titer to 63.14 g/L, with 84.3% yield and 0.88 g/L/h productivity.
研究领域:
生物工程
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微生物学
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产品关联
DOI
高影响因子
Fermentation-Basel
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2023
·Vol. 9
·期 3
·286
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IF 3.7
Hongsen Zhang,Jiahui Jiang,Conghui Quan,Guizhong Zhao,Guotao Mao,Hui Xie,Fengqin Wang,Zhimin Wang,Jian Zhang,Pingping Zhou,Andong Song
Inhibitors from lignocellulosic biomass have become the bottleneck of biorefinery development.Gluconobacter oxydansDSM2003 showed a high performance of inhibitors degradation, which had a short lag time in non-detoxified corn stover hydrolysate and could convert 90% of aldehyde inhibitors to weaker toxic acids. In this study, an aldehyde dehydrogenase geneW826-RS0111485, which plays an important function in the conversion of aldehyde inhibitors inGluconobacter oxydansDSM2003, was identified.W826-RS0111485was found by protein profiling, then a series of enzymatic properties were determined and were heterologously expressed inE. coli. The results indicated that NADP is the most suitable cofactor of the enzyme when aldehyde inhibitor is the substrate, and it had the highest oxidation activity to furfural among several aldehyde inhibitors. Under the optimal reaction conditions (50 °C, pH 7.5), theKmandVmaxof the enzyme under furfural stress were 2.45 and 80.97, respectively, and theKcatwas 232.22 min−1. The biodetoxification performance experiments showed that the recombinantE. colicontaining the target gene completely converted 1 g/L furfural to furoic acid within 8 h, while the controlE. colionly converted 18% furfural within 8 h. It was further demonstrated thatW826-RS0111485played an important role in the detoxification of furfural. The mining of this inhibitor degradation gene could provide a theoretical basis for rational modification of industrial strains to enhance its capacity of inhibitor degradation in the future.Keywords:inhibitors degradation;Gluconobacter oxydans;protein profiling;aldehyde dehydrogenase;furfural
研究领域:
生物技术
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酶学
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微生物学
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DOI
高影响因子
Scientific Reports
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2016
·Vol. 6
·期 1
·1-11
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IF 5.228
Liu Xiumei,Xu Wenjuan,Mao Liaoyuan,Zhang Chao,Yan Peifang,Xu Zhanwei,Zhang Z. Conrad
Cellulosic ethanol production from lignocellulosic biomass offers a sustainable solution for transition from fossil based fuels to renewable alternatives. However, a few long-standing technical challenges remain to be addressed in the development of an economically viable fermentation process from lignocellulose. Such challenges include the needs to improve yeast tolerance to toxic inhibitory compounds and to achieve high fermentation efficiency with minimum detoxification steps after a simple biomass pretreatment. Here we report an in-situ detoxification strategy by PEG exo-protection of an industrial dry yeast (starch-base). The exo-protected yeast cells displayed remarkably boosted vitality with high tolerance to toxic inhibitory compounds and with largely improved ethanol productivity from crude hydrolysate derived from a pretreated lignocellulose. The PEG chemical exo-protection makes the industrial S . cerevisiae yeast directly applicable for the production of cellulosic ethanol with substantially improved productivity and yield, without of the need to use genetically modified microorganisms.
研究领域:
生物技术
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可再生能源
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微生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2021
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IF 9.642
Kongyun Zhu,Qiutong Liu,Chao Dang,Aimin Li,Lei Zhang
Integrating hydrothermal carbonization (HTC) and anaerobic digestion for biorefinery-oriented full utilization of wet organic wastes is a promising emerging technology. The objectives of this study were to identify the potential inhibitory substances, evaluate the biomethane potential of mixed and aqueous products and explore process intensifying strategies. The results indicated that the high HTC temperature of 240 °C resulted in a significantly low methane yield of 60 ± 5 mL/g COD and a high Short chain fatty acid (SCFAs) accumulation of 4174 ± 76 mg/L. GC-MS analysis showed that the contents of inhibitory pyrazines, pyridines and ketones in aqueous fraction at 240 °C substantially increased from 13.14%, 0.4%, 0.55% at 180 °C to 23.34%, 2.89%, 5.13%, respectively. When the aqueous products obtained from 240 °C-HTC was supplemented or pretreated by carbonaceous material, the methane yields were greatly improved and increased to 1.3-fold and 1.8-fold, respectively. These finding could provide some valuable technical information for HTC based biorefinery of organic waste.
研究领域:
环境科学
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生物技术
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Ke Ma,Wei Chen,Shi-Qing Yan,Zhen-Zhen Liu,Xiao-Qi Lin,Jia-Bao Zhang,Yu Gao,Tao Wang,Jian-Gang Zhang,Yong-Jun Yang
Antimicrobial compounds from the commensal gut microbiota have gained much attention due to their multifunctionality in maintaining good health in the host and killing multidrug-resistant bacteria. Our previous study showed that Paenibacillus jilinensis YPG26 isolated from chicken intestine can antagonize multiple pathogens. Herein, we characterized a bacteriocin-like inhibitory substance, jileicin, purified from P. jilinensis YPG26. Mass spectrometry analysis revealed that jileicin was a protein consisting of 211 amino acids, which showed 88.98% identity to the SIMPL domain-containing protein. The jileicin showed a relatively broad-spectrum antibacterial ability, especially against enterococci. Additionally, the jileicin exhibited good stability after various treatments, no detectable resistance, no significant cytotoxicity, and very low levels of hemolytic activity. The mode of action against Enterococcus faecium demonstrated that jileicin could destroy cell membrane integrity, increase cell membrane permeability, and eventually lead to cell death. Furthermore, jileicin was efficient in controlling the growth of E. faecium in milk. In conclusion, jileicin, as a newly identified antibacterial agent, is expected to be a promising candidate for application in the food, pharmaceutical, and biomedical industries.
研究领域:
微生物学
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食品科学
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药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2025
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IF 6.2
Jingrui Liang,Jian Du,Xiujun Zhang,Mingxue Liu,Yamin Zhang,Nannan Chen,Wenjie Sun,Jingxuan Nie,Mengxian Liang,Pinwen Wang,Xinyu He
High-solids enzymatic hydrolysis is a key process for ensuring the economic feasibility of lignocellulose bioconversion. During enzymatic hydrolysis, the rate of cellulose conversion decreases markedly with increasing solids concentration, which is attributed to inhibition by soluble compounds. Thus, gaining insights into the inhibitory properties of these compounds is essential for reducing enzyme costs. During hydrolysis, glucose-induced inhibition accounted for more than 37 % of the total inhibitory effect after 24 h, and this inhibitory effect increased with time. The inhibitor-to-enzyme ratio was a critical factor influencing the extent of inhibition; however, cellulase was more sensitive to glucose inhibition at high concentrations. As such, glucose-induced inhibition could not be overcome by increasing enzyme loading within normal dosage ranges. Different enzyme components experienced varying degrees of inhibition, and the synergistic effects among these components were also considerably reduced. Glucose inhibition of endoglucanase II involved both competitive and noncompetitive modes, which included competitive binding of carbohydrate-binding modules to the substrate and overlapping binding sites on the catalytic channel of the catalytic domain. We propose that modifying these conflicting binding sites can reliably alleviate glucose inhibition. This study has implications for addressing severe enzyme inhibition and reducing costs in bioconversion processes at a commercial scale.
研究领域:
生物化学
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生物技术
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酶学
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可再生能源
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工业微生物学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Fen Liu,Siran Feng,Akram Ali Nasser Mansoor Al-Haimi,Shunni Zhu,Huanjun Chen,Pingzhong Feng,Zhongming Wang,Lei Qin
Identifying potent bacterial algicidal agents is essential for the development of effective, safe, and economically viable algaecides. Challenges in isolating and purifying these substances from complex secretions have impeded progress in this field. Metabolomics profiling, an efficient strategy for identifying metabolites, was pioneered in identifying bacterial algicidal substances in this study. Extracellular secretions from different generations of the algicidal bacterium Brevibacillus sp. were isolated for comprehensive analysis. Specifically, a higher algicidal efficacy was observed in the secretion from Generation 3 (G3) of Brevibacillus sp. compared to Generation 1 (G1). Subsequent metabolomics profiling comparing G3 and 1 revealed 83 significantly up-regulated metabolites, of which 9 were identified as potential algicidal candidates. Back-validation highlighted the potency of 4-acetamidobutanoic acid (4-ABC) and 8-hydroxyquinoline (8-HQL), which exhibited robust algicidal activity with 3d-EC 50 values of 6.40 mg/L and 92.90 µg/L, respectively. These substances disrupted photosynthetic activity in M. aeruginosa by ceasing electron transfer in PSⅡ, like the impact exerted by Brevibacillus sp. secretion. These findings confirmed that 4-ABC and 8-HQL were the main algicidal components derived from Brevibacillus sp.. Thus, this study presents a streamlined strategy for identifying bacterial algicidal substances and unveils two novel and highly active algicidal substances. Environmental Implication Harmful cyanobacterial blooms (HCBs) pose significant environmental problems and health effects to humans and other organisms. The increasing frequency of HCBs has emerged as a pressing global concern. Bacterial-derived algicidal substances are expected to serve as effective, safe, and economically viable algaecides against HCBs. This study presents a streamlined strategy for identifying bacterial algicidal substances and unveils two novel substances (4-ABC and 8-HQL). These two substances demonstrate remarkable algicidal activity and disrupt the photosynthetic system in M. aeruginosa . They hold potential as prospective algaecides for addressing HCBs.
研究领域:
环境科学
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微生物学
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代谢组学
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水生生态学
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DOI
高影响因子
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