CHEMOSPHERE
·
2018
·
IF 4.427
Lixiao Ni,Shiyi Rong,Guoxiu Gu,Lingling Hu,Peifang Wang,Danye Li,Feifei Yue,Na Wang,Hanqi Wu,Shiyin Li
Environment-friendly algaecides based on allelopathy have been extensively studied to control harmful algal blooms (HABs). The inhibitory effects of linoleic acid (LA) sustained-release microspheres on different cell densities of Microcystis aeruginosa ( M. aeruginosa ) at different growth phases were studied. The results showed that the growth of M. aeruginosa could be inhibited within 4 days and the constant inhibitory rate with initial algal density of 8 × 10 5 cells∙mL −1 (exponential phase) was up to 96% compared with control. The chlorophyll-a content in the treatment group had the same change trend with the algal density and declined significantly at day 20th, which suggested that the microspheres could promote the degradation of chlorophyll-a. The activities of superoxide dismutase (SOD) and catalase (CAT) increased gradually within 5 days but then declined sharply, which indicated that LA microspheres could cause oxidative damage to M. aeruginosa during the process of inhibition and reduce the activities of antioxidant enzymes. In addition, the concentration of oxygen free radical (O 2 − ) increased at day 10th and rose constantly, and the content of malodialdehyde (MDA) increased to 2.7 times as much as control at day 20th. Furthermore, the content of protein, nucleic acid and the conductivity in culture solution showed a significant rise. These results showed that algal cell membrane lipid peroxidation occurred and the membrane permeability increased, accompanied by the damage of cell membrane. To sum up, the destruction of algal cell membrane is the main mechanism of LA microspheres inhibiting algal growth.
研究领域:
环境科学
·
藻类学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Yeqing Li,Shasha Yu,Xingru Yang,Yijing Feng,Liming Dong,Yi Zhang,Lu Feng,Mahmoud Mazarji,Junting Pan
In the anaerobic digestion (AD) process, the effects of humic acid (HA) derived from different feedstocks on AD are influenced by the variations in their structural composition and oxygen-containing functional groups. Thus, clarifying the structural differences of HA obtained from different feedstocks is crucial for understanding their impact on AD. In this study, the structure of five humic acids (HAs) derived from liquid digestate, food waste, silage corn straw, lignite and commercial HA, and their effects on AD were investigated. The study found that HA from food waste had more carboxyl groups , while straw-derived HA had more phenolic hydroxyl groups. Both types of HA had higher aromaticity and humification degree and showed significant inhibition effect on AD. HA from food waste had an average methanogenic inhibition rate of 43.5 % with 1 g/L HA added. In addition, commercial HA and HA derived from lignite had similar functional group types and aromaticity, with an average methanogenic inhibition rate of about 20 %. The study revealed that HAs with more carboxyl groups exhibited greater effectiveness in inhibiting AD, thereby confirming the influence of HA structures derived from different feedstocks on AD. In conclusion, this study provides valuable insights into the mechanism of HA effect on AD and offers guidance for future research focused on enhancing AD efficiency.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Research and Technology-JMR&T
·
2024
·
IF 6.2
Xuewu Li,Chenghu Ma,Tian Shi,Hejie Yang,Chuanwei Zhang,Wei Qi,Chen Li,Rongrong Liu,Weiping He,Yuanhai Liu
Aluminum-Lithium (Al-Li) alloys widely used in aerospace manufacture are highly susceptible to corrosion, which limits their industrial applications. Herein, 1H,1H,2H,2H-perfluorodecyltriethoxysilane (PFDTES) is used as a modifier for low-surface-energy treatment of TiO 2 nanoparticles. The water-based polyurethane (PU) is made as a binder to achieve a tight bonding between modified ceramic particles and Al-Li alloy, thus resulting in the extremely-feasible air-spraying preparation of a superhydrophobic coating with strong abrasion resistance. The water contact angle (CA) of as-prepared coating achieves 160 ± 0.83°, and the rolling angle (SA) is as low as 5.95 ± 0.59°. The surface structure, organization and composition are further characterized using SEM, EDS, XPS and laser confocal microscopy. Meanwhile, the electrochemical impedance and potentiodynamic polarization tests are performed for corrosion characterization. The results show that the corrosion potential of such coating increases by 170 mV compared with substrate, the corrosion current density decreases by two orders of magnitude, and the impedance modulus increases by two orders. The corrosion inhibition rate of the as-prepared coating is 98.8%, indicating that the corrosion resistance has been significantly enhanced. In addition, the mechanical robustness and stability of the coating is evaluated and confirmed using a sandpaper rubbing test. It also possesses great self-cleaning, anti-pollution and thermostable behaviors, thereby allowing them to work in extreme manufactures. Moreover, the as-prepared coating exhibits excellent adaptability to various materials and anti-icing property. This work sheds positive insights in enlarging industrial applications of multifunctional ceramic coatings in aerospace fields.
研究领域:
材料科学
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航空航天工程
·
表面工程
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Jiani Xu,Zhongyi He,Liping Xiong,Yinglei Wu,Lei Guo,Lili Li,Renhui Zhang
A high yield of environmentally friendly N,S-codoped (N,S-CDs) and N-doping carbon points (N-CDs) carbon dots was achieved through a biochemical oxidation reaction at room temperature in this study. Acetaldehyde, sodium hydroxide, benzotriazole (BTA), and 2-mercaptobenzimidazole (MB) with a similar structure were used as raw materials. The microstructure and properties of the corrosion inhibitor for Q235 steel were evaluated by various experiments. The results demonstrated enhanced corrosion inhibition rates of the N,S-CDs compared to the N-CDs using electrochemical tests (93.83% vs 77.65%) and weight loss experiments (96.35% vs 91.65%) at 50 mg/L, respectively, compared to the blank material, indicating that N,S codoping can significantly improve the corrosion inhibition effect of carbon dots. The significant improvements were attributed to the formation of dense adsorption films and the hydrophobic properties of N and S-CDs nanoparticles on the steel surface, leading to an effective barrier against corrosion. The findings from this study provide important experimental data for potential industrial applications and hold important practical value in the field of pickling corrosion inhibitors.
研究领域:
材料科学
·
腐蚀与防护
·
工业应用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
·
2025
·
IF 7.5
Hang Ye,Qi Liu,Tong Wu,Wanzhen Xue,Chunkai Wang,Kai Cheng,Tiong Michelle,Dan Luo,Chenggang Xian,Yiqun Liu
Asphaltene deposition is a significant issue in CO 2 -EOR, leading to reservoir damage and reduced efficiency. In this study, oil-soluble carbon quantum dots (CQDs) were synthesized to address asphaltene deposition issues, evaluating the inhibitory performance through laboratory experiments and molecular dynamics (MD) simulations. Experimental results demonstrated that CQDs effectively delayed the onset of asphaltene deposition from 45 vol% to 55 vol%, reduced the average particle size of asphaltene aggregates from 1200 nm to approximately 600 nm, and achieved an inhibition rate of 52.7 %. MD simulations revealed that CQDs reduced the mobility of asphaltene molecules and decreased the number of stacked asphaltene model molecules in aggregates from four to two, thereby lowering the risk of aggregation and subsequent deposition. Furthermore, the study showed that CQDs primarily inhibit asphaltene aggregation and deposition by weakening T-shaped or offset stacking interactions between asphaltene molecules. This effect was attributed to the strong interactions between CQDs and asphaltenes, with stabilization energies reaching up to −90 kcal/mol. Asphaltene molecules formed π-π interactions with CQDs through π-electron cloud overlap, dispersing the π-π interactions originally occurring between asphaltene molecules. This ultimately suppressed asphaltene aggregation. These characteristics suggested that CQDs offer a promising new approach to mitigate asphaltene deposition and have the potential to enhance oil recovery in low-permeability reservoirs.
研究领域:
石油工程
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材料科学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2009
·Vol. 20
·期 32
·325102
·
IF 3.446
Jian Zhang,Zhiyu Qian,Yueqing Gu
Thermally responsive poly(N-isopropylacrylamide-co-acrylamide) (P(NIPA-co-AAm)) nanohydrogel (NHG) with a
diameter of about 50 nm and a lower critical solution temperature (LCST) of about
40 °C
was synthesized by a previously reported precipitation polymerization method. The
physical properties including LCST, diameter and morphology were characterized. Four
hydrophobic model drugs (5-fluorouracil (5-FU), fluorescein, docetaxel (DTX) and
near-infrared dye-12 (NIRD-12)) with different hydrophilicities were respectively entrapped
into the nanoparticles and their in vitro release kinetics from NHG was investigated. DTX
was ultimately chosen as the goal anti-tumor drug and optimally entrapped into NHG with
a drug loading content (DLC) of 7.38% and encapsulation efficiency (EE) of 73.8%. An
in vitro drug release test indicated that DTX-loaded NHG had zero-order release kinetics at
43 °C. The respective anti-tumor efficacy of DTX-loaded NHG with or without hyperthermia on
tumor tissue was evaluated in Kunming mice-bearing S180 sarcoma. The inhibition rates of
DTX-loaded NHG with or without hyperthermia were 78.15% and 48.78%, respectively.
DTX-loaded NHG also showed much lower toxicity during the therapeutic procedure.
Results indicated that this kind of thermally responsive, drug-loaded NHG could be used as
a promising strategy for tumor therapy with the help of local hyperthermia treatment.
研究领域:
药剂学
·
肿瘤学
·
生物材料学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Huihui Li,Kang Liu,Zexin Yang,Quanwei Sun,Wencui Shang,Yunlong Li,Mingjing Wang,Ye Yang,Hanmeng Liu,Dengke Yin,Wei Shen
Although vascular disrupting agents (VDAs) can induce shutdown of blood flow and necrosis in the tumor core, eradicating tumor rim cells remains a significant challenge. Recently, researchers have developed various combination treatment strategies to improve the efficacy of VDAs. However, the aggravated hypoxic tumor microenvironment following vascular disruption limits the effectiveness of conventional therapeutic approaches. Here, we developed an ε-polylysine-derived oncolytic polymer (named OPAA) with membrane lytic activity. Its cytotoxic effect on tumor cells is largely unaffected by hypoxic conditions, as evidenced by the ratio of its IC50 value for 4 T1 cells under normoxic conditions to that under hypoxic conditions, which is 0.98. Subsequently, a pH-responsive combretastatin A4 phosphate disodium salt (CA4P)-loaded nanoparticle (OPAA@CA4P NPs) has been designed to efficiently deliver OPAA and CA4P to solid tumors. OPAA@CA4P NPs exhibited a prolonged serum half-life (t 1/2 = 3.15 h) compared to CA4P (t 1/2 = 0.31 h) and an enhanced tumor accumulation. In addition, CA4P can be responsively released within the tumor microenvironment, leading to necrosis in the tumor center. Concurrently, OPAA released from the nanoparticles eradicated the surviving cancer cells at the tumor periphery, thereby improving the overall therapeutic effect. Notably, compared to the CA4P + doxorubicin group (tumor suppression rates, TSR = 36.17 %), the OPAA@CA4P NPs group demonstrated superior therapeutic outcomes (TSR = 60.30 %). Overall, the introduction of oncolytic polymers provides new insights into the potential future applications of VDAs.
研究领域:
药理学
·
肿瘤学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2020
·Vol. 58
·期 10
·3780-3789
·
IF 1.946
Chen Di,Shu Yang,Chen Jian,Cao Xianying
This work proposes a novel potential source of antiallergens based on bioactive peptides. Cashew-nut protein hydrolysate with antiallergic activity was prepared from cashew nuts through protease treatment. The change in the antiallergic activity of cashew-nut protein hydrolysate during in vitro simulated digestion was investigated. Cashew-nut protein hydrolysates were prepared through treatment using five different enzymes, namely, Alcalase, Protamex, Neutrase, papain, and bromelin. According to the results of molecular weight distribution, more small molecular weight peptides could be obtained by selecting Alcalase protease than other proteases, and the degree of hydrolysis, trichloroacetic acid-soluble peptide yield and hyaluronidase inhibitory rate of the hydrolysate were 17.0 ± 61.52%, 26.28 ± 0.13% and 62.06% ± 5.07%, which were significantly higher than those of other proteases. Therefore, Alcalase is the most suitable protease for the preparation of cashew-nut hydrolysates. Cashew-nut protein hydrolysates prepared with Alcalase under optimum conditions were fractionated through ultrafiltration. Fractions with low molecular weight exhibited the highest hyaluronidase inhibitory rate (90.57%) among all fractions. The inhibition of hyaluronidase activity during digestion showed that cashew-nut protein hydrolysate III (CPH III) has persistent antiallergic activity. Therefore, CPH III could serve as a potential source of functional peptides with health-promoting effects.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
·
2019
·Vol. 96
·期 1
·169-179
·
IF 1.429
Ma Mingfang,Shang Wenqing,Jia Ruxiao,Chen Ruijiao,Zhao Min,Wang Chaoqun,Tian Mingyan,Yang Shulei,Hao Aiyou
Abstract Folic acid, as a significant vitamin, is essential for human bodies, especially for pregnant women. However, folic acid hydrogel for drug delivery has not been reported until now. Herein, folic acid hydrogel was prepared by ion inducing method firstly. Then β-cyclodextrin/camptothecin inclusion complex was loaded into folic acid hydrogel successfully by co-assembly. Camptothecin loading folic acid hydrogel has good rheological mechanical property and thermodynamic stability. Moreover, the possible mechanism of hydrogel formation was proposed by energy dispersive spectrometer and small-angle X-ray scattering. Besides, compared to camptothecin itself, camptothecin release rate from camptothecin loaded hydrogel was prolongated remarkably through dialysis experiment. Finally, the cell inhibitory rate of camptothecin loading folic acid hydrogel was increased observably compared to camptothecin itself. Hence, as a green drug delivery system, our folic acid hydrogel has important potential application in medical engineering material. Graphic Abstract Herein, a novel β-cyclodextrin/camptothecin inclusion complex loaded folic acid hydrogel was constructed by elaborate design and preparation, which can load camptothecin into folic acid hydrogel effectively. Moreover, compared to camptothecin itself, camptothecin release rate from camptothecin loaded hydrogel was prolongated remarkably through dialysis experiment. Finally, the cell inhibitory rate of camptothecin loading folic acid hydrogel was increased observably compared to camptothecin itself. Hence, as a green drug delivery system, our folic acid hydrogel has important potential application in medical engineering material.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Hua-jin Zeng,Li Sun,Si-meng Liu,Ling-bo Qu,Ran Yang
In recent years, seeking and screening of compounds that can inhibit and/or depolymerize protein aggregation has been a hot issue in the field of pharmaceutical research. As a new material, quantum dots have been widely concerned by medical researchers. In present study, a novel 4-mercaptophenylboronic acid functionalized MoS 2 quantum dots (4-MPBA-MoS 2 QDs) was successfully synthesized through a one-pot hydrothermal approach by using molybdate dehydrate and 4-mercaptophenylboronic acid as Mo and S source, respectively. Transmission electron microscopy observation showed that the morphology and microstructure of the 4-MPBA-MoS 2 QDs displayed uniform spherical shape with a diameter of approximately 1.5 ∼ 3.0 nm. The UV and fluorescence spectra experiments indicated that the prepared QDs had good water solubility and two weak absorption peaks were appeared at 280 nm and 310 nm. When the excitation wavelength was set to 310 nm, the 4-MPBA-MoS 2 QDs had the strongest fluorescence intensity, and the maximum emission wavelength appeared at 405 nm. In vitro experiments showed that the 4-MPBA-MoS 2 QDs could significantly reduce the aggregation of bovine serum albumin (BSA). Especially when the mass ratio of BSA to 4-MPBA-MoS 2 QDs was 1:5, the inhibition rate could reach 76.4%. Cell experiment showed that the presence of 4-MPBA-MoS 2 QDs could obviously decrease the cytotoxicity induced by BSA amyloid fibrils. Moreover, the depolymerization of BSA amyloid fibrils by 4-MPBA-MoS 2 QDs and its excellent cell permeability were also observed. Molecular docking studies have shown that 4-MPBA-MoS 2 QDs may stabilize the BSA structure through van der Waals forces, hydrophobic force, electrostatic interactions and hydrogen bonds formed between the outer layer of 4-MPBA and BSA to prevent fibrosis aggregation. The results of this study suggested that 4-MPBA-MoS 2 QDs showed low cytotoxicity, good biocompatibility and solubility, and had a great potential in the design of new drugs for the treatment of amyloid-related diseases.
研究领域:
药学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qi Sun,Guangqing Mu,Peng Sun,Qing Zhao,Xiaomeng Wu,Fanhua Kong
Casein (CN) is the primary factor leading to allergic reactions in infants to bovine milk, severely limiting the intake of bovine milk formula by allergic infants. This study investigates the conformational changes and regulation of linear epitopes during the hydrolysis of CN by five proteases derived from microbial and plant sources using multiple spectroscopic techniques combined with LC/MS-MS. The results showed that the IgE inhibition rates for SD (subtilisin hydrolysate), PD (papain hydrolysate), BD (bromelain hydrolysate), KD (proteinase K hydrolysate), and FD (ficin hydrolysate) were 43.19%, 41.99%, 35.45%, 48.15%, and 14.80%, respectively. The differences in antigenic residues remaining after hydrolysis by different proteases mainly result from the varying degrees of structural alteration of CN and the hydrolysis allergic epitope regions. This study provides theoretical basis for understanding the mechanism by which proteases reduce the allergenicity of CN. It also offers new insights into the development of multienzyme combinatorial hydrolysis strategies.
研究领域:
食品科学
·
免疫学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD QUALITY
·
2020
·
IF 1.763
Ruichang Gao,Yang Shen,Wanghui Shu,Fan Bai,Wengang Jin,Jinlin Wang,Li Yuan
The objective of this study was to investigate the effects of different enzymolysis conditions on the NO inhibition rate and DH (degree of hydrolysis) of sturgeon hydrolysates (SH) prepared by Alcalase. The NO inhibition rate of 60.23% was attained under the optimum enzymolysis conditions as follows: pH 9.0, enzymolysis time of 4.92 h, enzymolysis temperature of 55°C, solid/liquid ratio of 1 : 20, and enzyme additive amount of 7674.22 U/g protein, which was well matched with the predicted value 61.44% of the Box–Behnken design model. After the ultrafiltration of SH, SH-3 (SH < 3 kDa) could significantly decrease the levels of NO and proinflammatory cytokine level IL-6. Also, we found that the obtained SH-3 contained good properties of emulsification and possessed good WHC and OHC. SH-3 demonstrated appreciable antioxidant potential on DPPH and ABTS radical scavenging activities. These results suggested that SH-3 derived from sturgeon muscles could potentially be used as a promising ingredient against inflammatory and oxidative stress-associated diseases.
研究领域:
食品科学
·
生物化学
·
药理学
·
营养学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）