Frontiers in Pharmacology
·
2022
·
IF 5.988
Xiao Man,Chen Bocen,Niu Kun,Long Ziyu,Yang Fan,Xie Yiqiang
Background: Alpiniae Oxyphylla Fructus (AOF) is Traditional Chinese medicine and a dietary supplements for centuries, which posseses cardiotonic, neuroprotective, antioxidant, warming the kidney and nourish the spleen, these biological fuction is related to potential anti-aging properties. However, little is known about their effects on aging. This work aimed to investigate the effects of extracts of AOF on longevity and stress resistance in Caenorhabditis elegans (C. elegans) and the mechanisms that underlie its effects.Methods: Wild-type (WT) strand of C.elegans (N2)worms were cultured in growth medium with or without AOF. First, we examined the effects of AOF on lifespan, reproduction and healthspan assay, stress resistance and oxidative analysis, lipofuscin levels. Second, The levels of ROS and MDA, the antioxidant enzyme activities were examined to explore the underlying mechanism of AOF. Finally, the expression of the longevity-related genes were investigated to further understand the AOF’s underlying mechanism.Results: The lifespan of C. elegans was prolonged by 23.44% after treatment with high-dose AOF (100 ug/ml). AOF alleviated aging-related declines in C. elegans health and enhanced resistance to heat shock. Furthermore, AOF decreased reactive oxygen species and malondialdehyde, increased the activities of superoxide dismutase and catalase, and reduced accumulation of fat. AOF upregulated the expression of sod-3, gst-4, daf-16, and skn-1 but downregulated the expression of daf-2 and age-1 and accelerated the translocation of DAF-16 into the nucleus. The extended lifespan induced by AOF was reversed in daf-16(mu86) and skn-1(zu135) mutants, indicating that this gene is involved in AOF-regulated longevity.Conclusion: Our findings demonstrated that AOF extends lifespan and healthspan and enhances stress via boosting the activity of the antioxidant enzyme and controlling the expression of genes associated with insulin/IGF signaling and SKN-1 pathways. As a result, this work suggested AOF as a possible candidate to reduce the signs of aging by activating and inhibiting target genes.
研究领域:
老年学
·
分子生物学
·
中医药学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Xiaodong Wang,Shuang Wang,Zhipeng Yong,Geng Liu,Yinghe Cui,Dan Liang,Di Wang,Fengxiang Liu,Zhe Wang
Hydrogel electrolytes have gained significant attention in the field of flexible supercapacitors (SCs) for their intrinsic safety, high flexibility, and superior ionic conductivity . However, the water-rich structures of traditional hydrogel electrolytes inevitably cause them to freeze at subfreezing temperatures, which limits the application of flexible supercapacitors at extremely cold temperatures. Here, a compressible and low-temperature resistant supercapacitor is fabricated containing multiple hydrogen bonds cross-linked N-(2-Hydroxyethyl) acrylamide (NHEA), acrylic acid (AA), ethylene glycol/water (EG/H 2 O), and H 2 SO 4 organic gel electrolyte (OGE). The fabricated OGE exhibits high compressive stress (200 kPa), excellent shape recovery property, and high proton conductivity (62 mS/cm) even at −40 °C. Meanwhile, a reasonable assembly method of All-in-One SCs greatly reduces the contact resistance between the electrode and the gel electrolyte, progressing great capacitance (114 F/g) and flexibility. Remarkably, the device retains high capacitance retention under strong compressive stress (nearly 100%) and long-term circle (90.73% capacitance retention rate after 10,000 cycles). Furthermore, it retains near 76% capacitance at −40 °C, demonstrating the potential for applications in cold climates and high-altitude regions.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
FIBERS AND POLYMERS
·
2019
·Vol. 20
·期 2
·440-449
·
IF 1.439
Li Hongbin,Shi Wenying,Mei Shuo,Li Jinchao,Du Qiyun,Qin Longwei,Zhang Haixia
Poly( p -phenylene terephthalamide) (PPTA), as the raw polymer material of Aramid fiber-1414, has high modulus and high strength which is commonly used in the reinforced composites. However, the general blending often accompany the microphase incompatibility in polymer matrix. In this study, PPTA molecules were incorporated into poly(vinylidene fluoride) (PVDF) membrane matrix through in situ polycondensation of p -phenylene diamine (PPD) and terephthaloyl chloride (TPC) in PVDF solution and PPTA/PVDF molecular composite membranes were prepared via the subsequent nonsolvent induced phase separation (NIPS) process. The anti-compaction properties of resulted PPTA/PVDF molecular composite membranes were systematically investigated including the variations of bursting pressure and membrane thickness, porosity, pure water flux under different pressures. The results suggested that membrane comprehensive anticompaction properties were improved with the increasing of PPTA content in PVDF membrane matrix. Combining the variation trends of membrane anti-compaction properties with morphologies, the compaction-deformation evolution processes of two membrane pore types (sponge-like and finger-like pores) were proposed at the end.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2020
·Vol. 17
·期 5
·1572
·
IF 2.849
Shi-ming Li,Dan Liu,Yi-lin Liu,Bin Liu,Xing-huang Chen
Ultraviolet B (UV-B, 280–320 nm) radiation causes complex molecular reactions in cells, including DNA damage, oxidative stress, and apoptosis. This study designed a mixture consisting of quercetin, luteolin and lycopene and usedCaenorhabditis elegansas a model to study the resistance of these natural chemicals to UV-B. Specifically, we have confirmed that quercetin and its mixture can increase the resistance ofCaenorhabditis elegansto UV-B through lifespan test, reactive oxygen species level assay, germ cell apoptosis test, embryonic lethal test and RT-qPCR experiments. The results show that quercetin and its mixture prolonged the lifespan of UV-B-irradiatedCaenorhabditis elegansand reduced abnormal levels of reactive oxygen species, embryo death, and apoptosis induced by UV-B. The protective effect of quercetin and its mixture may be attributed to its down-regulation ofHUS-1,CEP-1,EGL-1andCED-13.Therefore, the results of this research could help the development of UV-B radiation protection agents.Keywords:Caenorhabditis elegans;UV-B;quercetin;luteolin;lycopene;apoptosis;stress resistance
研究领域:
分子生物学
·
辐射生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yicheng Ren,Quan Ma,Dong Li,Yanpei Chen,Qinyang Cheng,Zisheng Luo
Fluctuations in energy status are critical physiological factors influencing postharvest fruit quality. However, the role of autophagy, a stress-resistant biological process, in regulating postharvest fruit energy status remains unclear. In this study, we treated strawberry fruit with the autophagy inhibitor hydroxychloroquine (HCQ) to investigate the role of autophagy in maintaining energy status. The results demonstrated that HCQ treatment inhibited autophagosome formation, reducing relative autophagic activity by 73 % on day 6. HCQ treatment also reduced the energy charge and the NADH/NAD + ratio by 5 % and 61 %, respectively, on day 2. Corresponding to the decline in energy status, HCQ treatment led to a reduction in cytochrome C oxidase (CCO) and succinate dehydrogenase (SDH) activities by 47.48 % and 56.34 %, respectively, on day 3. Additionally, fluctuations in energy charge led to abnormalities in ripening and decreased stress resistance in autophagy-inhibited fruit. In conclusion, this study provides evidence that autophagy-mediated energy charge sustainability mitigates postharvest quality deterioration in fruit.
研究领域:
植物生理学
·
采后生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Jiahui Wang,Junhui Tao,Yinghui Liu,Hanqing Yu,Rui Fu,Jianbang Lu,Haodi Chou,Linhao Lan,Huazheng Sai
The application of aerogels in textiles is limited because their poor moisture permeability and mechanical properties. Aerogel flowers with down-like structures for clothing filling are prepared by laser cutting bacterial cellulose. The nanoporous structures of the aerogels maintained excellent heat preservation performance, and the large pores between flowers provided a channel for water vapor transmission. Moreover, the rigid silica coating on the nanofiber skeletons enabled them to rebound in a timely manner after being subjected to compression. The resulting vest exhibits better thermal insulation when it is 0.8 cm thinner than the down vest. This strategy should expand the potential applications of aerogel materials in the field of personal thermal management.
研究领域:
材料科学
·
纺织工程
·
纳米技术
·
热管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 10
·4471-4483
·
IF 5.396
Siqi Jiang,Na Deng,Bisheng Zheng,Tong Li,Rui Hai Liu
The effects of Rhodiola extract (RE) on longevity and stress resistance in Caenorhabditis elegans (C. elegans), and the underlying molecular mechanisms were explored in the present study. Results showed that the lifespan of C. elegans was remarkably prolonged by 37.1% after treated with high-dose RE (480 μg mL−1). Intervention with RE alleviated aging-related declines in the C. elegans model, and enhanced the stress resistance against heat shock, ultraviolet radiation and paraquat. Moreover, RE reduced the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD) and catalase (CAT). RE also upregulated the gene expression of sod-3, gst-4, daf-16, skn-1 in C. elegans, downregulated the gene expression of daf-2 and age-1, and accelerated the translocation of DAF-16 and SKN-1 into the nucleus. Furthermore, the daf-16(mu86) and skn-1(zu169) mutants reversed the extension of lifespan triggered by RE, indicating that these genes were involved in RE-regulated longevity. These results demonstrated that RE could enhance lifespan extension, healthspan and stress resistance of C. elegans via insulin/IGF signaling and SKN-1 pathways. Therefore, the present findings suggested Rhodiola as a potential candidate to ameliorate the symptoms of aging.
研究领域:
老年学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2018
·
IF 1.911
Xuebin Ma,Shuai Liu,Hejun Tang,Rong Yang,Bo Chi,Zhiwen Ye
Due to the syringeability of precursor solution and convenience of open surgical treatment, injectable hydrogels have gained growing attention in drug delivery application. For load-bearing tissue, the excellent mechanical property is an important requirement for delivery vehicles to resist external stress and loads. Herein, we prepared mechanically robust injectable hydrogels (HA/γ-PGA hydrogels for short) using methacrylate-functionalized hyaluronic acid and poly (γ-glutamic acid) via photopolymerization. The HA/γ-PGA hydrogels showed outstanding anti-compression ability and could suffer a more than 80% strain. Meanwhile, after 5 cycles of compression, HA/γ-PGA hydrogels could still recover quickly against external stress, showing excellent shape recovery capability. Moreover, the mechanical properties could be modulated easily by changing the molar ratio of HA to γ-PGA. The drug release behavior was also evaluated and the drug-loaded HA/γ-PGA hydrogels showed a weak burst release and sustained release behavior. Additionally, HA/γ-PGA hydrogels also exhibited superior biocompatibility. Therefore, HA/γ-PGA hydrogels have great potential as injectable drug carriers for load-bearing tissue application.
研究领域:
材料科学
·
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jinmin Zhao,Aqiang Chu,Juanli Chen,Pengju Qiao,Jing Fang,Zhensheng Yang,Zhongyu Duan,Hao Li
The hydrogel-based evaporator for interface evaporation provides a method for sustainable freshwater production. However, salt accumulation on the surface of the steam generator affects light absorption and reduces steam generation efficiency. Achieving a high-performance, salt-resistant steam generator remains challenging. Here, sponge-like polyelectrolyte composite hydrogel-based solar steam generator (MPS) is reported. The steam generator is designed with an interpenetrating network topology and is made by in-situ polymerizing sodium polyacrylate (P(SA)) in a sponge-like polyvinyl alcohol (PVA) hydrogel framework, using molybdenum disulfide (MoS 2 ) as a photothermal conversion material. Through the foaming process, MPS achieves self-floating and good water transportation performance, which enhances the thermal localization effect on the hydrogel surface and improves energy utilization efficiency (92.95 %), enabling efficient evaporation (3.22 kg m −2 h −1 ). Meanwhile, due to the coupling of P(SA), MPS exhibits excellent salt resistance, maintaining a long-term evaporation rate of 2.8 kg m −2 h −1 in high concentration saltwater (20 wt%). The porous structure and interconnected polymer chains enhance the mechanical performance of MPS, making it highly compressible and convenient for collecting freshwater marine and wilderness environments. The preparation strategy of MPS provides a feasible method to simultaneously improve the performance and durability of the interface steam generator in practical applications.
研究领域:
材料科学
·
环境工程
·
太阳能
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Xueyan Xu,Jingdong Yang,Xufeng Zhou,Shunqiong Jiang,Wen Chen,Zhaoping Liu
The design of high energy-power density supercapacitors requires advanced electrode materials with high surface area, suitable pore size for ion diffusion, as well as mechanical robustness under high-pressure compression. For conventional graphene materials with planar structure, high-pressure compression inevitably leads to reduced surface area and sluggish ion diffusion due to restacking. Here, we present highly crumpled nitrogen-doped graphene-like material (CNG) with abundant wrinkles in nanoscale utilizing one-step pyrolysis method to enable compression-resistant supercapacitive performance. The highly curved structure enabled CNG to retain large specific surface area (952 m 2 g −1 ), high pore volume in the range of 2–10 nm (1.3 cm 3 g −1 ) and little capacitance loss even under high pressure up to 30 MPa. The application of CNGs as electrode materials for supercapacitors displays a high energy density of 92 Wh kg −1 in ionic liquid with excellent power density and cycling stability.
研究领域:
材料科学
·
电化学
·
纳米技术
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2024
·Vol. 36
·期 33
·2407138
·
IF 27.4
Wenqi Zhu,Yuhang Zhuang,Jianqiang Weng,Qinzhui Huang,Guobin Lai,Liuyan Li,Meixin Chen,Kailai Xia,Zhixing Lu,Mingmao Wu,Zhigang Zou
MXene aerogels, known for good electrical properties, offer immense potential for the development of high-sensitivity pressure sensors. However, the intrinsic challenges stemming from the poor self-assembly capability and high hydrophilicity of MXene impede the natural drying process of MXene-based hydrogels, thereby constraining their application on a large scale in sensor technology. Herein, a graphene-assisted approach aimed at modulating the hydrophobicity and enhancing framework strength of MXene through a well-designed prefreezing technique incorporating 3D spherical macroporous structures is proposed. This synergistic strategy enables the fabrication of naturally dried MXene aerogels across various size scales. Moreover, the integration of 3D spherical macroporous structures improves elasticity and electrical responsiveness of aerogels. Consequently, the aerogel sensor exhibits great performances, including high sensitivity (1250 kPa −1 ), low detection limit (0.4 Pa), wide frequency response range (0.1–8 Hz), and excellent stability (1000 cycles). This sensor proves adept at monitoring pressure signals ranging from lightweight paper to human motion. Additionally, the application of customized laser engraving endows aerogels with unique functionalities, such as compressibility and immunity to strain, stretchability and resistance to compression, as well as wind detection. Thus, the proposed approach holds significant promise as a scalable method for the mass production of aerogels with versatile applications.
研究领域:
材料科学
·
纳米技术
·
传感技术
·
先进制造
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xia Liu,Lei Qin,Jie Yu,Wentao Sun,Jianhong Xu,Chun Li
During industrial fermentation , microbial cell factories are usually confronted with environmental or metabolic stresses, leading to the imbalance of intracellular redox and the reduction of cell metabolic capacity. Here, we constructed the genetically encoded redox biosensor system (RBS) based on redox-sensitive fluorescent proteins to detect redox metabolites , including reactive oxygen species (ROS), oxidized glutathione , NADH, and NADPH in Saccharomyces cerevisiae . The functional biosensors were quantitatively characterized and the orthogonal redox biosensor system (oRBS) was designed for detecting multiple redox metabolites. Furthermore, the compartment targeted redox biosensor system (ctRBS) was constructed to detect ROS and NADPH, revealing the distribution and spatiotemporal dynamics of ROS in yeast under various stress conditions. As a proof-of-concept, RBS was applied to evaluate the redox states of engineered yeast with stress resistance and heterogenous triterpene synthesis in vivo , elucidating the redox balance significantly affecting the growth and production phenotypes. The RBS in this study allowed the exploration of the diversity of compartmental redox state and real-time monitoring of the production process of yeast, providing a reliable and effective approach for accurate and in-depth profiling of bottlenecks of yeast cell factories.
研究领域:
生物技术
·
微生物学
·
分子生物学
·
系统生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）