INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Guozhu Ye,Minghui Li,Haining Huang,Ricardo David Avellán-Llaguno,Jinsheng Chen,Guoyou Chen,Qiansheng Huang
As emerging pollutants, antibiotic resistance genes (ARGs) and microplastics threaten the environment and human health. Gut microbiota is a hotspot for ARG emergence and spread. However, effects of microplastic exposure on the emergence and spread of gut microbial ARGs are unclear. Therefore, metagenomics was used to characterize polystyrene microplastics (PS)-induced ARG alterations in rat gut microbiota and their health risks, and to identify key ARG hosts and pathways as intervention targets. We found that PS exposure not only induced selective accumulation of glycopeptide and aminoglycoside ARGs, but also promoted mobility risks of glycopeptide and macrolide-lincosamide-streptogramin ARGs in gut microbiota. Metagenomic reassembly identified microbes belonging to Firmicutes (particularly order Clostridiales , such as speices Lachnospiraceae bacterium 3–1 and MD335) as major ARG hosts. Meanwhile, genera Enterococcus , Clostridioides and Streptococcus were main ARG hosts among human pathogens. Furthermore, glycopeptide and aminoglycoside ARGs were highly correlated with VanS/VanR signaling and its regulatory pathways of vancomycin resistance and peptidoglycan metabolism, amino sugar and nucleotide sugar metabolism, and CpxR signaling and its regulatory remodeling of cell envelope peptidoglycans and proteins in gut microbiota upon PS exposure. This study provides novel insights and intervention targets involved in PS-induced changes in gut microbial ARGs and their health risks.
研究领域:
环境科学
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微生物学
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分子生物学
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公共卫生
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yu Zhang,Dong Zhu,Jun Xie,Jiawei Xie,Chaolei Yuan,Xiaojun Shi
The vertical migration behavior of antibiotics in long-term manure-fertilized soils during rainfall remains unclear. Here, we examined antibiotics in soil profiles (0–60 cm) and leachates following each rainfall event throughout a year for three soils (acidic, neutral, and calcareous) with a 13-year history of manure application. The total concentrations of all the antibiotics in the soil profile (11.04–190.32 μg/kg) and the yearly cumulative load of these antibiotics in the leachate (4070–6900 ng/m 2 ) were significantly higher in the acidic and neutral soils than in the calcareous soil. Rainfall caused the migration of antibiotics (especially tetracyclines, quinolones, and sulfonamides) from soil to leachate, while the migration dynamics of some antibiotics differed among the three soil profiles. Random forest analysis revealed that precipitation, soil organic carbon content, pH, sand content, and the partition coefficient ( K ow ) of antibiotics were the key factors influencing the migration of antibiotics. Environmental risk assessment suggested that antibiotics in leachates pose low toxicity risks to aquatic organisms. Nevertheless, the vertical migration of some antibiotics (e.g., sulfachaloropyridazine and sufamerazine) was positively correlated with the dissemination of high-risk antibiotic resistance genes in leachates. This study advances our understanding of the precipitation-caused vertical migration of antibiotics in soil exposed to manure application, considering future increases in severe rainfall events.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2024
·Vol. 196
·期 10
·1-16
·
IF 2.9
Xu Xiaojian,Yang Chao,Zou Li,Leng Jun,Wang Ning,Zhang Jing
This work examined the occurrence characteristics and ecological risks of 31 antibiotics across five classes and seven ARGs in the surface waters of Gaoyou Lake. A total of 27 antibiotics, spanning four classes, were detected in the surface waters of Gaoyou Lake, with an overall concentration ranging from 57.5 to 114 ng/L and an average of 78.8 ng/L. Sulfonamide antibiotics exhibited the highest average concentration at 32.7 ng/L. Spatial analysis revealed that antibiotic concentration levels in the western region of the lake were higher than those in other areas. Similarly, ARGs were most abundant in this area, with sulfonamide ARGs demonstrating a notably higher mean abundance than other ARGs. Correlation analysis revealed strong positive associations between sul1 and several antibiotics, including sulfadimidine, sulfamethoxazole, ciprofloxacin, lincomycin, clindamycin, erythromycin, and intl1 ( P < 0.05), with intra-group correlations among sulfonamide ARGs exceeding those between different ARG groups. Ecological risk assessment indicated that erythromycin and sulfamethoxazole presented medium risks, whereas roxithromycin, azithromycin, and lincomycin were associated with low risks to aquatic organisms. The ecological risk proportions across monitoring sites were primarily low (10.6%) and moderate (16.7%), with no high-risk areas identified and 72.7% presenting no risk. The cumulative ecological risk quotient (RQ cum ) suggested a medium-risk level at all surveyed sites.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Shanshan Wang,Nana Xue,Wenfeng Li,Daoyong Zhang,Xiangliang Pan,Yongming Luo
The partition of antibiotics and antibiotic resistant genes (ARGs) between the microplastics (MPs) and the surrounding water with various salinity are still unclear. In this study, we hypothesized that adsorption of antibiotics on MPs might cause a significant change of the structure of microbial communities, diversity and abundance of ARGs on MPs and this might be further affected by change of salinity. In this study, we investigated adsorption of four common antibiotics (sulfamerazine, tetracycline, chloramphenicol and tylosin) to polyethylene (PE) MPs in river, estuary and marine waters, and the differences of antibiotic resistant genes (ARGs) and bacterial communities on MPs and in the three waters. The results showed that MPs can enrich antibiotics, ARGs and microbes from the surrounding water. Elevated salinity could reduce adsorption of antibiotics to MPs and the abundance of ARGs. For example, MPs can concentrate more antibiotics and ARGs in the fresh river water than in the estuary and the marine waters. In addition, ARGs and bacterial communities on MPs at various salinity were significantly different under the pressure of four antibiotics. On MPs, sul1, sulA/folP-01, tetA, tetC, tetX and ermE increased significantly but a few new ARGs such as sulA/folP-01 and tetA appeared. The structure of the bacterial communities on MPs was different from the surrounding water since some bacteria species found on MPs were barely detected in the surrounding water while some genera on MPs vanished after exposure to antibiotics. As the antibiotics adsorbed and the ARGs on MPs decreased with the water salinity, the structure of the communities on MPs thus varied with salinity change. These findings are important to understand the effects of MPs on the transport, fate and ecological risk of antibiotics and ARGs in different aquatic environments.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2020
·
IF 6.785
Shaoyu Lu,Jingjing Du,Zhenli Sun,Chuanyong Jing
Antibiotic resistance genes (ARGs) have become emerging environmental contaminants, and the effective on-site detection of ARGs is urgently needed. Herein, we constructed a hairpin-structured magnetic sensor for the analysis of a widespread ARG, tetA, using surface-enhanced Raman scattering (SERS). The SERS sensor was assembled by immobilizing core-satellite structured Fe3O4@SiO2-Au with single-stranded DNA in a folded hairpin structure. The SERS sensor exhibited good sensitivity and specificity for the detection of laboratory-synthesized tetA ssDNA fragments. In addition, this SERS strategy is the first of its kind to be employed for monitoring environmental samples in the field, with a limit of detection reaching as low as 25 copies μL–1. Univariate and multivariate linear regression equations verify the practicability of the SERS sensor for quantitative tetA determination, showing the prospect for an amplification-free alternative platform for sensitive and reliable on-site detection of ARGs in the environment.
研究领域:
环境科学
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Longji Zhu,Ruilong Li,Kai Yang,Fei Xu,Chenshuo Lin,Qinglin Chen,Dong Zhu,Qian Sun,Yong-Guan Zhu,Li Cui
Microplastics (MPs) ubiquitous in environments promote the dissemination of antibiotic resistance genes (ARGs), threatening ecosystem safety and human health. However, quantitative assessments of the health risks of ARGs (HRA) in plastisphere and an in-depth exploration of their driving mechanisms are still lacking. Here, the microbiomes, ARGs, and community assembly processes of five types of MPs in an urbanizing watershed were systematically investigated. By fully considering the abundance, clinical availability, human pathogenicity, human accessibility, and mobility of 660 ARGs in plastisphere, the HRA on MPs were quantified and compared. Polyethylene had the highest HRA among the five MP types, and urbanization further increased its risk index. In addition to abiotic factors, more complex biotic factors have been shown to drive HRA in plastisphere. Specifically, dispersal limitation accounted for the increasing diversity and interaction of bacteria that determined HRA in plastisphere. Further analysis of metabolic functions indicated that a higher HRA was accompanied by decreased normal metabolic functions of plastisphere microbiota due to the higher fitness costs of ARGs. This study advances the quantitative surveillance of HRA in plastisphere and understanding of its driving mechanisms. This will be helpful for the management of both MPs and ARGs treatments for human health.
研究领域:
环境科学
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微生物学
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公共卫生
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生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2022
·
IF 11.357
Jiahuan Li,Shaojie Ren,Xiao Qiu,Shan Zhao,Rui Wang,Yunkun Wang
To combat the spread of antibiotic resistance into the environment, we should adequately manage wastewater effluent treatment to achieve simultaneous removal of antibiotics, antibiotic resistant bacteria (ARB), and antibiotic resistance genes (ARGs). Herein, we fabricate a multifunctional electroactive poly(vinylidene fluoride) ultrafiltration membrane (C/PVDF) by phase inversion on conductive carbon cloth. The membrane possesses not only excellent retention toward ARB and ARGs but also exhibits high oxidation capacity as an electrode. Notably, sulfamethoxazole degradation involving hydroxylation and hydrolysis by the anode membrane is predominant, and the degradation efficiency is up to 81.5% at +4 V. Both electro-filtration processes exhibit significant ARB inactivation, anode filtration is superior to cathode filtration. Moreover, the degradation of intracellular ARGs (iARGs) located in the genome is more efficient than those located in the plasmid, and these degradation efficiencies at −2 V are higher than +2 V. The degradation efficiencies of extracellular ARGs (eARGs) are opposite and are lower than iARGs. Compared with regular filtration, the normalized flux of electroactive ultrafiltration membrane is improved by 18.0% at −2 V, 15.9% at +2 V, and 30.4% at +4 V during treating wastewater effluent, confirming its antifouling properties and feasibility for practical application.
研究领域:
环境科学
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微生物学
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水处理工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Jingyi Zhang,Zuxin Xu,Wenhai Chu,Feng Ju,Wei Jin,Peng Li,Rong Xiao
During the COVID-19 pandemic, excessive amounts of disinfectants and their transformation products entered sewer systems worldwide, which was an extremely rare occurrence before. The stress of residual chlorine and disinfection by-products is not only likely to promote the spread of antibiotic resistance genes (ARGs), but also leads to the enrichment of chlorine-resistant bacteria that may also be resistant to antibiotics. Therefore, the potential impact of such discharge on ARG composition should be studied and the health risks should be assessed. Thus, this study combined high-throughput 16S rRNA gene amplicon sequencing and metagenomic analysis with long-term batch tests that involved two stages of stress and recovery to comprehensively evaluate the impact of residual chlorine on the microbial community and ARG compositions in sewer systems. The tests demonstrated that the disturbance of the microbial community structure by residual chlorine was reversible, but the change in ARG composition was persistent. This study found that vertical propagation and horizontal gene transfer jointly drove ARG composition succession in the biofilm, while the driving force was mainly horizontal gene transfer in the sediment. In this process, the biocide resistance gene (BRG) subtype chtR played an important role in promoting co-selection with ARGs through plasmids and integrative and conjugative elements. Moreover, it was further shown that the addition of sodium hypochlorite increased the risk of ARGs to human health, even after discontinuation of dosing, signifying that the impact was persistent. In general, this study strengthens the co-selection theory of ARGs and BRGs, and calls for improved disinfection strategies and more environmentally friendly disinfectants.
研究领域:
环境微生物学
·
公共卫生
·
分子生态学
·
水处理工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2025
·
IF 12.4
Xuan Rui,Huabo Gong,Jinwen Hu,Haiping Yuan,Yawei Wang,Lixia Yang,Nanwen Zhu
Leachate from municipal solid waste (MSW) incineration harbors a plethora of contaminants, including antibiotics and antibiotic resistance genes (ARGs). However, the understanding of such leachate is markedly scant in comparison to that of landfill leachate. In this study, the distribution and removal of 8 sulfonamides (SAs), 4 quinolones (FQs), and 4 macrolides (MLs) antibiotics in leachate from 14 MSW incineration plants in representative cities across different regions of China were investigated. In addition, potential factors affecting the contamination levels of antibiotics and ARGs in fresh leachate were evaluated. The results showed that the total concentration of target antibiotics in fresh leachate ranged from 4406.1 to 14,930.6 ng/L. Notably, the antibiotic distribution in leachate exhibited regional disparities, influenced by economic status, climatic conditions, and waste separation policies. The absolute abundance of total ARGs ranged from 1.3 × 10 7 –4.0 × 10 8 copies/mL, with the mobile genetic elements intl1 facilitates the dissemination of qnrS, sul1 and sul2. No distinct regional distribution of the ARGs was observed among different cities. Antibiotic and ARGs distributions were significantly correlated with total organic carbon, pH, ammonia nitrogen, heavy metals, and microbial communities. Moreover, SAs were identified as contributors to the proliferation and spread of corresponding ARGs. Fourteen typical "anaerobic-anoxic/aerobic-anoxic/aerobic-ultrafiltration-nanofiltration " treatment processes removed the target antibiotics effectively (76.1 %-97.0 %). Biodegradation was considered to be the dominant antibiotic removal pathway, removing 62.0 %-90.9 % of antibiotics, while sludge adsorption removed only 1.0 %-11.7 %. This research furnishes valuable insights into the fate of antibiotics in MSW incineration leachate.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Zhiqi Wang,Jingfeng Gao,Yifan Zhao,Huihui Dai,Jingxin Jia,Da Zhang
Microplastics (MPs) and pharmaceuticals are common emerging pollutants in sewage, and their coexistence may have more negative effects on the environments. This study chose tetracycline (TC), ampicillin (AMP) and triclosan (TCS) to investigate the responses of antibiotic resistance genes (ARGs) and microbial communities on different MPs (polyvinyl chloride (PVC), polyethylene (PE)) biofilms (plastisphere). The adsorption capacity of three pharmaceuticals on PVC and PE decreased in the order of AMP > TC > TCS. PE was more conducive to microbial attachment than PVC. MPs led to the increase of the total copies of ARGs and mobile genetic elements (MGEs) in the sewage. Importantly, multidrug ARGs and MGEs were enriched on plastisphere. Furthermore, the co-occurrence of TC and MPs led to higher risks of spreading ARGs and MGEs. In addition, potential pathogenic bacteria Legionella , Mycobacterium , Neisseria and Arcobacter were more abundant on plastisphere than those in sewage, and these bacteria might be the hosts for ARGs and MGEs. This study showed that plastisphere could be repositories of ARGs and MGEs in sewage and accumulated potential pathogenic bacteria.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
·
2017
·
IF 4.307
Hong-Yan Luo,Ma-Feng Liu,Ming-Shu Wang,Xin-Xin Zhao,Ren-Yong Jia,Shun Chen,Kun-Feng Sun,Qiao Yang,Ying Wu,Xiao-Yue Chen,Francis Biville,Yuan-Feng Zou,Bo Jing,An-Chun Cheng,De-Kang Zhu
The Gram-negative bacterium Riemerella anatipestifer CH-2 is resistant to lincosamides, having a lincomycin (LCM) minimum inhibitory concentration (MIC) of 128 µg/mL. The G148_1775 gene of R. anatipestifer CH-2, designated lnu (H), encodes a 260-amino acid protein with ≤41% identity to other reported lincosamide nucleotidylyltransferases. Escherichia coli Rosetta TM (DE3) containing the pBAD24- lnu (H) plasmid showed four- and two-fold increases in the MICs of LCM and clindamycin (CLI), respectively. A kinetic assay of the purified Lnu(H) enzyme for LCM and CLI showed that the protein could inactive lincosamides. Mass spectrometry analysis demonstrated that the Lnu(H) enzyme catalysed adenylylation of lincosamides. In addition, an lnu (H) gene deletion strain exhibited 512- and 32-fold decreases in LCM and CLI MICs, respectively. The wild-type level of lincosamide resistance could be restored by complementation with a shuttle plasmid carrying the lnu (H) gene. The transformant R. anatipestifer ATCC 11845 [ lnu (H)] acquired by natural transformation also exhibited high-level lincosamide resistance. Moreover, among 175 R. anatipestifer field isolates, 56 (32.0%) were positive for the lnu (H) gene by PCR. In conclusion, Lnu(H) is a novel lincosamide nucleotidylyltransferase that inactivates LCM and CLI by nucleotidylylation, thus conferring high-level lincosamide resistance to R. anatipestifer CH-2.
研究领域:
微生物学
·
分子生物学
·
抗生素抗性研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Zheng-Zhe Zhang,Hai-Yan Hu,Jia-Jia Xu,Zhi-Jian Shi,Yang-Yang Shen,Man-Ling Shi,Ren-Cun Jin
The increasing use of engineered nanoparticles (NPs) poses an emerging challenge to biological wastewater treatment. The long-term impact of CuNPs on anaerobic ammonium oxidation (anammox) process was firstly investigated in this study. The nitrogen removal capacity of anammox reactor was nearly deprived within 30 days under the stress of 5.0 mg L −1 CuNPs and the relative abundance of anammox bacteria (Ca. Kuenenia ) was decreased from 29.59% to 17.53%. Meanwhile, copper resistance genes associated with the Cus, Cop and Pco systems were enriched to eliminate excess intracellular copper. After the withdrawal of CuNPs from the influent, the nitrogen removal capacity of anammox biomass recovered completely within 70 days. Overall, anammox biomass showed susceptibility, resistance and resilience to the stress of CuNPs. Therefore, the potential impacts of ENPs on anammox-based processes should be of great concern.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）