PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Qin Peng,Xinchang Hao,Chunyue Liu,Xiuhuan Li,Xingxing Lu,Xili Liu
Florylpicoxamid, a QiI fungicide, demonstrates broad-spectrum activity against a wide range of phytopathogenic organisms belonging to the phyla Basidiomycota and Ascomycota. Nevertheless, the potential for resistance and the underlying resistance mechanisms of Corynespora cassiicola against florylpicoxamid are still not fully understood. We determined the baseline sensitivity levels of 101 C. cassiicola isolates to florylpicoxamid. The EC 50 values varied from 0.01 to 1.18 μg/mL with an average of 0.50 μg/mL. Laboratory-induced fungicide adaptation of nine wild-type isolates generated seven C. cassiicola mutants exhibiting high level of resistance to florylpicoxamid, all originating from a single parental isolate. The mutants maintained their resistance even after undergoing ten successive cultivations on a medium devoid of fungicides. No cross-resistance was detected between florylpicoxamid and pyraclostrobin, fluopyram, prochloraz, or propineb. Five of the resistant mutants showed an improved compound fitness index (CFI) relative to their parental isolate, whereas the remainder displayed either a reduced or comparable CFI. All seven of the resistant mutants displayed an A37V substitution within the CcCytb protein, which was responsible for the resistance to florylpicoxamid, as validated through molecular docking analysis. Furthermore, an allele-specific PCR (AS-PCR) method for detecting the CcCytb A37V mutation was successfully established. In summarize, the findings of this study indicate a moderate risk of C. cassiicola developing resistance to florylpicoxamid, with the A37V substitution in CcCytb playing a key role in this resistance, detectable through the use of specific AS-PCR primers.
研究领域:
植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Huimin Wang,Pingxiao Wu,Juan Liu,Shanshan Yang,Bo Ruan,Saeed Rehman,Liangting Liu,Nengwu Zhu
Cadmium (Cd) is a toxic heavy metal and its uptake by living organisms causes adverse effect, further resulting in cycle pollution of the biosphere. The specific regulatory mechanism between clays and microbes under Cd stress remains unclear. In this study, interface interactions among clays, microbes and Cd were confirmed. Comparative transcriptome was conducted to investigate how it regulated gene expression patterns of microbes ( Chryseobacterium sp. WAL2), which exposed to a series of gradient concentrations of Cd (16, 32, 64 and 128 μg mL −1 ) for 12 d in the presence and absence of clay montmorillonite (Mt) (16 g L −1 ). Cd was highly enriched by the unique interface interactions between Mt and bacteria (67.6–82.1%), leading to a more hostile environment for bacterial cells. However, Mt ultimately enhanced bacterial resistance to Cd stress by stimulating the mechanism of bacterial resistance; namely: (i) Mt increased genes expression connected with ion transport, enhancing the uptake of Cd; (ii) Mt stimulated genes expression related to efflux pump and positively regulated cellular oxidative stress (e.g., glutathione) and Cd accumulation (e.g., cysteine) processes. Further, genes expression related to intracellular metabolic processes was enforced, which supplied a driving force and accelerated electron transfer ; (iii) Mt improved genes expression involved in DNA replication and other biological processes (e.g., terpenoid backbone biosynthesis) to maintain bacterial vitality. Therefore, the study not only optimized a unique Cd resistance mechanism of Mt on Chryseobacterium sp., but also provided a novel insight for environmental mitigation of heavy metals from the perspective of molecular biology .
研究领域:
环境微生物学
·
分子生物学
·
重金属污染治理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Kuitun Liu,Cong Rao,Chongwei Li,Wenlin Jiang,Bo Hu,Jianya Su
Azadirachtin, a natural sesquiterpenoid compound, exhibits broad-spectrum insecticidal activity, yet its mechanism of action remains incompletely understood. This study aimed to elucidate its toxicological mechanisms by developing an azadirachtin-resistant Drosophila strain through continuous laboratory selection. After five years of directional screening, we successfully established the Aza-Sel strain with a resistance ratio of 86.7-fold. Genetic analysis revealed that the resistance trait is incompletely recessive and polygenic in nature. Detoxification enzyme and synergism assays demonstrated that elevated activities of glutathione-S-transferases and esterases play crucial roles in the metabolic resistance of this strain. Cross-resistance spectrum analysis showed that the Aza-Sel strain exhibited 12.4-fold and 6.7-fold cross-resistance to emamectin benzoate and dinotefuran, respectively, while displaying negative cross-resistance to chlorpyrifos and indoxacarb, with no significant cross-resistance to other tested insecticides. Notably, the resistant strain exhibited substantial fitness costs: significant developmental delays across all stages (egg, larva, pupa), prolonged developmental duration, markedly reduced fecundity and egg hatch rate, and significantly shortened male longevity. These findings indicate that the azadirachtin-resistant strain bears severe fitness costs, which may account for the slow development of resistance under laboratory selection conditions. The results confirm the significant advantages of azadirachtin in integrated pest management, particularly its negative cross-resistance characteristics with emamectin benzoate and indoxacarb, making it an ideal option for managing resistant pests.
研究领域:
昆虫学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Jiali Yang,Xu Jing,Tengfei Wang,Jianbing Di,Li Chen,Yu Wang
Previous studies reported the ability of l -glutamate (glutamate) to induce host resistance against Alternaria alternata , but the underlying mechanism is still scarce. Here, temporal alteration in antioxidant enzymes and glutamate consuming pathways were transcriptionally and enzymatically monitored in tomato fruits. The results showed that exogenous glutamate application enhanced the activities of antioxidant enzymes, including peroxidase, superoxide dismutase and catalase, in tomatoes upon inoculation with Alternaria alternata , while lower concentrations of H 2 O 2 and malondialdehyde were observed. Interestingly, glutamate led to a decline in carbon:nitrogen ratio and induction of deaminating activity of glutamate dehydrogenase, resulting in replenishment of the tricarboxylic acid cycle. Concurrently, proline dehydrogenase mediated the proline catabolism was downregulated by glutamate, accompanied by the enhanced biosynthesis of proline due to increased expression of pyrroline-5-carboxylate synthase. Together our findings favored a model whereby overactivation of antioxidant enzymes and glutamate consuming pathways, deamination of glutamate and proline biosynthesis from glutamate, played a vital role in the mechanism of glutamate-induced resistance.
研究领域:
植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2025
·Vol. 108
·期 5
·e20355
·
IF 3.8
Liang Xu,Hongfei Gao,Xin He,Min Niu,Zhiwei Dai,Haotian Ni,Lei Su,Hongjie Wang
Ceramic thermal barrier coating (TBC) materials are used to protect the superalloys from the damage of harmful high-temperature airflow and improve the efficiency of jet and gas turbine engines. However, the long-term application of TBC materials and the robustness of these materials can be destroyed by aggressive calcium-magnesium-alumina-silicate (CMAS) melt during high-temperature service. Increasing the configuration entropy of material by doping multiple principal components has become a research hotspot in the design of corrosion-resistant thermal barrier coating material and has opened an infinite space of chemical composition, structure, and material properties. In this study, high-entropy (La 0.2 Sm 0.2 Er 0.2 Y 0.2 Yb 0.2 ) 2 Ce 2 O 7 was synthesized and its CMAS corrosion behavior was investigated by experimental investigation and first-principles calculation. The effects of the increase of configurational entropy and the subsequent potential effects on the CMAS corrosion behavior of Ce-based fluorite oxides have been sufficiently investigated. By compared with control samples, the high-entropy (La 0.2 Sm 0.2 Er 0.2 Y 0.2 Yb 0.2 ) 2 Ce 2 O 7 possesses the minimum infiltration depth of CMAS melts and the denser corrosion reaction layer, indicating the best corrosion resistance. The corrosion resistance mechanism of high-entropy (La 0.2 Sm 0.2 Er 0.2 Y 0.2 Yb 0.2 ) 2 Ce 2 O 7 was studied by first-principles calculation. The greater stability and resistance to segregation of CMAS melt in the CMAS/(La 0.2 Sm 0.2 Er 0.2 Y 0.2 Yb 0.2 ) 2 Ce 2 O 7 system, the poor adsorption capacity for CMAS melt which leads to a weak infiltration ability of CMAS melt; the weakest interfacial chemical reaction at the interface indicated by the smallest value of Griffith rupture work and the least species migration of high-entropy fluorite oxide can be responsible to the enhanced corrosion resistance. Our work reveals that increasing the configuration entropy can be an effective strategy for TBC material to enhance corrosion resistance.
研究领域:
材料科学
·
计算材料学
✓ 已验证
产品关联
DOI
高影响因子
Agronomy-Basel
·
2022
·Vol. 12
·期 11
·2724
·
IF 3.949
Qian Yang,Xia Yang,Jinlei Zhu,Tian Wei,Min Lv,Yongfeng Li
A population ofEchinochloa crus-galli(L.) P. Beauv obtained from direct-seeding rice fields in Jiangxi Province, China, exhibited high resistance levels (13.5-fold) to the acetyl-CoA carboxylase (ACCase)-inhibiting herbicide cyhalofop-butyl. Compared with the susceptible (S) population, this resistant (R) population evolved a cross-resistance to aryloxyphenoxypropionates (APPs) herbicides metamifop (2.9-fold) and fenoxapro-p-ethyl (4.1-fold), cyclohexanediones (CHDs) herbicide clethodim (4.7-fold), phenyl pyrazoline (DEN) herbicide pinoxaden (6.4-fold), and evolved multiple-resistance to acetolactate synthase (ALS)-inhibiting herbicide penoxsulam (3.6-fold), and auxin mimic herbicides quinclorac (>34.7-fold) and florpyrauxifen-benzyl (2.4-fold).ACCasegene sequencing did not reveal the existence of any known mutation point conferring with herbicide resistance. In addition, three metabolic inhibitors—one glutathione—S-transferase (GST) inhibitor (NBD-Cl), and two cytochrome P450 inhibitors (malathion and PBO)—did not reverse the cyhalofop-butyl resistance. Furthermore, enhanced metabolic rates of more than 60% 24 h after treatment with the active compound cyhalofop acid was observed in R plants compared to S plants. Hence, enhanced metabolism activity endows a non-target-site resistance to cyhalofop-butyl in the R population ofE. crus-galli. Future research will be required to determine what metabolizing enzyme genes are responsible for cyhalofop-butyl resistance inE. crus-galli.Keywords:resistance mechanisms;metabolism;cyhalofop-butyl;Echinochloa crus-galli;resistance pattern;multiple-resistance
研究领域:
植物学
·
遗传学
·
农学
✓ 已验证
产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 5
·2576-2583
·
IF 4.845
Wei Deng,Mengting Yang,Yang Li,Zhiming Xia,Yongrui Chen,Shuzhong Yuan,Qian Yang
BACKGROUND Chinese sprangletop ( Leptochloa chinensis (L.) Nees) is one of main grass weeds invading Chinese rice fields. The target-site resistance (TSR) of cyhalofop-butyl have been widely reported in L. chinensis populations, but the non-target-site resistance (NTSR) mechanisms have not yet been well-characterized. This study aims to investigate the likely NTSR in a cyhalofop-butyl-resistant L. chinensis population (YZ-R), which was collected from Yangzhou city, Jiangsu Province, China. RESULTS Dose–response assays showed the YZ-R population exhibited 191.6-fold resistance to cyhalofop-butyl, compared to the susceptible population (YZ-S). This resistance is not target-site based, because no mutations in the two ACCase genes were detected in the YZ-R plants compared to the YZ-S plants, and the ACCase genes expression levels were similar in YZ-S and YZ-R plants. In addition, the cytochrome P450 inhibitor malathion and piperonyl butoxide (PBO), and glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) did not significantly reverse cyhalofop-butyl resistance in the YZ-R population. However, liquid chromatography−mass spectrometry (LC–MS) analysis indicated that the metabolic rates of cyhalofop acid in YZ-R plants was significantly faster (5 to 10- fold) than in YZ-S plants. Furthermore, the YZ-R population showed no cross-resistance to other ACCase-inhibiting herbicides. CONCLUSION These results indicated that cyhalofop-butyl resistance in the YZ-R population is due to non-target-site based enhanced herbicide metabolism. Resistance in this population is likely involved in a specific detoxification enzyme, with possible high catalytic efficiency and strong substrate specificity, therefore leading to high-level and single resistance to cyhalofop-butyl. © 2021 Society of Chemical Industry
研究领域:
植物学
·
农业科学
·
分子生物学
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Teng-Fei Ma,Hong-Xi Ma,Chong-Yang Xing,Hui-Min Fu,Yu Shen,You-Peng Chen,Xiang-Yang Wei
This study combines physiological and proteomic analyses to explore the silver nanoparticles (Ag NPs) induced stress response and resistance mechanisms in the aerobic denitrifying Enterobacter cloacae strain HNR (strain HNR). Results demonstrated concentration-dependent inhibition: at 0.30 mM Ag NPs, bacterial growth (OD 600 ) decreased to 79.7 % of the control, while nitrate removal efficiency dropped to 52.4 %, accompanied by a 6.4-fold increase in nitrite accumulation. Activities of nitrate reductase and nitrite reductase were reduced to 34.3 % and 41.6 %, respectively. Ag NPs also compromised motility, induced oxidative stress (33.11 % rise in intracellular reactive oxygen species), and disrupted membrane integrity (10.4 % lactate dehydrogenase release). Conversely, extracellular polymeric substances (EPS) production surged by 25.6 % under low Ag NPs (0.01 mM), primarily driven by polysaccharide synthesis (55.5 % increase). Proteomics revealed 1987 differentially expressed proteins (978 upregulated, 1009 downregulated). Downregulated pathways (DNA repair, ribosome biosynthesis, glycerophospholipid metabolism, ABC transporters, oxidative phosphorylation, etc.) indicated that Ag NPs disrupted bacterial genome stability, inhibit synthesis of protein, cell membrane, and interfered with material transport and energy production. In response, strain HNR upregulated the sulfur relay system, tyrosine metabolism, and pentose and glucuronate interconversions to combat oxidative stress and energy metabolism inhibition. Additionally, strain HNR increased EPS production and cell surface positive electroactivity by upregulating the starch and sucrose metabolism and the cationic antimicrobial peptide resistance pathways to counteract the adsorption and contact of Ag NPs and silver ion. These findings will guide improvements in aerobic denitrification processes in NP-polluted aquatic environments and facilitate the broader application of aerobic denitrification technologies.
研究领域:
微生物学
·
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
WEED SCIENCE
·
2024
·
IF 2.1
Yu Liu,Kerong Shi,Haitao Gao,Shaojing Yin,Liyao Dong,Zhike Feng
Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is increasingly infesting imidazolinone-tolerant (IMI-T) rice fields in China, imazamox resistance of E. crus-galli has become the major concern for weed management in IMI-T rice fields. In this study, the susceptible population JLGY-3 (S) and the suspected resistant population JHXY-2 (R) collected from IMI-T rice fields were used as research subjects. When treated with imazamox, JHXY-2 (R) population showed a high level of herbicide resistance with a resistance index (RI) of 31.2. JHXY-2 (R) was cross-resistant to all five acetolactate synthase (ALS) inhibitors from different chemical families, but sensitive to herbicides inhibiting acetyl-CoA carboxylase (ACCase). In order to understand the reason why JHXY-2 (R) was resistant to imazamox, we performed experiments to characterize potential TSR and NTSR mechanisms. A trp-574-leu amino acid mutation in ALS and low imazamox ALS sensitivity were the main mechanism underlying imazamox resistance in this JHXY-2 (R) population. There was no significant difference in ALS gene expression and ALS protein abundance between R and S populations. High-performance liquid chromatography-tandem mass spectrometry analysis showed enhanced metabolism of imazamox in JHXY-2 (R), which was in contrast to the results of pretreatment with a metabolic enzyme inhibitor. Treatments with the P450/GST inhibitors did not alter the resistance level of JHXY-2 (R) against imazamox. To further clarify the NTSR mechanism of JHXY-2 (R), transcriptome sequencing showed that there was almost no significant difference in the expression of P450 and GST metabolic enzyme genes between R and S populations, and only GST-U1 showed a significant induction in R population. In conclusion, amino acid mutation and higher enzyme activity of ALS are the main causes of imazamox resistance in JHXY-2 (R). However, given the differences in imazamox residues in the leaves of the E. crus-galli, there may still be undetectable NTSR that are causing imazamox resistance in the R population.
研究领域:
植物学
·
遗传学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PEST SCIENCE
·
2023
·Vol. 96
·期 3
·1213-1227
·
IF 4.8
Zhang Yan-Chao,Yu Zhi-Tao,Gao Yang,Ye Wen-Nan,Peng Yu-Xuan,Zhang Shuai,Gao Cong-Fen
The brown planthopper (BPH), Nilaparvata lugens (Stål), is a major insect pest of rice in Asia and is mainly controlled with insecticides. Dinotefuran has been commercialized for control of N. lugens in China since 2013; however, the characteristics and mechanisms of resistance to dinotefuran are still unclear. Here, we conducted a long-term (from 2013 to 2021) and large geographical scale (11 provinces) resistance monitoring program for dinotefuran in the BPH in China. Before 2020, field populations of N. lugens have developed moderate resistance to dinotefuran, while since 2020, most field populations of N. lugens have developed high resistance. To develop a better resistance management strategy, a dinotefuran-resistant (Din-R) strain of N. lugens was selected in the laboratory to investigate the inheritance, possible mechanisms of resistance and potential fitness costs. This Din-R strain developed resistance to imidacloprid, nitenpyram and thiamethoxam. The dinotefuran resistance was controlled by multiple genes. RNA interference (RNAi)-mediated suppression of NlCYP6ER1 , NlCYP6CS1 and NlCYP314A1 expression increased the susceptibility of N. lugens to dinotefuran. Transgenic lines of Drosophila melanogaster expressing NlCYP6ER1 or NlCYP314A1 were less susceptible to all four neonicotinoids. Furthermore, the Din-R strain had a relative fitness of 0.64 with a substantially decreased survival rate, emergence rate and fecundity. Collectively, our results showed that dinotefuran resistance was mediated by multiple CYP genes. The high expression of these genes was related to the cross-resistance of N. lugens to other neonicotinoids. This study provided valuable information for facilitating the development of neonicotinoids resistance management strategies.
研究领域:
昆虫学
·
害虫管理
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Huanlong Peng,Wenjia Xie,Da Li,Meirou Wu,Yuguang Zhang,Haixing Xu,Jian Ye,Tingjin Ye,Liang Xu,Yongmei Liang,Wei Liu
It is necessary to study the mechanism of resistance to heavy metals in microbiological processes. In this study, Ochrobactrum MT180101 was used as the microbial source of an membrane bioreactor to investigate its degradation efficiency for electroplating wastewater and the copper-resistant mechanism. Meanwhile, excitation emission matrix-parallel factor, scanning electron microscope , atomic force microscope, fourier transform infrared spectroscopy , X-ray photoelectron spectroscopy and proteome analyses were applied to explain the comprehensive mechanism of the Ochrobactrum MT180101 resisting heavy metal toxicity. The results indicated that the Ochrobactrum MT180101 resisted heavy metal toxicity with the following pathways: i) binding metal cations on cell wall surfaces, ii) generating microbial products such as protein to chelate and stabilize the metal cations, iii) bio-transporting heavy metals from the intramembrane to the outer membrane by means of intracellular transport, and iv) reducing heavy metals through enzyme-mediated biotransformation . The results ensure that Ochrobactrum MT180101 was a copper-resistant bacterium that can be used in the pretreatment or deep treatment of electroplating wastewater.
研究领域:
环境微生物学
·
废水处理工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Nature Ecology & Evolution
·
2023
·Vol. 7
·期 9
·1444-1456
·
IF 16.8
Guo Lei,Qiao Xiaomu,Haji Diler,Zhou Tianhao,Liu Zhihan,Whiteman Noah K.,Huang Jia
The molecular mechanisms of coevolution between plants and insects remain elusive. GABA receptors are targets of many neurotoxic terpenoids, which represent the most diverse array of natural products known. Over deep evolutionary time, as plant terpene synthases diversified in plants, so did plant terpenoid defence repertoires. Here we show that herbivorous insects and their predators evolved convergent amino acid changing substitutions in duplicated copies of the Resistance to dieldrin ( Rdl ) gene that encodes the GABA receptor, and that the evolution of duplicated Rdl and terpenoid-resistant GABA receptors is associated with the diversification of moths and butterflies. These same substitutions also evolved in pests exposed to synthetic insecticides that target the GABA receptor. We used in vivo genome editing in Drosophila melanogaster to evaluate the fitness effects of each putative resistance mutation and found that pleiotropy both facilitates and constrains the evolution of GABA receptor resistance. The same genetic changes that confer resistance to terpenoids across 300 Myr of insect evolution have re-evolved in response to synthetic analogues over one human lifespan.
研究领域:
分子生物学
·
进化生物学
·
昆虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）