INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2019
·Vol. 20
·期 17
·4111
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IF 4.183
Kangfeng Cai,Huaizhou Gao,Xiaojian Wu,Shuo Zhang,Zhigang Han,Xiaohui Chen,Guoping Zhang,Fanrong Zeng
In this work, the effect of drought on K+uptake in root and its translocation from root to shoot was investigated using six barley genotypes contrasting in drought tolerance. Results showed that drought conditions caused significant changes in K+uptake and translocation in a time- and genotype-specific manner, which consequently resulted in a significant difference in tissue K+contents and drought tolerance levels between the contrasting barley genotypes. The role of K+transporters and channels and plasma membrane (PM) H+-ATPase in barley’s adaptive response to drought stress was further investigated at the transcript level. The expression of genes conferring K+uptake (HvHAK1,HvHAK5,HvKUP1,HvKUP2andHvAKT1) and xylem loading (HvSKOR) in roots were all affected by drought stress in a time- and genotype-specific manner, indicating that the regulation of these K+transporters and channels is critical for root K+uptake and root to shoot K+translocation in barley under drought stress. Furthermore, the barley genotypes showed a strong correlation between H+efflux and K+influx under drought stress, which was further confirmed by the significant up-regulation ofHvHA1andHvHA2. These results suggested an important role of plasma membrane H+-ATPase activity and/or expression in regulating the activity of K+transporters and channels under drought stress. Taken together, it may be concluded that the genotypic difference in drought stress tolerance in barley is conferred by the difference in the ability to regulate K+transporters and channels in root epidermis and stele.Keywords:drought tolerance;ion flux;transmembrane K+transport;plasma membrane H+-ATPase;barley
研究领域:
植物生理学
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分子生物学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2017
·
IF 1.624
Zhifei Chen,Zhi Wang,Yungui Yang,Min Li,Bingcheng Xu
Tall fescue ( Festuca arundinacea Schreb.) is a widely used cool-season turfgrass, and its growth is mainly limited by water deficit. Abscisic acid (ABA) and brassinolide (BR) are two important stress hormones regulating plant physiological processes and growth under water deficit. To investigate effects of exogenous ABA and BR on physiological and photosynthetic performance of tall fescue under water stress conditions, ABA (10 and 20 mg L −1 ) and BR (0.4 and 0.8 mg L −1 ) were applied individually and in combination (0.4 mg L −1 BR and 10 mg L −1 ABA) under three soil water regimes [75 ± 5% FC (HW), 50 ± 5% FC (MW) and 25 ± 5% FC (LW)] in the greenhouse. Results revealed that ABA and BR application markedly decreased the relative conductivity and malondialdehyde, notably increased leaf relative water content, antioxidant enzyme activity and proline content under water stress. ABA plus BR application was equally effective in improving the activities of antioxidant enzyme as ABA at 20 mg L −1 . ABA application reduced stomatal conductance and decreased both transpiration and net photosynthetic rate ( P n ), while BR application significantly increased P n and water use efficiency (WUE) by enhancing chlorophyll content. The ABA and BR combination application showed higher P n and WUE as well as BR single application. It indicated that ABA and BR combination application increased photosynthetic capacity, and reduced the effect on photosynthetic loss caused by ABA under water stress. All these confirmed that ABA plus BR application exhibited a synergistic interaction on enhancing drought tolerance and photosynthesis of tall fescue under water stress.
研究领域:
植物生理学
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园艺学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Yuankai Zhou,Xiaohao Guo,Zhiwen Li,Wei Liu,Wanqing Yang,Yalan Zhang,Hu Du,Evgeny Abakumov,Daishe Wu,Yongdong Zhang,Jianjun Dai,Ping Zhang,Fang Yao,Xianchuan Xie
Persistent dry conditions have significantly affected agricultural productivity, while also posing a threat to food security. To alleviate the effects of aridity, superabsorbent polymers have been widely used to improve soil water retention and increase drought tolerance in the soil recently. However, most of their raw materials originate from petroleum-based materials, which raises concerns about their biodegradability and environmental effect. This study focuses on creating cow dung-based superabsorbent polymers (CDSP) with excellent swelling capacities, achieving up to 494 g/g at solubilization equilibrium due to its porous properties and excellent water affinity. After five cycles, the swelling ratio of the group with better performance remained at nearly 300 g/g. The water retention capacity of the soil amended with CDSP increased by 1–3 times compared to the original, with the duration of water retention extending from 51 h to 72–120 h. More interestingly, CDSP improved soil pore structure and plant drought resistance, extending the survival time of plant under water deficit and increasing fresh plant weight by up to 2.6 times. These findings demonstrated the CDSP as a potential soil enhancer and a solution to climate-induced drought challenge, showing their effectiveness in sustainable agricultural development and waste resource utilization.
研究领域:
农业科学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Nature Plants
·
2025
·
IF 13.6
Chen Xiuxiu,Jin Songsong,Du Hong,Liu Zebin,Hou Congcong,Tang Ling,Wang Yuanyuan,Wang Yuxi,Yang Zhaolin,Zhao Tong,Ma Jinying,Zhang Lanjun,Li Meijing,Qi Dongdong,Li Wenlong,Wang Xiaohua,Sun Runze,Moore John P.,Liu Yongxiu,Lin Jinxing,Deng Xin
Autophagy and apoplastic ascorbic acid (AsA) play important roles in plant drought tolerance. However, the trans-plasma membrane transport of AsA and its association with autophagy during drought stress are unclear. Here we report an AsA-induced autophagy pathway in plants, wherein plasma-membrane-located cytochrome b561 and DOMON domain (CYBDOM) proteins play positive roles. CYBDOM proteins from the resurrection plant Boea hygrometrica (BhDB) and Arabidopsis (AtDB1) can transport electrons across the plasma membrane using intracellular AsA as an electron donor and apoplastic monodehydroascorbic acid or Fe 3+ as electron acceptors in Xenopus laevis oocytes. Increased apoplastic AsA, autophagy and drought tolerance are observed in BhDB - and AtDB1 -overexpressing Arabidopsis compared with the wild type. CYBDOM proteins interact with respiratory burst oxidase homologue D (RbohD), which serves as the adaptor to bind autophagy related gene 8 protein (ATG8) and cargo protein for autophagic degradation. AsA increases AtDB1 protein level and its interaction with RbohD. Together, the AsA-activated CYBDOM–RbohD synergy to induce autophagy suggests a novel mechanism in plant drought and desiccation tolerance.
研究领域:
植物学
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植物生理学
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分子生物学
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应激生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Zeyu Cai,Chuanxin Ma,Yi Hao,Weili Jia,Yini Cao,Honghong Wu,Xinxin Xu,Lanfang Han,Chunyang Li,Heping Shang,Anqi Liang,Jason C. White,Baoshan Xing
Cerium dioxide nanoparticles (CeO2 NPs) have enzyme-like properties and scavenge excess ROS induced by stressors such as drought. However, the underlying molecular mechanisms by which CeO2 NPs enhance drought resistance are unknown. In this work, both foliar application and soil injection of CeO2 NPs were used to rice seedlings under a 30 day moderate drought (40% soil relative moisture). Foliar application of 4 mg of CeO2 NPs per pot reduced excess reactive oxygen species and abscisic acid (ABA) in rice leaves, thereby maintaining chloroplast structural integrity and photosynthetic output, ultimately increasing drought-stressed rice biomass by 31.3%. Genes associated with photosynthesis and ribosome activity provided the foundation by which CeO2 NPs enhanced rice drought resistance. Importantly, these genes were tightly regulated by ABA due to the large number of abscisic acid responsive elements in their promoter regions. CeO2 NPs also upregulated the expression of soluble sugar and fatty acid synthesis associated genes in drought-stressed rice, thereby contributing to osmotic balance and membrane lipid stability. These results highlight the potential of CeO2 NPs to enhance rice photosynthesis and drought-resistant biomolecule accumulation by regulating ABA-dependent responses. This work provides further evidence demonstrating nanomaterials have great potential to sustainably promote stress resistance and climate resilient crops.
研究领域:
植物学
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植物生理学
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分子生物学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Jie Wang,Yaxun Fan,Hongliang Wang,Jiaming Yin,Weiming Tan,Xue Li,Yutan Shen,Yilin Wang
Photolysis and migration seriously reduce utilization efficiency of photosensitive agrochemicals, which leads to vast losses of ecological and economic benefits. Here we develop a novel method for preparing water-based coacervate as a stabilizer for photosensitive agrochemicals by utilizing single-chain/gemini surfactants and sodium lignosulfonate (SL), a renewable and ubiquitous biopolymer abandoned by pulping industry that presents excellent intrinsic ultraviolet (UV) resistance. Coacervates contain intricate nanoscale networks and abundant bound water, resulting in highly effective encapsulation of hydrophilic and hydrophobic solutes, and entanglement with the micro- and nanostructures of a superhydrophobic surface without sacrificing its ability to resist UV degradation. These coacervates display a high encapsulation efficiency for photosensitive abscisic acid (ABA) up to 90%, and resist UV degradation for 70 times longer than that in pure water, and act as an optimal substrate for the deposition of encapsulated agrochemicals on superhydrophobic leaves after high-speed impact. As a result, the drought resistance of wheat seedlings is significantly improved after spraying ABA protected by SL-coacervates, compared with free ABA, demonstrating that agrochemicals sequestered in coacervates effectively alleviate photolysis and migration loss. Thus, using a lignin-based coacervate can be used to potentially promote productive and sustainable agriculture, by serving as a highly efficient and environmental-friendly stabilizer for photosensitive agrochemicals used in pesticide applications.
研究领域:
农业化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Li Bingyan,Song Dali,Guo Tengfei,Xu Xinpeng,Ai Chao,Zhou Wei
Plant biostimulants (PBs) are a potential strategy to improve crop growth and grain quality. In the present study, 100 mg/L trehalose, chitosan, humic acid and gamma-aminobutyric acid treatments were applied to analyze the effects of maize production and reproductive characteristics. The contents of nitrogen, phosphorus and potassium and grain quality were significantly affected by the PBs, but not yield. The seed germination rate of all PB treatments was significantly reduced, but the drought resistance of progeny seedlings was significantly improved, with humic acid having the strongest effect. Liquid chromatography mass spectrometry analysis indicated that the disruption of the tricarboxylic acid cycle, probably due to the blockage of intermediate anabolism, reduced the supply of energy and nutrients in the early stages of germination, thus inhibiting seed germination, while the increased resistance of the offspring seedlings may be due to the up-regulation of the synthesis of unsaturated fatty acids and alkaloids by humic acid treatment. This study revealed the similarity and heterogeneity of the effects of different PBs on nutrient accumulation, yield characteristics and grain quality of maize, providing guidance for the application of PBs in intensive and sustainable agricultural production.
研究领域:
农业
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植物生理学
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代谢组学
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作物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Xia Xue,Dong Jiawei,Li Aijie,Wang Yanlin,Liu Yang,Zhu Yingfang,Xu Liang,Jing Zhiyang,Wang Jing,Zou Yan,Sun Shiyong,Wang Lu,Lu Yiqing,Soeriyadi Alex,Wang Xuelu,Patrick John W.,Offler Christina E.,Zheng Meng,Song Chun-Peng,Shi Bingyang
Presence of the cell wall and the lack of streamlined pathways for cellular delivery of external agents into plants is a core challenge of plant biotechnology and crop engineering development. However, both viral and bacterial transmission have their own restrictions and the few non-heavy metal nanodelivery platforms require external forces for tissue penetration. Such dependency limits any high-throughput application considering the large plant numbers to be treated in the field or even laboratory exercises. Herein, we demonstrate Aspartic acid (Asp) decorated poly(ethylene glycol)-block-poly(2-(diisopropylamino)ethyl methacrylate) (Asp-PEG-PDPA) copolymers assembled micelles (Asp/PDPA-NP), a platform that utilises amino acid transporters (AtAAP1 and AtLHT1) as receptors for clathrin-dependent endocytosis, freely translocate to release loaded cargo into various plant tissue/cell types in a species-independent manner within ≤10 minutes through simple spray or co-culture. As proof-of-concept, abscisic acid (ABA)-loaded Asp/PDPA-NP was tested for its efficacy to confer plant drought resistance. Asp/PDPA-NP@ABA reduced the effective ABA dose down to 1 nM (one million-fold) and elicited anti-drought potency in representative eudicot (soybean) and monocot (maize) crop species. Owing to its delivery efficiency, Asp/PDPA-NP holds promise as a potent carrier for diverse chemicals and biomolecules in plant systems.
研究领域:
植物学
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纳米技术
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生物技术
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分子生物学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Xiujing Yin,Song Liu,Yukun Qin,Ronge Xing,Kecheng Li,Chunlin Yu,Xiaolin Chen,Pengcheng Li
Chitooligosaccharide grafted with β-aminobutyric acid based on the idea of bioactive molecular splicing was prepared, and the differences in drought resistance activity before and after grafting were compared. The mechanism was investigated by comparing the differences of the derivative with the Control and Drought about metabolomes . The results showed that the expected derivative was successfully synthesized, named COS-BABA, and had better drought resistance-inducing activity than the raw materials. We suggest that COS-BABA induced drought resistance through second messenger-induced activation of signaling pathways related to traumatic acid and indol-3-lactic acid, which enhanced nucleic acid metabolism to accumulate nucleotides and decreased some amino acids to facilitate protein synthesis . These proteins are regulated to strengthen photosynthesis , resulting in the promotion of carbohydrate metabolism. The accumulation of unsaturated fatty acids stabilized the cell membrane structure and prevented nonstomatal water dissipation. This study provides ideas for the development of more effective drought resistance inducers.
研究领域:
植物科学
·
农学
·
分子生物学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
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