Antioxidants
·
2021
·Vol. 10
·期 12
·2019
·
IF 6.313
Zebin Huang,Lingna Xie,Yongyu Xu,Kai Zhao,Xuetong Li,Jiaben Zhong,Yujing Lu,Xuetao Xu,Susan Goodin,Kun Zhang,Lanyue Zhang,Chunlian Li,Xi Zheng
Zingiber striolatumDiels (Z. striolatum), a widely popular vegetable in China, is famous for its medicinal and nutritional values. However, the anti-inflammatory effects of essential oil fromZ. striolatum(EOZS) remain unclear. In this study, EOZS from seven regions in China were extracted and analyzed by GC–MS. LPS-induced RAW264.7 cells and 12-O-Tetradecanoylphorbol 13-acetate (TPA)-stimulated mice were used to evaluate the anti-inflammatory effects of EOZS. Results show that 116 compounds were identified in EOZS from seven locations. Samples 2, 4 and 5 showed the best capability on DPPH radical scavenging and NO inhibition. They also significantly reduced the production of ROS, pro-inflammatory cytokines, macrophage morphological changes, migration and phagocytic capability. Transcriptomics revealed MAPK and NF-κB signaling pathways may be involved in the anti-inflammatory mechanism, and the predictions were proven by Western blotting. In TPA-induced mice, EOZS reduced the degree of ear swelling and local immune cell infiltration by blocking the activation of MAPK and NF-κB signaling pathways, which was consistent with the in vitro experimental results. Our research unveils the antioxidant capability and potential molecular mechanism of EOZS in regulating inflammatory response, and suggests the application of EOZS as a natural antioxidant and anti-inflammatory agent in the pharmaceutical and functional food industries.Keywords:Zingiber striolatumDiels;essential oil;oxidant stress;anti-inflammatory activity;MAPK;NF-κB
研究领域:
药理学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
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2015
·
IF 2.345
Hui Xiong,Yanan Zheng,Guangzhong Yang,Huixia Wang,Zhinan Mei
The phytochemical investigation of the ethanol extract from the stems of Entada phaseoloides (L.) Merr (also called “Guo Gang Long”) led to the isolation of eleven triterpene saponins ( 1 – 11 ). Their structures were elucidated by extensive spectroscopic methods including 1D- ( 1 H and 13 C) and 2D-NMR ( 1 H– 1 H COSY, HSQC, HMBC, HSQC-TOCSY and ROESY) experiments as well as ESIMS analysis and hydrolysis. These saponins comprised entagenic acid as the main aglycon , saccharide moieties at C-3 and C-28, and esterification of C-2 or C-3 hydroxyl group of the terminal β- d -glucopyranose unit with a monoterpenic acid. To further explain the clinical applications of “Guo Gang Long” for its anti-inflammatory effect, the inhibitory activities on the production of NO of the saponins and the related aglycon, entagenic acid ( 12 ), were evaluated in vitro. The compounds containing a free hydroxyl at C-3 of aglycon ( 1 and 4 ) and entagenic acid showed significant activities against NO production in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells with IC 50 values of 25.08, 20.13 and 23.48 μM, respectively. And the three compounds could also inhibit the release of pro-inflammatory cytokines, such as TNF-α, IL-1β, IL-6 and IL-8.
研究领域:
植物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2024
·
IF 1.5
Yan Lei,Bing Wang,Guanglin Zhu,Guo Feng,Wei Li,Caiyao Han,Qian Qin,Xie-an Yu,Xueli Song,Zhengyan He,Ju Zhang,Hongmei Su,Wenjing Wang
Background: In the research of the pharmacological activity of modern Chinese medicine, a large number of traditional Chinese medicines show anti-inflammatory activity. Asparagi radix is one of the anti-inflammatory drugs. With the deepening of people's research on Asparagi radix, its anti-inflammatory pharmacological effects have been gradually explored, but its specific anti-inflammatory active ingredients are still unclear. Therefore, it is necessary to screen and identify its specific anti-inflammatory active ingredients from Asparagi radix. At present, COX-2 is an important target for the treatment of chronic inflammation, and many drugs targeting this target have been marketed. However, some drugs have serious side effects, so it is very meaningful to study new COX-2 inhibitors. Methods: In this experiment, the fractions of different time periods are separated by high performance liquid chromatography-separation-trapping system (HPLC-CAD-FC). Then, ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) and biological activity analysis techniques are used to identify the chemical composition and in vitro anti-inflammatory activity of each fraction. Finally, the data is integrated to screen out the substances with potential anti-inflammatory activity in Asparagi radix. Results: The results showed that the method established in this experiment can successfully isolate the active components of Asparagi radix, and four saponins with potential anti-inflammatory activity are identified. Conclusion: In summary, the analysis method established in this study can quickly and effectively screen out its anti-inflammatory active substances, and its potential anti-inflammatory active ingredients are mainly steroidal saponins.
研究领域:
中药学
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药理学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Wenqian Luo,Zhiyun Du,Yating Zheng,Xiaoxin Liang,Guomeng Huang,Qianhua Zhang,Zexuan Liu,Kun Zhang,Xi Zheng,Li Lin,Lanyue Zhang
Essential oils isolated from the Lamiaceae species are attracting increasing interest because of their applications in natural food preservation and pharmaceutics. The aim of this study was to systematically investigate the composition and biological effects of six Lamiaceae essential oils. Approximately 106 components were identified by gas chromatography-mass spectrometry (GC–MS), and the predominant compounds were linalool (0.05–46.55%), α-pinene (0.12–45.35%), o- cymene (0.91–41.20%), patchouli alcohol (28.27%), dl -menthol (21.12%), isobornyl acetate (22.52%), d -limonene (0.01–18.42%), α-terpineol (0.07–4.88%), and β-pinene (0.08–2.03%). Most of these essential oils were found to have effective antioxidant activities (IC 50 ; 1.07–18.77%), as estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assays. The essential oils not only had antibacterial activities (6.5–12.3 mm diameter inhibition zones), but also had antifungal activities (6.5–19.0 mm diameter inhibition zones). The essential oils exhibited strong anti-inflammatory activities and markedly decreased the production of tumor necrosis factor (TNF-α), nuclear factor-kappa B (NF-κB), interleukin 6 (IL-6), and cyclooxygenase 2 (COX-2). The most abundant compounds in the essential oils isolated from the six Lamiaceae species have potential applications in the scavenging of free radicals, inhibition of bacterial and fungal growth, and relief from inflammation.
研究领域:
植物化学
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药理学
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食品科学
✓ 已验证
DOI
高影响因子
Drug Design Development and Therapy
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2019
·
IF 3.208
Yan Yan,Pengchong Wang,Ruiying Li,Ying Sun,Rui Zhang,Chuanchuan Huo,Jianfeng Xing,Yalin Dong
Purpose Budesonide (Bud) is a nonhalogenated glucocorticoid with high anti-inflammatory potency and low systemic side effects. However, the poor water solubility of Bud affects its dissolution and release behavior, thus influencing its anti-inflammatory effect. This study was aimed at synthesizing and evaluating novel conjugates of Bud, hoping to increase the anti-inflammatory activity of Bud by improving its water solubility.Materials and methods Seven novel Bud conjugates (3a–3g) were designed and synthesized in this study. Besides, the equilibrium solubility, cell viability, in vitro and in vivo anti-inflammatory activity, and the hydrolysis behavior of the conjugates in different pH solutions, rat and human plasma, and rat lung homogenate were studied in detail.Results As compared to Bud, the equilibrium solubility of 3a, 3c, and 3e was significantly increased; 3a, 3b, and 3c significantly inhibited the interleukin-6 production in lipopolysaccharide-induced A549 cells; 3a and 3e could significantly decrease the xylene-induced ear edema; and 3a and 3c were gradually and slowly hydrolyzed into Bud in the alveolar fluid and lung homogenate and broken down quickly in plasma.Conclusion The amino acid ester compounds budesonide-21-glycine ester (3a) and budesonide-21-alanine ester (3c) were selected as potential conjugates of Bud. This study would provide a theoretical and an experimental basis for the in vivo process of glucocorticoids and the treatment of inflammatory diseases.
研究领域:
药理学
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药物化学
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炎症研究
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jingsi Zhang,Xin Chen,Qiping Zhan,Yifan Wang,Keke Meng,Qiuhui Hu,Liyan Zhao
Flammulina velutipes ( F. velutipes ) polysaccharides have been shown to play a crucial role in preventing inflammatory diseases. However, further exploration is needed to understand the specific relationship between their structure and anti-inflammatory activity. In this study, four structurally distinct F. velutipes polysaccharides (FVP2, FVP2U1, FVP2U2, and FVP2U3) were isolated via a previously established ultrasonic degradation model, and the exploration on the impact of chain conformation on their anti-inflammatory activities was focused. The results indicated that FVP2 exhibited a spherical conformation, while FVP2U1 and FVP2U2 displayed flexible chains, and FVP2U3 showed a semi-rigid chain. The study on the impact of four kinds of F. velutipes polysaccharides on inflammatory response in LPS-stimulated RAW264.7 cells revealed that ultrasonic degradation enhanced the anti-inflammatory activity of FVP2. The primary anti-inflammatory mechanism involved the inhibition of NO, TNF-α, IL-1β, and IL-6 release, and reduced transcriptional levels of TLR4, MyD88, NF-κB, and NLRP3. Among the ultrasonically degraded polysaccharides with compliant chain conformations and lower molecular weight demonstrated superior anti-inflammatory activity. These results provide insights into the relationship between molecular weight, chain conformation, and anti-inflammatory activity of F. velutipes polysaccharides, contributing to the potential application of ultrasound in developing highly active anti-inflammatory polysaccharides and related nutritional products.
研究领域:
生物化学
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免疫学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Ming Li,Tongcheng Xu,Wenhao Zheng,Boyan Gao,Hongyan Zhu,Ruofei Xu,Hanyu Deng,Bo Wang,Yanbei Wu,Xiangjun Sun,Yaqiong Zhang,Liangli (Lucy) Yu
Six commercial red sorghum varieties (Tong Za 117, 141, 142 and 143, Chi Za 109 and 101) were investigated for their triacylglycerol (TAG) profiles, soluble and bound phenolics, and radical scavenging and anti-inflammatory activities. A total of 21 TAGs were identified in red sorghum oils for the first time. Total phenolic (TPC) and flavonoid contents (TFC) in the soluble or bound phenolic fractions differed among red sorghums. Significant correlation among TPC, TFC and DPPH radical scavenging activities was observed in both fractions. Except for caffeic acid, most of phenolic acids in red sorghums are in the bound form. Soluble 3-deoxyanthocyanidins contents (2.12–57.14 μg/g) were significantly higher than those of bound forms (0.01–0.18 μg/g) regardless of sorghum varieties and types of 3-deoxyanthocyanidins. Moreover, the stronger anti-inflammatory capacity of soluble phenolic fraction in Tong Za 117 correlated with its higher TPC, TFC and radical scavenging activity than those of its bound counterpart.
研究领域:
食品科学
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营养学
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植物化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Liangliang He,Huifang Duan,Xintong Chen,Yuanshan Chen,Qingmei Mo,Junqing Huang,Huinan Zhao,Xinsheng Yao,Jiaxu Chen,Zhihong Yao
Dried ginger, a well-known medicine and food homologous production, has been widely circulated in China with high health benefits and economic value. Currently, there is still a lack of quality assessment on whether dried ginger in China exhibits chemically and biologically distinct properties, which creates a barrier to its quality control in commercial circulation. In this study, the chemical characteristics of 34 batches of common dried ginger samples in China were first explored using non-targeted chemometrics based on the UPLC-Q/TOF-MS analysis, leading to the identification of 35 chemicals that contributed to clustering into two categories, with sulfonated conjugates being the key chemically distinct components. By comparing the samples before and after sulfur-containing treatment and the further synthesis of a key differentiating component of [6]-gingesulfonic acid, it was then demonstrated that sulfur-containing treatment was the primary cause of the formation of sulfonated conjugates, as opposed to regional or environmental factors. Furthermore, the anti-inflammatory efficacy of dried ginger with high presence of sulfonated conjugates was significantly decreased. Consequently, for the first time, UPLC-QqQ-MS/MS was used to develop a targeted quantification method for 10 characteristic chemicals in dried ginger, allowing researchers to quickly determine whether dried ginger has been processed with sulfur and quantitatively evaluate the quality of dried ginger. These results provided an insight into the quality of commercial dried ginger in China and a suggested method for its quality supervision as well.
研究领域:
中药学
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分析化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2018
·
IF 3.186
Xing-Long Chen,Fang Liu,Xue-Rong Xiao,Xiu-Wei Yang,Fei Li
Tripterygium hypoglaucum (H. Lév.) Hutch. has been used to remedy rheumatoid arthritis , however, it shows frequent toxicity to the body. In this study, liquid chromatograph-mass spectrometer (LC-MS) was guided to characterize abietanes diterpenoids with anti-inflammatory activity from the stem of T. hypoglaucum . Thirteen undescribed abietanes diterpenoids were isolated and purified, and their chemical structure was identified using various spectroscopic methods . These compounds belonged to abietanes with splitting C ring, abietanes with benzenoid rings, diterpene quinoids , diterpene quinoids with lactone rings, and abietanes with benzenoid and lactone rings, respectively. Lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages was used to evaluate anti-inflammatory activity of the compounds. The results indicated that hypoglicin B-G and hypoglicin J-M exhibited inhibitory activity of NO production with the IC 50 values of 6.01, 25.21, 8.29, 3.63, 0.72, 0.89, 36.91, 0.82, 2.85, 11.92 μM, respectively. Among these compounds, compound hypoglicin L showed high anti-inflammatory activity and low toxicity (SI = 5.02 × 10 4 ). Further QPCR analysis revealed that hypoglicin D and hypoglicin L can inhibit the mRNA expression of iNOS in LPS-stimulated RAW264.7 cells at doses of 12.5 and 3.13 μM, respectively. Taken together, ten anti-inflammatory diterpenoids were found from T. hypoglaucum in this study.
研究领域:
药理学
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植物化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Essential Oil Bearing Plants
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2018
·
IF 0.681
Mengxia Zhu,Lijun Ling,Ji De,Yan Lu,Daofeng Chen
The leaves and twigs of Juniperus pingii var. wilsonii (Cupressaceae) smell aromatic and are traditionally used as the Tibetan medicine Xuba. Their essential oil obtained by ultrasonic-assisted hydrodistillation and volatiles collected by GC headspace technique were analyzed and compared by GC-MS. The analyses revealed the presence of thirty-nine components in the essential oil, representing 95.8% in content of the total oil, mainly including sabinene (22.6%), elemol (15.5%) and (-)-terpinen-4-ol (9.6%). Thirty-eight components accounting for 99.4% of the headspace volatiles were identified, mainly including sabinene (32.5%), β-pinene (21.9%) and α-thujene (10.3%). The compositions of the oil and headspace volatiles were quite similar, and twenty-five compounds identified were in common, which was 91.3% of the oil and 96.0% of the headspace volatiles in content. The essential oil could obviously inhibit Nitric oxide production in LPS-stimulated RAW 264.7 cells with no significant effect on cell viability, indicating its good anti-inflammatory activity.
研究领域:
植物学
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药理学
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天然产物化学
✓ 已验证
DOI
高影响因子
AAPS PHARMSCITECH
·
2016
·Vol. 18
·期 3
·729-737
·
IF 1.954
Li Yongquan,Xiao Yu,Yin Zongning
As an effective COX-2 inhibitor, celecoxib is widely used in anti-inflammation therapy. However, it may cause cardiovascular risks and renal adverse effects. In the present study, we aimed to construct a celecoxib prodrug with enhanced anti-inflammatory efficacy and reduced adverse effects using folate in order to target activated macrophages. Folate-glycine-celecoxib was synthesized and identified by 1 H-NMR, MS, and FTIR analyses. The cytotoxicity of folate-glycine-celecoxib was tested on murine macrophage cells (RAW264.7) using thiazolyl blue tetrazolium bromide. Cellular uptake studies were employed to determine targeting ability toward folate receptors via flow cytometry and confocal microscopy. Anti-inflammatory efficacy of folate-glycine-celecoxib was investigated by measuring the concentration of LPS-induced nitric oxide (NO). Folate-glycine-celecoxib exhibited lower cytotoxicity than conventional celecoxib, and this conjugate could be targetedly transported into RAW264.7 cells through binding with folate receptors on cell surface. Through targeting to RAW264.7 cells, folate-glycine-celecoxib exhibited better effects than equimolar celecoxib in NO inhibition, suggesting greater anti-inflammatory activity. These findings demonstrated that the prodrug folate-glycine-celecoxib had potential to treat inflammatory disease with low cytotoxicity and high targeting ability.
研究领域:
药理学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Lin Li-Bin,Jian Xiao,Qiang Zhang,Rui Han,Biao Xu,Sheng-Xiang Yang,Wen-Bo Han,Jiang-Jiang Tang,Jin-Ming Gao
Fourteen eremophilane sesquiterpenoids (1–14), including nine new congeners, septoreremophilanes A–I (1–9), together with three known sesquiterpenes (15–17), two known tetralone derivatives (18, 19), and two known cholesterol analogues (20, 21), were isolated from the endophytic fungus Septoria rudbeckiae. Compounds 1–6 and 7a belong to the family of the highly oxygenated eremophilane sesquiterpenoids with a 6/6/5 tricyclic system and bearing a hemiacetal moiety. The inhibitions of all metabolites against eight bacteria were estimated in vitro, and nine new metabolites (1–9) were tested for antineuroinflammatory activity. Notably, the effects of 4 against Pseudomonas syringae pv. actinidae and 20 against Bacillus cereus displayed potent inhibitory, with the MIC values of 6.25 and 6.25 μM, respectively. Further, scanning electron microscopy analyses indicated that 4 and 20 were to change the outer configuration of bacterial cells, respectively, and the investigations demonstrated that 4 and 20 may act as potential structure templates for the development of the agrochemical bactericides. Additionally, compound 6 displayed potent inhibition of NO generation in lipopolysaccharide-induced BV-2 microglial cells (IC50 = 12.0 ± 0.32 μM), and the conceivable anti-inflammatory mechanisms implicated were also investigated by molecular docking. Thus, the bioactive metabolites of the strain S. rudbeckiae may serve as a novel resource to be developed.
研究领域:
微生物学
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天然产物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）