JOURNAL OF MEMBRANE SCIENCE
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2024
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IF 8.4
Nan Ma,Guiliang Li,Yang Liu,Jintao Yang,Yingyao He,Fu Liu
Nanofiltration membrane technology for antibiotic desalination in fermentation broth represents a low-energy and high-purity approach. However, it is constrained by the low perm-selectivity and susceptibility to membrane fouling. Here, we develop a novel zwitterionic nanofiltration membrane by incorporating an N-Oxide directly connected zwitterion N,N-bis(3-aminopropyl)methylamine N-oxide (DNMAO) into the nascent polyamide layer via interfacial polymerization. The N-Oxide derived zwitterion created a looser crosslinking network in addition to the trimesoyl chloride-piperazine (TMC-PIP) network, which was supported by Stokes radius related with molecular weight cut-off measurement, and Brunauer-Emmett-Teller Ar adsorption isotherms. Moreover, the directly connected groups (N + -O - ) without spacers endow excellent hydration ability and mitigate membrane fouling. The optimized membrane exhibits a water flux of 114.9 L m −2 h −1 and exceptional anti-fouling performance with a flux recovery ratio as high as about 96.4 %. Furthermore, the salt/antibiotic separation factor maintained at 41.1 when continuously filtrating NaCl/erythromycin (ERY) mixed solution for 7 h, which showed a great potential in antibiotic purification.
研究领域:
环境科学
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材料科学
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生物工程
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DOI
高影响因子
Journal of Environmental Sciences
·
2025
·
IF 6.3
Xuefei Wu,Hualong Sun,Gang Wang,Li Liu,Mengqi Zheng,Guowei Chen
Cyanobacteria are competent hosts for antibiotic resistance genes, influencing the spread of bacterial resistance through their complex interactions within aquatic environments. However, the coevolution of antibiotic resistance between cyanobacteria and bacteria under sub-inhibitory antibiotic stresses remains unclear. To bridge this knowledge gap, we investigated the effects of Microcystis aeruginosa on modulating bacterial resistance evolution in aquatic ecosystems exposed to sub-inhibitory antibiotics. Results show that cyanobacteria reduced the abundance of antibiotic resistance bacteria (ARB) by 62% to 93% in antibiotic-free conditions and by up to 80 % under sub-inhibitory antibiotic concentrations. The concurrence of cyanobacteria and antibiotics significantly alter microbial community compositions, with Proteobacteria emerging as the dominant population (51%–66%) and Bacteroidota proliferating by 8.7-fold, alongside a significant enrichment of metabolism-related and pathogenic genes (p < 0.05). Redundancy analysis revealed that ARB prevalence was positively correlated with the abundances of Proteobacteria and Bacteroidota , but negatively correlated with Cyanobacteria . Our findings suggest that cyanobacteria may reduce the spread of bacterial resistance both in antibiotic-free and sub-inhibitory antibiotic environments, by reshaping population interactions. These insights are crucial for evaluating the risk of antibiotic-driven bacterial resistance spread in cyanobacteria-rich environments and are vital for the protection and assessment of aquatic environmental quality.
研究领域:
环境微生物学
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抗生素抗性研究
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水生生态学
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蓝藻生物学
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微生物群落生态
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
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IF 7.2
Yuhui Xue,Changyan Guo,Jingjing Wang,Xueli Yang,Meng Wang,Jide Wang
The overuse of antibiotics poses a serious threat to the ecosystem. In particular, antibiotic residues in the aquatic environment are particularly serious. Therefore, preparation of efficient adsorbents for removing trace antibiotic residues from the environment becomes essential. In this work, a series of magnetic porous carbon material (Fe-MPC-gly) derived from MIL-101, which was regulated by glycine, were prepared by high temperature calcination for the adsorption of trace sulfonamide antibiotics (SAs) in water. The morphology of MIL-101 was found to gradually change from a regular ortho-octahedron to an acicular spindle with the increase as the glycine content increased. The results of adsorption experiments showed that Fe-MPC-gly-2 had the best adsorption effect on SAs. In particular, the adsorption of Fe-MPC-gly-2 on SAs could reach the adsorption equilibrium in 5 min, and the maximum adsorption amounts were 50.0 mg·g −1 and 38.2 mg·g −1 for sulfameter and sulfachloropyridazine, respectively. The adsorption kinetic model was best fitted to the quasi-first-order kinetic model. The possible adsorption mechanisms were pore filling, hydrogen bonding and π-π interactions. Meanwhile, the synthetic strategy of this study provides an idea for the preparation of efficient adsorbents for antibiotics.
研究领域:
环境科学
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材料科学
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化学工程
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纳米技术
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
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IF 6.1
Weishou Shen,Yang Wu,Futao Li,Shirui Zhang,Hongmei Jin,Bei Gao
Microplastics, as emerging contaminants, can absorb antibiotics, and their coexistence in soil ecosystems poses serious threats to soil health. While previous studies have primarily focused on the individual effects of microplastics or antibiotics, the interactions between these pollutants in soil environments remain poorly understood. In this study, we investigated the combined effects of sulfonamide antibiotics and microplastics—both non-degradable low-density polyethylene and degradable polylactic acid—on soil microbiota and physicochemical properties. Our findings revealed significant changes in soil properties under co-exposure conditions. Dissolved organic carbon emerged as the most influential factor affecting bacterial and fungal diversity. Co-exposure altered the composition of bacterial and fungal communities at both the phylum and genus levels, with soil bacteria showing stronger responses than fungi. Importantly, co-exposure exacerbated the ecological risks associated with individual contaminants. We also observed differences in how non-degradable and degradable microplastics impacted the stability and complexity of microbial community networks. Notably, co-exposure to degradable microplastics and sulfonamides led to a significant increase in the expression of antibiotic resistance genes ( sul1 and int1 ). These findings enhance our understanding of the combined effects of microplastics and antibiotics on soil ecosystems and underscore the need for further research into their ecological risks.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Desalination and Water Treatment
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2024
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IF 1
Wen-bin Li,Jiang-ming Ou-yang,Ke Xie,Hong-yan Deng,Yin-fei Wang,Touqeer Abbas,Bixia Wang
This study investigated the adsorption performance of antibiotics using modified semi-carbonized fibers (SCF) prepared from polyacrylonitrile and loaded with metal ions. Nitrated semi-carbonized fibers (N-SCF) and magnetized–nitrated semi-carbonized fibers (MN-SCF) were prepared through nitrification and magnetization methods, and different metal ion-loaded MN-SCFs were obtained by loading Ca and Mg ions onto MN-SCF. The effects of pH, temperature, and ionic strength on antibiotic adsorption were analyzed. The adsorption kinetics and thermodynamics of tetracycline (TC), chlortetracycline (CTC), and oxytetracycline (OTC) were studied using batch experiments. Results showed that (1) the adsorption of antibiotics on the tested materials initially increased and then decreased with increasing pH and ionic strength, reaching maximum values at pH 4 and 0.1 mol/L, respectively. (2) Higher temperatures facilitated the adsorption of antibiotics on the materials, which followed a chemisorption mechanism. (3) The Langmuir model provided a better fit for the adsorption isotherms of TC, CTC, and OTC compared with the Freundlich model, suggesting monolayer adsorption. (4) The maximum adsorption capacity ( q m ) of the antibiotics ranged from 0.80 to 3.97 mg/g, with of CTC > OTC > TC. Metal ion-loaded MN-SCFs exhibited higher adsorption amounts compared with MN-SCF alone.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
CHEMOSPHERE
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2020
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IF 5.778
Ye Yang,Kun Wan,Zhipeng Yang,Dailin Li,Guoxin Li,Songlin Zhang,Lei Wang,Xin Yu
Emerging contaminants such as antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) are becoming a global environmental problem. In this study, the glow discharge plasma (GDP) was applied for degrading antibiotic resistant Escherichia coli ( E. coli ) with resistance genes (tetA, tetR, aphA) and transposase gene (tnpA) in 0.9% sterile saline. The results showed that GDP was able to inactivate the antibiotic resistant E. coli and remove the ARGs and reduce the risk of gene transfer. The levels of E. coli determined by 16S rRNA decreased by approximately 4.7 logs with 15 min of discharge treatment. Propidium monoazide - quantitative polymerase chain reaction (PMA-qPCR) tests demonstrated that the cellular structure of 4.8 more logs E. coli was destroyed in 15 min. The reduction of tetA, tetR, aphA, tnpA genes was increased to 5.8, 5.4, 5.3 and 5.5 logs with 30 min discharge treatment, respectively. The removal of ARGs from high salinity wastewater was also investigated. The total abundance of ARGs was reduced by 3.9 logs in 30 min. Scavenging tests indicated that hydroxyl radicals (·OH) was the most probable agents for bacteria inactivation and ARGs degradation. In addition, the active chlorine (Cl· and Cl 2 ) which formed during the discharge may also contribute to the inactivation and degradation.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
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2019
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IF 5.714
Suyu Ren,Yi Wang,Ying Cui,Yan Wang,Xiaochun Wang,Jingwen Chen,Feng Tan
Tetracyclines (TCs) are frequently detected in agricultural soils worldwide, causing a potential threat to crops and human health. In this study, diffusive gradients in thin films technique (DGT) was used to measure the distribution and exchange rates of three TCs (tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC)) between the solid phase and solution in five farmland soils. The relationship between the accumulated masses with time suggested that TCs consumption in soil solution by DGT would induce the supply from the soil solid phase. The distribution coefficient for the labile antibiotics ( K dl ), response time ( T c ) and desorption/adsorption rates ( k b and k f ) between dissolved and sorbed TCs were derived from the dynamic model of DIFS (DGT induced fluxes in soils). The K dl showed similar sizes of labile solid phase pools for TC and OTC while larger pool sizes were observed for CTC in the soils. Although the concentrations of CTC were lowest in soil solution, the potential hazard caused by continuous release from soil particles could not be ignored. The long response time (>30 min in most cases) suggested that the resupply of TCs from soil solids was limited by their desorption rates (1.26-121 × 10 −6 s −1 ). The soils in finer texture, with higher clay and silt contents (<50 μm) showed a greater potential for TCs release.
研究领域:
环境科学
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土壤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-15
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IF 12.6
Zhang Tao,Liu Guiling,Sun Siwei,Meng Zongwu,Qiu Yuzhe,Ding Ping
The antibiotic residues pose significant risks for bacterial resistance. To address the practical requirements for rapid, accurate, and on-site detection of antibiotic residues and monitoring the abundance of associated resistance genes, we report a smartphone-integrated multi-mode platform. The platform is aimed to simultaneous, accurate, and visual quantitative detection of ampicillin (AMP) and β-lactam antibiotic resistance genes (blaTEM). Specifically, we developed a magnetically controlled fluorescence, colorimetric, and photothermal biosensor based on a magnetic separation unit (aminated modified complementary DNA chain (NH2-cDNA) loading on the surface of Ferrosoferric Oxide@polydopamine (Fe3O4@PDA, FP), FP@cDNA) and a signal unit (the aptamer nucleic acid chain modified by phosphate group linked to Prussian blue@UiO-66@manganese dioxide (PB@UiO-66@MnO2, PUM) through Zr-O-P bond, PUM@Apt), for the integrated detection of AMP and blaTEM. By utilizing complementary base pairing between FP@cDNA and PUM@Apt, along with precise aptamer recognition the AMP, we achieved the fluorescence quantitative detection of AMP by measuring the signal unit in the supernatant. Subsequently, the difference of signal units in colorimetric process leads to a varying conversion rate of oxidized 3,3’,5,5’-Tetramethylbenzidine (oxTMB), enabling the output of colorimetric and photothermal signals. The competitive binding of aptamers permitting the determination of AMP in the range of 0–160 pM with a low detection limit (0.34 pM). Additionally, in the presence of blaTEM, the activated CRISPR/Cas12a indiscriminately cleaves the single-stranded portion of the FP@DNA@PUM complex obtained by magnetic separation. A PUM-based three-signal detection scheme was established for the sensitive determination of blaTEM with the limit of detection (LOD) of 1.03 pM. The integration of smartphone-assisted analysis broadens the potential of the platform for visual detection. Notably, the innovative platform, with its excellent stability, exhibits great potential as a simple yet robust approach for the simultaneously visually monitoring antibiotics and drug resistance genes, and holds promise in the field of kit development.
研究领域:
生物化学
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分子生物学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
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IF 5.589
Yujia Xiang,Xiao Yang,Zhangyi Xu,Wenyong Hu,Yaoyu Zhou,Zhonghao Wan,Yuhui Yang,Yuyi Wei,Jian Yang,Daniel C.W. Tsang
An effective adsorbent towards fluoroquinolone antibiotics was synthesized via a facile two-step approach, the co-precipitation of Fe, Mn with vinasse wastes and then pyrolysis under controlled conditions which denoted as FMB. Its adsorption behavior was examined based on a batch adsorption experiment of fluoroquinolone antibiotics pefloxacin (PEF) and ciprofloxacin (CIP). Experimental factors, such as pH, adsorbent dose, ionic strength, contact time and temperature have done a great deal to influence the adsorption of PEF and CIP. The FMB demonstrated excellent performance in reusability tests towards to both PEF and CIP, which showed that the recycling efficiency of PEF and CIP could remain ~55% and ~80% after five recycle cycles, respectively. The dominated adsorption mechanisms included pore filling effect, π-π stacking interaction, π-π EDA, hydrogen bonding and hydrophobicity . Overall, this work presented FMB was recognized as an effective, environmental-friendly and magnetically separable adsorbent for alleviating fluoroquinolone antibiotics contamination from water.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-11
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IF 14.919
Wen Wan-Hong,Zhang Yue,Zhang Ying-Ying,Yu Qian,Jiang Chu-Chu,Tang Man-Cheng,Pu Jin-Yue,Wu Lian,Zhao Yi-Lei,Shi Ting,Zhou Jiahai,Tang Gong-Li
Antibiotic resistance is becoming one of the major crises, among which hydrolysis reaction is widely employed by bacteria to destroy the reactive pharmacophore. Correspondingly, antibiotic producer has canonically co-evolved this approach with the biosynthetic capability for self-resistance. Here we discover a self-defense strategy featuring with reductive inactivation of hemiaminal pharmacophore by short-chain dehydrogenases/reductases (SDRs) NapW and homW, which are integrated with the naphthyridinomycin biosynthetic pathway. We determine the crystal structure of NapW·NADPH complex and propose a catalytic mechanism by molecular dynamics simulation analysis. Additionally, a similar detoxification strategy is identified in the biosynthesis of saframycin A, another member of tetrahydroisoquinoline (THIQ) antibiotics. Remarkably, similar SDRs are widely spread in bacteria and able to inactive other THIQ members including the clinical anticancer drug, ET-743. These findings not only fill in the missing intracellular events of temporal-spatial shielding mode for cryptic self-resistance during THIQs biosynthesis, but also exhibit a sophisticated damage-control in secondary metabolism and general immunity toward this family of antibiotics.
研究领域:
微生物学
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药理学
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结构生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Weie Wang,Qinqin Sang,Ming Yang,Juan Du,Libin Yang,Xin Jiang,Xiaoxia Han,Bing Zhao
Herein, a surface-enhanced Raman scattering (SERS) strategy based on semiconducting substrate was exploited for detection of several antibiotic residues both in ultrapure water system and in actual water system. The as-prepared Ag-TiO 2 (Ag synchronously deposited and doped TiO 2 ) nanoparticle SERS-active substrate can achieve high sensitive SERS detection for difloxacin hydrochloride, ciprofloxacin, enrofloxacin, danofloxacin and enoxacin (five widely used quinolone antibiotics) in actual water samples, and the detection limits are as low as 4.36 × 10 −12 , 7.08 × 10 −11 , 3.94 × 10 −11 , 3.16 × 10 −11 and 3.15 × 10 −10 mol/L, respectively. These detection limits are far below the maximum of residue limit (3.01 × 10 −7 mol/L) stipulated by the European Union. And, the desirable quantitative relationships can be obtained in a wide concentration range. The recoveries of five antibiotic residues from spiked actual water samples are found to be more than 80.8% with the relative standard deviations between 2.1% and 4.7%. Even, the proposed SERS method can accurately distinguish every antibiotic species from a mixed antibiotic residue sample with multiple antibiotics. And, Ag-TiO 2 nanoparticles can also serve as an efficient photocatalyst for photocatalytic degradation of these antibiotic residues, which provides a multi-functional platform for synchronous determination and degradation of antibiotic residues in real environment.
研究领域:
环境科学
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分析化学
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药学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2022
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IF 13.4
Yongjie Liu,Yiwei Cai,Guiying Li,Wanjun Wang,Po Keung Wong,Taicheng An
The stress response of antibiotic-resistant bacteria (ARB) and the spread of antibiotic resistance genes (ARGs) pose a serious threat to the aquatic environment and human beings. This study mainly explored the effect of the heterogeneous photocatalytic oxidation (UVA-TiO 2 system) on the stress response mechanism of ARB with different antibiotic resistance action targets, including the cell wall, proteins, DNA, RNA, folate and the cell membrane. Results indicate that the stress response mechanism of tetracycline- and sulfamethoxazole-resistant E. coli DH5α, which targets the synthesis of protein and folate, could rapidly induce global regulators by the overexpression of relative antibiotic resistance action target genes. Different stress response systems were mediated via cross-protection mechanism, causing stronger tolerance to an adverse environment than other ARB. Moreover, the photocatalytic inactivation mechanism of bacterial cells and a graded response of cellular stress mechanism caused differences in the intensity of the stress mechanism of antibiotic resistance action targets. E. coli DH5α resistant to cefotaxime and polymyxin, targeting synthesis of the cell wall and cell membrane, respectively, could confer greater advantages to bacterial survival and higher conjugative transfer frequency than E. coli DH5α resistant to nalidixic acid and rifampicin, which target the synthesis of DNA and RNA, respectively. This new perspective provides detailed information on the practical application of photocatalytic oxidation for inactivating ARB and hampering the spreading of ARGs in the aquatic environment.
研究领域:
环境科学
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）