FISH & SHELLFISH IMMUNOLOGY
·
2025
·
IF 3.9
Yang Liu,Kai Liao,Yuxiang Zhu,Yang Zhang,Hailong Huang,Hui Ge,Jilin Xu
Taurine serves an important role in all aquatic animals. The purpose of this experiment was to investigate the effects of exogenous taurine on growth, endogenous taurine metabolism, and Vibrio crassostreae resistance in juvenile clam Ruditapes philippinarum . Microencapsulated taurine concentrations of 0, 0.75, 1.5, 3 and 6 mg/g clam wet weight were designated as the T0, T0.75, T1.5, T3 and T6 groups. After 2 weeks of feeding, the results demonstrated that exogenous taurine did not promote the growth of clams ( P > 0.05). The taurine content of clams was increased significantly with the increase of exogenous taurine content ( P < 0.05). The gene expression of taurine transporter ( TauT ) was significantly elevated in the T3 and T6 groups compared to the T0 group ( P < 0.05). With the addition of exogenous taurine, mRNA expressions of cysteine sulfinic acid decarboxylase ( CSAD ) , 2-aminoethylsulfide dioxygenase ( ADO ) , cysteine dioxygenase ( CDO ) , cystathionine β-synthase ( CBS ) , cystathionine γ-lyase ( CSE ) and serine dehydratase ( SDH ) were suppressed ( P < 0.05). The survival rate of clams challenged with Vibrio crassostreae was significantly higher in the T0.75 group compared to the T0 group on the fifth day ( P < 0.05). Additionally, the activities of antioxidant enzymes and lysozyme were significantly elevated, immune-related and anti-inflammatory factor genes were upregulated, while pro-inflammatory factor genes were downregulated in the T0.75 group compared to the T0 group ( P < 0.05). Dietary taurine supplementation regulates endogenous taurine metabolism and exhibits potential anti- Vibrio effect in clam R. philippinarum .
研究领域:
水产养殖学
·
分子生物学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2024
·
IF 6
Kai Wang,Wenzi Ren,Liang Hong,Qingao Wang,Rajendra Ghimire,Matti Haapanen,Minna Kivimäenpää,Pengfei Wu,Xiangqing Ma,Fred O. Asiegbu
Norway spruce is an important coniferous species in boreal forests. Root and stem rot diseases caused by the necrotrophic pathogen Heterobasidion parviporum threaten the wood production of Norway spruce which necessitates the search for durable control and management strategies. Breeding for resistant traits is considered a viable long-term strategy. However, identification of potential resistant traits and markers remains a major challenge. In this study, short-term disease resistance screening was conducted using 218 Norway spruce clones from 17 families. Disease resistance was evaluated based on the size of necrosis lesion length following infection with the pathogen. A subset of needles/branches from clones with small (partial resistant) or large (susceptible) lesions were used for terpene analysis and transcriptomic profiling. The results revealed that the content of monoterpene linalool and 1,8-cineole and their respective encoded genes were significantly more abundant and highly expressed in the partial resistant group. Furthermore, linalool and 1,8-cineole were demonstrated to have inhibitory effect on the growth of the pathogen H. parviporum , with morphological distortion of the hyphae. RNAseq analysis revealed that transcript of pathogen genes involved in the regulation of carbohydrate metabolism and stress responses were significantly decreased in presence of the terpenes. The results suggest the relevance of monoterpenes together with jasmonic acid precursor and some genes involved in phenylpropanoid biosynthesis, as constitutive tolerance factors for Norway spruce tolerance against necrotrophic pathogen. The high level of necrosis related cell death gene expression might be factors critical for host susceptibility and disease development.
研究领域:
植物学
·
分子生物学
·
林学
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产品关联
DOI
PubMed
高影响因子
Phytopathology Research
·
2024
·Vol. 6
·期 1
·1-15
·
IF 3.2
Li Xiufang,Liu Zongling,Wu Haoming,Yu Zhuoxin,Meng Jiaorong,Zhao Haiyun,Deng Xingli,Su Yizu,Chen Baoshan,Li Ru
Sugarcane ( Saccharum spp.) is a major sucrose and bioenergy crop in the world. The fungal pathogen Sporisorium scitamineum causes sugarcane smut, a devastating disease that destroys stalks and reduces sugar content in sugarcane. This disease can be controlled most effectively by applying smut-resistant sugarcane varieties. Previous studies have shown that Dirigent (DIR ) genes are involved in the synthesis of the lignin precursor pinoresinol, which plays a crucial role in plant resistance to biotic stresses. However, the immune response of the DIR homologs in sugarcane ( ScDIR ) has not been reported yet. In this study, we found that the lignin content of smut-resistant sugarcane varieties (ZZ1, ZZ6, and ZZ9) was significantly higher than that of smut-susceptible varieties (GT42, ROC22, and FN41), and the lignin content of sugarcane increased after smut infection. The smut-resistant and smut-susceptible clones derived from the same genetic population (ROC25 × YZ89-7) showed similar patterns. Quantitative real-time PCR assays revealed that among the 64 DIR genes in sugarcane, ScDIR5 , ScDIR7 , ScDIR11 , and ScDIR40 showed elevated expression after S. scitamineum infection. In vitro coupling reactions showed that the four corresponding ScDIR proteins could mediate the coupling of coniferyl alcohol and its conversion into the lignin precursor pinoresinol. Overexpression of the four ScDIR genes in Nicotiana benthamiana enhanced disease resistance to the fungal pathogens Sclerotium rolfsii , Rhizoctonia solani , and Botrytis cinerea . Moreover, transgenic sugarcane overexpressing these ScDIR genes showed enhanced resistance to smut disease. Taken together, our findings provide evidence that sugarcane ScDIR genes can improve the resistance of plants to fungal pathogens and highlight their potentials in sugarcane breeding for disease resistance.
研究领域:
植物病理学
·
分子生物学
·
遗传学
·
农业科学
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Kun Gao,Jiang Zhan,Yukun Qin,Song Liu,Ronge Xing,HuaHua Yu,Xiaolin Chen,Pengcheng Li
Plant fungal diseases can lead to yield reduction and quality degradation in crops, which usually cause serious economic losses. Additionally, chemical fungicides used in the prevention and control of plant diseases are increasingly restricted due to resistance development and high toxicity. Therefore, biogenic fungicides such as chitosan with low toxicity and good biocompatibility are receiving increasing attention. This study found that the acid swelling chitosan pretreatment method can accelerate the rate of the specific oxidation of chitosan catalyzed by the TEMPO-NaBr-NaOCl system. This study proved that OCTS induces plant disease resistance, and the control efficiencies achieved in protection and treatment experiments against Botrytis cinerea were 80.6 % and 83.4 %, respectively, at 400 μg/mL OCTS. In addition, OCTS can promote plant growth and enhance plant defense enzyme activities. This research has realized a forward-looking exploration of the application of OCTS in the agricultural field.
研究领域:
植物病理学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PLANT GROWTH REGULATION
·
2022
·Vol. 42
·期 3
·1558-1566
·
IF 4.64
Zhang Lan,Zhang Zheng,Ahammed Golam Jalal,Wang Xiaorong,Fang Huihan,Yan Peng,Zhang Li-Ping,Ge Shibei,Fu Jianyu,Han Wen-Yan,Li Xin
Brassinosteroids (BRs) are steroid-type phytohormones that not only control growth and development but also responses to biotic and abiotic stressors in plants. However, little is known about the function of BRs in the protection of tea against the fungal pathogen Colletotrichum fructicola causing anthracnose disease. Here, we revealed that 24-epibrassinolide (EBR) could improve tea resistance against C. fructicola , which was linked to the EBR-induced accumulation of lignin in tea leaves. Time-course of the relative expression of ITS/Cs18SrDNA showed that its expression increased after the inoculation with C. fructicola ; however, EBR pretreatment significantly decreased the ITS/Cs18SrDNA expression at 3, 24 and 96 h postinoculation. Following an initial increase in 3 h postinoculation, lignin content significantly decreased from 6 to 24 h postinoculation with C. fructicola . In contrast to inoculation with only C. fructicola , exogenous EBR pretreatment significantly increased lignin contents throughout the postinoculation period. According to the qRT-PCR assay, EBR and C. fructicola differentially regulated the expression of 14 genes involved in the phenylpropanoid and lignin-specific pathways. Moreover, the activity of peroxidase (POD), a central enzyme in the final phase of lignin biosynthesis increased significantly at 3 h postinoculation with EBR treatment. Gene expression analysis revealed that EBR induced CsMYB4-like expression possibly to activate the transcription of CsPOD5-like gene responsible for lignin biosynthesis in response to C. fructicola . These findings suggest that EBR plays a positive role in disease resistance by inducing lignin synthesis, which may have potential application for the prevention of C. fructicola in tea plants.
研究领域:
植物病理学
·
植物生理学
✓ 已验证
DOI
高影响因子
Agronomy-Basel
·
2020
·Vol. 10
·期 2
·265
·
IF 2.603
Cheng Zhang,Youhua Long,Jiaohong Li,Ming Li,Deke Xing,Huaming An,Xiaomao Wu,Yanyou Wu
Soft rot caused byBotryosphaeriadothideaandPhomopsissp. is a critical disease in kiwifruit. In order to efficiently control soft rot, a 28.6% chitosan composite film (CCF) containing chitosan, dextrin, ferulic acid, calcium, and auxiliaries was successfully developed. The results showed that CCF had a strong inhibitory effect on mycelia growth ofB. dothideaandPhomopsissp., with mycelial EC50values of 68.11 and 50.34 mg L−1, respectively. The concentration of 0.71–1.42 g L−1CCF had noticeably preventive and curative effects against soft rot. The spray of CCF before pathogen infection effectively reduced the incidence of soft rot, remarkably increased the content of resistance compounds, and activated the activity of defense enzymes. Moreover, it notably enhanced the yield and quality and prolonged the shelf life of kiwifruit. Therefore, the excellent control effects of CCF against soft rot might be associated with its film-forming property and antifungal activity, which prevent infection and induce plant defense mechanisms. The concentration of 0.71–1.42 g L−1CCF was optimal for the field application before the onset of disease symptoms in plants withB. dothideaandPhomopsissp.Keywords:chitosan composite film;kiwifruit;pathogen infection;soft rot;disease resistance;storage quality
研究领域:
植物病理学
·
食品科学
✓ 已验证
DOI
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 7
·1699
·
IF 4.2
Aiguo Huang,Yanqin Wei,Jialong Huang,Songlin Luo,Tingyu Wei,Jing Guo,Fali Zhang,Yinghui Wang
Streptococcus agalactiae(SA) is a severe prevalent pathogen, resulting in high morbidity and mortality in the global tilapia industry. With increasing bacterial resistance to antibiotics, alternative strategies are urgently needed. This study aims to investigate the antibacterial activity and the underlying mechanisms of the natural product xanthohumol (XN) against SA infection in tilapia (Oreochromis niloticus). The results showed that XN could significantly reduce the bacterial loads of SA in different tissues (liver, spleen and brain) after treatment with different tested concentrations of XN (12.5, 25.0 and 50.0 mg/kg). Moreover, XN could improve the survival rate of SA-infected tilapia. 16S rRNA gene sequencing demonstrated that the alpha-diversity index (Chao1 and Shannon_e) was significantly increased in the XN-treated group (MX group) compared to the SA-infected group (CG group) (p< 0.05), and the Simpson diversity index significantly decreased. The Bray–Curtis similarity analysis of non-metric multidimensional scaling (NMDS) and principal coordinate analysis (PCA) showed that there were significant differences in microbial composition among groups. At the phylum level, the relative abundance of the phyla Actinobacteria, Proteobacteria and Bacteroidetes decreased in the MX group compared to the CG group, while the relative abundance of the phyla Fusobacteria, Firmicutes and Verrucomicrobia increased. Differences were also observed at the genus level; the relative abundance ofMycobacteriumdecreased in the MX group, but the abundance ofCetobacteriumandClostridium_sensu_stricto_1increased. Metabolomics analysis revealed that XN changed the metabolic profile of the liver and significantly enriched aspartate metabolism, glycine and serine metabolism, phosphatidylcholine biosynthesis, arginine and proline metabolism, glutamate metabolism, urea cycle, purine metabolism, methionine metabolism, betaine metabolism, and carnitine synthesis. Correlation analysis indicated an association between the intestinal microbiota and metabolites. In conclusion, XN may be a potential drug for the prevention and treatment of SA infection in tilapia, and its mechanism of action may be related to the regulation of the intestinal microbiota and liver metabolism.
研究领域:
微生物学
·
鱼类免疫学
·
代谢组学
·
水产药理学
·
肠道生态学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 48
·28576-28584
·
IF 3.119
Chao Feng,Xingling Tian,Xiaoqiang Wang,Mengmeng Cui,Chuantao Xu,Weimin Wang,Wei Wang
We synthesised a silicon dioxide nanosphere with a novel nanostructure by loading salicylic acid (SA) as a plant disease resistance inductor to prolong plant life. The SA nanosphere was evaluated by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, N2 adsorption method, enzyme activity test and pot experiments. The results demonstrated that the SA nanosphere induced the activities of polyphenol oxidase, phenylalanine ammonia-lyase, peroxidase, and chitinase to enhance plant immunity to inhibit Phytophthora nicotianae. Its SA loading capacity reached approximately 80%. The SA nanospheres exhibited a sustained release and maintained its resistance effect at 84.79% after 15 days. Thus, the SA nanospheres could gradually release SA to enhance inhibitive enzyme activity in diseased plants. Furthermore, finite element method was used to establish different nanosphere models and analyse the SA releasing process. SA concentration sharply increased near the nanospheres, and SA was slowly released to the solution. This SA nanosphere will have a great potential in future environmental-friendly practical application.
研究领域:
植物学
·
纳米技术
·
植物病理学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PLANT PHYSIOLOGY
·
2023
·
IF 4.3
Guofu Luo,Yirui Shen,Kun Wu,Huining Yang,Chuntao Wu,Xiangbing Chang,Wei Tian
Application of elicitors can greatly enhance plant immune resistance against pathogens. However, it is still obscure whether elicitor activity is influenced by diverse sources. This study investigated the effect of foliar spraying of 19 batches of Chrysanthemum indicum polysaccharides (CIPs) on the disease resistance of Atractylodes macrocephala Koidz. ( A. macrocephala ) and explored the main reasons for the differences of inducing activity of CIP elicitors. PCA, OPLS-DA, grey relational analysis and entropy weight method had good predictability for the activity evaluation of CIP elicitors and other plant-derived elicitors. The results showed that 19 batches of CIPs had definite regional differences in inducing activity and monosaccharide content. CIP elicitors with high inducing activity could significantly increase the accumulation of Atractylenolide Ⅱ and Atractylenolide Ⅲ, the mRNA relative transcription level of CAT , POD , PAL genes, the amount of pH change in the medium and effectively reduce the disease index of A. macrocephala . Furthermore, CIP with high inducing activity exhibited the high contents of Rha, Ara and GalA, which might be the main contributor to their high activity. The evaluation procedure developed in this work can be applied for screening CIP elicitors with high inducing activity, and it lays a foundation for identifying more functional elicitors related to plant immune resistance.
研究领域:
植物科学
·
药用植物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Reports
·
2023
·Vol. 42
·期 12
·113480-113480
·
IF 8.8
Liang Y,Meng F,Zhao X,He X,Liu J
The endoplasmic reticulum (ER) is the largest intracellular endomembrane system; it shows dynamic changes upon environmental stress. To maintain ER morphology and homeostasis under stress, the excessive ER membrane and the associated unwanted proteins can be removed via ER-phagy. Although a few ER-phagy receptors have been reported in mammals and yeast, their functional counterparts in plants remain largely unexplored. Here, we report that the HVA22 family protein OsHLP1 is an uncharacterized ER-phagy receptor in rice (Oryza sativa L.). OsHLP1 interacts with OsATG8b and recruits ER subdomains and the cargo protein OsNTL6, a negative immune regulator, to autophagosomes upon infection with the fungus Magnaporthe oryzae, which substantially activates disease resistance in rice. AtHVA22J, an Arabidopsis thaliana OsHLP1 ortholog, induced similar ER-phagy in plants. Altogether, we unraveled a conservative protein family that may act as ER-phagy receptors in higher plants, and in particular, we highlighted their roles in rice immune responses.
研究领域:
植物生物学
·
分子生物学
·
植物病理学
✓ 已验证
DOI
PubMed
高影响因子
PLANT JOURNAL
·
2025
·Vol. 123
·期 4
·e70432
·
IF 5.7
Xiaoqing He,Shiyu Ying,Yi Xu,Zhuo Gao,Yi Wu,Mingchun Liu,Mengbo Wu
SUMMARY Fruits constitute a vital component of a nutritious diet but are highly perishable, contributing substantially to food waste. Consequently, identifying safe and edible biological agents to enhance product quality and extend shelf life is of critical importance. In this study, we demonstrate that exogenous pre-harvest foliar application with nanoselenium (nano-Se) in tomato enhances fruit quality, prolongs fruit shelf life, and enhances the resistance of tomato fruit to Botrytis cinerea infection. Transcriptomic analysis revealed coordinated upregulation of genes associated with quality maintenance and modulation of phytohormone-related pathways. Notably, nano-Se treatment induced expression patterns of ripening-related genes that resembled those triggered by ethylene (ET) but were antagonistic to the effects of 1-MCP. We further demonstrated that although SlMYC2 knockout increased susceptibility to B. cinerea , nano-Se application restored resistance in a manner independent of the SlMYC2-associated jasmonic acid signaling pathway, implicating ET as the primary regulatory mechanism. Collectively, these findings support nano-Se as a promising biostimulant for reducing post-harvest losses while preserving the nutritional and sensory quality of tomato fruits.
研究领域:
植物科学
·
园艺学
·
纳米技术
·
采后生物学
·
分子生物学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Laifeng Lu,Lifeng Ji,Qingqing Ma,Mingguan Yang,Shuhua Li,Qiong Tang,Liping Qiao,Fengjuan Li,Qingbin Guo,Changlu Wang
The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously high expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack. Pectin induced the antagonistic yeast to secrete pectinolytic enzymes to increase fruit resistance against gray mold. The activities of pathogenic pectinase of B. cinerea were correspondingly depressed in the pectin-inducible yeast enzyme elicited ripe fruit. These data suggest that pectinase is a molecular target for regulation of disease resistance during fruit ripening.
研究领域:
植物病理学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）