INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mianzhang Zhang,Hongliang Li,Fu Liu,Shiyi Ou,Pengzhan Liu,Jie Zheng
Carbonyl stress contributes to pathological disorders leading to the progression of a variety of chronic diseases. Exploration of food ingredients with carbonyl scavenging capacities became one of the most potential strategies for the prevention of these diseases. Polysaccharide-based hydrocolloids have wide application approaches in the food industry. However, the carbonyl scavenging capacity of some natural polysaccharide-based hydrocolloids is unsatisfactory due to structural limitations. In this research, gallic acid (GA) was grafted onto different hydrocolloids (xanthan gum, carrageenan, carboxymethylcellulose, carboxymethyl chitosan (CMCS), and sodium alginate) by a free radical-mediated reaction to improve their antioxidant, carbonyl scavenging, and anti-glycation activities. The antioxidant activity of hydrocolloids was significantly improved after GA grafting. Among them, CMCS-GA (graft ratio 15.45 mg GA/g) exhibited the same ABTS scavenging ability as the equivalent amount of free GA and Vc. The carbonyl scavenging capacity, and the inhibitory effects on the fluorescent advanced glycation end products (AGEs) and protein oxidation products of some hydrocolloids also increased significantly GA grafting. The scavenging capacity of CMCS-GA on MGO, GO, ACR, and MDA in the model reaction increased by 220 %, 100 %, 6 %, and 58 %, respectively. Overall, CMCS-GA showed the best performance in radical scavenging, carbonyl scavenging, and AGEs inhibition compared to all the other hydrocolloids investigated. The findings showed that grafting GA onto hydrocolloids is a viable strategy to enhance the carbonyl scavenging capacity and anti-glycation activity of hydrocolloids.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Halah Aalim,Tarun Belwal,Lei Jiang,Hao Huang,Xianghe Meng,Zisheng Luo
The purpose of this investigation was to explore aqueous glycerol as food grade solvent for rice bran polyphenolic antioxidants extraction and investigate the antidiabetic and antiglycation potential of the extract and its major polyphenols. Response surface methodology (Box-Behnken design) was implemented to optimize experimental conditions for rice bran polyphenols and antioxidants extraction. The optimum conditions provided a very satisfactory outcome in total polyphenols (549.6 ± 8.05 mg gallic acid equivalent per 100 g dry weight) and DPPH scavenging activity (57.83 ± 1.8%). The chromatographic separation of the extract showed higher concentrations of ferulic acid, ρ -coumaric acid and chlorogenic acid followed by caffeic acid, syringic acid and rutin. Moreover, the extract inhibited α-amylase, α-glucosidase, and lipase activity as 34.2 ± 3.2%, 29.9 ± 1.0%, and 12.4 ± 2.3%, respectively. Furthermore, fructose readily forms Advanced Glycation End Products (AGEs) fluorescence with basic amino acids in low concentration in simulated intestinal digestion conditions. The extract inhibited AGEs formation by 52.6% at 20 μL/mL. Our study indicated that the antidiabetic and antiglycation potential of rice bran extract was mainly driven by the extract polyphenols. Rice bran glycerol extract could be employed as a natural antidiabetic and antiglycation remedy, and as economically and eco-friendly source of potent bioactive compounds with satisfactory outcome.
研究领域:
食品科学
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植物化学
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药理学
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2022
·
IF 5.572
Yejun Deng,Xiang Wang,Yang Zhang,Caihong Zhang,Pujun Xie,Lixin Huang
The aim of this study was to investigate the antiglycation activity and mechanism of two identified peptides, Valine-Valine-Phenylalanine-Proline-Glycine-Cysteine-Proline-Glutamic acid (VVFPGCPE) and Serine-Valine-Aspartic acid-Aspartic acid-Proline-Arginine-Threonine-Lysine (SVDDPRTL), from Ginkgo biloba seeds protein hydrolysates . Both VVFPGCPE and SVDDPRTL were efficient in bovine serum albumin (BSA)-methylglyoxal (MGO) model to inhibit BSA glycation, while VVFPGCPE showed higher antiglycation activity than SVDDPRTL. In antioxidant assays, VVFPGCPE scavenged more hydroxyl and super anion radicals, and chelated more Fe 2+ . Moreover, VVFPGCPE was more efficient in alleviating glycoxidation since it retained higher content of tryptophan and reduced dityrosine and kynurenine generation. Compared with SVDDPRTL, VVFPGCPE showed better performance in inhibiting protein aggregation and amyloid-like fibrillation formation. Therefore, VVFPGCPE was selected for further mechanism study. The circular dichroism analysis suggested VVFPGCPE could preserve α -helix structure and stabilize protein structure. The MGO trapping assay indicated VVFPGCPE (5 mg/mL) could capture 66.25% MGO within 24 h, and the mass spectrometry revealed VVFPGCPE could trap MGO by forming VVFPGCPE-mono-MGO adducts. Besides, molecular simulations suggested VVFPGCPE could interact with key glycation residues, arginine and lysine residues, of BSA mainly through van der Waals and hydrogen bonds . This study might supply a theoretical basis for the development of VVFPGCPE as an effective antiglycation agent.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Food & Function
·
2016
·Vol. 7
·期 9
·3953-3963
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IF 2.686
Li Zeng,Guowen Zhang,Yijing Liao,Deming Gong
It is important to investigate the inhibition of α-glucosidase due to its correlation with type 2 diabetes. Morin was found to exert significant inhibition activity on α-glucosidase in a reversible mixed-type manner with an IC50 value of (4.48 ± 0.04) μM. Analyses of fluorescence and circular dichroism spectra indicated that the formation of the morin-α-glucosidase complex was driven mainly by hydrophobic forces and hydrogen bonding, and caused the conformational changes of α-glucosidase. The phase diagrams of fluorescence showed that the conformational change process was monophasic without intermediates. Molecular docking indicated that morin mainly interacted with amino acid residues located close to the active site of α-glucosidase, which may move to cover the active pocket to reduce the binding of the substrate and then inhibit the catalytic activity. Morin was also found to exhibit inhibition in the generation of advanced glycation end products which was related to the long term complications of diabetes.
研究领域:
药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2022
·Vol. 16
·期 1
·232-246
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IF 5.581
Deng Yejun,Liu Yong,Zhang Caihong,Xie Pujun,Huang Lixin
The accumulation of advanced glycation end-products (AGEs) from food matrix in human body can induce health crisis. This study firstly investigated the AGEs accumulation reducing potential of soluble dietary fiber (SDF) from Rhodomyrtus tomentosa fruits in antiglycation and trapping AGEs aspects. To obtain superior functionalities, the SDF was prepared by an enzymatic modified extraction method (ESDF). The result showed the extraction yield of SDF increased by 45% with the Viscozyme L modification. The structural characterizations indicated the enzymatic treatment greatly influenced the monosaccharide composition, thermal stability, crystallinity, and apparent viscosity of Rhodomyrtus tomentosa fruits SDF (RTSDF) as compared with SDF obtained from water extraction method (WSDF). The antioxidant assay indicated that RTSDF could scavenge O 2−∙ ,OH ∙ , and H 2 O 2 radicals and chelate Fe 2+ . Additionally, RTSDF, especially ESDF, reduced the more fluorescent AGEs generation in bovine serum albumin (BSA)-fructose model, and protected the BSA from oxidation and cross-linking. Besides, the RTSDF presented the ability to trap those already formed BSA-bound AGEs. ESDF exhibited higher AGEs binding capacity, while WSDF demonstrated stronger ability in restraining the AGEs diffusion. These results suggested the potential utility of RTSDF serving as an essential ingredient in functional foods to reduce AGEs accumulation in human body, controlling AGEs-associated diseases.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
·
IF 6.6
Xin Cheng,Dao Xiao,Xiaoyan Zheng,Yang Yang,Lili Zheng,Binling Ai,Shenwan Wang,Zhanwu Sheng
Banana blossoms, a natural and sustainable source, were successfully prepared to produce carbon dots (BB-CDs) with promising anti-glycation properties. Advanced glycation end products (AGEs), implicated in various degenerative diseases, were significantly reduced in food models fortified with the BB-CDs. Spherical in shape and measuring 2.06 nm, the BB-CDs possessed a rich surface chemistry, brimming with hydrophilic and reactive functional groups. The multi-stage kinetic analysis results revealed that BB-CDs inhibited the formation and accumulation of AGEs by reducing the conversion of reaction substrates to intermediates and intermediates into end products, while simultaneously enhancing the degradation of end products. In practical application, sponge cakes fortified with 2.5 g/100 g BB-CDs exhibited marked reductions in AGEs, including N ε -carboxymethyllysine and N ε -carboxyethyllysine, by over 40%, both in free and protein-bound forms. Concurrently, BB-CDs improved the moisture retention and texture of sponge cakes, softening their structure. These findings demonstrate that BB-CDs is a novel exogenous inhibitor of AGEs, which can be applied to mitigate the production of harmful substances during thermal processing of food.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 10
·1573
·
IF 4.7
Shufen Wu,Changyan Dong,Meihui Zhang,Yi Cheng,Xiaobo Cao,Benxu Yang,Chao Li,Xin Peng
Red yeast rice dietary supplements have been proven to ameliorate hyperglycemia, but the mechanism was unclear. In this work, ankaflavin (AK) and monascin (MS), as typical pigments derived from red yeast rice, were found to exert noteworthy inhibitory ability against α-glucosidase, with an IC50of 126.5 ± 2.5 and 302.6 ± 2.5 μM, respectively, compared with acarbose (IC50= 341.3 ± 13.6 μM). They also exhibited mixed-type inhibition of α-glucosidase in vitro and caused fluorescence quenching through the static-quenching process. Molecular-docking studies indicated that AK and MS bind to amino acid residues outside the catalytic center, which induces structural changes in the enzyme, thus influencing its catalytic activity. The anti-glycation ability ofMonascus-fermented products was evaluated, and they exhibited a high inhibition rate of 87.1% in fluorescent advanced glycation end-product formation at a concentration of 0.2 mg mL−1, while aminoguanidine showed a rate of 75.7% at the same concentration. These results will be significant in broadening the application scope ofMonascuspigments, especially AK and MS, in treating type 2 diabetes.
研究领域:
药理学
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营养学
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糖尿病研究
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2012
·
IF 3.655
Qian Wu,Hengye Chen,Zhejuan Lv,Shuyi Li,Bei Hu,Yafei Guan,Bijun Xie,Zhida Sun
It has been reported that oligomeric procyanidins of lotus seedpod (LSOPC) is effective in the alleviation of Alzheimer’s disease and diabetes through its antioxidant and insulin-potentiating activities. This study investigated the anti-glycative activity of LSOPC in a bovine serum albumin (BSA)-glucose model. The level of glycation and conformational alterations were assessed by specific fluorescence, Congo red binding assay and circular dichroism. The results show that LSOPC has a significant anti-glycative activity in vitro and it can also effectively protect the secondary structure of BSA during glycation. LSOPC or catechin (a major constituent unit of LSOPC), were used to react with methylglyoxal. The structures of their carbonyl adducts were tentatively identified using HPLC-MS 2 . Their capacity to scavenge methylglyoxal suggested carbonyl scavenging as a major mechanism of antiglycation. Therefore, LSOPC could be helpful to prevent AGEs-associated diseases, and with the potential to be used as functional food ingredients.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 23
·3941
·
IF 4.7
Chunxia Zhou,Shanshan He,Shang Gao,Zirui Huang,Wenduo Wang,Pengzhi Hong,Rui-Bo Jia
The aim of this study was to investigate the effect of ultrasonic treatment on the physicochemical properties and bioactivities of polysaccharides fromSargassumsamples (SPs) extracted with different solvents. The alkali-assisted extraction of polysaccharide (SPA), acid-assisted extraction of polysaccharides from (SPB), and hot water extraction of polysaccharides (SPCs) were perofrmed onSargassum. Ultrasonic treatment was performed with the SPA, SPB, and SPC in turn, and named USPA, USPB, and UPSC, respectively. The results showed that SPs mainly consisted of mannose, glucose, xylose, rhamnose, galactose, fucose, glucuronic acid, mannuronic acid and guluronic acid. The molecular weight of SPA (434.590 kDa) was the lowest under different solvent extractions, and the molecular weights of SPA, SPB, and SPC were reduced after sonication. SPA had a high carbohydrate content of (52.59 ± 5.16)%, and SPC possessed a high sulfate content of (3.90 ± 0.33)%. After ultrasonic treatment, the biological activities of SPs were significantly increased. Theα-glucosidase inhibition assay reflected that the IC50 values of the ultrasonic treatment SPs were significantly reduced, and USPA showed the best activity, with an IC50 of (0.058 ± 0.05) mg/mL. Antioxidant assays demonstrated that USPC exhibited greater DPPH- and ABTS-scavenging capacity. In the anti-glycosylation assay, SPs after sonication demonstrated excellent inhibition of glycosylation products and protein oxidation products, with USPA showing the highest inhibition rate. In conclusion, the biological activities of SPs were enhanced after ultrasonic treatment. This study provides a theoretical reference for their use in food and medicines.
研究领域:
食品科学
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海洋生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2023
·
IF 7.3
Zhou Huiji,Zhou Luxian,Li Bo,Yue Rongcai
Introduction: Dendrobium officinale Kimura et Migo (D. officinale) , widely called as “life-saving immortal grass” by Chinese folk, is a scarce and endangered species. The edible stems of D. officinale have been extensively studied for active chemical components and various bioactivities. However, few studies have reported the well-being beneficial effects of D. officinale flowers (DOF). Therefore, the present study aimed to investigate the in vitro biological potency of its aqueous extract and screen its active components.Methods: Antioxidant tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the ferric reducing ability of plasma (FRAP), and intracellular reactive oxygen species (ROS) level analyses in primary human epidermal keratinocytes, anti-cyclooxygenase2 (COX-2) assay, anti-glycation assay (both fluorescent AGEs formation in a BSA fructose/glucose system and glycation cell assay), and anti-aging assay (quantification of collagen types I and III, and SA-β-gal staining assay) were conducted to determine the potential biological effects of DOF extracts and its major compounds. Ultra-performance liquid chromatography-electrospray ionisation-quadrupole-time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS/MS) was performed to investigate the composition of DOF extracts. Online antioxidant post-column bioassay tests were applied to rapidly screen major antioxidants in DOF extracts.Results and discussion: The aqueous extract of D. officinale flowers was found to have potential antioxidant capacity, anti-cyclooxygenase2 (COX-2) effect, anti-glycation potency, and anti-aging effects. A total of 34 compounds were identified using UPLC-ESI-QTOF-MS/MS. Online ABTS radical analysis demonstrated that 1-O-caffeoyl-β-D-glucoside, vicenin-2, luteolin-6-C-β-D-xyloside-8-C-β--D-glucoside, quercetin-3-O-sophoroside, rutin, isoquercitrin, and quercetin 3-O-(6″-O-malonyl)-β-D-glucoside are the major potential antioxidants. In addition, all selected 16 compounds exerted significant ABTS radical scavenging ability and effective AGE suppressive activities. However, only certain compounds, such as rutin and isoquercitrin, displayed selective and significant antioxidant abilities, as shown by DPPH and FRAP, as well as potent COX-2 inhibitory capacity, whereas the remaining compounds displayed relatively weak or no effects. This indicates that specific components contributed to different functionalities. Our findings justified that DOF and its active compound targeted related enzymes and highlighted their potential application in anti-aging.
研究领域:
药理学
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生物化学
·
植物科学
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化妆品科学
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产品关联
DOI
PubMed
高影响因子
Food Production Processing and Nutrition
·
2020
·Vol. 2
·期 1
·1-7
·
IF 0
Hu Xiaoqian,Wang Mingfu,Shahidi Fereidoon
AbstractTo expand the application of tyrosol, a series of lipophilized tyrosol derivatives were synthesized via esterification of tyrosol with fatty acids of different chain lengths. The antiglycative activity of tyrosol esters so prepared was subsequently examined in the bovine serum albumin/glucose system. A quasi-parabolic shape was observed when the activity was plotted against alkyl chain length. Additionally, the anti-inflammatory effects of these derivatives were evaluated against methylglyoxal-induced inflammation in RAW264.7 cells. The same trend on anti-inflammatory activity was found as in the antiglycation study. The results showed that tyrosol esters with C12:0 and C14:0 were two most efficient ones among all the tested derivatives. Thus, some lipophilized tyrosol derivatives were stronger antiglycative and anti-inflammatory agents compared to the parent compound, tyrosol.Graphical abstract
研究领域:
药理学
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化学
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生物化学
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DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Guihong Yu,Xiangji Fu,Xuhua Mo,Lingling Tan,Song Yang
Bioactivity screening revealed that the EtOAc extract from the culture broth of Phellinus igniarius SY489 exhibited remarkable α -glucosidase inhibitory activity, with an IC 50 value of 1.92 μg/mL. Activity- and ultraviolet (UV) profile-guided isolation led to the discovery of four anti-diabetic styrylpyrones ( 1 – 4 ), including two novel compounds, phelignidins A ( 1 ) and B ( 2 ). Compounds 1 and 2 represent a rare structural type of styrylpyrone dimer, in which one of the pyrone moieties exists in an open-ring state. The absolute configurations of the new compounds 1 and 2 , as well as the previously unresolved compound 3 , were established. Compounds 1 – 4 were effective in α -glucosidase inhibition, anti-glycation, and antioxidant assays, surpassing or being comparable to the positive control drugs, with minimal cytotoxicity. Furthermore, studies on α -glucosidase inhibition mechanisms suggested that these compounds interact with α -glucosidase at a single binding site, causing secondary structure unfolding and exerting inhibitory activity via a mixed-type mechanism. These results provide an important basis for developing novel, low-toxicity, multi-target anti-diabetic drugs from edible and medicinal fungi.
研究领域:
药理学
·
药物化学
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真菌学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）