INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Shenwan Wang,Yang Yang,Dao Xiao,Xiaoyan Zheng,Binling Ai,Lili Zheng,Zhanwu Sheng
Musa spp. (banana) is consumed globally as a healthy fruit and improves the immune system. Banana blossoms are a by-product of banana harvesting rich in active substances such as polysaccharides and phenolic compounds; however, these blossoms are typically discarded as waste. In this report, a polysaccharide, MSBP11, was extracted, purified and identified from banana blossoms. MSBP11 is a neutral homogeneous polysaccharide with a molecular mass of ∼214.43 kDa and composed of arabinose and galactose at a ratio of 0.303:0.697. MSBP11 exhibited potent antioxidant and anti-glycation activities in a dose-dependent manner and can be used as a potential natural antioxidant and inhibitor of advanced glycosylation end products (AGEs). In addition, banana blossoms have been shown to decrease the levels of AGEs in chocolate brownies, which might possibly be developed as functional foods for diabetic patients. This study provides a scientific basis to further research the potential application of banana blossoms in functional foods.
研究领域:
食品科学
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植物化学
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营养学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2021
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IF 9.147
Gang Yu,Qiaozhi Zhang,Yanbo Wang,Qing Yang,Hejun Yu,Huan Li,Jian Chen,Linglin Fu
Marine red algae are rich sources of sulfated polysaccharides as bioactive ingredients and hydrocolloid molecules. In this study, sulfated polysaccharides (YGAP, EGAP, and NGAP) were extracted from Gelidium amansii s that were harvested from three different sea areas of China. Characterization of these polysaccharides shows that they contain 3.7–4.0% sulfate and are mainly composed of galactose , glucose, fucose, arabinose, and mannose . The FT-IR and 13 C NMR analysis indicate that the sulfated polysaccharides are agar-type galactans containing 3,6 anhydro-α- l -galactopyranose and β- d -galactopyranose residues. By using various in vitro models, all sulfated polysaccharides, particularly EGAP, exhibit the ability to scavenge O 2 •– and OH· radicals and reducing power. Additionally, in a glucose-glycated BSA system, the polysaccharides show an inhibitory effect on protein glycation and formation of specific advanced glycation end-product. Their anti-glycative property is attributed to their anti-oxidant activity and trapping capacity of methylglyoxal. Given that these polysaccharides possess agar-like structures, they were used to prepare bioactive films and expectedly, transparent and homogeneous films were obtained. The films display high water barrier properties with retained antioxidant and anti-glycation activities as above. These results expand the current knowledge of G. amansii s polysaccharides as nutraceuticals and hydrocolloids, which would benefit their applications in the food industry.
研究领域:
食品科学
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海洋生物学
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营养学
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DOI
高影响因子
MOLECULES
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2024
·Vol. 29
·期 5
·942
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IF 4.2
Jiaojiao Xue,Jianqing Su,Xueyan Wang,Rui Zhang,Xiaoli Li,Ying Li,Yi Ding,Xiuling Chu
A green extraction method was developed using deep eutectic solvent extraction for the polysaccharide fromAcanthopanax senticosus(A. senticosus). Among the eight types of DES prepared, the DES with a ratio of 1:4 L-malic acid to L-proline was found to be a suitable extraction solvent based on the extraction efficiency. The extraction parameters were optimized by Plackett–Burman and response surface methodology (RSM). The best extraction conditions were found for L-malic acid. Under the conditions of an L-malic acid/L-proline ratio of 1:4, ultrasonic power of 240 W, material–liquid ratio of 31.068 g/mL, water content of 32.364%, extraction time of 129.119 min, and extraction temperature of 60 °C, the extraction rate ofA. senticosuspolysaccharides was 35.452 ± 0.388 mg-g−1. This rate was higher than that of polysaccharides obtained by hot water extraction (13.652 ± 0.09 mg-g−1). The experimental results were best fitted by the quasi-secondary kinetic model when compared to two other kinetic models. Electron microscopic observations showed that DESs were more destructive to plant cells. The polysaccharide extracted from DESs had more monosaccharide components, a lower molecular weight, a higher antioxidant capacity, and superior anti-glycation activity compared to polysaccharides extracted from water (ASPS-PW). This study demonstrates the effectiveness of DESs in obtaining polysaccharides fromA. senticosus.
研究领域:
植物化学
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天然产物研究
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绿色化学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
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IF 8.2
Yuhang Zhu,Zhangtie Wang,Xiang Li,Siyu Chen,Daoxin Dai,Weihu Li,Binhai Shi,Baolong Wang,Guoliang Jie,Baiyi Lu
Sakura, a traditional edible flower, has been investigated for its anti-glycation potential; however, the bioactive components remain unclear. Polyphenols are recognized for their exceptional anti-glycation properties. In this study, main polyphenols were identified from seven varieties through metabolomics. 5-O-caffeoylquinic acid (5-OCA), 3-O-caffeoylquinic acid (3-OCA), and caffeic acid were confirmed as key agents through multivariate analysis, which was further validated by anti-glycation assessments. The concentrations of these polyphenols in Cerasus serrulata ‘Kanzan’ (CK) were 2.52 ± 0.08, 3.31 ± 0.18 and 2.38 ± 0.15 mg/g, respectively, contributing to its superior AGEs inhibition ratio (100 μg/mL) of 76.11 ± 0.40 %. Simulated digestion revealed that isomerization between 5-OCA and 3-OCA occurred during the gastric phase. Both compounds were metabolized to caffeic acid in the intestinal phase, contributing to a sustained anti-glycation activity of 64.74 ± 1.11 %. Overall, our study provides a theoretical basis for the development of Sakura-based functional foods targeting anti-glycation.
研究领域:
植物化学
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食品科学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2023
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IF 5.9
Weiming Chai,Qiuxia Pan,Qiuhan Bai,Yifeng Wu,Wenshuang Wei,Linjun Wang,Du Zhu
Dimocarpus longan Lour. is a valuable industrial crop for its broad applications in food , traditional Chinese medicine, and other fields. In this paper, condensed tannins were isolated from the leave of D. longan Lour., and their structure, anti-tyrosinase, anti-melanogenesis, anti-browning, anti-α-glucosidase, and anti-glycation properties were methodically clarified. The joint application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray ionization time-of-flight mass spectrometer (ESI + TOF MS), and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) confirmed that the longan leave condensed tannins (LLCTs) were polymers of procyanidins (majority) and prodelphinidins . The polymers powerfully inhibit tyrosinase (TY) with a competitive mechanism. These compounds altered the microenvironment of the binding site through hydrophobic interaction and hydrogen bond, and caused conformational modification of the enzyme by changing the polypeptide framework and secondary structure, leading to a decrease in TY activity. Cell assays further suggested that LLCTs exhibited a strong inhibition against intracellular TY activity and melanin production, and caused apoptosis in B16F10 cells. Anti-browning studies demonstrated that LLCTs suppress the browning of fresh-cut apples. Furthermore, the LLCTs exhibited an impressive inhibition on both α-glucosidase and nonenzymatic glycation . Therefore, this study confirms the potential of LLCTs as new inhibitors of tyrosinase, melanogenesis , browning, α-glucosidase, and nonenzymatic glycation, which provided a theoretical basis for the potential utilization of these polymers in the cosmetics, pharmaceutical, and food industries.
研究领域:
植物化学
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化妆品科学
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食品科学
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药理学
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 16
·2483
·
IF 4.7
Guangjing Chen,Juyan Sun,Qinghua Dai,Meiwen Sun,Peng Hu
The selection of an optimal drying method is essential for extending the shelf life and enhancing the quality ofRosa sterilisfruits. This study investigated the effects of both innovative (microwave vacuum drying and infrared drying) and traditional (freeze-drying and hot air drying) techniques on the structural characteristics and bioactivities of polysaccharides fromR. sterilisfruits (RSPs). Four different RSPs were obtained from fruits dried using these methods. Results demonstrated that the structural characteristics and bioactivities of RSPs varied significantly with the drying method. Notable differences were observed in extraction yield, total sugar, uronic acid content, monosaccharide molar ratios, molecular weight distribution, particle size, thermal stability, and microstructures of RSPs. Despite these variations, the types of constituent monosaccharides and major glycosidic linkages remained consistent across all methods. Notably, RSPs obtained via microwave vacuum drying (RSPs-MVD) showed a higher uronic acid content and lower molecular weight, and exhibited stronger in vitro antioxidant, α-glucosidase inhibitory, and antiglycation activities. These findings suggest that microwave vacuum drying is an effective pre-drying technique for extracting RSPs, making them suitable as bioactive ingredients in functional foods and pharmaceuticals for managing diabetes mellitus and its complications.
研究领域:
食品科学
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植物化学
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生物活性物质研究
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Jin Wang,Jie Li,Xiao Luo,Mei-Mei Qu Mo,Jing Feng,Wen-Bing Li,Huiling Yan,Yi-Chen Hu,Liang Zou,Ding-Tao Wu
Tartary buckwheat sprout is commonly consumed as salads and healthy vegetables, which is rich in pectic polysaccharides. Nevertheless, the knowledge about pectic polysaccharides extracted from Tartary buckwheat sprouts (TBSP) are still limited. To facilitate the potential applications of TBSP as fortified ingredients or healthy foods, the impacts of conventional hot water extraction (CHW), deep eutectic solvent (DES)-assisted extraction (DEE), and high pressure-assisted DES extraction (HPDEE) on their structural and biological properties were evaluated. Compared with the CHW method, our findings revealed that both DEE and HPDEE methods could obviously promoted the yields of TBSP and regulated their structural properties, resulting in the improvement of their biological functions. Especially, the HPDEE method could obviously decreased the molecular mass, apparent viscosity, and esterification degree of TBSP, while notably increased the uronic acids and bound flavonoids. Indeed, the HPDEE method could remarkedly enhance the antioxidant, anti-glycosylation, and immunostimulatory activities of TBSP probably via reducing its molecular mass and degree of esterification, and increasing its free uronic acids and bound flavonoids. These findings reveal that the HPDEE method has good potentials for selectively extracting distinctive TBSP with superior biological functions.
研究领域:
食品科学
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药理学
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生物化学
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植物科学
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yan Liang,Chunling Lai,Liangliang Gao,Qingna Wu,Meiling Huang,Yueming Jiang,Jun Zhang
This paper presented the initial investigation into the impact of Penicillium polonicum fermentation on the functional components and bioactivities of navel orange peel. The fermentation process resulted in a notable enhancement of the total flavonoid content (TFC), total phenolic content (TPC), antioxidant capacity, antibacterial activity, and antiglycation activity of orange peel. Eighteen flavonoids were isolated and identified from the ethanolic extract of fermented orange peel (FEE) by employing chromatographic and spectroscopic techniques. The results of the HPLC analysis showed that three flavanones (naringenin, hesperetin, and isosakuranetin) were newly produced and present in high concentrations in FEE, with hesperetin being the most abundant component (39.74 mg/g) in comparison to the ethanolic extract of unfermented orange peel (EE). The TPC, TFC, antioxidant, and antiglycation activities of FEE, as well as the enzymatic activity of β-glucosidase in the fermented orange peels, showed a gradual increase with the progression of fermentation time over a five-month period. HPLC analysis revealed that the fermentation metabolites generated by P. polonicum in rice and orange peel media exhibited distinct profiles, indicating that the fermentation of orange peel by P. polonicum did not result in the production of potential mycotoxins. The findings of this study provided a scientific basis for the high-value utilization of navel orange peels and established a reference point for the potential applications of other peel byproducts via microbial fermentation.
研究领域:
食品科学
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微生物学
·
药理学
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生物化学
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产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yapu Zhang,Chuanying Zhang,Mengfan Wang,Kexin Du,Xin Peng
The inside of the cell is closely filled with diverse biological macromolecules , which will affect various physiological activities in vivo . It is known that natural products have beneficial effects against the formation of advanced glycation end products (AGEs), but their inhibitory actions in macromolecular crowding environment are still poorly understood. Therefore, to investigate the influence of macromolecular crowding environment, the inhibitory effect of cichoric acid (CA) on the non-enzymatic glycation of bovine serum albumin (BSA) in the absence and presence of crowding reagents (PEG 2000 and PEG 4000) was studied. The results demonstrated that CA could significantly inhibit the formation of AGEs than aminoguanidine in the BSA-fructose model with or without PEG, and the inhibition effect in PBS solution was better than that in macromolecular crowding environment. Moreover, CA presented strong antioxidant capacity and trapping ability of glyoxal, and could stabilize the native structure of BSA caused by the glycation modification, which might be the primary mechanisms for its anti -glycation effect. Additionally, CA tended to bind to site I of BSA mainly through hydrogen bonds and hydrophobic interactions. These findings indicated that CA could be regarded as a high potential anti -glycation inhibitor to prevent the development of diabetic complications.
研究领域:
生物化学
·
药理学
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分子生物学
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糖尿病研究
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产品关联
DOI
高影响因子
Sustainable Chemistry and Pharmacy
·
2024
·
IF 5.5
Chunling Lai,Meiling Huang,Qin Xiong,Yan Liang,Yueming Jiang,Jun Zhang
Numerous previous studies have reported the extraction of flavonoids from orange peels, but a green and efficient approach suitable for the large-scale industrial production of flavonoids with versatile and excellent biological activities is still lacking. To tackle this issue, two easily accessible extraction methods, namely heat reflux (HR) and ultrasonication (US), coupled with four solvents (95% ethanol, 70% aqueous ethanol solution, methanol, and ethyl acetate), were used in the present study to extract flavonoids from navel orange peel, followed by chemical profile and biological activity (antioxidant, antibacterial, antiglycation, and enzyme inhibition) evaluation of all extracts obtained. The highest total phenolic content (TPC, 336.56 μmol GAE/g), total flavonoid content (TFC, 1194.31 μmol QE/g), flavonoid glycosides content (FGs, 461.82 mg/g), and the best antioxidant (IC 50 = 21.35 mg/L for ABTS assay) and α-glucosidase inhibition (IC 50 = 49.38 mg/L) activities, were found in the extract obtained by HR using ethyl acetate (EtOAc HR). However, the extract obtained by US with ethyl acetate (EtOAc US) contained the highest content of polymethoxyflavones (PMFs, 152.07 mg/g), and showed the most potent antiglycation (IC 50 = 71.26 mg/L), antibacterial (MIC = 0.078 mg/mL against MRSA), and acetylcholinesterase inhibition (AChE, IC 50 = 179.19 mg/L) activities compared to the other extracts. Furthermore, Pearson's correlation analysis was performed. This study provides simple and cost-effective methods for the preparation of orange peel extracts rich in flavonoids and versatile in biological activities, which will greatly contribute to the valorization of orange peel in food, pharmaceutical, and cosmetic industries .
研究领域:
食品科学
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药学
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化妆品科学
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生物技术
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化学工程
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产品关联
DOI
高影响因子
FOOD AND BIOPRODUCTS PROCESSING
·
2024
·
IF 3.5
Meiling Huang,Chunling Lai,Yan Liang,Qin Xiong,Chaoyun Chen,Zhiran Ju,Yueming Jiang,Jun Zhang
The fermentation of navel orange juice with lactic acid bacteria (LAB) has rarely been investigated. In this study, an autochthonous LAB strain isolated from navel orange, Lactiplantibacillus paraplantarum M23, was found to effectively ferment navel orange juice, producing a juice with enhanced functional activities and organoleptic quality. The viable bacterial count in the fermented orange juice remained consistently above 8.51 log CFU/mL throughout the entire 7-day fermentation period. The content of vitamin C (VC), total phenolic content (TPC), total flavonoid content (TFC), and hesperidin in the fermented orange juice increased by a maximum of 28.88, 1.21, 1.13, and 1.16 times, respectively, compared to the unfermented orange juice. The fermented juice exhibited significantly higher antioxidant activity than the unfermented juice, with an increase of up to 255 % and 347 % based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) assays, respectively. This may be attributed to the increase in VC and flavonoids, as demonstrated by Pearson's correlation analysis. Additionally, the fermented orange juice demonstrated improved α-glucosidase inhibition, anti-MRSA, and anti-glycation activities compared to the unfermented juice. Furthermore, the fermented juice notably reduced the levels of NO and ROS in Raw 264.7 cells without any negative impact on cell viability. The findings of this study may help in the development of nutritious and healthy fermented navel orange juice products, and serve as a reference for the production of fermented beverages from other fruits using autochthonous strains.
研究领域:
食品科学
·
微生物学
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营养学
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生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）